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Protein

Transcription initiation factor IIB

Gene

GTF2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi15Zinc1
Metal bindingi18Zinc1
Metal bindingi34Zinc1
Metal bindingi37Zinc1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 42TFIIB-typePROSITE-ProRule annotationAdd BLAST32

GO - Molecular functioni

  • acetyltransferase activity Source: UniProtKB
  • core promoter binding Source: UniProtKB
  • promoter-specific chromatin binding Source: UniProtKB
  • RNA polymerase II core binding Source: UniProtKB
  • RNA polymerase II core promoter sequence-specific DNA binding Source: UniProtKB
  • TBP-class protein binding Source: CAFA
  • thyroid hormone receptor binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • positive regulation by host of viral transcription Source: UniProtKB
  • positive regulation of core promoter binding Source: CAFA
  • positive regulation of transcription initiation from RNA polymerase II promoter Source: UniProtKB
  • positive regulation of viral transcription Source: AgBase
  • protein acetylation Source: UniProtKB
  • RNA polymerase II core complex assembly Source: UniProtKB
  • RNA polymerase II transcriptional preinitiation complex assembly Source: UniProtKB
  • snRNA transcription by RNA polymerase II Source: Reactome
  • transcriptional start site selection at RNA polymerase II promoter Source: UniProtKB
  • transcription by RNA polymerase II Source: UniProtKB
  • transcription initiation from RNA polymerase II promoter Source: UniProtKB
  • viral process Source: UniProtKB-KW

Keywordsi

Biological processHost-virus interaction, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
SIGNORiQ00403

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIB
Alternative name(s):
General transcription factor TFIIB
S300-II
Gene namesi
Name:GTF2B
Synonyms:TF2B, TFIIB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000137947.11
HGNCiHGNC:4648 GTF2B
MIMi189963 gene
neXtProtiNX_Q00403

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51 – 56EWRTFS → AWRTFA: Partial loss of HIV-1 Vpr binding. 6
Mutagenesisi52W → A: Partial loss of HIV-1 Vpr binding. 1
Mutagenesisi53 – 54RT → AA: Partial loss of HIV-1 Vpr binding. 2
Mutagenesisi55F → A: Partial loss of HIV-1 Vpr binding. 1

Organism-specific databases

DisGeNETi2959
OpenTargetsiENSG00000137947
PharmGKBiPA29035

Polymorphism and mutation databases

BioMutaiGTF2B
DMDMi135629

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192931 – 316Transcription initiation factor IIBAdd BLAST316

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70PhosphoserineCombined sources1
Modified residuei76PhosphoserineCombined sources1
Modified residuei92PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ00403
MaxQBiQ00403
PaxDbiQ00403
PeptideAtlasiQ00403
PRIDEiQ00403
ProteomicsDBi57846

PTM databases

iPTMnetiQ00403
PhosphoSitePlusiQ00403

Miscellaneous databases

PMAP-CutDBiQ00403

Expressioni

Gene expression databases

BgeeiENSG00000137947
CleanExiHS_GTF2B
ExpressionAtlasiQ00403 baseline and differential
GenevisibleiQ00403 HS

Organism-specific databases

HPAiHPA061626

Interactioni

Subunit structurei

Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2 (PubMed:8566795). Interacts with GPBP1 (By similarity). Interacts with HSF1 (via transactivation domain) (PubMed:11005381).By similarity2 Publications
(Microbial infection) Interacts with HIV-1 Vpr.1 Publication
(Microbial infection) Interacts with Epstein-Barr virus EBNA2.1 Publication
(Microbial infection) Interacts with Herpes simplex virus 1 protein ICP4.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • RNA polymerase II core binding Source: UniProtKB
  • TBP-class protein binding Source: CAFA
  • thyroid hormone receptor binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi109214, 90 interactors
CORUMiQ00403
DIPiDIP-1077N
IntActiQ00403, 37 interactors
MINTiQ00403
STRINGi9606.ENSP00000359531

