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Protein

GA-binding protein alpha chain

Gene

Gabpa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor capable of interacting with purine rich repeats (GA repeats).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi320 – 400ETSPROSITE-ProRule annotationAdd BLAST81

GO - Molecular functioni

GO - Biological processi

  • in utero embryonic development Source: MGI
  • negative regulation of megakaryocyte differentiation Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-2151201. Transcriptional activation of mitochondrial biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
GA-binding protein alpha chain
Short name:
GABP subunit alpha
Gene namesi
Name:Gabpa
Synonyms:E4tf1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:95610. Gabpa.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromatin Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041281 – 454GA-binding protein alpha chainAdd BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei303PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ00422.
MaxQBiQ00422.
PaxDbiQ00422.
PeptideAtlasiQ00422.
PRIDEiQ00422.

PTM databases

iPTMnetiQ00422.
PhosphoSitePlusiQ00422.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSMUSG00000008976.
CleanExiMM_GABPA.
GenevisibleiQ00422. MM.

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta subunits.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199795. 1 interactor.
DIPiDIP-156N.
IntActiQ00422. 1 interactor.
MINTiMINT-1531786.
STRINGi10090.ENSMUSP00000009120.

Structurei

Secondary structure

1454
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi36 – 47Combined sources12
Helixi48 – 50Combined sources3
Helixi51 – 54Combined sources4
Helixi56 – 59Combined sources4
Beta strandi67 – 70Combined sources4
Turni81 – 83Combined sources3
Beta strandi90 – 99Combined sources10
Beta strandi107 – 115Combined sources9
Helixi168 – 177Combined sources10
Turni178 – 180Combined sources3
Helixi186 – 188Combined sources3
Helixi191 – 204Combined sources14
Helixi213 – 215Combined sources3
Helixi219 – 224Combined sources6
Helixi227 – 233Combined sources7
Helixi238 – 249Combined sources12
Turni250 – 252Combined sources3
Helixi322 – 330Combined sources9
Turni333 – 338Combined sources6
Beta strandi339 – 341Combined sources3
Beta strandi343 – 349Combined sources7
Helixi353 – 364Combined sources12
Helixi371 – 379Combined sources9
Helixi380 – 383Combined sources4
Beta strandi384 – 389Combined sources6
Beta strandi396 – 399Combined sources4
Helixi403 – 406Combined sources4
Helixi411 – 428Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AWCX-ray2.15A320-429[»]
1SXDNMR-A168-254[»]
2JUONMR-A35-121[»]
ProteinModelPortaliQ00422.
SMRiQ00422.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00422.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini168 – 251PNTPROSITE-ProRule annotationAdd BLAST84

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118907.
HOGENOMiHOG000285952.
HOVERGENiHBG051687.
InParanoidiQ00422.
KOiK09441.
OMAiGIPYDPV.
OrthoDBiEOG091G0EXE.
TreeFamiTF350537.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR024668. GABP_asu_N.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR016312. TF_GA-bd_asu.
IPR029071. Ubiquitin-rel_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF11620. GABP-alpha. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PIRSFiPIRSF001703. GABP_alpha. 1 hit.
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYTPAECVSQ AIDINEPIGN
60 70 80 90 100
LKKLLEPRLQ CSLDAHEICL QDIQLDPDRS LFDQGVKTDG TVQLSVQVIS
110 120 130 140 150
YQGMEPKLNI LEIVKTAETV EVVIDPDAHH AEAEAHLVEE AQVITLDGTK
160 170 180 190 200
HITTISDETS EQVTRWAAAL EGYRKEQERL GIPYDPIHWS TDQVLHWVVW
210 220 230 240 250
VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL WSHLELLRKY
260 270 280 290 300
VLASQEQQMN EIVTIDQPVQ IIPASVPPAT PTTIKVINSS AKAAKVQRSP
310 320 330 340 350
RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL
360 370 380 390 400
NQPELVAQKW GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV
410 420 430 440 450
CDLKTLIGYS AAELNRLVIE CEQKKLARMQ LHGIAQPVTA VALAATSLQA

