Reviewed, UniProtKB/Swiss-Prot Q00404 (VHCA_METVO)

Names and origin

Protein names

Recommended name:
    F420-non-reducing hydrogenase vhc subunit A
    EC=1.12.99.-

Gene names

Name:

vhcA

Organism

Methanococcus voltae

Taxonomic identifier

2188 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Cofactor	Nickel Potential.
Subunit structure	The F420-non-reducing hydrogenase vhc is composed of three subunits; vhcA, vhcD and vhcG By similarity.
Induction	By selenium deprivation.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	F420-non-reducing hydrogenase vhc subunit A		PRO_0000199729
Sites
	Metal binding	61	1	Nickel Potential
	Metal binding	64	1	Nickel Potential
	Metal binding	445	1	Nickel Potential
	Metal binding	448	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	Q00404-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4BA3F16EC5F18B44	FASTA	474	52,914
	10 20 30 40 50 60 MTKLSIEPVT RVEGHGKVTL SFDDSGKLDK VNFHVVEVRG FEKFLEGRYI EDAPIFTPRI 70 80 90 100 110 120 CGICQVSHHL ASAKAVDNVF GVKIPETANM LRNLIHQASN VHSHALHFGM LASPDLMFPT 130 140 150 160 170 180 TDDALKRNLL GVAGENMDMI KDSIAMRKVG QTIVQKVGGR AIHPVTAIVG GQSKPLTEEE 190 200 210 220 230 240 RDELLTLSDN LVETAERTLN VGKEMVENLK EKDLMNLGYF ESYHMGLVNN GAQDIYEGNI 250 260 270 280 290 300 RVVNSEGKIE YEFDPAEYAN YITEGVRPYS YLKFLALLKR RGEGKGAYRV NTLSRLNVCD 310 320 330 340 350 360 KMPTPIAQKY YEEFVKTYGK PAHQPMLFHY ARLIELLSSS ELIRKFLEDD KIVDTDVRAE 370 380 390 400 410 420 VDPKNITGEG VGCVEAPRGT LIHHFKTDDR GIINDTNLVV ATVQNNPAMD IGVQKVAEKY 430 440 450 460 470 IKTPEVAKPH ILNHMEMVIR AYDPCLSCAT HTIGEEPKIL SIHVCQGGKL IKTL

References

[1]	"Methanococcus voltae harbors four gene clusters potentially encoding two [NiFe] and two [NiFeSe] hydrogenases, each of the cofactor F420-reducing or F420-non-reducing types." Halboth S., Klein A. Mol. Gen. Genet. 233:217-224(1992) [PubMed: 1603063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 1537 / PS.
[2]	"Selenium is involved in the negative regulation of the expression of selenium-free [NiFe] hydrogenases in Methanococcus voltae." Berghoefer Y., Agha-Amiri K., Klein A. Mol. Gen. Genet. 242:369-373(1994) [PubMed: 8121392] [Abstract] Cited for: TRANSCRIPTIONAL REGULATION. Strain: DSM 1537 / PS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X61203 Genomic DNA. Translation: CAA43506.1.
PIR	S16727.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 2 hits. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VHCA_METVO

Accession

Primary (citable) accession number: Q00404

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)