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Q00417

- TCF7_MOUSE

UniProt

Q00417 - TCF7_MOUSE

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Protein
Transcription factor 7
Gene
Tcf7, Tcf-1, Tcf1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ CD8+ immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1 By similarity.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi304 – 37269HMG box
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. beta-catenin binding Source: RefGenome
  3. chromatin binding Source: RefGenome
  4. sequence-specific DNA binding Source: RefGenome
  5. sequence-specific DNA binding transcription factor activity Source: MGI
  6. transcription regulatory region DNA binding Source: RefGenome
Complete GO annotation...

GO - Biological processi

  1. T cell receptor V(D)J recombination Source: MGI
  2. Wnt signaling pathway Source: MGI
  3. alpha-beta T cell differentiation Source: MGI
  4. brain development Source: RefGenome
  5. canonical Wnt signaling pathway involved in negative regulation of apoptotic process Source: MGI
  6. cellular response to interleukin-4 Source: Ensembl
  7. embryonic digestive tract morphogenesis Source: MGI
  8. embryonic genitalia morphogenesis Source: MGI
  9. embryonic hindgut morphogenesis Source: MGI
  10. generation of neurons Source: RefGenome
  11. neural tube development Source: MGI
  12. regulation of cell proliferation Source: MGI
  13. regulation of transcription from RNA polymerase II promoter Source: RefGenome
  14. regulation of transcription, DNA-templated Source: MGI
  15. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Wnt signaling pathway

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 7
Short name:
TCF-7
Alternative name(s):
T-cell-specific transcription factor 1
Short name:
T-cell factor 1
Short name:
TCF-1
Gene namesi
Name:Tcf7
Synonyms:Tcf-1, Tcf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:98507. Tcf7.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB
  2. transcription factor complex Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Tcf7 may allow the formation of epithelial tumors.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419Transcription factor 7
PRO_0000048613Add
BLAST

Proteomic databases

MaxQBiQ00417.
PRIDEiQ00417.

PTM databases

PhosphoSiteiQ00417.

Expressioni

Tissue specificityi

T-cell specific.

Gene expression databases

ArrayExpressiQ00417.
BgeeiQ00417.
CleanExiMM_TCF7.
GenevestigatoriQ00417.

Interactioni

Subunit structurei

Binds the armadillo repeat of CTNNB1 and forms a stable complex. Binds AES, TLE1, TLE2, TLE3 and TLE4 By similarity.

Protein-protein interaction databases

IntActiQ00417. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ00417.
SMRiQ00417. Positions 2-59, 304-378.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 6060CTNNB1-binding By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi379 – 3857Nuclear localization signal Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 148Poly-Gly

Sequence similaritiesi

Belongs to the TCF/LEF family.

Phylogenomic databases

eggNOGiNOG252916.
HOGENOMiHOG000116032.
HOVERGENiHBG000419.
InParanoidiQ00417.
KOiK02620.
OMAiHISADIN.
OrthoDBiEOG7QNVMG.
PhylomeDBiQ00417.
TreeFamiTF318448.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
4.10.900.10. 1 hit.
InterProiIPR027397. Catenin_binding_dom.
IPR013558. CTNNB1-bd_N.
IPR009071. HMG_box_dom.
IPR024940. TCF/LEF.
IPR028785. Tcf7.
[Graphical view]
PANTHERiPTHR10373. PTHR10373. 1 hit.
PTHR10373:SF33. PTHR10373:SF33. 1 hit.
PfamiPF08347. CTNNB1_binding. 1 hit.
PF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. Align

Note: Additional isoforms seem to exist.

Isoform 2 (identifier: Q00417-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPQLDSGGGG AGRGDDLGAP DELLAFQDEG EEQDDKNRDS PVGPERDLAE    50
LKSSLVNESE GAAAGAGVPG PGVRVHGEAE GAPEALGREH TSQRLFPDKL 100
PESLEDGLKA PECTSGMYKE TVYSAFNLLM PYPPASGAGQ HPQPQPPLHN 150
KPGQPPHGVP QLSPLYEHFS SPHPTPAPAD ISQKQGVHRP LQTPDLSGFY 200
SLTSGSMGQL PHTVSWPSPP LYPLSPSCGY RQHFPAPTAA PGAPYPRFTH 250
PSLMLGSGVP GHPAAIPHPA IVPSSGKQEL QPYDRNLKTQ AEPKAEKEAK 300
KPVIKKPLNA FMLYMKEMRA KVIAECTLKE SAAINQILGR RWHALSREEQ 350
AKYYELARKE RQLHMQLYPG WSARDNYGKK KRRSREKHQE STTGGKRNAF 400
GTYPEKAAAP APFLPMTVL 419
Length:419
Mass (Da):45,465
Last modified:March 25, 2003 - v2
Checksum:iC49C39AE8871C2C0
GO
Isoform 1 (identifier: Q00417-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: Missing.

