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Protein

6-deoxyerythronolide B hydroxylase

Gene

eryF

Organism
Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent conversion of 6-deoxyerythronolide B (6-DEB) to erythronolide B (EB) by the insertion of an oxygen at the 6S position of 6-DEB. Requires the participation of a ferredoxin and a ferredoxin reductase for the transfer of electrons from NADPH to the monooxygenase.2 Publications

Catalytic activityi

6-deoxyerythronolide B + NADPH + O2 = erythronolide B + NADP+ + H2O.2 Publications

Cofactori

heme3 Publications

Pathwayi: erythromycin biosynthesis

This protein is involved in the pathway erythromycin biosynthesis, which is part of Antibiotic biosynthesis.2 Publications
View all proteins of this organism that are known to be involved in the pathway erythromycin biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi351Iron (heme axial ligand)4 Publications1

GO - Molecular functioni

  • heme binding Source: UniProtKB
  • iron ion binding Source: UniProtKB
  • monooxygenase activity Source: UniProtKB
  • oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Source: InterPro

GO - Biological processi

  • erythromycin biosynthetic process Source: UniProtKB
  • oxidation-reduction process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.188. 5518.
UniPathwayiUPA00240.

Names & Taxonomyi

Protein namesi
Recommended name:
6-deoxyerythronolide B hydroxylase1 Publication (EC:1.14.13.1882 Publications)
Short name:
6-DEB hydroxylase1 Publication
Alternative name(s):
CYPCVIIA1
Cytochrome P450 107A1
Cytochrome P450eryF3 Publications
Erythromycin A biosynthesis hydroxylase
Gene namesi
Name:eryF1 Publication
Synonyms:CYP107A1
Ordered Locus Names:SACE_0730
OrganismiSaccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeSaccharopolyspora
Proteomesi
  • UP000006728 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Gene disruption leads to the accumulation of 6-deoxyerythromycin A, an antibiotic with increased stability at low pH.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000522201 – 4046-deoxyerythronolide B hydroxylaseAdd BLAST404

Interactioni

Protein-protein interaction databases

STRINGi405948.SeryN2_010100032613.

Structurei

Secondary structure

1404
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 12Combined sources3
Helixi16 – 26Combined sources11
Beta strandi28 – 34Combined sources7
Beta strandi37 – 42Combined sources6
Helixi45 – 53Combined sources9
Helixi73 – 75Combined sources3
Helixi80 – 86Combined sources7
Beta strandi87 – 89Combined sources3
Helixi90 – 92Combined sources3
Helixi97 – 106Combined sources10
Helixi111 – 116Combined sources6
Helixi118 – 130Combined sources13
Beta strandi134 – 139Combined sources6
Helixi140 – 143Combined sources4
Turni144 – 146Combined sources3
Helixi147 – 156Combined sources10
Helixi161 – 163Combined sources3
Turni164 – 166Combined sources3
Helixi167 – 175Combined sources9
Helixi179 – 181Combined sources3
Helixi182 – 205Combined sources24
Helixi211 – 217Combined sources7
Turni221 – 223Combined sources3
Helixi228 – 259Combined sources32
Helixi261 – 269Combined sources9
Helixi271 – 273Combined sources3
Helixi274 – 284Combined sources11
Beta strandi291 – 297Combined sources7
Beta strandi299 – 301Combined sources3
Beta strandi304 – 306Combined sources3
Beta strandi311 – 314Combined sources4
Helixi316 – 319Combined sources4
Turni323 – 325Combined sources3
Beta strandi326 – 328Combined sources3
Beta strandi334 – 336Combined sources3
Beta strandi342 – 344Combined sources3
Helixi347 – 349Combined sources3
Helixi354 – 371Combined sources18
Beta strandi376 – 379Combined sources4
Helixi381 – 383Combined sources3
Beta strandi390 – 392Combined sources3
Beta strandi399 – 401Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EGYX-ray2.35A3-404[»]
1EUPX-ray2.10A3-404[»]
1JINX-ray2.30A3-404[»]
1JIOX-ray2.10A2-404[»]
1JIPX-ray2.00A2-404[»]
1OXAX-ray2.10A3-404[»]
1Z8OX-ray1.70A1-404[»]
1Z8PX-ray1.85A1-404[»]
1Z8QX-ray2.00A1-404[»]
ProteinModelPortaliQ00441.
SMRiQ00441.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00441.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiENOG4105Q56. Bacteria.
ENOG410XT87. LUCA.
HOGENOMiHOG000243678.
KOiK14366.
OMAiIKVICEL.
OrthoDBiPOG091H0API.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00359. BP450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTVPDLESD SFHVDWYRTY AELRETAPVT PVRFLGQDAW LVTGYDEAKA
60 70 80 90 100
ALSDLRLSSD PKKKYPGVEV EFPAYLGFPE DVRNYFATNM GTSDPPTHTR
110 120 130 140 150
LRKLVSQEFT VRRVEAMRPR VEQITAELLD EVGDSGVVDI VDRFAHPLPI
160 170 180 190 200
KVICELLGVD EKYRGEFGRW SSEILVMDPE RAEQRGQAAR EVVNFILDLV
210 220 230 240 250
ERRRTEPGDD LLSALIRVQD DDDGRLSADE LTSIALVLLL AGFEASVSLI
260 270 280 290 300
GIGTYLLLTH PDQLALVRRD PSALPNAVEE ILRYIAPPET TTRFAAEEVE
310 320 330 340 350
IGGVAIPQYS TVLVANGAAN RDPKQFPDPH RFDVTRDTRG HLSFGQGIHF
360 370 380 390 400
CMGRPLAKLE GEVALRALFG RFPALSLGID ADDVVWRRSL LLRGIDHLPV

