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Q00417

- TCF7_MOUSE

UniProt

Q00417 - TCF7_MOUSE

Protein

Transcription factor 7

Gene

Tcf7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (25 Mar 2003)
      Previous versions | rss
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    Functioni

    Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ CD8+ immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1 By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi304 – 37269HMG boxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. beta-catenin binding Source: RefGenome
    2. chromatin binding Source: RefGenome
    3. DNA binding Source: MGI
    4. sequence-specific DNA binding Source: RefGenome
    5. sequence-specific DNA binding transcription factor activity Source: MGI
    6. transcription regulatory region DNA binding Source: RefGenome

    GO - Biological processi

    1. alpha-beta T cell differentiation Source: MGI
    2. brain development Source: RefGenome
    3. canonical Wnt signaling pathway involved in negative regulation of apoptotic process Source: MGI
    4. cellular response to interleukin-4 Source: Ensembl
    5. embryonic digestive tract morphogenesis Source: MGI
    6. embryonic genitalia morphogenesis Source: MGI
    7. embryonic hindgut morphogenesis Source: MGI
    8. generation of neurons Source: RefGenome
    9. neural tube development Source: MGI
    10. regulation of cell proliferation Source: MGI
    11. regulation of transcription, DNA-templated Source: MGI
    12. regulation of transcription from RNA polymerase II promoter Source: RefGenome
    13. T cell receptor V(D)J recombination Source: MGI
    14. transcription, DNA-templated Source: UniProtKB-KW
    15. Wnt signaling pathway Source: MGI

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation, Wnt signaling pathway

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor 7
    Short name:
    TCF-7
    Alternative name(s):
    T-cell-specific transcription factor 1
    Short name:
    T-cell factor 1
    Short name:
    TCF-1
    Gene namesi
    Name:Tcf7
    Synonyms:Tcf-1, Tcf1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:98507. Tcf7.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB
    2. transcription factor complex Source: RefGenome

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Tcf7 may allow the formation of epithelial tumors.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 419419Transcription factor 7PRO_0000048613Add
    BLAST

    Proteomic databases

    MaxQBiQ00417.
    PRIDEiQ00417.

    PTM databases

    PhosphoSiteiQ00417.

    Expressioni

    Tissue specificityi

    T-cell specific.

    Gene expression databases

    ArrayExpressiQ00417.
    BgeeiQ00417.
    CleanExiMM_TCF7.
    GenevestigatoriQ00417.

    Interactioni

    Subunit structurei

    Binds the armadillo repeat of CTNNB1 and forms a stable complex. Binds AES, TLE1, TLE2, TLE3 and TLE4 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ00417. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00417.
    SMRiQ00417. Positions 2-59, 304-378.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 6060CTNNB1-bindingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi379 – 3857Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi7 – 148Poly-Gly

    Sequence similaritiesi

    Belongs to the TCF/LEF family.Curated
    Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG252916.
    HOGENOMiHOG000116032.
    HOVERGENiHBG000419.
    InParanoidiQ00417.
    KOiK02620.
    OMAiHISADIN.
    OrthoDBiEOG7QNVMG.
    PhylomeDBiQ00417.
    TreeFamiTF318448.

    Family and domain databases

    Gene3Di1.10.30.10. 1 hit.
    4.10.900.10. 1 hit.
    InterProiIPR027397. Catenin_binding_dom.
    IPR013558. CTNNB1-bd_N.
    IPR009071. HMG_box_dom.
    IPR024940. TCF/LEF.
    IPR028785. Tcf7.
    [Graphical view]
    PANTHERiPTHR10373. PTHR10373. 1 hit.
    PTHR10373:SF33. PTHR10373:SF33. 1 hit.
    PfamiPF08347. CTNNB1_binding. 1 hit.
    PF00505. HMG_box. 1 hit.
    [Graphical view]
    SMARTiSM00398. HMG. 1 hit.
    [Graphical view]
    SUPFAMiSSF47095. SSF47095. 1 hit.
    PROSITEiPS50118. HMG_BOX_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative promoter usage. Align

    Note: Additional isoforms seem to exist.

    Isoform 2 (identifier: Q00417-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPQLDSGGGG AGRGDDLGAP DELLAFQDEG EEQDDKNRDS PVGPERDLAE    50
    LKSSLVNESE GAAAGAGVPG PGVRVHGEAE GAPEALGREH TSQRLFPDKL 100
    PESLEDGLKA PECTSGMYKE TVYSAFNLLM PYPPASGAGQ HPQPQPPLHN 150
    KPGQPPHGVP QLSPLYEHFS SPHPTPAPAD ISQKQGVHRP LQTPDLSGFY 200
    SLTSGSMGQL PHTVSWPSPP LYPLSPSCGY RQHFPAPTAA PGAPYPRFTH 250
    PSLMLGSGVP GHPAAIPHPA IVPSSGKQEL QPYDRNLKTQ AEPKAEKEAK 300
    KPVIKKPLNA FMLYMKEMRA KVIAECTLKE SAAINQILGR RWHALSREEQ 350
    AKYYELARKE RQLHMQLYPG WSARDNYGKK KRRSREKHQE STTGGKRNAF 400
    GTYPEKAAAP APFLPMTVL 419
    Length:419
    Mass (Da):45,465
    Last modified:March 25, 2003 - v2
    Checksum:iC49C39AE8871C2C0
    GO
    Isoform 1 (identifier: Q00417-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-116: Missing.