Show »
Length:303
Mass (Da):33,609
Checksum:i14CC7C3E326EAC92
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 116116Missing in isoform 1.
VSP_014963Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61385 mRNA. Translation: CAA43658.1.
CCDSiCCDS24670.1. [Q00417-2]
PIRiJH0401.
RefSeqiNP_033357.1. NM_009331.3. [Q00417-2]
UniGeneiMm.31630.

Genome annotation databases

EnsembliENSMUST00000086844; ENSMUSP00000084055; ENSMUSG00000000782. [Q00417-2]
ENSMUST00000109071; ENSMUSP00000104699; ENSMUSG00000000782. [Q00417-2]
GeneIDi21414.
KEGGimmu:21414.
UCSCiuc007ivj.2. mouse. [Q00417-1]

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61385 mRNA. Translation: CAA43658.1 .
CCDSi CCDS24670.1. [Q00417-2 ]
PIRi JH0401.
RefSeqi NP_033357.1. NM_009331.3. [Q00417-2 ]
UniGenei Mm.31630.

3D structure databases

ProteinModelPortali Q00417.
SMRi Q00417. Positions 2-59, 304-378.
ModBasei Search...

Protein-protein interaction databases

IntActi Q00417. 1 interaction.

PTM databases

PhosphoSitei Q00417.

Proteomic databases

MaxQBi Q00417.
PRIDEi Q00417.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000086844 ; ENSMUSP00000084055 ; ENSMUSG00000000782 . [Q00417-2 ]
ENSMUST00000109071 ; ENSMUSP00000104699 ; ENSMUSG00000000782 . [Q00417-2 ]
GeneIDi 21414.
KEGGi mmu:21414.
UCSCi uc007ivj.2. mouse. [Q00417-1 ]

Organism-specific databases

CTDi 6932.
MGIi MGI:98507. Tcf7.

Phylogenomic databases

eggNOGi NOG252916.
HOGENOMi HOG000116032.
HOVERGENi HBG000419.
InParanoidi Q00417.
KOi K02620.
OMAi HISADIN.
OrthoDBi EOG7QNVMG.
PhylomeDBi Q00417.
TreeFami TF318448.

Miscellaneous databases

ChiTaRSi TCF7. mouse.
NextBioi 300706.
PROi Q00417.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q00417.
Bgeei Q00417.
CleanExi MM_TCF7.
Genevestigatori Q00417.

Family and domain databases

Gene3Di 1.10.30.10. 1 hit.
4.10.900.10. 1 hit.
InterProi IPR027397. Catenin_binding_dom.
IPR013558. CTNNB1-bd_N.
IPR009071. HMG_box_dom.
IPR024940. TCF/LEF.
IPR028785. Tcf7.
[Graphical view ]
PANTHERi PTHR10373. PTHR10373. 1 hit.
PTHR10373:SF33. PTHR10373:SF33. 1 hit.
Pfami PF08347. CTNNB1_binding. 1 hit.
PF00505. HMG_box. 1 hit.
[Graphical view ]
SMARTi SM00398. HMG. 1 hit.
[Graphical view ]
SUPFAMi SSF47095. SSF47095. 1 hit.
PROSITEi PS50118. HMG_BOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of murine TCF-1, a T cell-specific transcription factor interacting with functional motifs in the CD3-epsilon and T cell receptor alpha enhancers."
    Oosterwegel M.A., Clevers H.
    J. Exp. Med. 173:1133-1142(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Thymus.
  2. "Extensive alternative splicing and dual promoter usage generate Tcf-1 protein isoforms with differential transcription control properties."
    Van de Wetering M., Castrop J., Korinek V., Clevers H.
    Mol. Cell. Biol. 16:745-752(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: UTILIZATION OF AN UPSTREAM PROMOTER, IDENTIFICATION OF ISOFORM 2.
  3. "Synergy between tumor suppressor APC and the beta-catenin-Tcf4 target Tcf1."
    Roose J., Huls G., van Beest M., Moerer P., van der Horn K., Goldschmeding R., Logtenberg T., Clevers H.
    Science 285:1923-1926(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISEASE.
  4. "The beta-catenin-TCF-1 pathway ensures CD4(+)CD8(+) thymocyte survival."
    Ioannidis V., Beermann F., Clevers H., Held W.
    Nat. Immunol. 2:691-697(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTCF7_MOUSE
AccessioniPrimary (citable) accession number: Q00417
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: March 25, 2003
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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