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Q00422

- GABPA_MOUSE

UniProt

Q00422 - GABPA_MOUSE

Protein

GA-binding protein alpha chain

Gene

Gabpa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Transcription factor capable of interacting with purine rich repeats (GA repeats).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi320 – 40081ETSPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: MGI
    3. protein heterodimerization activity Source: MGI
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: Ensembl
    5. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
    6. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome
    7. transcription regulatory region DNA binding Source: MGI

    GO - Biological processi

    1. in utero embryonic development Source: MGI
    2. negative regulation of megakaryocyte differentiation Source: MGI
    3. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    4. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    5. regulation of transcription from RNA polymerase II promoter Source: MGI
    6. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_205251. Transcriptional activation of mitochondrial biogenesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GA-binding protein alpha chain
    Short name:
    GABP subunit alpha
    Gene namesi
    Name:Gabpa
    Synonyms:E4tf1a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:95610. Gabpa.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear chromatin Source: Ensembl
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 454454GA-binding protein alpha chainPRO_0000204128Add
    BLAST

    Proteomic databases

    MaxQBiQ00422.
    PaxDbiQ00422.
    PRIDEiQ00422.

    PTM databases

    PhosphoSiteiQ00422.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Gene expression databases

    ArrayExpressiQ00422.
    BgeeiQ00422.
    CleanExiMM_GABPA.
    GenevestigatoriQ00422.

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha and two beta subunits.

    Protein-protein interaction databases

    DIPiDIP-156N.
    IntActiQ00422. 1 interaction.
    MINTiMINT-1531786.

    Structurei

    Secondary structure

    1
    454
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi36 – 4712
    Helixi48 – 503
    Helixi51 – 544
    Helixi56 – 594
    Beta strandi67 – 704
    Turni81 – 833
    Beta strandi90 – 9910
    Beta strandi107 – 1159
    Helixi168 – 17710
    Turni178 – 1803
    Helixi186 – 1883
    Helixi191 – 20414
    Helixi213 – 2153
    Helixi219 – 2246
    Helixi227 – 2337
    Helixi238 – 24912
    Turni250 – 2523
    Helixi322 – 3309
    Turni333 – 3386
    Beta strandi339 – 3413
    Beta strandi343 – 3497
    Helixi353 – 36412
    Helixi371 – 3799
    Helixi380 – 3834
    Beta strandi384 – 3896
    Beta strandi396 – 3994
    Helixi403 – 4064
    Helixi411 – 42818

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AWCX-ray2.15A320-429[»]
    1SXDNMR-A168-254[»]
    2JUONMR-A35-121[»]
    ProteinModelPortaliQ00422.
    SMRiQ00422. Positions 35-121, 168-254, 320-429.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ00422.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini168 – 25184PNTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ETS family.Curated
    Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG252121.
    GeneTreeiENSGT00750000117307.
    HOGENOMiHOG000285952.
    HOVERGENiHBG051687.
    InParanoidiQ7TT22.
    KOiK09441.
    OMAiGIPYDPV.
    OrthoDBiEOG7TXKGT.
    TreeFamiTF350537.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    1.10.150.50. 1 hit.
    InterProiIPR000418. Ets_dom.
    IPR024668. GABP_asu_N.
    IPR003118. Pointed_dom.
    IPR013761. SAM/pointed.
    IPR016312. TF_GA-bd_asu.
    IPR029071. Ubiquitin-rel_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00178. Ets. 1 hit.
    PF11620. GABP-alpha. 1 hit.
    PF02198. SAM_PNT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001703. GABP_alpha. 1 hit.
    PRINTSiPR00454. ETSDOMAIN.
    SMARTiSM00413. ETS. 1 hit.
    SM00251. SAM_PNT. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    PS51433. PNT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00422-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYTPAECVSQ AIDINEPIGN    50
    LKKLLEPRLQ CSLDAHEICL QDIQLDPDRS LFDQGVKTDG TVQLSVQVIS 100
    YQGMEPKLNI LEIVKTAETV EVVIDPDAHH AEAEAHLVEE AQVITLDGTK 150
    HITTISDETS EQVTRWAAAL EGYRKEQERL GIPYDPIHWS TDQVLHWVVW 200
    VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL WSHLELLRKY 250
    VLASQEQQMN EIVTIDQPVQ IIPASVPPAT PTTIKVINSS AKAAKVQRSP 300
    RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL 350
    NQPELVAQKW GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV 400
    CDLKTLIGYS AAELNRLVIE CEQKKLARMQ LHGIAQPVTA VALAATSLQA 450
    DKEI 454
    Length:454
    Mass (Da):51,344
    Last modified:July 27, 2011 - v2
    Checksum:i2CBC40B895563685
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti188 – 1881H → R in AAA53030. (PubMed:1876836)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74515 mRNA. Translation: AAA53030.1.
    AK139916 mRNA. Translation: BAE24181.1.
    AK145446 mRNA. Translation: BAE26442.1.
    AK145838 mRNA. Translation: BAE26687.1.
    AK148087 mRNA. Translation: BAE28337.1.
    AK150455 mRNA. Translation: BAE29575.1.
    CH466521 Genomic DNA. Translation: EDK98317.1.
    BC052448 mRNA. Translation: AAH52448.1.
    CCDSiCCDS28284.1.
    PIRiA40858.
    RefSeqiNP_032091.2. NM_008065.2.
    XP_006522970.1. XM_006522907.1.
    UniGeneiMm.18974.

    Genome annotation databases

    EnsembliENSMUST00000009120; ENSMUSP00000009120; ENSMUSG00000008976.
    ENSMUST00000114184; ENSMUSP00000109822; ENSMUSG00000008976.
    GeneIDi14390.
    KEGGimmu:14390.
    UCSCiuc007ztj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74515 mRNA. Translation: AAA53030.1 .
    AK139916 mRNA. Translation: BAE24181.1 .
    AK145446 mRNA. Translation: BAE26442.1 .
    AK145838 mRNA. Translation: BAE26687.1 .
    AK148087 mRNA. Translation: BAE28337.1 .
    AK150455 mRNA. Translation: BAE29575.1 .
    CH466521 Genomic DNA. Translation: EDK98317.1 .
    BC052448 mRNA. Translation: AAH52448.1 .
    CCDSi CCDS28284.1.
    PIRi A40858.
    RefSeqi NP_032091.2. NM_008065.2.
    XP_006522970.1. XM_006522907.1.
    UniGenei Mm.18974.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AWC X-ray 2.15 A 320-429 [» ]
    1SXD NMR - A 168-254 [» ]
    2JUO NMR - A 35-121 [» ]
    ProteinModelPortali Q00422.
    SMRi Q00422. Positions 35-121, 168-254, 320-429.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-156N.
    IntActi Q00422. 1 interaction.
    MINTi MINT-1531786.

    PTM databases

    PhosphoSitei Q00422.

    Proteomic databases

    MaxQBi Q00422.
    PaxDbi Q00422.
    PRIDEi Q00422.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000009120 ; ENSMUSP00000009120 ; ENSMUSG00000008976 .
    ENSMUST00000114184 ; ENSMUSP00000109822 ; ENSMUSG00000008976 .
    GeneIDi 14390.
    KEGGi mmu:14390.
    UCSCi uc007ztj.1. mouse.

    Organism-specific databases

    CTDi 2551.
    MGIi MGI:95610. Gabpa.

    Phylogenomic databases

    eggNOGi NOG252121.
    GeneTreei ENSGT00750000117307.
    HOGENOMi HOG000285952.
    HOVERGENi HBG051687.
    InParanoidi Q7TT22.
    KOi K09441.
    OMAi GIPYDPV.
    OrthoDBi EOG7TXKGT.
    TreeFami TF350537.

    Enzyme and pathway databases

    Reactomei REACT_205251. Transcriptional activation of mitochondrial biogenesis.

    Miscellaneous databases

    ChiTaRSi GABPA. mouse.
    EvolutionaryTracei Q00422.
    NextBioi 285913.
    PROi Q00422.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q00422.
    Bgeei Q00422.
    CleanExi MM_GABPA.
    Genevestigatori Q00422.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    1.10.150.50. 1 hit.
    InterProi IPR000418. Ets_dom.
    IPR024668. GABP_asu_N.
    IPR003118. Pointed_dom.
    IPR013761. SAM/pointed.
    IPR016312. TF_GA-bd_asu.
    IPR029071. Ubiquitin-rel_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00178. Ets. 1 hit.
    PF11620. GABP-alpha. 1 hit.
    PF02198. SAM_PNT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001703. GABP_alpha. 1 hit.
    PRINTSi PR00454. ETSDOMAIN.
    SMARTi SM00413. ETS. 1 hit.
    SM00251. SAM_PNT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    PS51433. PNT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of Ets- and notch-related subunits in GA binding protein."
      Lamarco K., Thompson C.C., Byers B.P., Walton E.M., McKnight S.L.
      Science 253:789-792(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Bone marrow, Egg and Liver.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    5. "The structure of GABPalpha/beta: an ETS domain-ankyrin repeat heterodimer bound to DNA."
      Batchelor A.H., Piper D.E., de la Brousse F.C., McKnight S.L., Wolberger C.
      Science 279:1037-1041(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 320-320.

    Entry informationi

    Entry nameiGABPA_MOUSE
    AccessioniPrimary (citable) accession number: Q00422
    Secondary accession number(s): Q7TT22
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 138 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3