Q00391 (FRHB_METVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coenzyme F420 hydrogenase subunit beta
EC=1.12.98.1

Alternative name(s):
8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta
Short name=FRH

Gene names

Name:	frhB
Synonyms:	frcB

Organism

Methanococcus voltae

Taxonomic identifier

2188 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.
Catalytic activity	H2 + coenzyme F420 = reduced coenzyme F420.
Cofactor	Nickel. Iron-sulfur. FAD.
Subunit structure	Complex of alpha, beta and gamma subunits.
Sequence similarities	Belongs to the frhB family.

Ontologies

Keywords
Ligand	Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Molecular function	coenzyme F420 hydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	Coenzyme F420 hydrogenase subunit beta		PRO_0000159230

Sequences

	Sequence		Length	Mass (Da)
	Q00391 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: CA00DC1F6DAE46AA	FASTA	280	30,868
	10 20 30 40 50 60 MPFGNYKTAI SARATDKEIL KKAQDGGYVS AAHIYGLESK LLDGVSVAAD TEDGCNAAPK 70 80 90 100 110 120 VATTPEEVLN AAGAKYTVSP NVSVLKDAVR EYALEKVGIV GTPCQVQAIR KLIKYPMGFR 130 140 150 160 170 180 HTDSRIVMGI FCMENFSYEG MKAIVEEYAG IRMDDVLKTD IGKGKFWVYS KYGDVKSVKL 190 200 210 220 230 240 KDTHMYEQKS CHICTDYTAE LADISTGSVG SPDGWSTIFI RTEKGEAYIN KMVEAGVLET 250 260 270 280 KNIDDVKPGL DLVQKLSLQK KEKNEKEIVQ RRELGLPVPY

References

[1]

"Methanococcus voltae harbors four gene clusters potentially encoding two [NiFe] and two [NiFeSe] hydrogenases, each of the cofactor F420-reducing or F420-non-reducing types." Halboth S., Klein A. Mol. Gen. Genet. 233:217-224(1992) [PubMed: 1603063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33273 / DSM 1537 / NBRC 100457 / OCM 70 / PS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61201 Genomic DNA. Translation: CAA43499.1.
PIR	S16724.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR007516. Co_F420_Hydgase/DH_bsu_N. IPR017679. Coenz_F420_hydrogenase_bsu. IPR007525. FrhB_FdhB_C. [Graphical view]
Pfam	PF04432. FrhB_FdhB_C. 1 hit. PF04422. FrhB_FdhB_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR03289. FrhB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FRHB_METVO

Accession

Primary (citable) accession number: Q00391

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 28, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program