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Protein

Transcription factor 7

Gene

Tcf7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ CD8+ immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1 (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi304 – 372HMG boxPROSITE-ProRule annotationAdd BLAST69

GO - Molecular functioni

  • beta-catenin binding Source: GO_Central
  • DNA binding Source: MGI
  • DNA binding transcription factor activity Source: MGI
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: BHF-UCL
  • sequence-specific DNA binding Source: GO_Central
  • transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: BHF-UCL

GO - Biological processi

  • alpha-beta T cell differentiation Source: MGI
  • canonical Wnt signaling pathway involved in negative regulation of apoptotic process Source: MGI
  • cellular response to interleukin-4 Source: Ensembl
  • embryonic digestive tract morphogenesis Source: MGI
  • embryonic genitalia morphogenesis Source: MGI
  • embryonic hindgut morphogenesis Source: MGI
  • negative regulation of transcription by RNA polymerase II Source: BHF-UCL
  • neural tube development Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • T cell receptor V(D)J recombination Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • Wnt signaling pathway Source: MGI

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-201722 Formation of the beta-catenin:TCF transactivating complex
R-MMU-3769402 Deactivation of the beta-catenin transactivating complex
R-MMU-4641265 Repression of WNT target genes
R-MMU-8951430 RUNX3 regulates WNT signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 7
Short name:
TCF-7
Alternative name(s):
T-cell-specific transcription factor 1
Short name:
T-cell factor 1
Short name:
TCF-1
Gene namesi
Name:Tcf7
Synonyms:Tcf-1, Tcf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98507 Tcf7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Tcf7 may allow the formation of epithelial tumors.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000486131 – 419Transcription factor 7Add BLAST419

Proteomic databases

MaxQBiQ00417
PaxDbiQ00417
PeptideAtlasiQ00417
PRIDEiQ00417

PTM databases

PhosphoSitePlusiQ00417

Expressioni

Tissue specificityi

T-cell specific.

Gene expression databases

BgeeiENSMUSG00000000782
CleanExiMM_TCF7
ExpressionAtlasiQ00417 baseline and differential
GenevisibleiQ00417 MM

Interactioni

Subunit structurei

Binds the armadillo repeat of CTNNB1 and forms a stable complex. Binds AES, TLE1, TLE2, TLE3 and TLE4. Interacts with MLLT11.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204007, 1 interactor
IntActiQ00417, 1 interactor
STRINGi10090.ENSMUSP00000084055

Structurei

3D structure databases

ProteinModelPortaliQ00417
SMRiQ00417
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 60CTNNB1-bindingBy similarityAdd BLAST60

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi379 – 385Nuclear localization signalSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 14Poly-Gly8

Sequence similaritiesi

Belongs to the TCF/LEF family.Curated

Phylogenomic databases

eggNOGiKOG3248 Eukaryota
ENOG41109RU LUCA
GeneTreeiENSGT00390000009964
HOGENOMiHOG000116032
HOVERGENiHBG000419
InParanoidiQ00417
KOiK02620
OMAiECTSGMY
PhylomeDBiQ00417
TreeFamiTF318448

Family and domain databases

Gene3Di1.10.30.10, 1 hit
4.10.900.10, 1 hit
InterProiView protein in InterPro
IPR027397 Catenin_binding_dom_sf
IPR013558 CTNNB1-bd_N
IPR009071 HMG_box_dom
IPR036910 HMG_box_dom_sf
IPR024940 TCF/LEF
IPR028785 Tcf7
PANTHERiPTHR10373 PTHR10373, 1 hit
PTHR10373:SF33 PTHR10373:SF33, 1 hit
PfamiView protein in Pfam
PF08347 CTNNB1_binding, 1 hit
PF00505 HMG_box, 1 hit
SMARTiView protein in SMART
SM00398 HMG, 1 hit
SUPFAMiSSF47095 SSF47095, 1 hit
PROSITEiView protein in PROSITE
PS50118 HMG_BOX_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 2 (identifier: Q00417-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPQLDSGGGG AGRGDDLGAP DELLAFQDEG EEQDDKNRDS PVGPERDLAE
60 70 80 90 100
LKSSLVNESE GAAAGAGVPG PGVRVHGEAE GAPEALGREH TSQRLFPDKL
110 120 130 140 150
PESLEDGLKA PECTSGMYKE TVYSAFNLLM PYPPASGAGQ HPQPQPPLHN
160 170 180 190 200
KPGQPPHGVP QLSPLYEHFS SPHPTPAPAD ISQKQGVHRP LQTPDLSGFY
210 220 230 240 250
SLTSGSMGQL PHTVSWPSPP LYPLSPSCGY RQHFPAPTAA PGAPYPRFTH
260 270 280 290 300
PSLMLGSGVP GHPAAIPHPA IVPSSGKQEL QPYDRNLKTQ AEPKAEKEAK
310 320 330 340 350
KPVIKKPLNA FMLYMKEMRA KVIAECTLKE SAAINQILGR RWHALSREEQ
360 370 380 390 400
AKYYELARKE RQLHMQLYPG WSARDNYGKK KRRSREKHQE STTGGKRNAF
410
GTYPEKAAAP APFLPMTVL
Length:419
Mass (Da):45,465
Last modified:March 25, 2003 - v2
Checksum:iC49C39AE8871C2C0
GO
Isoform 1 (identifier: Q00417-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.

Show »
Length:303
Mass (Da):33,609
Checksum:i14CC7C3E326EAC92
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0149631 – 116Missing in isoform 1. 1 PublicationAdd BLAST116

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61385 mRNA Translation: CAA43658.1
CCDSiCCDS24670.1 [Q00417-2]
PIRiJH0401
RefSeqiNP_001300910.1, NM_001313981.1 [Q00417-2]
NP_033357.1, NM_009331.4 [Q00417-2]
UniGeneiMm.31630

Genome annotation databases

EnsembliENSMUST00000086844; ENSMUSP00000084055; ENSMUSG00000000782 [Q00417-2]
ENSMUST00000109071; ENSMUSP00000104699; ENSMUSG00000000782 [Q00417-2]
GeneIDi21414
KEGGimmu:21414
UCSCiuc007ivj.2 mouse [Q00417-1]

Keywords - Coding sequence diversityi

Alternative promoter usage

Similar proteinsi

Entry informationi

Entry nameiTCF7_MOUSE
AccessioniPrimary (citable) accession number: Q00417
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: March 25, 2003
Last modified: April 25, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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