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Protein

Transcription initiation factor IIB

Gene

GTF2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi15Zinc1
Metal bindingi18Zinc1
Metal bindingi34Zinc1
Metal bindingi37Zinc1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 42TFIIB-typePROSITE-ProRule annotationAdd BLAST32

GO - Molecular functioni

  • core promoter binding Source: Ensembl
  • thyroid hormone receptor binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000137947-MONOMER.
ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6807505. RNA polymerase II transcribes snRNA genes.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
SIGNORiQ00403.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIB
Alternative name(s):
General transcription factor TFIIB
S300-II
Gene namesi
Name:GTF2B
Synonyms:TF2B, TFIIB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:4648. GTF2B.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: HPA
  • nucleoplasm Source: Reactome
  • nucleus Source: HPA
  • transcription factor TFIID complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51 – 56EWRTFS → AWRTFA: Partial loss of HIV-1 Vpr binding. 6
Mutagenesisi52W → A: Partial loss of HIV-1 Vpr binding. 1
Mutagenesisi53 – 54RT → AA: Partial loss of HIV-1 Vpr binding. 2
Mutagenesisi55F → A: Partial loss of HIV-1 Vpr binding. 1

Organism-specific databases

DisGeNETi2959.
OpenTargetsiENSG00000137947.
PharmGKBiPA29035.

Polymorphism and mutation databases

BioMutaiGTF2B.
DMDMi135629.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192931 – 316Transcription initiation factor IIBAdd BLAST316

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70PhosphoserineCombined sources1
Modified residuei76PhosphoserineCombined sources1
Modified residuei92PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ00403.
MaxQBiQ00403.
PaxDbiQ00403.
PeptideAtlasiQ00403.
PRIDEiQ00403.

PTM databases

iPTMnetiQ00403.
PhosphoSitePlusiQ00403.

Miscellaneous databases

PMAP-CutDBQ00403.

Expressioni

Gene expression databases

BgeeiENSG00000137947.
CleanExiHS_GTF2B.
ExpressionAtlasiQ00403. baseline and differential.
GenevisibleiQ00403. HS.

Organism-specific databases

HPAiHPA061626.

Interactioni

Subunit structurei

Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2. Interacts with HIV-1 Vpr. Interacts with GPBP1 (By similarity). Interacts with Epstein-Barr virus EBNA2. Interacts with herpes simplex virus 1 protein ICP4.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DRAP1Q149195EBI-389564,EBI-712941
EP300Q094722EBI-389564,EBI-447295
GRF4P460772EBI-389564,EBI-637479From a different organism.
ICP0P876623EBI-389564,EBI-11712595From a different organism.
IEP174734EBI-389564,EBI-11702772From a different organism.
TBPP202262EBI-389564,EBI-355371
TNIP1Q150255EBI-389564,EBI-357849
VdrP482812EBI-389564,EBI-346797From a different organism.

GO - Molecular functioni

  • thyroid hormone receptor binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi109214. 88 interactors.
DIPiDIP-1077N.
IntActiQ00403. 33 interactors.
MINTiMINT-156550.
STRINGi9606.ENSP00000359531.

Structurei

Secondary structure

1316
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Beta strandi18 – 21Combined sources4
Beta strandi24 – 26Combined sources3
Beta strandi27 – 29Combined sources3
Turni35 – 37Combined sources3
Helixi111 – 113Combined sources3
Helixi114 – 127Combined sources14
Helixi132 – 148Combined sources17
Beta strandi152 – 154Combined sources3
Helixi156 – 170Combined sources15
Helixi177 – 182Combined sources6
Beta strandi184 – 186Combined sources3
Helixi188 – 201Combined sources14
Helixi212 – 221Combined sources10
Helixi226 – 241Combined sources16
Helixi250 – 263Combined sources14
Beta strandi265 – 267Combined sources3
Helixi271 – 278Combined sources8
Helixi282 – 292Combined sources11
Helixi293 – 295Combined sources3
Helixi296 – 299Combined sources4
Helixi310 – 312Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C9BX-ray2.65A/E/I/M/Q110-316[»]
1DL6NMR-A2-59[»]
1RLYNMR-A1-60[»]
1RO4NMR-A1-60[»]
1TFBNMR-A112-316[»]
1VOLX-ray2.70A113-316[»]
2PHGNMR-A112-316[»]
5IY6electron microscopy7.20M1-316[»]
5IY7electron microscopy8.60M1-316[»]
5IY8electron microscopy7.90M1-316[»]
5IY9electron microscopy6.30M1-316[»]
5IYAelectron microscopy5.40M1-316[»]
5IYBelectron microscopy3.90M1-316[»]
5IYCelectron microscopy3.90M1-316[»]
5IYDelectron microscopy3.90M1-316[»]
ProteinModelPortaliQ00403.
SMRiQ00403.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00403.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati124 – 2001Add BLAST77
Repeati218 – 2942Add BLAST77

Sequence similaritiesi

Belongs to the TFIIB family.Curated
Contains 1 TFIIB-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 42TFIIB-typePROSITE-ProRule annotationAdd BLAST32

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1597. Eukaryota.
COG1405. LUCA.
GeneTreeiENSGT00390000006671.
HOGENOMiHOG000108634.
HOVERGENiHBG017914.
InParanoidiQ00403.
KOiK03124.
OMAiKYVPRFS.
OrthoDBiEOG091G0E7S.
PhylomeDBiQ00403.
TreeFamiTF105953.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00403-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS
60 70 80 90 100
EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK
110 120 130 140 150
YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS
160 170 180 190 200
LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK
210 220 230 240 250
ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP
260 270 280 290 300
ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF
310
PTDFKFDTPV DKLPQL
Length:316
Mass (Da):34,833
Last modified:December 1, 1992 - v1
Checksum:i9CC7E102526C2722
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01197719P → S.Corresponds to variant rs1804499dbSNPEnsembl.1
Natural variantiVAR_035722132R → Q in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant rs144944840dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59268 mRNA. Translation: CAA41958.1.
M76766 mRNA. Translation: AAA61149.1.
AK289822 mRNA. Translation: BAF82511.1.
AB451296 mRNA. Translation: BAG70110.1.
AL445991 Genomic DNA. Translation: CAI41341.1.
BC020597 mRNA. Translation: AAH20597.1.
S44184 mRNA. Translation: AAB23144.1.
CCDSiCCDS715.1.
PIRiS17654. TWHU2B.
RefSeqiNP_001505.1. NM_001514.5.
UniGeneiHs.481852.

Genome annotation databases

EnsembliENST00000370500; ENSP00000359531; ENSG00000137947.
GeneIDi2959.
KEGGihsa:2959.
UCSCiuc001dmo.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59268 mRNA. Translation: CAA41958.1.
M76766 mRNA. Translation: AAA61149.1.
AK289822 mRNA. Translation: BAF82511.1.
AB451296 mRNA. Translation: BAG70110.1.
AL445991 Genomic DNA. Translation: CAI41341.1.
BC020597 mRNA. Translation: AAH20597.1.
S44184 mRNA. Translation: AAB23144.1.
CCDSiCCDS715.1.
PIRiS17654. TWHU2B.
RefSeqiNP_001505.1. NM_001514.5.
UniGeneiHs.481852.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C9BX-ray2.65A/E/I/M/Q110-316[»]
1DL6NMR-A2-59[»]
1RLYNMR-A1-60[»]
1RO4NMR-A1-60[»]
1TFBNMR-A112-316[»]
1VOLX-ray2.70A113-316[»]
2PHGNMR-A112-316[»]
5IY6electron microscopy7.20M1-316[»]
5IY7electron microscopy8.60M1-316[»]
5IY8electron microscopy7.90M1-316[»]
5IY9electron microscopy6.30M1-316[»]
5IYAelectron microscopy5.40M1-316[»]
5IYBelectron microscopy3.90M1-316[»]
5IYCelectron microscopy3.90M1-316[»]
5IYDelectron microscopy3.90M1-316[»]
ProteinModelPortaliQ00403.
SMRiQ00403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109214. 88 interactors.
DIPiDIP-1077N.
IntActiQ00403. 33 interactors.
MINTiMINT-156550.
STRINGi9606.ENSP00000359531.

PTM databases

iPTMnetiQ00403.
PhosphoSitePlusiQ00403.

Polymorphism and mutation databases

BioMutaiGTF2B.
DMDMi135629.

Proteomic databases

EPDiQ00403.
MaxQBiQ00403.
PaxDbiQ00403.
PeptideAtlasiQ00403.
PRIDEiQ00403.

Protocols and materials databases

DNASUi2959.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370500; ENSP00000359531; ENSG00000137947.
GeneIDi2959.
KEGGihsa:2959.
UCSCiuc001dmo.5. human.

Organism-specific databases

CTDi2959.
DisGeNETi2959.
GeneCardsiGTF2B.
HGNCiHGNC:4648. GTF2B.
HPAiHPA061626.
MIMi189963. gene.
neXtProtiNX_Q00403.
OpenTargetsiENSG00000137947.
PharmGKBiPA29035.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1597. Eukaryota.
COG1405. LUCA.
GeneTreeiENSGT00390000006671.
HOGENOMiHOG000108634.
HOVERGENiHBG017914.
InParanoidiQ00403.
KOiK03124.
OMAiKYVPRFS.
OrthoDBiEOG091G0E7S.
PhylomeDBiQ00403.
TreeFamiTF105953.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000137947-MONOMER.
ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6807505. RNA polymerase II transcribes snRNA genes.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
SIGNORiQ00403.

Miscellaneous databases

ChiTaRSiGTF2B. human.
EvolutionaryTraceiQ00403.
GeneWikiiTranscription_factor_II_B.
GenomeRNAii2959.
PMAP-CutDBQ00403.
PROiQ00403.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137947.
CleanExiHS_GTF2B.
ExpressionAtlasiQ00403. baseline and differential.
GenevisibleiQ00403. HS.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTF2B_HUMAN
AccessioniPrimary (citable) accession number: Q00403
Secondary accession number(s): A8K1A7, Q5JS30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 30, 2016
This is version 186 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.