Reviewed, UniProtKB/Swiss-Prot Q00407 (VHUA_METVO)

Names and origin

Protein names

Recommended name:
    F420-non-reducing hydrogenase vhu subunit A
    EC=1.12.99.-

Gene names

Name:

vhuA

Organism

Methanococcus voltae

Taxonomic identifier

2188 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Cofactor	Nickel Potential.
Subunit structure	The F420-non-reducing hydrogenase vhu is composed of four subunits; vhuA, vhuD, vhuG and vhuU By similarity.
Miscellaneous	The large subunit of vhu is split into vhuA and vhuU. Each contributes two ligands to the [NiFeSe] center.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	F420-non-reducing hydrogenase vhu subunit A		PRO_0000199731
Sites
	Metal binding	61	1	Nickel Potential
	Metal binding	64	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	Q00407-1 [UniParc]. Last modified May 1, 1997. Version 2. Checksum: 6C27359F703DFC96	FASTA	420	46,066
	10 20 30 40 50 60 MGKITIAPLT RLEGHGKVTI KLDDSGKPAD VKLHITALRG FEQFVIGRPA EEVPRIVPRI 70 80 90 100 110 120 CGICQTAHHL ASVKAVDAAW GAQIPSAAEK QRELMHLGNM IHSHALHFYY LAAPDFVLGP 130 140 150 160 170 180 DADPAIRNIV GVIDAAPEVA KKAIAMRRVG QSMVEATGGK PIHPVTGIPG GLSKSMSEEK 190 200 210 220 230 240 RDELLAEIDT MIQYGQDGLD LMKSLNEKYL DTINSLGVID TWYLGLVKDG KHNFYGDTLR 250 260 270 280 290 300 FVSPDGSEKM EFKPAEYLDY LGEHVVEHSY VKYPYNKKVG YPEGLYRVGP LAMINVCDSM 310 320 330 340 350 360 STPLAEEARK EFAETFGRPA NQSIAYNQAR LIELLSACER AKELLEDPEI VSTDVKAEVE 370 380 390 400 410 420 PKAGNGVGVV YAPRGTLFHN YETDDNGIVT KANMIVATTH NVPTMEKAIQ QAAEVLFKDN

References

[1]

"Methanococcus voltae harbors four gene clusters potentially encoding two [NiFe] and two [NiFeSe] hydrogenases, each of the cofactor F420-reducing or F420-non-reducing types." Halboth S., Klein A. Mol. Gen. Genet. 233:217-224(1992) [PubMed: 1603063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 1537 / PS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X61204 Genomic DNA. Translation: CAA43510.1.
PIR	A59304.
3D structure databases
HSSP	HSSP built from PDB template 1CC1 based on UniProtKB P13065.
ModBase	Search...
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VHUA_METVO

Accession

Primary (citable) accession number: Q00407

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	May 1, 1997
Last modified:	June 16, 2009
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)