RLDG
Length:404
Mass (Da):45,099
Last modified:February 1, 1995 - v2
Checksum:i257ABEFC2D88B3FE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti261P → PDQ in CAA42927 (PubMed:1840640).Curated1
Sequence conflicti302G → R in CAA42927 (PubMed:1840640).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60379 Genomic DNA. Translation: CAA42927.1.
M54983 Genomic DNA. Translation: AAA26496.1.
AM420293 Genomic DNA. Translation: CAM00071.1.
PIRiS18531.
RefSeqiWP_009950397.1. NZ_ABFV01000100.1.

Genome annotation databases

EnsemblBacteriaiCAM00071; CAM00071; SACE_0730.
KEGGisen:SACE_0730.
PATRICi23408848. VBISacEry28377_0744.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60379 Genomic DNA. Translation: CAA42927.1.
M54983 Genomic DNA. Translation: AAA26496.1.
AM420293 Genomic DNA. Translation: CAM00071.1.
PIRiS18531.
RefSeqiWP_009950397.1. NZ_ABFV01000100.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EGYX-ray2.35A3-404[»]
1EUPX-ray2.10A3-404[»]
1JINX-ray2.30A3-404[»]
1JIOX-ray2.10A2-404[»]
1JIPX-ray2.00A2-404[»]
1OXAX-ray2.10A3-404[»]
1Z8OX-ray1.70A1-404[»]
1Z8PX-ray1.85A1-404[»]
1Z8QX-ray2.00A1-404[»]
ProteinModelPortaliQ00441.
SMRiQ00441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405948.SeryN2_010100032613.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAM00071; CAM00071; SACE_0730.
KEGGisen:SACE_0730.
PATRICi23408848. VBISacEry28377_0744.

Phylogenomic databases

eggNOGiENOG4105Q56. Bacteria.
ENOG410XT87. LUCA.
HOGENOMiHOG000243678.
KOiK14366.
OMAiIKVICEL.
OrthoDBiPOG091H0API.

Enzyme and pathway databases

UniPathwayiUPA00240.
BRENDAi1.14.13.188. 5518.

Miscellaneous databases

EvolutionaryTraceiQ00441.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00359. BP450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCPXJ_SACEN
AccessioniPrimary (citable) accession number: Q00441
Secondary accession number(s): A4F7P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The bound substrate forms no direct hydrogen bonds with the protein.2 Publications

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.