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Q00422 (GABPA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GA-binding protein alpha chain

Short name=GABP subunit alpha
Gene names
Name:Gabpa
Synonyms:E4tf1a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length454 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor capable of interacting with purine rich repeats (GA repeats).

Subunit structure

Heterotetramer of two alpha and two beta subunits.

Subcellular location

Nucleus.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the ETS family.

Contains 1 ETS DNA-binding domain.

Contains 1 PNT (pointed) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processin utero embryonic development

Inferred from mutant phenotype PubMed 17485447. Source: MGI

negative regulation of megakaryocyte differentiation

Inferred from mutant phenotype PubMed 16757682. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 17485447. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence orthology PubMed 9857059. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15361867. Source: MGI

transcription from RNA polymerase II promoter

Inferred from Biological aspect of Ancestor. Source: GOC

   Cellular_componentnuclear chromatin

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 11004506PubMed 15361867. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 11004506PubMed 15361867. Source: MGI

RNA polymerase II core promoter proximal region sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from electronic annotation. Source: Ensembl

chromatin binding

Inferred from direct assay PubMed 16757682. Source: MGI

protein heterodimerization activity

Inferred from sequence orthology PubMed 9857059. Source: MGI

sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

transcription regulatory region DNA binding

Inferred from sequence orthology PubMed 9857059. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 454454GA-binding protein alpha chain
PRO_0000204128

Regions

Domain168 – 25184PNT
DNA binding320 – 40081ETS

Experimental info

Sequence conflict1881H → R in AAA53030. Ref.1

Secondary structure

.................................................. 454
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q00422 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 2CBC40B895563685

FASTA45451,344
        10         20         30         40         50         60 
MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYTPAECVSQ AIDINEPIGN LKKLLEPRLQ 

        70         80         90        100        110        120 
CSLDAHEICL QDIQLDPDRS LFDQGVKTDG TVQLSVQVIS YQGMEPKLNI LEIVKTAETV 

       130        140        150        160        170        180 
EVVIDPDAHH AEAEAHLVEE AQVITLDGTK HITTISDETS EQVTRWAAAL EGYRKEQERL 

       190        200        210        220        230        240 
GIPYDPIHWS TDQVLHWVVW VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL 

       250        260        270        280        290        300 
WSHLELLRKY VLASQEQQMN EIVTIDQPVQ IIPASVPPAT PTTIKVINSS AKAAKVQRSP 

       310        320        330        340        350        360 
RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL NQPELVAQKW 

       370        380        390        400        410        420 
GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV CDLKTLIGYS AAELNRLVIE 

       430        440        450 
CEQKKLARMQ LHGIAQPVTA VALAATSLQA DKEI 

« Hide

References

« Hide 'large scale' references
[1]"Identification of Ets- and notch-related subunits in GA binding protein."
Lamarco K., Thompson C.C., Byers B.P., Walton E.M., McKnight S.L.
Science 253:789-792(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Bone marrow, Egg and Liver.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[5]"The structure of GABPalpha/beta: an ETS domain-ankyrin repeat heterodimer bound to DNA."
Batchelor A.H., Piper D.E., de la Brousse F.C., McKnight S.L., Wolberger C.
Science 279:1037-1041(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 320-320.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M74515 mRNA. Translation: AAA53030.1.
AK139916 mRNA. Translation: BAE24181.1.
AK145446 mRNA. Translation: BAE26442.1.
AK145838 mRNA. Translation: BAE26687.1.
AK148087 mRNA. Translation: BAE28337.1.
AK150455 mRNA. Translation: BAE29575.1.
CH466521 Genomic DNA. Translation: EDK98317.1.
BC052448 mRNA. Translation: AAH52448.1.
CCDSCCDS28284.1.
PIRA40858.
RefSeqNP_032091.2. NM_008065.2.
XP_006522970.1. XM_006522907.1.
UniGeneMm.18974.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AWCX-ray2.15A320-429[»]
1SXDNMR-A168-254[»]
2JUONMR-A35-121[»]
ProteinModelPortalQ00422.
SMRQ00422. Positions 35-121, 168-254, 320-429.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-156N.
IntActQ00422. 1 interaction.
MINTMINT-1531786.

PTM databases

PhosphoSiteQ00422.

Proteomic databases

MaxQBQ00422.
PaxDbQ00422.
PRIDEQ00422.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000009120; ENSMUSP00000009120; ENSMUSG00000008976.
ENSMUST00000114184; ENSMUSP00000109822; ENSMUSG00000008976.
GeneID14390.
KEGGmmu:14390.
UCSCuc007ztj.1. mouse.

Organism-specific databases

CTD2551.
MGIMGI:95610. Gabpa.

Phylogenomic databases

eggNOGNOG252121.
GeneTreeENSGT00750000117307.
HOGENOMHOG000285952.
HOVERGENHBG051687.
InParanoidQ7TT22.
KOK09441.
OMAGIPYDPV.
OrthoDBEOG7TXKGT.
TreeFamTF350537.

Gene expression databases

ArrayExpressQ00422.
BgeeQ00422.
CleanExMM_GABPA.
GenevestigatorQ00422.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProIPR000418. Ets_dom.
IPR024668. GABP_asu_N.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR016312. TF_GA-bd_asu.
IPR029071. Ubiquitin-rel_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00178. Ets. 1 hit.
PF11620. GABP-alpha. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PIRSFPIRSF001703. GABP_alpha. 1 hit.
PRINTSPR00454. ETSDOMAIN.
SMARTSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGABPA. mouse.
EvolutionaryTraceQ00422.
NextBio285913.
PROQ00422.
SOURCESearch...

Entry information

Entry nameGABPA_MOUSE
AccessionPrimary (citable) accession number: Q00422
Secondary accession number(s): Q7TT22
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot