Reviewed, UniProtKB/Swiss-Prot Q00390 (FRHA_METVO)

Names and origin

Protein names

Recommended name:
    Coenzyme F420 hydrogenase subunit alpha
    EC=1.12.98.1
Alternative name(s):
    8-hydroxy-5-deazaflavin-reducing hydrogenase subunit alpha
      Short name=FRH

Gene names

Name:	frhA
Synonyms:	frcA

Organism

Methanococcus voltae

Taxonomic identifier

2188 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.
Catalytic activity	H2 + coenzyme F420 = reduced coenzyme F420.
Cofactor	Nickel. Iron-sulfur. FAD.
Subunit structure	Complex of alpha, beta and gamma subunits.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Ligand	FAD Metal-binding Nickel
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro coenzyme F420 hydrogenase activity Inferred from electronic annotation. Source: EC ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro iron-sulfur cluster binding Inferred from electronic annotation. Source: InterPro nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Coenzyme F420 hydrogenase subunit alpha		PRO_0000199724
Sites
	Metal binding	63	1	Nickel Potential
	Metal binding	66	1	Nickel Potential
	Metal binding	386	1	Nickel Potential
	Metal binding	389	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	Q00390-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 696C95DD94C4EE83	FASTA	398	44,612
	10 20 30 40 50 60 MGKTVEINPT TRHEGHTKLV LKVDDDGIVE KGNYLSVTPV RGFEKFLVGK PAEFAPIAVS 70 80 90 100 110 120 RFCGICPIAH ATSAVEAIED ACGIVPPKDG LLLRELTGLG NKMHSHPLHE FLIAPDFIPE 130 140 150 160 170 180 KDRVEYITRI QQMRKTGQYI VDTIGGEAIH APNIKVGGML KSITPSAVSK IYYKCKEFEK 190 200 210 220 230 240 LAKEQVEYLI PIFENRTLVD GTEIPEKLGY HDFGYLATDS TYGNRENIEQ KKVHEYTPYD 250 260 270 280 290 300 VYEKEVAVQA CQLFQEYNGR LMEVGPRARF AKFHDFKEKG AMAIHIARAY ENVIHVKRAM 310 320 330 340 350 360 EIIEELNVDG LTRAKDPILG DGEKLGLGVH EAARGHNTHQ ASIDEKGRIT YYNAIVATTW 370 380 390 NIPLIGKAVE AHYKFAEHVV RAYDPCVSCA TQHDILRL

References

[1]

"Methanococcus voltae harbors four gene clusters potentially encoding two [NiFe] and two [NiFeSe] hydrogenases, each of the cofactor F420-reducing or F420-non-reducing types." Halboth S., Klein A. Mol. Gen. Genet. 233:217-224(1992) [PubMed: 1603063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 1537 / PS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61201 Genomic DNA. Translation: CAA43496.1.
PIR	S16721.
3D structure databases
SMR	Q00390. Positions 5-392.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.98.1. 20957.
Family and domain databases
InterPro	IPR017682. Coenz_F420_hydrogenase_asu. IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 2 hits. [Graphical view]
TIGRFAMs	TIGR03295. frhA. 1 hit.
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRHA_METVO

Accession

Primary (citable) accession number: Q00390

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)