Reviewed, UniProtKB/Swiss-Prot Q00446 (PGLR1_COLLN)

Names and origin

Protein names

Recommended name:
    Endopolygalacturonase 1
    EC=3.2.1.15
Alternative name(s):
    Pectinase
    Clpg1

Gene names

Name:

PG1

Organism

Colletotrichum lindemuthianum (Bean anthracnose fungus) (Glomerella lindemuthiana)

Taxonomic identifier

290576 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreomycetidae incertae sedis › Glomerellaceae › Glomerella

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 4 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 26	9	Potential		PRO_0000024784
	Chain	27 – 363	337	Endopolygalacturonase 1		PRO_0000024785
Sites
	Active site	202	1	Proton donor By similarity
	Active site	224	1	By similarity
Amino acid modifications
	Glycosylation	212	1	N-linked (GlcNAc...) Potential
	Disulfide bond	29 ↔ 44		By similarity
	Disulfide bond	204 ↔ 220		By similarity
	Disulfide bond	330 ↔ 333		By similarity
	Disulfide bond	352 ↔ 363		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q00446-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A17A60386791B897	FASTA	363	36,712
	10 20 30 40 50 60 MVSYLFVLGA LASVAIASPV PELKARASCT FTDAASAIKG KASCTTIVLN NIAVPAGTTL 70 80 90 100 110 120 DMTGLKSGTH VSFSGKTTFG YKEWEGPLIS FSGSNVVIDG ASGHSIDCQG SRWWDSKGGN 130 140 150 160 170 180 GGKTKPKFFY AHSLKDSTIR GLHTLNTPVQ AFSINGAANL GVYDVSVDNS AGDSAGGHNT 190 200 210 220 230 240 DAFDVGSSTG VYISGADVKN QDDCLAVNSG TNITFTGGTC SGGHGLSIGS VGGRKDNVVK 250 260 270 280 290 300 SVSITNSKII NSDNGVRIKT VAGATGPVSD ITYSGITLSN IAKYGIVIEQ DYENGSPTGK 310 320 330 340 350 360 PTSGVPISGL TLSKISGSVS SSATPVYILC ASCTNWKWSG VSVTGGKKSS KCTGIPSGSG AAC

References

[1]

"Isolation and sequence analysis of Clpg1, a gene coding for an endopolygalacturonase of the phytopathogenic fungus Colletotrichum lindemuthianum." Centis S., Dumas B., Fournier J., Marolda M., Esquerre-Tugaye M.T. Gene 170:125-129(1996) [PubMed: 8621072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X89370 Genomic DNA. Translation: CAA61552.1.
PIR	JC4748.
3D structure databases
HSSP	HSSP built from PDB template 1IB4 based on UniProtKB O74213.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 20966.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 4 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR1_COLLN

Accession

Primary (citable) accession number: Q00446

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)