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Q00438 (PTBP1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polypyrimidine tract-binding protein 1

Short name=PTB
Alternative name(s):
Heterogeneous nuclear ribonucleoprotein I
Short name=hnRNP I
Pyrimidine-binding protein
Short name=PYBP
Gene names
Name:Ptbp1
Synonyms:Ptb, Pybp, Tbfii
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length555 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 By similarity.

Subunit structure

Monomer. Part of a ternary complex containing KHSRP, PTBP1, PTBP2 and HNRPH1. Interacts with RAVER1 and SFPQ By similarity.

Subcellular location

Nucleus.

Sequence similarities

Contains 4 RRM (RNA recognition motif) domains.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform PYBP2 (identifier: Q00438-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform PYBP1 (identifier: Q00438-2)

The sequence of this isoform differs from the canonical sequence as follows:
     297-321: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 555555Polypyrimidine tract-binding protein 1
PRO_0000081740

Regions

Domain58 – 14285RRM 1
Domain183 – 25977RRM 2
Domain361 – 43575RRM 3
Domain478 – 55376RRM 4
Compositional bias340 – 3478Poly-Ala

Amino acid modifications

Modified residue11N-acetylmethionine Ref.3
Modified residue161Phosphoserine By similarity
Modified residue1371Phosphothreonine By similarity
Modified residue1401Phosphoserine By similarity
Modified residue4571Phosphoserine By similarity

Natural variations

Alternative sequence297 – 32125Missing in isoform PYBP1.
VSP_005803

Experimental info

Sequence conflict571S → T in CAA52653. Ref.2
Sequence conflict1641A → R in CAA52653. Ref.2
Sequence conflict311 – 32010VPSHLCHPSR → GFPPTFAIPQA in CAA52653. Ref.2
Sequence conflict542 – 5498ENHHLRVS → LGDNHHKR AA sequence Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform PYBP2 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: E73F22B54467117F

FASTA55559,354
        10         20         30         40         50         60 
MDGIVPDIAV GTKRGSDELF STCVSNGPFI MSSSASAANG NDSKKFKGDN RSTGVPSRVI 

        70         80         90        100        110        120 
HVRKLPSDVT EGEVISLGLP FGKVTNLLML KGKNQAFIEM STEEAANTMV NYYTSVAPVL 

       130        140        150        160        170        180 
RGQPIYIQFS NHKELKTDSS PNQARAQAAL QAVNSVQSGN LALAASAAAV DAGMAMAGQS 

       190        200        210        220        230        240 
PVLRIIVENL FYPVTLDVLH QIFSKFGTVL KIITFTKNNQ FQALLQYADP VSAQHAKLSL 

       250        260        270        280        290        300 
DGQNIYNACC TLRIDFSKLT SLNVKYNNDK SRDYTRPDLP SGDSQPSLDQ TMAAAFGAPG 

       310        320        330        340        350        360 
IMSASPYAGA VPSHLCHPSR AGLSVPNVHG ALAPLAIPSA AAAAAAAGRI AIPGLAGAGN 

       370        380        390        400        410        420 
SVLLVSNLNP ERVTPQSLFI LFGVYGDVQR VKILFNKKEN ALVEMADGSQ AQLAMSHLNG 

       430        440        450        460        470        480 
HKLHGKSVRI TLSKHQSVQL PREGQEDQGL TKDYGSSPLH RFKKPGSKNF QNIFPPSATL 

       490        500        510        520        530        540 
HLSNIPPSVS EDDLKSLFSS NGGVVKGFKF FQKDRKMALI QMGSVEEAVQ ALIELHNHDL 

       550 
GENHHLRVSF SKSTI 

« Hide

Isoform PYBP1 [UniParc].

Checksum: 6B264A5887FF80A7
Show »

FASTA53056,937

References

[1]"Cloning and sequencing of PYBP, a pyrimidine-rich specific single strand DNA-binding protein."
Brunel F., Alzari P.M., Ferrara P., Zakin M.M.
Nucleic Acids Res. 19:5237-5245(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 185-205; 348-365 AND 517-549.
Tissue: Hepatoma.
[2]Sengupta P.
Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]Bienvenut W.V., von Kriegsheim A.F., Kolch W.
Submitted (AUG-2006) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 1-13; 84-91; 122-133; 435-452 AND 496-506, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Pheochromocytoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60789 mRNA. Translation: CAA43202.1.
X60790 mRNA. Translation: CAA43203.1.
X74565 mRNA. Translation: CAA52653.1.
PIRS15552.
S36629.
UniGeneRn.64440.

3D structure databases

ProteinModelPortalQ00438.
SMRQ00438. Positions 48-145, 171-297, 348-555.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248137. 1 interaction.

PTM databases

PhosphoSiteQ00438.

Proteomic databases

PaxDbQ00438.
PRIDEQ00438.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:62047. rat. [Q00438-1]

Organism-specific databases

RGD62047. Ptbp1.

Phylogenomic databases

eggNOGNOG263741.
HOGENOMHOG000231699.
HOVERGENHBG069548.
InParanoidQ00438.

Gene expression databases

GenevestigatorQ00438.

Family and domain databases

Gene3D3.30.70.330. 4 hits.
InterProIPR006536. HnRNP-L_PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
SMARTSM00360. RRM. 4 hits.
[Graphical view]
TIGRFAMsTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEPS50102. RRM. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio609388.

Entry information

Entry namePTBP1_RAT
AccessionPrimary (citable) accession number: Q00438
Secondary accession number(s): Q63568
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families