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Q00433

- PHE3_RHDS2

UniProt

Q00433 - PHE3_RHDS2

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Protein

Phycoerythrin alpha-3 chain, chloroplastic

Gene

cpeA3

Organism
Rhodomonas sp. (strain CS 24) (Chroomonas sp. (strain CS24))
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Light-harvesting photosynthetic tetrapyrrole chromophore-protein from the phycobiliprotein complex.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 71115,16-dihydrobiliverdin chromophore (covalent; via 1 link)2 Publications
Binding sitei73 – 73115,16-dihydrobiliverdin chromophore2 Publications
Binding sitei93 – 93115,16-dihydrobiliverdin chromophore2 Publications

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB-KW
  2. photosynthesis Source: UniProtKB-KW
  3. protein-chromophore linkage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Bile pigment, Chromophore

Names & Taxonomyi

Protein namesi
Recommended name:
Phycoerythrin alpha-3 chain, chloroplastic
Gene namesi
Name:cpeA3
OrganismiRhodomonas sp. (strain CS 24) (Chroomonas sp. (strain CS24))
Taxonomic identifieri79257 [NCBI]
Taxonomic lineageiEukaryotaCryptophytaPyrenomonadalesPyrenomonadaceaeRhodomonas

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
  2. phycobilisome Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5252ChloroplastAdd
BLAST
Chaini53 – 12876Phycoerythrin alpha-3 chain, chloroplasticPRO_0000002828Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 5615-hydroxylysine2 Publications

Post-translational modificationi

Contains one covalently linked 15,16-dihydrobiliverdin chromophore.

Keywords - PTMi

Hydroxylation

Interactioni

Subunit structurei

Heterotetramer of 2 different alpha chains and 2 identical beta chains. The subunit composition could comprise of any combination of 2 out of 4 different alpha units with an invariant beta unit.2 Publications

Structurei

Secondary structure

1
128
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi59 – 679
Helixi86 – 894
Beta strandi90 – 989
Helixi103 – 11311
Beta strandi120 – 1223

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QGWX-ray1.63A53-128[»]
1XF6X-ray1.10A53-128[»]
1XG0X-ray0.97A53-128[»]
ProteinModelPortaliQ00433.
SMRiQ00433. Positions 53-128.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00433.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni77 – 78215,16-dihydrobiliverdin chromophore binding

Sequence similaritiesi

Belongs to the phycoerythrin family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.90.510.10. 1 hit.
InterProiIPR011070. Globular_prot_asu/bsu.
IPR004228. Phycoerythr_a_core.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF02972. Phycoerythr_ab. 1 hit.
[Graphical view]
ProDomiPD019398. Phycoerythr_a/b_core. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56568. SSF56568. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00433-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFAKTLASLA VIGSAAAYVP MMSMDMGRRE VVQAGAAAAA VTPFLSGAPA
60 70 80 90 100
GAAMDKSAKA PQITIFDHRG CSRAPKESTG GKAGGQDDEM MVKVASTKVT
110 120
VSESDAAKKL QEFITFEKGI DGPFTSKN
Length:128
Mass (Da):13,175
Last modified:May 27, 2002 - v2
Checksum:i44B1ECFD62C12732
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ006994 Genomic DNA. Translation: CAA07415.2.
PIRiS12900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ006994 Genomic DNA. Translation: CAA07415.2 .
PIRi S12900.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1QGW X-ray 1.63 A 53-128 [» ]
1XF6 X-ray 1.10 A 53-128 [» ]
1XG0 X-ray 0.97 A 53-128 [» ]
ProteinModelPortali Q00433.
SMRi Q00433. Positions 53-128.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q00433.

Family and domain databases

Gene3Di 3.90.510.10. 1 hit.
InterProi IPR011070. Globular_prot_asu/bsu.
IPR004228. Phycoerythr_a_core.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF02972. Phycoerythr_ab. 1 hit.
[Graphical view ]
ProDomi PD019398. Phycoerythr_a/b_core. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF56568. SSF56568. 1 hit.
PROSITEi PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A genomic clone encoding a cryptophyte phycoerythrin alpha-subunit. Evidence for three alpha-subunits and an N-terminal membrane transit sequence."
    Jenkins J., Hiller R.G., Speirs J., Godovac-Zimmermann J.
    FEBS Lett. 273:191-194(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nuclear-encoded genes of phycoerythrin alpha subunits."
    Hiller R.G., Howe C.E.
    Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION TO 62.
  3. "Evolution of a light-harvesting protein by addition of new subunits and rearrangement of conserved elements: crystal structure of a cryptophyte phycoerythrin at 1.63-A resolution."
    Wilk K.E., Harrop S.J., Jankova L., Edler D., Keenan G., Sharples F., Hiller R.G., Curmi P.M.
    Proc. Natl. Acad. Sci. U.S.A. 96:8901-8906(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.63 ANGSTROMS) OF 53-128 IN COMPLEX WITH CPEA2; CPEB AND 15,16-DIHYDROBILIVERDIN, SUBUNIT, HYDROXYLATION AT LYS-56.
  4. "Developing a structure-function model for the cryptophyte phycoerythrin 545 using ultrahigh resolution crystallography and ultrafast laser spectroscopy."
    Doust A.B., Marai C.N., Harrop S.J., Wilk K.E., Curmi P.M., Scholes G.D.
    J. Mol. Biol. 344:135-153(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (0.97 ANGSTROMS) OF 53-128 IN COMPLEX WITH CPEA2; CPEB AND 15,16-DIHYDROBILIVERDIN, SUBUNIT, HYDROXYLATION AT LYS-56.

Entry informationi

Entry nameiPHE3_RHDS2
AccessioniPrimary (citable) accession number: Q00433
Secondary accession number(s): O82050
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: May 27, 2002
Last modified: October 29, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

The light-harvesting system in Cryptophytes contains phycobiliprotein complexes. Unusually they are composed of either phycoerythrin (CPE) or phycocyanin (CPC) but never allophycocyanin (APC), with only one type of biliprotein being present in any one species. Unlike cyanobacteria or red algae these proteins are not arranged into higher-order phycobilisome complexes, and they are found in the thylakoid lumen.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3