Q00422 (GABPA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GA-binding protein alpha chain
Short name=GABP subunit alpha

Gene names

Name:	Gabpa
Synonyms:	E4tf1a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor capable of interacting with purine rich repeats (GA repeats).
Subunit structure	Heterotetramer of two alpha and two beta subunits.
Subcellular location	Nucleus.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	in utero embryonic development Inferred from mutant phenotype PubMed 17485447. Source: MGI negative regulation of megakaryocyte differentiation Inferred from mutant phenotype PubMed 16757682. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 17485447. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from sequence orthology PubMed 9857059. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay PubMed 11004506PubMed 15361867. Source: MGI
Molecular_function	chromatin binding Inferred from direct assay PubMed 16757682. Source: MGI protein heterodimerization activity Inferred from sequence orthology PubMed 9857059. Source: MGI sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro transcription regulatory region DNA binding Inferred from sequence orthology PubMed 9857059. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 454

454

GA-binding protein alpha chain

PRO_0000204128

Regions

Domain

168 – 251

84

PNT

DNA binding

320 – 400

81

ETS

Experimental info

Sequence conflict

188

1

H → R in AAA53030. Ref.1

Secondary structure

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454

Helix Strand Turn

Details...

Beta strand

36 – 47

12

Helix

48 – 50

3

Helix

51 – 54

4

Helix

56 – 59

4

Beta strand

67 – 70

4

Turn

81 – 83

3

Beta strand

90 – 99

10

Beta strand

107 – 115

9

Helix

168 – 177

10

Turn

178 – 180

3

Helix

186 – 188

3

Helix

191 – 204

14

Helix

213 – 215

3

Helix

219 – 224

6

Helix

227 – 233

7

Helix

238 – 249

12

Turn

250 – 252

3

Helix

322 – 330

9

Turn

333 – 338

6

Beta strand

339 – 341

3

Beta strand

343 – 349

7

Helix

353 – 364

12

Helix

371 – 379

9

Helix

380 – 383

4

Beta strand

384 – 389

6

Beta strand

396 – 399

4

Helix

403 – 406

4

Helix

411 – 428

18

Sequences

	Sequence		Length	Mass (Da)
	Q00422 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 2CBC40B895563685	FASTA	454	51,344
	10 20 30 40 50 60 MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYTPAECVSQ AIDINEPIGN LKKLLEPRLQ 70 80 90 100 110 120 CSLDAHEICL QDIQLDPDRS LFDQGVKTDG TVQLSVQVIS YQGMEPKLNI LEIVKTAETV 130 140 150 160 170 180 EVVIDPDAHH AEAEAHLVEE AQVITLDGTK HITTISDETS EQVTRWAAAL EGYRKEQERL 190 200 210 220 230 240 GIPYDPIHWS TDQVLHWVVW VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL 250 260 270 280 290 300 WSHLELLRKY VLASQEQQMN EIVTIDQPVQ IIPASVPPAT PTTIKVINSS AKAAKVQRSP 310 320 330 340 350 360 RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL NQPELVAQKW 370 380 390 400 410 420 GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV CDLKTLIGYS AAELNRLVIE 430 440 450 CEQKKLARMQ LHGIAQPVTA VALAATSLQA DKEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of Ets- and notch-related subunits in GA binding protein." Lamarco K., Thompson C.C., Byers B.P., Walton E.M., McKnight S.L. Science 253:789-792(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Bone marrow, Egg and Liver.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[5]	"The structure of GABPalpha/beta: an ETS domain-ankyrin repeat heterodimer bound to DNA." Batchelor A.H., Piper D.E., de la Brousse F.C., McKnight S.L., Wolberger C. Science 279:1037-1041(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 320-320.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M74515 mRNA. Translation: AAA53030.1.
AK139916 mRNA. Translation: BAE24181.1.
AK145446 mRNA. Translation: BAE26442.1.
AK145838 mRNA. Translation: BAE26687.1.
AK148087 mRNA. Translation: BAE28337.1.
AK150455 mRNA. Translation: BAE29575.1.
CH466521 Genomic DNA. Translation: EDK98317.1.
BC052448 mRNA. Translation: AAH52448.1.

IPI

IPI00118948.

PIR

A40858.

RefSeq

NP_032091.2. NM_008065.2.

UniGene

Mm.18974.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AWC	X-ray	2.15	A	320-429	[»]
1SXD	NMR	-	A	168-254	[»]
2JUO	NMR	-	A	35-121	[»]

ProteinModelPortal

Q00422.

SMR

Q00422. Positions 35-121, 168-254, 320-429.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-156N.

PTM databases

PhosphoSite

Q00422.

Proteomic databases

PaxDb

Q00422.

PRIDE

Q00422.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000009120; ENSMUSP00000009120; ENSMUSG00000008976.
ENSMUST00000114184; ENSMUSP00000109822; ENSMUSG00000008976.

GeneID

14390.

KEGG

mmu:14390.

Organism-specific databases

CTD

2551.

MGI

MGI:95610. Gabpa.

Phylogenomic databases

eggNOG

NOG252121.

GeneTree

ENSGT00700000104086.

HOGENOM

HOG000285952.

HOVERGEN

HBG051687.

InParanoid

Q7TT22.

KO

K09441.

OMA

GIPYDPV.

OrthoDB

EOG41NTKX.

Gene expression databases

ArrayExpress

Q00422.

Bgee

Q00422.

CleanEx

MM_GABPA.

Genevestigator

Q00422.

GermOnline

ENSMUSG00000008976. Mus musculus.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.
1.10.150.50. 1 hit.

InterPro

IPR000418. Ets_dom.
IPR024668. GABP_asu_N.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR016312. TF_GA-bd_asu.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00178. Ets. 1 hit.
PF11620. GABP-alpha. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]

PIRSF

PIRSF001703. GABP_alpha. 1 hit.

PRINTS

PR00454. ETSDOMAIN.

SMART

SM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]

SUPFAM

SSF47769. SAM_homology. 1 hit.

PROSITE

PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

GABPA. mouse.

EvolutionaryTrace

Q00422.

NextBio

285913.

SOURCE

Search...

Entry information

Entry name

GABPA_MOUSE

Accession

Primary (citable) accession number: Q00422
Secondary accession number(s): Q7TT22

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program