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Q00441

- CPXJ_SACEN

UniProt

Q00441 - CPXJ_SACEN

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Protein

6-deoxyerythronolide B hydroxylase

Gene

eryF

Organism
Saccharopolyspora erythraea (strain NRRL 23338)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent conversion of 6-deoxyerythronolide B (6-DEB) to erythronolide B (EB) by the insertion of an oxygen at the 6S position of 6-DEB. Requires the participation of a ferredoxin and a ferredoxin reductase for the transfer of electrons from NADPH to the monooxygenase.2 Publications

Catalytic activityi

6-deoxyerythronolide B + NADPH + O2 = erythronolide B + NADP+ + H2O.2 Publications

Cofactori

Heme group.3 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi351 – 3511Iron (heme axial ligand)4 Publications

GO - Molecular functioni

  1. heme binding Source: UniProtKB
  2. iron ion binding Source: UniProtKB
  3. monooxygenase activity Source: UniProtKB
  4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Source: InterPro

GO - Biological processi

  1. erythromycin biosynthetic process Source: UniProtKB
  2. oxidation-reduction process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00240.

Names & Taxonomyi

Protein namesi
Recommended name:
6-deoxyerythronolide B hydroxylase1 Publication (EC:1.14.13.1882 Publications)
Short name:
6-DEB hydroxylase1 Publication
Alternative name(s):
CYPCVIIA1
Cytochrome P450 107A1
Cytochrome P450eryF3 Publications
Erythromycin A biosynthesis hydroxylase
Gene namesi
Name:eryF1 Publication
Synonyms:CYP107A1
Ordered Locus Names:SACE_0730
OrganismiSaccharopolyspora erythraea (strain NRRL 23338)
Taxonomic identifieri405948 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeSaccharopolyspora
ProteomesiUP000006728: Chromosome

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Gene disruption leads to the accumulation of 6-deoxyerythromycin A, an antibiotic with increased stability at low pH.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4044046-deoxyerythronolide B hydroxylasePRO_0000052220Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi405948.SACE_0730.

Structurei

Secondary structure

1
404
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 123
Helixi16 – 2611
Beta strandi28 – 347
Beta strandi37 – 426
Helixi45 – 539
Helixi73 – 753
Helixi80 – 867
Beta strandi87 – 893
Helixi90 – 923
Helixi97 – 10610
Helixi111 – 1166
Helixi118 – 13013
Beta strandi134 – 1396
Helixi140 – 1434
Turni144 – 1463
Helixi147 – 15610
Helixi161 – 1633
Turni164 – 1663
Helixi167 – 1759
Helixi179 – 1813
Helixi182 – 20524
Helixi211 – 2177
Turni221 – 2233
Helixi228 – 25932
Helixi261 – 2699
Helixi271 – 2733
Helixi274 – 28411
Beta strandi291 – 2977
Beta strandi299 – 3013
Beta strandi304 – 3063
Beta strandi311 – 3144
Helixi316 – 3194
Turni323 – 3253
Beta strandi326 – 3283
Beta strandi334 – 3363
Beta strandi342 – 3443
Helixi347 – 3493
Helixi354 – 37118
Beta strandi376 – 3794
Helixi381 – 3833
Beta strandi390 – 3923
Beta strandi399 – 4013

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EGYX-ray2.35A3-404[»]
1EUPX-ray2.10A3-404[»]
1JINX-ray2.30A3-404[»]
1JIOX-ray2.10A2-404[»]
1JIPX-ray2.00A2-404[»]
1OXAX-ray2.10A3-404[»]
1Z8OX-ray1.70A1-404[»]
1Z8PX-ray1.85A1-404[»]
1Z8QX-ray2.00A1-404[»]
ProteinModelPortaliQ00441.
SMRiQ00441. Positions 2-404.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00441.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000243678.
KOiK14366.
OMAiIKVICEL.
OrthoDBiEOG6JB11P.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00359. BP450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00441-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTVPDLESD SFHVDWYRTY AELRETAPVT PVRFLGQDAW LVTGYDEAKA
60 70 80 90 100
ALSDLRLSSD PKKKYPGVEV EFPAYLGFPE DVRNYFATNM GTSDPPTHTR
110 120 130 140 150
LRKLVSQEFT VRRVEAMRPR VEQITAELLD EVGDSGVVDI VDRFAHPLPI
160 170 180 190 200
KVICELLGVD EKYRGEFGRW SSEILVMDPE RAEQRGQAAR EVVNFILDLV
210 220 230 240 250
ERRRTEPGDD LLSALIRVQD DDDGRLSADE LTSIALVLLL AGFEASVSLI
260 270 280 290 300
GIGTYLLLTH PDQLALVRRD PSALPNAVEE ILRYIAPPET TTRFAAEEVE
310 320 330 340 350
IGGVAIPQYS TVLVANGAAN RDPKQFPDPH RFDVTRDTRG HLSFGQGIHF
360 370 380 390 400
CMGRPLAKLE GEVALRALFG RFPALSLGID ADDVVWRRSL LLRGIDHLPV

RLDG
Length:404
Mass (Da):45,099
Last modified:February 1, 1995 - v2
Checksum:i257ABEFC2D88B3FE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti261 – 2611P → PDQ in CAA42927. (PubMed:1840640)Curated
Sequence conflicti302 – 3021G → R in CAA42927. (PubMed:1840640)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60379 Genomic DNA. Translation: CAA42927.1.
M54983 Genomic DNA. Translation: AAA26496.1.
AM420293 Genomic DNA. Translation: CAM00071.1.
PIRiS18531.
RefSeqiWP_009950397.1. NZ_ABFV01000100.1.
YP_001102997.1. NC_009142.1.

Genome annotation databases

EnsemblBacteriaiCAM00071; CAM00071; SACE_0730.
GeneIDi4940591.
KEGGisen:SACE_0730.
PATRICi23408848. VBISacEry28377_0744.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60379 Genomic DNA. Translation: CAA42927.1 .
M54983 Genomic DNA. Translation: AAA26496.1 .
AM420293 Genomic DNA. Translation: CAM00071.1 .
PIRi S18531.
RefSeqi WP_009950397.1. NZ_ABFV01000100.1.
YP_001102997.1. NC_009142.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EGY X-ray 2.35 A 3-404 [» ]
1EUP X-ray 2.10 A 3-404 [» ]
1JIN X-ray 2.30 A 3-404 [» ]
1JIO X-ray 2.10 A 2-404 [» ]
1JIP X-ray 2.00 A 2-404 [» ]
1OXA X-ray 2.10 A 3-404 [» ]
1Z8O X-ray 1.70 A 1-404 [» ]
1Z8P X-ray 1.85 A 1-404 [» ]
1Z8Q X-ray 2.00 A 1-404 [» ]
ProteinModelPortali Q00441.
SMRi Q00441. Positions 2-404.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 405948.SACE_0730.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAM00071 ; CAM00071 ; SACE_0730 .
GeneIDi 4940591.
KEGGi sen:SACE_0730.
PATRICi 23408848. VBISacEry28377_0744.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000243678.
KOi K14366.
OMAi IKVICEL.
OrthoDBi EOG6JB11P.

Enzyme and pathway databases

UniPathwayi UPA00240 .

Miscellaneous databases

EvolutionaryTracei Q00441.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00359. BP450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases."
    Haydock S.F., Dowson J.A., Dhillon N., Roberts G.A., Cortes J., Leadlay P.F.
    Mol. Gen. Genet. 230:120-128(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "An erythromycin derivative produced by targeted gene disruption in Saccharopolyspora erythraea."
    Weber J.M., Leung J.O., Swanson S.J., Idler K.B., McAlpine J.B.
    Science 252:114-117(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, PATHWAY.
  3. "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."
    Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.
    Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NRRL 23338.
  4. "Characterization of Saccharopolyspora erythraea cytochrome P-450 genes and enzymes, including 6-deoxyerythronolide B hydroxylase."
    Andersen J.F., Hutchinson C.R.
    J. Bacteriol. 174:725-735(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY.
  5. "Structure of cytochrome P450eryF involved in erythromycin biosynthesis."
    Cupp-Vickery J.L., Poulos T.L.
    Nat. Struct. Biol. 2:144-153(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 3-404 IN COMPLEX WITH HEME AND 6-DEOXYERYTHRONOLIDE B, COFACTOR.
  6. "Crystal structures of ligand complexes of P450eryF exhibiting homotropic cooperativity."
    Cupp-Vickery J., Anderson R., Hatziris Z.
    Proc. Natl. Acad. Sci. U.S.A. 97:3050-3055(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 3-404 IN COMPLEX WITH HEME, COFACTOR.
  7. "Ketoconazole-induced conformational changes in the active site of cytochrome P450eryF."
    Cupp-Vickery J., Garcia C., Hofacre A., McGee-Estrada K.
    J. Mol. Biol. 311:101-110(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 2-404 IN COMPLEX WITH HEME, COFACTOR.
  8. "Crystal structures of the ferrous dioxygen complex of wild-type cytochrome P450eryF and its mutants, A245S and A245T: investigation of the proton transfer system in P450eryF."
    Nagano S., Cupp-Vickery J.R., Poulos T.L.
    J. Biol. Chem. 280:22102-22107(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH HEME AND 6-DEOXYERYTHRONOLIDE B, COFACTOR.

Entry informationi

Entry nameiCPXJ_SACEN
AccessioniPrimary (citable) accession number: Q00441
Secondary accession number(s): A4F7P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The bound substrate forms no direct hydrogen bonds with the protein.2 Publications

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3