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Protein

Polypyrimidine tract-binding protein 1

Gene

Ptbp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (By similarity).By similarity

GO - Molecular functioni

  • mRNA binding Source: RGD
  • nucleotide binding Source: InterPro
  • regulatory region RNA binding Source: RGD
  • sequence-specific DNA binding Source: RGD
  • single-stranded DNA binding Source: RGD
  • single-stranded DNA endodeoxyribonuclease activity Source: RGD

GO - Biological processi

  • 3'-UTR-mediated mRNA stabilization Source: RGD
  • cardiac ventricle development Source: RGD
  • mRNA processing Source: RGD
  • negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • negative regulation of muscle cell differentiation Source: UniProtKB
  • nuclear-transcribed mRNA catabolic process Source: RGD
  • positive regulation of insulin secretion Source: RGD
  • positive regulation of neuron projection development Source: RGD
  • positive regulation of secretory granule organization Source: RGD
  • positive regulation of translation Source: RGD
  • regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  • RNA splicing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polypyrimidine tract-binding protein 1
Short name:
PTB
Alternative name(s):
Heterogeneous nuclear ribonucleoprotein I
Short name:
hnRNP I
Pyrimidine-binding protein
Short name:
PYBP
Gene namesi
Name:Ptbp1
Synonyms:Ptb, Pybp, Tbfii
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi62047. Ptbp1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • neuron projection terminus Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000817401 – 555Polypyrimidine tract-binding protein 1Add BLAST555

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei16PhosphoserineBy similarity1
Modified residuei126PhosphotyrosineBy similarity1
Modified residuei137PhosphothreonineBy similarity1
Modified residuei140PhosphoserineBy similarity1
Modified residuei457PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ00438.
PeptideAtlasiQ00438.
PRIDEiQ00438.

PTM databases

iPTMnetiQ00438.
PhosphoSitePlusiQ00438.

Interactioni

Subunit structurei

Monomer. Part of a ternary complex containing KHSRP, PTBP1, PTBP2 and HNRPH1. Interacts with RAVER1 and SFPQ (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043256.

Structurei

3D structure databases

ProteinModelPortaliQ00438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 142RRM 1PROSITE-ProRule annotationAdd BLAST85
Domaini183 – 259RRM 2PROSITE-ProRule annotationAdd BLAST77
Domaini361 – 435RRM 3PROSITE-ProRule annotationAdd BLAST75
Domaini478 – 553RRM 4PROSITE-ProRule annotationAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi340 – 347Poly-Ala8

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1190. Eukaryota.
ENOG410XPMZ. LUCA.
HOGENOMiHOG000231699.
HOVERGENiHBG069548.
InParanoidiQ00438.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform PYBP2 (identifier: Q00438-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGIVPDIAV GTKRGSDELF STCVSNGPFI MSSSASAANG NDSKKFKGDN
60 70 80 90 100
RSTGVPSRVI HVRKLPSDVT EGEVISLGLP FGKVTNLLML KGKNQAFIEM
110 120 130 140 150
STEEAANTMV NYYTSVAPVL RGQPIYIQFS NHKELKTDSS PNQARAQAAL
160 170 180 190 200
QAVNSVQSGN LALAASAAAV DAGMAMAGQS PVLRIIVENL FYPVTLDVLH
210 220 230 240 250
QIFSKFGTVL KIITFTKNNQ FQALLQYADP VSAQHAKLSL DGQNIYNACC
260 270 280 290 300
TLRIDFSKLT SLNVKYNNDK SRDYTRPDLP SGDSQPSLDQ TMAAAFGAPG
310 320 330 340 350
IMSASPYAGA VPSHLCHPSR AGLSVPNVHG ALAPLAIPSA AAAAAAAGRI
360 370 380 390 400
AIPGLAGAGN SVLLVSNLNP ERVTPQSLFI LFGVYGDVQR VKILFNKKEN
410 420 430 440 450
ALVEMADGSQ AQLAMSHLNG HKLHGKSVRI TLSKHQSVQL PREGQEDQGL
460 470 480 490 500
TKDYGSSPLH RFKKPGSKNF QNIFPPSATL HLSNIPPSVS EDDLKSLFSS
510 520 530 540 550
NGGVVKGFKF FQKDRKMALI QMGSVEEAVQ ALIELHNHDL GENHHLRVSF

SKSTI
Length:555
Mass (Da):59,354
Last modified:April 1, 1993 - v1
Checksum:iE73F22B54467117F
GO
Isoform PYBP1 (identifier: Q00438-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     297-321: Missing.

Show »
Length:530
Mass (Da):56,937
Checksum:i6B264A5887FF80A7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57S → T in CAA52653 (Ref. 2) Curated1
Sequence conflicti164A → R in CAA52653 (Ref. 2) Curated1
Sequence conflicti311 – 320VPSHLCHPSR → GFPPTFAIPQA in CAA52653 (Ref. 2) Curated10
Sequence conflicti542 – 549ENHHLRVS → LGDNHHKR AA sequence (PubMed:1681508).Curated8

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005803297 – 321Missing in isoform PYBP1. CuratedAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60789 mRNA. Translation: CAA43202.1.
X60790 mRNA. Translation: CAA43203.1.
X74565 mRNA. Translation: CAA52653.1.
PIRiS15552.
S36629.
UniGeneiRn.64440.

Genome annotation databases

UCSCiRGD:62047. rat. [Q00438-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60789 mRNA. Translation: CAA43202.1.
X60790 mRNA. Translation: CAA43203.1.
X74565 mRNA. Translation: CAA52653.1.
PIRiS15552.
S36629.
UniGeneiRn.64440.

3D structure databases

ProteinModelPortaliQ00438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043256.

PTM databases

iPTMnetiQ00438.
PhosphoSitePlusiQ00438.

Proteomic databases

PaxDbiQ00438.
PeptideAtlasiQ00438.
PRIDEiQ00438.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:62047. rat. [Q00438-1]

Organism-specific databases

RGDi62047. Ptbp1.

Phylogenomic databases

eggNOGiKOG1190. Eukaryota.
ENOG410XPMZ. LUCA.
HOGENOMiHOG000231699.
HOVERGENiHBG069548.
InParanoidiQ00438.

Miscellaneous databases

PROiQ00438.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTBP1_RAT
AccessioniPrimary (citable) accession number: Q00438
Secondary accession number(s): Q63568
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.