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Q00438

- PTBP1_RAT

UniProt

Q00438 - PTBP1_RAT

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Protein

Polypyrimidine tract-binding protein 1

Gene

Ptbp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (By similarity).By similarity

GO - Molecular functioni

  1. endonuclease activity Source: RGD
  2. nucleotide binding Source: InterPro
  3. RNA binding Source: RGD
  4. single-stranded DNA binding Source: RGD

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
  3. negative regulation of muscle cell differentiation Source: UniProtKB
  4. nucleic acid phosphodiester bond hydrolysis Source: GOC
  5. regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  6. RNA splicing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polypyrimidine tract-binding protein 1
Short name:
PTB
Alternative name(s):
Heterogeneous nuclear ribonucleoprotein I
Short name:
hnRNP I
Pyrimidine-binding protein
Short name:
PYBP
Gene namesi
Name:Ptbp1
Synonyms:Ptb, Pybp, Tbfii
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi62047. Ptbp1.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 555555Polypyrimidine tract-binding protein 1PRO_0000081740Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei16 – 161PhosphoserineBy similarity
Modified residuei137 – 1371PhosphothreonineBy similarity
Modified residuei140 – 1401PhosphoserineBy similarity
Modified residuei457 – 4571PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ00438.
PRIDEiQ00438.

PTM databases

PhosphoSiteiQ00438.

Expressioni

Gene expression databases

GenevestigatoriQ00438.

Interactioni

Subunit structurei

Monomer. Part of a ternary complex containing KHSRP, PTBP1, PTBP2 and HNRPH1. Interacts with RAVER1 and SFPQ (By similarity).By similarity

Protein-protein interaction databases

BioGridi248137. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ00438.
SMRiQ00438. Positions 48-145, 171-297, 348-555.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 14285RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini183 – 25977RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini361 – 43575RRM 3PROSITE-ProRule annotationAdd
BLAST
Domaini478 – 55376RRM 4PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi340 – 3478Poly-Ala

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG263741.
HOGENOMiHOG000231699.
HOVERGENiHBG069548.
InParanoidiQ00438.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L_PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform PYBP2 (identifier: Q00438-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGIVPDIAV GTKRGSDELF STCVSNGPFI MSSSASAANG NDSKKFKGDN
60 70 80 90 100
RSTGVPSRVI HVRKLPSDVT EGEVISLGLP FGKVTNLLML KGKNQAFIEM
110 120 130 140 150
STEEAANTMV NYYTSVAPVL RGQPIYIQFS NHKELKTDSS PNQARAQAAL
160 170 180 190 200
QAVNSVQSGN LALAASAAAV DAGMAMAGQS PVLRIIVENL FYPVTLDVLH
210 220 230 240 250
QIFSKFGTVL KIITFTKNNQ FQALLQYADP VSAQHAKLSL DGQNIYNACC
260 270 280 290 300
TLRIDFSKLT SLNVKYNNDK SRDYTRPDLP SGDSQPSLDQ TMAAAFGAPG
310 320 330 340 350
IMSASPYAGA VPSHLCHPSR AGLSVPNVHG ALAPLAIPSA AAAAAAAGRI
360 370 380 390 400
AIPGLAGAGN SVLLVSNLNP ERVTPQSLFI LFGVYGDVQR VKILFNKKEN
410 420 430 440 450
ALVEMADGSQ AQLAMSHLNG HKLHGKSVRI TLSKHQSVQL PREGQEDQGL
460 470 480 490 500
TKDYGSSPLH RFKKPGSKNF QNIFPPSATL HLSNIPPSVS EDDLKSLFSS
510 520 530 540 550
NGGVVKGFKF FQKDRKMALI QMGSVEEAVQ ALIELHNHDL GENHHLRVSF

SKSTI
Length:555
Mass (Da):59,354
Last modified:April 1, 1993 - v1
Checksum:iE73F22B54467117F
GO
Isoform PYBP1 (identifier: Q00438-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     297-321: Missing.

Show »
Length:530
Mass (Da):56,937
Checksum:i6B264A5887FF80A7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571S → T in CAA52653. 1 PublicationCurated
Sequence conflicti164 – 1641A → R in CAA52653. 1 PublicationCurated
Sequence conflicti311 – 32010VPSHLCHPSR → GFPPTFAIPQA in CAA52653. 1 PublicationCurated
Sequence conflicti542 – 5498ENHHLRVS → LGDNHHKR AA sequence (PubMed:1681508)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei297 – 32125Missing in isoform PYBP1. CuratedVSP_005803Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60789 mRNA. Translation: CAA43202.1.
X60790 mRNA. Translation: CAA43203.1.
X74565 mRNA. Translation: CAA52653.1.
PIRiS15552.
S36629.
UniGeneiRn.64440.

Genome annotation databases

UCSCiRGD:62047. rat. [Q00438-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60789 mRNA. Translation: CAA43202.1 .
X60790 mRNA. Translation: CAA43203.1 .
X74565 mRNA. Translation: CAA52653.1 .
PIRi S15552.
S36629.
UniGenei Rn.64440.

3D structure databases

ProteinModelPortali Q00438.
SMRi Q00438. Positions 48-145, 171-297, 348-555.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248137. 1 interaction.

PTM databases

PhosphoSitei Q00438.

Proteomic databases

PaxDbi Q00438.
PRIDEi Q00438.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:62047. rat. [Q00438-1 ]

Organism-specific databases

RGDi 62047. Ptbp1.

Phylogenomic databases

eggNOGi NOG263741.
HOGENOMi HOG000231699.
HOVERGENi HBG069548.
InParanoidi Q00438.

Miscellaneous databases

NextBioi 609388.

Gene expression databases

Genevestigatori Q00438.

Family and domain databases

Gene3Di 3.30.70.330. 4 hits.
InterProi IPR006536. HnRNP-L_PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view ]
SMARTi SM00360. RRM. 4 hits.
[Graphical view ]
TIGRFAMsi TIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEi PS50102. RRM. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of PYBP, a pyrimidine-rich specific single strand DNA-binding protein."
    Brunel F., Alzari P.M., Ferrara P., Zakin M.M.
    Nucleic Acids Res. 19:5237-5245(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 185-205; 348-365 AND 517-549.
    Tissue: Hepatoma.
  2. Sengupta P.
    Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Bienvenut W.V., von Kriegsheim A.F., Kolch W.
    Submitted (AUG-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1-13; 84-91; 122-133; 435-452 AND 496-506, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Pheochromocytoma.

Entry informationi

Entry nameiPTBP1_RAT
AccessioniPrimary (citable) accession number: Q00438
Secondary accession number(s): Q63568
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 29, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3