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Q00441 (CPXJ_SACEN) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-deoxyerythronolide B hydroxylase

Short name=6-DEB hydroxylase
EC=1.14.-.-
Alternative name(s):
CYPCVIIA1
Cytochrome P450 107A1
Cytochrome P450eryF
Erythromycin A biosynthesis hydrolase
Gene names
Name:eryF
Synonyms:CYP107A1
Ordered Locus Names:SACE_0730
OrganismSaccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]
Taxonomic identifier405948 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conversion of 6-deoxyerythronolide B (6-DEB) to erythronolide B (EB) by the insertion of an oxygen at the 6S position of 6-DEB.

Cofactor

Heme group.

Pathway

Antibiotic biosynthesis; erythromycin biosynthesis.

Subcellular location

Cytoplasm By similarity.

Miscellaneous

A mutated form of this enzyme leads to the formation of deoxyerythromycin A, an antibiotic with improved pharmacological properties.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4044046-deoxyerythronolide B hydroxylase
PRO_0000052220

Sites

Metal binding3511Iron (heme axial ligand)

Experimental info

Sequence conflict2611P → PDQ in CAA42927. Ref.1
Sequence conflict3021G → R in CAA42927. Ref.1

Secondary structure

........................................................................... 404
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q00441 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 257ABEFC2D88B3FE

FASTA40445,099
        10         20         30         40         50         60 
MTTVPDLESD SFHVDWYRTY AELRETAPVT PVRFLGQDAW LVTGYDEAKA ALSDLRLSSD 

        70         80         90        100        110        120 
PKKKYPGVEV EFPAYLGFPE DVRNYFATNM GTSDPPTHTR LRKLVSQEFT VRRVEAMRPR 

       130        140        150        160        170        180 
VEQITAELLD EVGDSGVVDI VDRFAHPLPI KVICELLGVD EKYRGEFGRW SSEILVMDPE 

       190        200        210        220        230        240 
RAEQRGQAAR EVVNFILDLV ERRRTEPGDD LLSALIRVQD DDDGRLSADE LTSIALVLLL 

       250        260        270        280        290        300 
AGFEASVSLI GIGTYLLLTH PDQLALVRRD PSALPNAVEE ILRYIAPPET TTRFAAEEVE 

       310        320        330        340        350        360 
IGGVAIPQYS TVLVANGAAN RDPKQFPDPH RFDVTRDTRG HLSFGQGIHF CMGRPLAKLE 

       370        380        390        400 
GEVALRALFG RFPALSLGID ADDVVWRRSL LLRGIDHLPV RLDG 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases."
Haydock S.F., Dowson J.A., Dhillon N., Roberts G.A., Cortes J., Leadlay P.F.
Mol. Gen. Genet. 230:120-128(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"An erythromycin derivative produced by targeted gene disruption in Saccharopolyspora erythraea."
Weber J.M., Leung J.O., Swanson S.J., Idler K.B., McAlpine J.B.
Science 252:114-117(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NRRL 23338.
[4]"Structure of cytochrome P450eryF involved in erythromycin biosynthesis."
Cupp-Vickery J.L., Poulos T.L.
Nat. Struct. Biol. 2:144-153(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
[5]"Crystal structures of ligand complexes of P450eryF exhibiting homotropic cooperativity."
Cupp-Vickery J., Anderson R., Hatziris Z.
Proc. Natl. Acad. Sci. U.S.A. 97:3050-3055(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
[6]"Ketoconazole-induced conformational changes in the active site of cytochrome P450eryF."
Cupp-Vickery J., Garcia C., Hofacre A., McGee-Estrada K.
J. Mol. Biol. 311:101-110(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60379 Genomic DNA. Translation: CAA42927.1.
M54983 Genomic DNA. Translation: AAA26496.1.
AM420293 Genomic DNA. Translation: CAM00071.1.
PIRS18531.
RefSeqYP_001102997.1. NC_009142.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EGYX-ray2.35A3-404[»]
1EUPX-ray2.10A3-404[»]
1JINX-ray2.30A3-404[»]
1JIOX-ray2.10A2-404[»]
1JIPX-ray2.00A2-404[»]
1OXAX-ray2.10A3-404[»]
1Z8OX-ray1.70A1-404[»]
1Z8PX-ray1.85A1-404[»]
1Z8QX-ray2.00A1-404[»]
ProteinModelPortalQ00441.
SMRQ00441. Positions 2-404.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING405948.SACE_0730.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAM00071; CAM00071; SACE_0730.
GeneID4940591.
KEGGsen:SACE_0730.
PATRIC23408848. VBISacEry28377_0744.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000243678.
KOK14366.
OMARTEEMEQ.
OrthoDBEOG6JB11P.
ProtClustDBCLSK644214.

Enzyme and pathway databases

UniPathwayUPA00240.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00359. BP450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ00441.

Entry information

Entry nameCPXJ_SACEN
AccessionPrimary (citable) accession number: Q00441
Secondary accession number(s): A4F7P7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1995
Last modified: December 11, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways