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Q00417 (TCF7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor 7

Short name=TCF-7
Alternative name(s):
T-cell-specific transcription factor 1
Short name=T-cell factor 1
Short name=TCF-1
Gene names
Name:Tcf7
Synonyms:Tcf-1, Tcf1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ CD8+ immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1 By similarity. Ref.4

Subunit structure

Binds the armadillo repeat of CTNNB1 and forms a stable complex. Binds AES, TLE1, TLE2, TLE3 and TLE4 By similarity.

Subcellular location

Nucleus.

Tissue specificity

T-cell specific.

Involvement in disease

Defects in Tcf7 may allow the formation of epithelial tumors. Ref.3

Sequence similarities

Belongs to the TCF/LEF family.

Contains 1 HMG box DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
Wnt signaling pathway
   Cellular componentNucleus
   Coding sequence diversityAlternative promoter usage
   LigandDNA-binding
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT cell receptor V(D)J recombination

Inferred from genetic interaction PubMed 9462507. Source: MGI

Wnt signaling pathway

Inferred from direct assay PubMed 12235125. Source: MGI

alpha-beta T cell differentiation

Inferred from genetic interaction PubMed 9462507. Source: MGI

brain development

Inferred from Biological aspect of Ancestor. Source: RefGenome

canonical Wnt signaling pathway involved in negative regulation of apoptotic process

Inferred from direct assay PubMed 12235125. Source: MGI

cellular response to interleukin-4

Inferred from electronic annotation. Source: Ensembl

embryonic digestive tract morphogenesis

Inferred from genetic interaction PubMed 15057272. Source: MGI

embryonic genitalia morphogenesis

Inferred from genetic interaction PubMed 15057272. Source: MGI

embryonic hindgut morphogenesis

Inferred from genetic interaction PubMed 15057272. Source: MGI

generation of neurons

Inferred from Biological aspect of Ancestor. Source: RefGenome

neural tube development

Inferred from genetic interaction PubMed 15057272. Source: MGI

regulation of cell proliferation

Inferred from direct assay PubMed 12235125. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of transcription, DNA-templated

Inferred from direct assay PubMed 12235125. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay PubMed 18579517. Source: UniProtKB

transcription factor complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionDNA binding

Inferred from direct assay PubMed 12235125Ref.1. Source: MGI

beta-catenin binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

chromatin binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

sequence-specific DNA binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 12235125. Source: MGI

transcription regulatory region DNA binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 2 (identifier: Q00417-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: Q00417-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 419419Transcription factor 7
PRO_0000048613

Regions

DNA binding304 – 37269HMG box
Region1 – 6060CTNNB1-binding By similarity
Motif379 – 3857Nuclear localization signal Potential
Compositional bias7 – 148Poly-Gly

Natural variations

Alternative sequence1 – 116116Missing in isoform 1.
VSP_014963

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 [UniParc].

Last modified March 25, 2003. Version 2.
Checksum: C49C39AE8871C2C0

FASTA41945,465
        10         20         30         40         50         60 
MPQLDSGGGG AGRGDDLGAP DELLAFQDEG EEQDDKNRDS PVGPERDLAE LKSSLVNESE 

        70         80         90        100        110        120 
GAAAGAGVPG PGVRVHGEAE GAPEALGREH TSQRLFPDKL PESLEDGLKA PECTSGMYKE 

       130        140        150        160        170        180 
TVYSAFNLLM PYPPASGAGQ HPQPQPPLHN KPGQPPHGVP QLSPLYEHFS SPHPTPAPAD 

       190        200        210        220        230        240 
ISQKQGVHRP LQTPDLSGFY SLTSGSMGQL PHTVSWPSPP LYPLSPSCGY RQHFPAPTAA 

       250        260        270        280        290        300 
PGAPYPRFTH PSLMLGSGVP GHPAAIPHPA IVPSSGKQEL QPYDRNLKTQ AEPKAEKEAK 

       310        320        330        340        350        360 
KPVIKKPLNA FMLYMKEMRA KVIAECTLKE SAAINQILGR RWHALSREEQ AKYYELARKE 

       370        380        390        400        410 
RQLHMQLYPG WSARDNYGKK KRRSREKHQE STTGGKRNAF GTYPEKAAAP APFLPMTVL 

« Hide

Isoform 1 [UniParc].

Checksum: 14CC7C3E326EAC92
Show »

FASTA30333,609

References

[1]"Cloning of murine TCF-1, a T cell-specific transcription factor interacting with functional motifs in the CD3-epsilon and T cell receptor alpha enhancers."
Oosterwegel M.A., Clevers H.
J. Exp. Med. 173:1133-1142(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Thymus.
[2]"Extensive alternative splicing and dual promoter usage generate Tcf-1 protein isoforms with differential transcription control properties."
Van de Wetering M., Castrop J., Korinek V., Clevers H.
Mol. Cell. Biol. 16:745-752(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: UTILIZATION OF AN UPSTREAM PROMOTER, IDENTIFICATION OF ISOFORM 2.
[3]"Synergy between tumor suppressor APC and the beta-catenin-Tcf4 target Tcf1."
Roose J., Huls G., van Beest M., Moerer P., van der Horn K., Goldschmeding R., Logtenberg T., Clevers H.
Science 285:1923-1926(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: DISEASE.
[4]"The beta-catenin-TCF-1 pathway ensures CD4(+)CD8(+) thymocyte survival."
Ioannidis V., Beermann F., Clevers H., Held W.
Nat. Immunol. 2:691-697(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61385 mRNA. Translation: CAA43658.1.
CCDSCCDS24670.1. [Q00417-2]
PIRJH0401.
RefSeqNP_033357.1. NM_009331.3. [Q00417-2]
UniGeneMm.31630.

3D structure databases

ProteinModelPortalQ00417.
SMRQ00417. Positions 2-59, 304-378.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ00417. 1 interaction.

PTM databases

PhosphoSiteQ00417.

Proteomic databases

MaxQBQ00417.
PRIDEQ00417.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000086844; ENSMUSP00000084055; ENSMUSG00000000782. [Q00417-2]
ENSMUST00000109071; ENSMUSP00000104699; ENSMUSG00000000782. [Q00417-2]
GeneID21414.
KEGGmmu:21414.
UCSCuc007ivj.2. mouse. [Q00417-1]

Organism-specific databases

CTD6932.
MGIMGI:98507. Tcf7.

Phylogenomic databases

eggNOGNOG252916.
HOGENOMHOG000116032.
HOVERGENHBG000419.
InParanoidQ00417.
KOK02620.
OMAHISADIN.
OrthoDBEOG7QNVMG.
PhylomeDBQ00417.
TreeFamTF318448.

Gene expression databases

ArrayExpressQ00417.
BgeeQ00417.
CleanExMM_TCF7.
GenevestigatorQ00417.

Family and domain databases

Gene3D1.10.30.10. 1 hit.
4.10.900.10. 1 hit.
InterProIPR027397. Catenin_binding_dom.
IPR013558. CTNNB1-bd_N.
IPR009071. HMG_box_dom.
IPR024940. TCF/LEF.
IPR028785. Tcf7.
[Graphical view]
PANTHERPTHR10373. PTHR10373. 1 hit.
PTHR10373:SF33. PTHR10373:SF33. 1 hit.
PfamPF08347. CTNNB1_binding. 1 hit.
PF00505. HMG_box. 1 hit.
[Graphical view]
SMARTSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMSSF47095. SSF47095. 1 hit.
PROSITEPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTCF7. mouse.
NextBio300706.
PROQ00417.
SOURCESearch...

Entry information

Entry nameTCF7_MOUSE
AccessionPrimary (citable) accession number: Q00417
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: March 25, 2003
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot