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Q00403 (TF2B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 160. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription initiation factor IIB
Alternative name(s):
General transcription factor TFIIB
S300-II
Gene names
Name:GTF2B
Synonyms:TF2B, TFIIB
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

Cofactor

Binds 1 zinc ion per subunit.

Subunit structure

Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2. Interacts with HIV-1 Vpr. Interacts with GPBP1 By similarity. Interacts with Epstein-Barr virus EBNA2. Ref.8 Ref.9 Ref.10

Subcellular location

Nucleus.

Sequence similarities

Belongs to the TFIIB family.

Contains 1 TFIIB-type zinc finger.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

EP300Q094722EBI-389564,EBI-447295
GRF4P460772EBI-389564,EBI-637479From a different organism.
VdrP482812EBI-389564,EBI-346797From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 316316Transcription initiation factor IIB
PRO_0000119293

Regions

Repeat124 – 200771
Repeat218 – 294772
Zinc finger11 – 4232TFIIB-type

Sites

Metal binding151Zinc
Metal binding181Zinc
Metal binding341Zinc
Metal binding371Zinc

Amino acid modifications

Modified residue761Phosphoserine Ref.12 Ref.13

Natural variations

Natural variant191P → S.
Corresponds to variant rs1804499 [ dbSNP | Ensembl ].
VAR_011977
Natural variant1321R → Q in a colorectal cancer sample; somatic mutation. Ref.18
VAR_035722

Experimental info

Mutagenesis51 – 566EWRTFS → AWRTFA: Partial loss of HIV-1 Vpr binding.
Mutagenesis521W → A: Partial loss of HIV-1 Vpr binding.
Mutagenesis53 – 542RT → AA: Partial loss of HIV-1 Vpr binding.
Mutagenesis551F → A: Partial loss of HIV-1 Vpr binding.

Secondary structure

......................................... 316
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q00403 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 9CC7E102526C2722

FASTA31634,833
        10         20         30         40         50         60 
MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA 

        70         80         90        100        110        120 
TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK YQNRRTMSSS DRAMMNAFKE 

       130        140        150        160        170        180 
ITTMADRINL PRNIVDRTNN LFKQVYEQKS LKGRANDAIA SACLYIACRQ EGVPRTFKEI 

       190        200        210        220        230        240 
CAVSRISKKE IGRCFKLILK ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV 

       250        260        270        280        290        300 
ELDLVPGRSP ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF 

       310 
PTDFKFDTPV DKLPQL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a human gene encoding the general transcription initiation factor IIB."
Ha I., Lane W.S., Reinberg D.
Nature 352:689-695(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence of general transcription factor TFIIB and relationships to other initiation factors."
Malik S., Hisatake K., Sumimoto H., Horikoshi M., Roeder R.G.
Proc. Natl. Acad. Sci. U.S.A. 88:9553-9557(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. expand/collapse author list , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N.
Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
[7]"Members of the steroid hormone receptor superfamily interact with TFIIB (S300-II)."
Ing N.H., Beekman J.M., Tsai S.Y., Tsai M.J., O'Malley B.W.
J. Biol. Chem. 267:17617-17623(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-316.
[8]"Isolation and characterization of a cDNA encoding a new type of human transcription elongation factor S-II."
Umehara T., Kida S., Yamamoto T., Horikoshi M.
Gene 167:297-302(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TCEA2.
[9]"The Epstein-Barr virus nuclear protein 2 acidic domain can interact with TFIIB, TAF40, and RPA70 but not with TATA-binding protein."
Tong X., Wang F., Thut C.J., Kieff E.
J. Virol. 69:585-588(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EBV EBNA2.
[10]"The human immunodeficiency virus type 1 Vpr transactivator: cooperation with promoter-bound activator domains and binding to TFIIB."
Agostini I., Navarro J.-M., Rey F., Bouhamdan M., Spire B., Vigne R., Sire J.
J. Mol. Biol. 261:599-606(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HIV-1 VPR.
[11]"The HIV-1 Vpr co-activator induces a conformational change in TFIIB."
Agostini I., Navarro J.M., Bouhamdan M., Willetts K., Rey F., Spire B., Vigne R., Pomerantz R., Sire J.
FEBS Lett. 450:235-239(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF 52-GLU--SER-56.
[12]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[14]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Solution structure of the C-terminal core domain of human TFIIB: similarity to cyclin A and interaction with TATA-binding protein."
Bagby S., Kim S., Maldonado E., Tong K.I., Reinberg D., Ikura M.
Cell 82:857-867(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 111-316.
[16]"Crystal structure of a TFIIB-TBP-TATA-element ternary complex."
Nikolov D.B., Chen H., Halay E.D., Usheva A.A., Hisatake K., Lee D.K., Roeder R.G., Burley S.K.
Nature 377:119-128(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 112-316.
[17]"Structure of a (Cys3His) zinc ribbon, a ubiquitous motif in archaeal and eucaryal transcription."
Chen H.T., Legault P., Glushka J., Omichinski J.G., Scott R.A.
Protein Sci. 9:1743-1752(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 2-59.
[18]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] GLN-132.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59268 mRNA. Translation: CAA41958.1.
M76766 mRNA. Translation: AAA61149.1.
AK289822 mRNA. Translation: BAF82511.1.
AB451296 mRNA. Translation: BAG70110.1.
AL445991 Genomic DNA. Translation: CAI41341.1.
BC020597 mRNA. Translation: AAH20597.1.
S44184 mRNA. Translation: AAB23144.1.
CCDSCCDS715.1.
PIRTWHU2B. S17654.
RefSeqNP_001505.1. NM_001514.5.
UniGeneHs.481852.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1C9BX-ray2.65A/E/I/M/Q110-316[»]
1DL6NMR-A2-59[»]
1RLYNMR-A1-60[»]
1RO4NMR-A1-60[»]
1TFBNMR-A112-316[»]
1VOLX-ray2.70A113-316[»]
2PHGNMR-A112-316[»]
ProteinModelPortalQ00403.
SMRQ00403. Positions 2-316.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109214. 64 interactions.
DIPDIP-1077N.
IntActQ00403. 25 interactions.
MINTMINT-156550.
STRING9606.ENSP00000359531.

PTM databases

PhosphoSiteQ00403.

Polymorphism databases

DMDM135629.

Proteomic databases

MaxQBQ00403.
PaxDbQ00403.
PeptideAtlasQ00403.
PRIDEQ00403.

Protocols and materials databases

DNASU2959.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000370500; ENSP00000359531; ENSG00000137947.
GeneID2959.
KEGGhsa:2959.
UCSCuc001dmo.4. human.

Organism-specific databases

CTD2959.
GeneCardsGC01M089318.
HGNCHGNC:4648. GTF2B.
HPAHPA061626.
MIM189963. gene.
neXtProtNX_Q00403.
PharmGKBPA29035.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1405.
HOGENOMHOG000108634.
HOVERGENHBG017914.
InParanoidQ00403.
KOK03124.
OMAYMASHIR.
OrthoDBEOG78WKS2.
PhylomeDBQ00403.
TreeFamTF105953.

Enzyme and pathway databases

ReactomeREACT_116125. Disease.
REACT_1788. Transcription.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressQ00403.
BgeeQ00403.
CleanExHS_GTF2B.
GenevestigatorQ00403.

Family and domain databases

Gene3D1.10.472.10. 2 hits.
InterProIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERPTHR11618. PTHR11618. 1 hit.
PfamPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSPR00685. TIFACTORIIB.
SMARTSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMSSF47954. SSF47954. 2 hits.
PROSITEPS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGTF2B. human.
EvolutionaryTraceQ00403.
GeneWikiTranscription_factor_II_B.
GenomeRNAi2959.
NextBio11730.
PMAP-CutDBQ00403.
PROQ00403.
SOURCESearch...

Entry information

Entry nameTF2B_HUMAN
AccessionPrimary (citable) accession number: Q00403
Secondary accession number(s): A8K1A7, Q5JS30
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 9, 2014
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM