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Q00384 (ALKH_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
KHG/KDPG aldolase

Including the following 2 domains:

  1. 4-hydroxy-2-oxoglutarate aldolase
    EC=4.1.3.16
    Alternative name(s):
    2-keto-4-hydroxyglutarate aldolase
    Short name=KHG-aldolase
  2. 2-dehydro-3-deoxy-phosphogluconate aldolase
    EC=4.1.2.14
    Alternative name(s):
    2-keto-3-deoxy-6-phosphogluconate aldolase
    Short name=KDPG-aldolase
    Phospho-2-dehydro-3-deoxygluconate aldolase
    Phospho-2-keto-3-deoxygluconate aldolase
Gene names
Name:eda
Synonyms:kdgA
Ordered Locus Names:ZMO0997
OrganismZymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) [Complete proteome] [HAMAP]
Taxonomic identifier264203 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeZymomonas

Protein attributes

Sequence length208 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate.

2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate.

Pathway

Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate: step 2/2.

Carbohydrate metabolism; glyoxylate and dicarboxylate metabolism.

Subunit structure

Homotrimer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the KHG/KDPG aldolase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Multifunctional enzyme
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function2-dehydro-3-deoxy-phosphogluconate aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

4-hydroxy-2-oxoglutarate aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 208208KHG/KDPG aldolase
PRO_0000201044

Sites

Active site411 By similarity
Active site451 By similarity
Active site1281Schiff-base intermediate with KHG or pyruvate By similarity

Experimental info

Sequence conflict1 – 44MRDI → M in CAA41258. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q00384 [UniParc].

Last modified February 15, 2005. Version 2.
Checksum: 1685B45A7DFB57B4

FASTA20821,505
        10         20         30         40         50         60 
MRDIDSVMRL APVMPVLVIE DIADAKPIAE ALVAGGLNVL EVTLRTPCAL EAIKIMKEVP 

        70         80         90        100        110        120 
GAVVGAGTVL NAKMLDQAQE AGCEFFVSPG LTADLGKHAV AQKAALLPGV ANAADVMLGL 

       130        140        150        160        170        180 
DLGLDRFKFF PAENIGGLPA LKSMASVFRQ VRFCPTGGIT PTSAPKYLEN PSILCVGGSW 

       190        200 
VVPAGKPDVA KITALAKEAS AFKRAAVA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, characterization and expression of the Zymononas mobilis eda gene that encodes 2-keto-3-deoxy-6-phosphogluconate aldolase of the Entner-Doudoroff pathway."
Conway T., Fliege R., Jones-Kilpatrick D., Liu J., Barnell W.O., Egan S.E.
Mol. Microbiol. 5:2901-2911(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.
[2]"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4."
Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. expand/collapse author list , Kang H.L., Lee S.Y., Lee K.J., Kang H.S.
Nat. Biotechnol. 23:63-68(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X58364 Genomic DNA. Translation: CAA41258.1.
AE008692 Genomic DNA. Translation: AAV89621.1.
RefSeqYP_162732.1. NC_006526.2.

3D structure databases

ProteinModelPortalQ00384.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264203.ZMO0997.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV89621; AAV89621; ZMO0997.
GeneID3188481.
KEGGzmo:ZMO0997.
PATRIC32567292. VBIZymMob102260_0944.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0800.
HOGENOMHOG000233114.
KOK01625.
OMASWLVPND.
OrthoDBEOG67DPKT.

Enzyme and pathway databases

UniPathwayUPA00227.
UPA00856; UER00829.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR000887. Aldlse_KDPG_KHG.
IPR013785. Aldolase_TIM.
[Graphical view]
PfamPF01081. Aldolase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01182. eda. 1 hit.
PROSITEPS00159. ALDOLASE_KDPG_KHG_1. 1 hit.
PS00160. ALDOLASE_KDPG_KHG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALKH_ZYMMO
AccessionPrimary (citable) accession number: Q00384
Secondary accession number(s): Q5NNT9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 15, 2005
Last modified: May 14, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways