Q00384 (ALKH_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
KHG/KDPG aldolase

Including the following 2 domains:

1. 4-hydroxy-2-oxoglutarate aldolase
   EC=4.1.3.16
   Alternative name(s):
   2-keto-4-hydroxyglutarate aldolase
   Short name=KHG-aldolase
2. 2-dehydro-3-deoxy-phosphogluconate aldolase
   EC=4.1.2.14
   Alternative name(s):
   2-keto-3-deoxy-6-phosphogluconate aldolase
   Short name=KDPG-aldolase
   Phospho-2-dehydro-3-deoxygluconate aldolase
   Phospho-2-keto-3-deoxygluconate aldolase

Gene names

Name:	eda
Synonyms:	kdgA
Ordered Locus Names:	ZMO0997

Organism

Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) [Complete proteome] [HAMAP]

Taxonomic identifier

264203 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas › Zymomonas mobilis › Zymomonas mobilis subsp. mobilis

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate. 2-dehydro-3-deoxy-D-gluconate 6-phosphate = pyruvate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate: step 2/2. Carbohydrate metabolism; glyoxylate and dicarboxylate metabolism.
Subunit structure	Homotrimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the KHG/KDPG aldolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2-dehydro-3-deoxy-phosphogluconate aldolase activity Inferred from electronic annotation. Source: EC 4-hydroxy-2-oxoglutarate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	KHG/KDPG aldolase		PRO_0000201044
Sites
	Active site	41	1	By similarity
	Active site	45	1	By similarity
	Active site	128	1	Schiff-base intermediate with KHG or pyruvate By similarity
Experimental info
	Sequence conflict	1 – 4	4	MRDI → M in CAA41258. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q00384 [UniParc]. Last modified February 15, 2005. Version 2. Checksum: 1685B45A7DFB57B4	FASTA	208	21,505
	10 20 30 40 50 60 MRDIDSVMRL APVMPVLVIE DIADAKPIAE ALVAGGLNVL EVTLRTPCAL EAIKIMKEVP 70 80 90 100 110 120 GAVVGAGTVL NAKMLDQAQE AGCEFFVSPG LTADLGKHAV AQKAALLPGV ANAADVMLGL 130 140 150 160 170 180 DLGLDRFKFF PAENIGGLPA LKSMASVFRQ VRFCPTGGIT PTSAPKYLEN PSILCVGGSW 190 200 VVPAGKPDVA KITALAKEAS AFKRAAVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, characterization and expression of the Zymononas mobilis eda gene that encodes 2-keto-3-deoxy-6-phosphogluconate aldolase of the Entner-Doudoroff pathway." Conway T., Fliege R., Jones-Kilpatrick D., Liu J., Barnell W.O., Egan S.E. Mol. Microbiol. 5:2901-2911(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.
[2]	"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X58364 Genomic DNA. Translation: CAA41258.1. AE008692 Genomic DNA. Translation: AAV89621.1.
RefSeq	YP_162732.1. NC_006526.2.
3D structure databases
ProteinModelPortal	Q00384.
ModBase	Search...
Protein-protein interaction databases
STRING	264203.ZMO0997.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV89621; AAV89621; ZMO0997.
GeneID	3188481.
KEGG	zmo:ZMO0997.
PATRIC	32567292. VBIZymMob102260_0944.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0800.
HOGENOM	HOG000233114.
KO	K01625.
OMA	EHAKKKG.
ProtClustDB	CLSK2524748.
Enzyme and pathway databases
UniPathway	UPA00227. UPA00856; UER00829.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR000887. Aldlse_KDPG_KHG. IPR013785. Aldolase_TIM. [Graphical view]
Pfam	PF01081. Aldolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01182. eda. 1 hit.
PROSITE	PS00159. ALDOLASE_KDPG_KHG_1. 1 hit. PS00160. ALDOLASE_KDPG_KHG_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALKH_ZYMMO

Accession

Primary (citable) accession number: Q00384
Secondary accession number(s): Q5NNT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program