Reviewed, UniProtKB/Swiss-Prot Q00393 (FRHG_METVO)

Names and origin

Protein names

Recommended name:
    Coenzyme F420 hydrogenase subunit gamma
    EC=1.12.98.1
Alternative name(s):
    8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma
      Short name=FRH

Gene names

Name:	frhG
Synonyms:	frcG

Organism

Methanococcus voltae

Taxonomic identifier

2188 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.
Catalytic activity	H2 + coenzyme F420 = reduced coenzyme F420.
Cofactor	Nickel. Iron-sulfur. FAD.
Subunit structure	Complex of alpha, beta and gamma subunits.
Sequence similarities	Belongs to the frhG family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro coenzyme F420 hydrogenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Coenzyme F420 hydrogenase subunit gamma		PRO_0000159234
Regions
	Domain	182 – 210	29	4Fe-4S ferredoxin-type 1
	Domain	211 – 241	31	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	191	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	194	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	197	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	201	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	220	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	223	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	226	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	230	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q00393-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 8929559D9A0129AD	FASTA	243	26,040
	10 20 30 40 50 60 MVKIAHIHMS GCTGCLISLT DTYEKLLDIL GSVELVYALT LTGEKTEITE TDDKILIERD 70 80 90 100 110 120 IPGGIDIALV EGSVCLDDHH SMDDILTTRK NSKKSLVALG ACAASGGVTR FRRVGQMSQP 130 140 150 160 170 180 SHASFVPIGD VIKVDLALPG CPPSTESIVK LLTAALEGDM EYLEPFAEIA KYGKDACGCD 190 200 210 220 230 240 VIKEVINKSL CMGCGTCAAA CQVNAIDMVE AKPNINSEFC IKCGICSAQC PRVRFPEIIR KLE

References

[1]

"Methanococcus voltae harbors four gene clusters potentially encoding two [NiFe] and two [NiFeSe] hydrogenases, each of the cofactor F420-reducing or F420-non-reducing types." Halboth S., Klein A. Mol. Gen. Genet. 233:217-224(1992) [PubMed: 1603063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 1537 / PS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X61201 Genomic DNA. Translation: CAA43498.1.
PIR	S16723.
3D structure databases
HSSP	HSSP built from PDB template 1CLF based on UniProtKB P00195.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.98.1. 20957.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR001450. 4Fe4S_Fe_S_bd_subgr. IPR017900. 4Fe4S_Fe_S_CS. IPR017681. Coenz_F420_hydrogenase_gsu. IPR006137. NADH_UbQ_OxRdtase-like_20kDa. [Graphical view]
Pfam	PF00037. Fer4. 2 hits. PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR03294. FrhG. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRHG_METVO

Accession

Primary (citable) accession number: Q00393

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)