Structurei

Secondary structure

1316
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Beta strandi18 – 21Combined sources4
Beta strandi24 – 26Combined sources3
Beta strandi27 – 29Combined sources3
Turni35 – 37Combined sources3
Helixi111 – 113Combined sources3
Helixi114 – 127Combined sources14
Helixi132 – 148Combined sources17
Helixi149 – 151Combined sources3
Beta strandi152 – 154Combined sources3
Helixi156 – 170Combined sources15
Helixi177 – 182Combined sources6
Beta strandi184 – 186Combined sources3
Helixi188 – 201Combined sources14
Helixi212 – 221Combined sources10
Helixi226 – 241Combined sources16
Helixi250 – 263Combined sources14
Beta strandi265 – 267Combined sources3
Helixi271 – 278Combined sources8
Helixi282 – 292Combined sources11
Helixi293 – 295Combined sources3
Helixi296 – 299Combined sources4
Helixi310 – 312Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C9BX-ray2.65A/E/I/M/Q110-316[»]
1DL6NMR-A2-59[»]
1RLYNMR-A1-60[»]
1RO4NMR-A1-60[»]
1TFBNMR-A112-316[»]
1VOLX-ray2.70A113-316[»]
2PHGNMR-A112-316[»]
5IY6electron microscopy7.20M1-316[»]
5IY7electron microscopy8.60M1-316[»]
5IY8electron microscopy7.90M1-316[»]
5IY9electron microscopy6.30M1-316[»]
5IYAelectron microscopy5.40M1-316[»]
5IYBelectron microscopy3.90M1-316[»]
5IYCelectron microscopy3.90M1-316[»]
5IYDelectron microscopy3.90M1-316[»]
5WH1X-ray3.39A/B/C/D107-316[»]
ProteinModelPortaliQ00403
SMRiQ00403
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00403

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati124 – 2001Add BLAST77
Repeati218 – 2942Add BLAST77

Sequence similaritiesi

Belongs to the TFIIB family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 42TFIIB-typePROSITE-ProRule annotationAdd BLAST32

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1597 Eukaryota
COG1405 LUCA
GeneTreeiENSGT00390000006671
HOGENOMiHOG000108634
HOVERGENiHBG017914
InParanoidiQ00403
KOiK03124
OMAiNDDQGND
OrthoDBiEOG091G0E7S
PhylomeDBiQ00403
TreeFamiTF105953

Family and domain databases

CDDicd00043 CYCLIN, 2 hits
InterProiView protein in InterPro
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR000812 TFIIB
IPR023486 TFIIB_CS
IPR013150 TFIIB_cyclin
IPR013137 Znf_TFIIB
PANTHERiPTHR11618 PTHR11618, 1 hit
PfamiView protein in Pfam
PF08271 TF_Zn_Ribbon, 1 hit
PF00382 TFIIB, 2 hits
PRINTSiPR00685 TIFACTORIIB
SMARTiView protein in SMART
SM00385 CYCLIN, 2 hits
SUPFAMiSSF47954 SSF47954, 2 hits
PROSITEiView protein in PROSITE
PS00782 TFIIB, 2 hits
PS51134 ZF_TFIIB, 1 hit

Sequencei

Sequence statusi: Complete.

Q00403-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS
60 70 80 90 100
EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK
110 120 130 140 150
YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS
160 170 180 190 200
LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK
210 220 230 240 250
ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP
260 270 280 290 300
ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF
310
PTDFKFDTPV DKLPQL
Length:316
Mass (Da):34,833
Last modified:December 1, 1992 - v1
Checksum:i9CC7E102526C2722
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01197719P → S. Corresponds to variant dbSNP:rs1804499Ensembl.1
Natural variantiVAR_035722132R → Q in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs144944840Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59268 mRNA Translation: CAA41958.1
M76766 mRNA Translation: AAA61149.1
AK289822 mRNA Translation: BAF82511.1
AB451296 mRNA Translation: BAG70110.1
AL445991 Genomic DNA No translation available.
BC020597 mRNA Translation: AAH20597.1
S44184 mRNA Translation: AAB23144.1
CCDSiCCDS715.1
PIRiS17654 TWHU2B
RefSeqiNP_001505.1, NM_001514.5
UniGeneiHs.481852

Genome annotation databases

EnsembliENST00000370500; ENSP00000359531; ENSG00000137947
GeneIDi2959
KEGGihsa:2959
UCSCiuc001dmo.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTF2B_HUMAN
AccessioniPrimary (citable) accession number: Q00403
Secondary accession number(s): A8K1A7, Q5JS30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 20, 2018
This is version 200 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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