Reviewed, UniProtKB/Swiss-Prot Q00410 (VHUU_METVO)

Names and origin

Protein names

Recommended name:
    F420-non-reducing hydrogenase vhu subunit U
    EC=1.12.99.-

Gene names

Name:

vhuU

Organism

Methanococcus voltae

Taxonomic identifier

2188 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	44 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Cofactor	Nickel Potential.
Subunit structure	The F420-non-reducing hydrogenase vhu is composed of four subunits; vhuA, vhuD, vhuG and vhuU By similarity.
Miscellaneous	The large subunit of vhu is split into vhuA and vhuU. Each contributes two ligands to the [NiFeSe] center. The peptide is so short that about 65% of the chains are released from the ribosome before the initiator Met can be removed.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Coding sequence diversity	Selenocysteine
Ligand	Metal-binding Nickel Selenium
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed; partial
	Chain	2 – 26	25	F420-non-reducing hydrogenase vhu subunit U		PRO_0000013411
	Propeptide	27 – 44	18	Removed in mature form		PRO_0000013412
Sites
	Metal binding	20	1	Nickel Potential
	Metal binding	23	1	Nickel Potential
Amino acid modifications
	Non-standard residue	20	1	Selenocysteine Probable

Sequences

	Sequence		Length	Mass (Da)
	Q00410-1 [UniParc]. Last modified February 26, 2008. Version 6. Checksum: AE001A84B7F714B0	FASTA	44	5,020
	10 20 30 40 MVDETKLNLI EIVLRAYDPU YSCAAHMIVE DAEGNVVFEI VNDE

References

[1]	"Methanococcus voltae harbors four gene clusters potentially encoding two [NiFe] and two [NiFeSe] hydrogenases, each of the cofactor F420-reducing or F420-non-reducing types." Halboth S., Klein A. Mol. Gen. Genet. 233:217-224(1992) [PubMed: 1603063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 1537 / PS.
[2]	"A novel very small subunit of a selenium containing [NiFe] hydrogenase of Methanococcus voltae is postranslationally processed by cleavage at a defined position." Sorgenfrei O., Linder D., Karas M., Klein A. Eur. J. Biochem. 213:1355-1358(1993) [PubMed: 8504827] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-26, PROTEOLYTIC PROCESSING.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X61204 Genomic DNA. Translation: CAA43511.1.
PIR	S32463.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
PROSITE	PS00508. NI_HGENASE_L_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VHUU_METVO

Accession

Primary (citable) accession number: Q00410
Secondary accession number(s): P95319, Q7M534

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	February 26, 2008
Last modified:	May 5, 2009
This is version 48 of the entry and version 6 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)