DKEI
Length:454
Mass (Da):51,344
Last modified:July 27, 2011 - v2
Checksum:i2CBC40B895563685
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti188H → R in AAA53030 (PubMed:1876836).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74515 mRNA. Translation: AAA53030.1.
AK139916 mRNA. Translation: BAE24181.1.
AK145446 mRNA. Translation: BAE26442.1.
AK145838 mRNA. Translation: BAE26687.1.
AK148087 mRNA. Translation: BAE28337.1.
AK150455 mRNA. Translation: BAE29575.1.
CH466521 Genomic DNA. Translation: EDK98317.1.
BC052448 mRNA. Translation: AAH52448.1.
CCDSiCCDS28284.1.
PIRiA40858.
RefSeqiNP_032091.2. NM_008065.2.
XP_006522970.1. XM_006522907.2.
UniGeneiMm.18974.

Genome annotation databases

EnsembliENSMUST00000009120; ENSMUSP00000009120; ENSMUSG00000008976.
ENSMUST00000114184; ENSMUSP00000109822; ENSMUSG00000008976.
GeneIDi14390.
KEGGimmu:14390.
UCSCiuc007ztj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74515 mRNA. Translation: AAA53030.1.
AK139916 mRNA. Translation: BAE24181.1.
AK145446 mRNA. Translation: BAE26442.1.
AK145838 mRNA. Translation: BAE26687.1.
AK148087 mRNA. Translation: BAE28337.1.
AK150455 mRNA. Translation: BAE29575.1.
CH466521 Genomic DNA. Translation: EDK98317.1.
BC052448 mRNA. Translation: AAH52448.1.
CCDSiCCDS28284.1.
PIRiA40858.
RefSeqiNP_032091.2. NM_008065.2.
XP_006522970.1. XM_006522907.2.
UniGeneiMm.18974.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AWCX-ray2.15A320-429[»]
1SXDNMR-A168-254[»]
2JUONMR-A35-121[»]
ProteinModelPortaliQ00422.
SMRiQ00422.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199795. 1 interactor.
DIPiDIP-156N.
IntActiQ00422. 1 interactor.
MINTiMINT-1531786.
STRINGi10090.ENSMUSP00000009120.

PTM databases

iPTMnetiQ00422.
PhosphoSitePlusiQ00422.

Proteomic databases

EPDiQ00422.
MaxQBiQ00422.
PaxDbiQ00422.
PeptideAtlasiQ00422.
PRIDEiQ00422.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000009120; ENSMUSP00000009120; ENSMUSG00000008976.
ENSMUST00000114184; ENSMUSP00000109822; ENSMUSG00000008976.
GeneIDi14390.
KEGGimmu:14390.
UCSCiuc007ztj.1. mouse.

Organism-specific databases

CTDi2551.
MGIiMGI:95610. Gabpa.

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118907.
HOGENOMiHOG000285952.
HOVERGENiHBG051687.
InParanoidiQ00422.
KOiK09441.
OMAiGIPYDPV.
OrthoDBiEOG091G0EXE.
TreeFamiTF350537.

Enzyme and pathway databases

ReactomeiR-MMU-2151201. Transcriptional activation of mitochondrial biogenesis.

Miscellaneous databases

EvolutionaryTraceiQ00422.
PROiQ00422.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000008976.
CleanExiMM_GABPA.
GenevisibleiQ00422. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR024668. GABP_asu_N.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR016312. TF_GA-bd_asu.
IPR029071. Ubiquitin-rel_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF11620. GABP-alpha. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PIRSFiPIRSF001703. GABP_alpha. 1 hit.
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGABPA_MOUSE
AccessioniPrimary (citable) accession number: Q00422
Secondary accession number(s): Q7TT22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.