Reviewed, UniProtKB/Swiss-Prot Q00415 (HPPD_COCP7)

Names and origin

Protein names

Recommended name:
    4-hydroxyphenylpyruvate dioxygenase
      Short name=HPPDase
      Short name=4HPPD
      Short name=HPD
    EC=1.13.11.27
Alternative name(s):
    T-cell reactive protein

Gene names

Name:	TCRP
ORF Names:	CPC735_051010

Organism

Coccidioides posadasii (strain C735) (Valley fever fungus)

Taxonomic identifier

222929 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	4-hydroxyphenylpyruvate + O2 = homogentisate + CO2.
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.
Sequence similarities	Belongs to the 4HPPD family.
Sequence caution	The sequence BAE20309.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAE20310.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAE20311.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAE20312.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAE20313.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAE20314.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAE20315.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAE20318.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAE20321.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAE20322.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAE20324.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAE20325.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAE20326.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAE20327.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-hydroxyphenylpyruvate dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	4-hydroxyphenylpyruvate dioxygenase		PRO_0000088403
Sites
	Metal binding	200	1	Iron By similarity
	Metal binding	283	1	Iron By similarity
	Metal binding	366	1	Iron By similarity
Natural variations
	Natural variant	250	1	N → S in strain: IFM 45817. Ref.5
Experimental info
	Sequence conflict	297	1	N → T in AAC60567. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q00415-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 342DEFF33091C3C2	FASTA	399	45,262
	10 20 30 40 50 60 MAPAADSPTL QPAQPSDLNQ YRGYDHVHWY VGNAKQAATY YVTRMGFERV AYRGLETGSK 70 80 90 100 110 120 AVASHVVRNG NITFILTSPL RSVEQASRFP EDEALLKEIH AHLERHGDGV KDVAFEVDCV 130 140 150 160 170 180 ESVFSAAVRN GAEVVSDVRT VEDEDGQIKM ATIRTYGETT HTLIERSGYR GGFMPGYRME 190 200 210 220 230 240 SNADATSKFL PKVVLERIDH CVGNQDWDEM ERVCDYYEKI LGFHRFWSVD DKDICTEFSA 250 260 270 280 290 300 LKSIVMASPN DIVKMPINEP AKGKKQSQIE EYVDFYNGAG VQHIALRTNN IIDAITNLKA 310 320 330 340 350 360 RGTEFIKVPE TYYEDMKIRL KRQGLVLDED FETLKSLDIL IDFDENGYLL QLFTKHLMDR 370 380 390 PTVFIEIIQR NNFSGFGAGN FRALFEAIER EQALRGTLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a gene encoding a T-cell reactive protein from Coccidioides immitis: homology to 4-hydroxyphenylpyruvate dioxygenase and the mammalian F antigen." Wyckoff E.E., Pishko E.J., Kirkland T.N., Cole G.T. Gene 161:107-111(1995) [PubMed: 7642122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed: 19717792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Concordance of gene genealogies reveals reproductive isolation in the pathogenic fungus Coccidioides immitis." Koufopanou V., Burt A., Taylor J.W. Proc. Natl. Acad. Sci. U.S.A. 94:5478-5482(1997) [PubMed: 9144263] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 233-362. Strain: RMSCC 757 / Silveira.
[4]	Erratum Koufopanou V., Burt A., Taylor J.W. Proc. Natl. Acad. Sci. U.S.A. 95:8414-8414(1998)
[5]	"Reexamination of Coccidioides spp. reserved in the Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, based on a multiple gene analysis." Sano A., Miyaji M., Kamei K., Mikami Y., Nishimura K. Nippon Ishinkin Gakkai Zasshi 47:113-117(2006) [PubMed: 16699492] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 237-383, VARIANT SER-250. Strain: IFM 45809, IFM 45810, IFM 45811, IFM 45812, IFM 45813, IFM 45817, IFM 4935, IFM 4945, IFM 50993, IFM 50994, IFM 51112, IFM 54194, IFM 54195 and IFM 54196.
[6]	"Coccidioides immitis fractions which are antigenic for immune T lymphocytes." Kirkland T.N., Zhu S., Kruse D., Hsu L., Seshan K.R., Cole G.T. Infect. Immun. 59:3952-3961(1991) [PubMed: 1840578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 237-305.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L38493 Genomic DNA. Translation: AAA82574.1. ACFW01000049 Genomic DNA. Translation: EER23731.1. AJ291968 Genomic DNA. Translation: CAC35132.1. AB232864 Genomic DNA. Translation: BAE20309.1. Sequence problems. AB232865 Genomic DNA. Translation: BAE20310.1. Sequence problems. AB232866 Genomic DNA. Translation: BAE20311.1. Sequence problems. AB232867 Genomic DNA. Translation: BAE20312.1. Sequence problems. AB232868 Genomic DNA. Translation: BAE20313.1. Sequence problems. AB232869 Genomic DNA. Translation: BAE20314.1. Sequence problems. AB232870 Genomic DNA. Translation: BAE20315.1. Sequence problems. AB232873 Genomic DNA. Translation: BAE20318.1. Sequence problems. AB232876 Genomic DNA. Translation: BAE20321.1. Sequence problems. AB232877 Genomic DNA. Translation: BAE20322.1. Sequence problems. AB232879 Genomic DNA. Translation: BAE20324.1. Sequence problems. AB232880 Genomic DNA. Translation: BAE20325.1. Sequence problems. AB232881 Genomic DNA. Translation: BAE20326.1. Sequence problems. AB232882 Genomic DNA. Translation: BAE20327.1. Sequence problems. S69110 mRNA. Translation: AAC60567.2.
3D structure databases
SMR	Q00415. Positions 17-383.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.11.27. 281080.
Family and domain databases
InterPro	IPR005956. 4OHPhenylPyrv_dOase. IPR004360. Glyas_bleo-R_dOase. [Graphical view]
PANTHER	PTHR11959. HPP_dOase. 1 hit.
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
PIRSF	PIRSF009283. HPP_dOase. 1 hit.
TIGRFAMs	TIGR01263. 4HPPD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPD_COCP7

Accession

Primary (citable) accession number: Q00415
Secondary accession number(s): C5PGS8 , Q02288, Q400Q0, Q400Q8, Q9C0M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)