    Show »
    Length:303
    Mass (Da):33,609
    Checksum:i14CC7C3E326EAC92
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 116116Missing in isoform 1. 1 PublicationVSP_014963Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61385 mRNA. Translation: CAA43658.1.
    CCDSiCCDS24670.1. [Q00417-2]
    PIRiJH0401.
    RefSeqiNP_033357.1. NM_009331.3. [Q00417-2]
    UniGeneiMm.31630.

    Genome annotation databases

    EnsembliENSMUST00000086844; ENSMUSP00000084055; ENSMUSG00000000782. [Q00417-2]
    ENSMUST00000109071; ENSMUSP00000104699; ENSMUSG00000000782. [Q00417-2]
    GeneIDi21414.
    KEGGimmu:21414.
    UCSCiuc007ivj.2. mouse. [Q00417-1]

    Keywords - Coding sequence diversityi

    Alternative promoter usage

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61385 mRNA. Translation: CAA43658.1 .
    CCDSi CCDS24670.1. [Q00417-2 ]
    PIRi JH0401.
    RefSeqi NP_033357.1. NM_009331.3. [Q00417-2 ]
    UniGenei Mm.31630.

    3D structure databases

    ProteinModelPortali Q00417.
    SMRi Q00417. Positions 2-59, 304-378.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q00417. 1 interaction.

    PTM databases

    PhosphoSitei Q00417.

    Proteomic databases

    MaxQBi Q00417.
    PRIDEi Q00417.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000086844 ; ENSMUSP00000084055 ; ENSMUSG00000000782 . [Q00417-2 ]
    ENSMUST00000109071 ; ENSMUSP00000104699 ; ENSMUSG00000000782 . [Q00417-2 ]
    GeneIDi 21414.
    KEGGi mmu:21414.
    UCSCi uc007ivj.2. mouse. [Q00417-1 ]

    Organism-specific databases

    CTDi 6932.
    MGIi MGI:98507. Tcf7.

    Phylogenomic databases

    eggNOGi NOG252916.
    HOGENOMi HOG000116032.
    HOVERGENi HBG000419.
    InParanoidi Q00417.
    KOi K02620.
    OMAi HISADIN.
    OrthoDBi EOG7QNVMG.
    PhylomeDBi Q00417.
    TreeFami TF318448.

    Miscellaneous databases

    ChiTaRSi TCF7. mouse.
    NextBioi 300706.
    PROi Q00417.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q00417.
    Bgeei Q00417.
    CleanExi MM_TCF7.
    Genevestigatori Q00417.

    Family and domain databases

    Gene3Di 1.10.30.10. 1 hit.
    4.10.900.10. 1 hit.
    InterProi IPR027397. Catenin_binding_dom.
    IPR013558. CTNNB1-bd_N.
    IPR009071. HMG_box_dom.
    IPR024940. TCF/LEF.
    IPR028785. Tcf7.
    [Graphical view ]
    PANTHERi PTHR10373. PTHR10373. 1 hit.
    PTHR10373:SF33. PTHR10373:SF33. 1 hit.
    Pfami PF08347. CTNNB1_binding. 1 hit.
    PF00505. HMG_box. 1 hit.
    [Graphical view ]
    SMARTi SM00398. HMG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47095. SSF47095. 1 hit.
    PROSITEi PS50118. HMG_BOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of murine TCF-1, a T cell-specific transcription factor interacting with functional motifs in the CD3-epsilon and T cell receptor alpha enhancers."
      Oosterwegel M.A., Clevers H.
      J. Exp. Med. 173:1133-1142(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Thymus.
    2. "Extensive alternative splicing and dual promoter usage generate Tcf-1 protein isoforms with differential transcription control properties."
      Van de Wetering M., Castrop J., Korinek V., Clevers H.
      Mol. Cell. Biol. 16:745-752(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: UTILIZATION OF AN UPSTREAM PROMOTER, IDENTIFICATION OF ISOFORM 2.
    3. "Synergy between tumor suppressor APC and the beta-catenin-Tcf4 target Tcf1."
      Roose J., Huls G., van Beest M., Moerer P., van der Horn K., Goldschmeding R., Logtenberg T., Clevers H.
      Science 285:1923-1926(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISEASE.
    4. "The beta-catenin-TCF-1 pathway ensures CD4(+)CD8(+) thymocyte survival."
      Ioannidis V., Beermann F., Clevers H., Held W.
      Nat. Immunol. 2:691-697(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiTCF7_MOUSE
    AccessioniPrimary (citable) accession number: Q00417
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: March 25, 2003
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3