Protein phosphatase PP2A regulatory subunit B - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q00362 (2ABA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 123. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein phosphatase PP2A regulatory subunit B

Alternative name(s):
Cell division control protein 55
PR55

Gene names

Name:	CDC55
Ordered Locus Names:	YGL190C
ORF Names:	G1345

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphatase 2A affects a variety of biological processes in the cell such as transcription, cell cycle progression and cellular morphogenesis, and provides an initial identification of critical substrates for this phosphatase. The regulatory subunit may direct the catalytic subunit to distinct, albeit overlapping, subsets of substrates.
Subunit structure	PP2A exists in several trimeric forms, all of which consist of a core composed of a catalytic subunit associated with a 65 kDa (PR65) (Subunit A) and a 55 kDa (PR55) (Subunit B) regulatory subunit. Interacts with YND1; this interaction mediates adenovirus E4orf4 (early region 4 open reading frame 4) induced toxicity and is disrupted by adenovirus E4orf4, which remains associated with both CDC55 and YND1. Ref.6
Miscellaneous	Present with 8600 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the phosphatase 2A regulatory subunit B family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Cell cycle
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell shape checkpoint Inferred from genetic interaction PubMed 20980617. Source: SGD negative regulation of exit from mitosis Inferred from mutant phenotype PubMed 16314395. Source: SGD positive regulation of G2/M transition of mitotic cell cycle Inferred from mutant phenotype PubMed 21536748. Source: SGD protein dephosphorylation Traceable author statement PubMed 9001215. Source: SGD signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cellular bud neck Inferred from direct assay PubMed 12388751. Source: SGD cellular bud tip Inferred from direct assay PubMed 12388751. Source: SGD cytoplasm Inferred from direct assay PubMed 21536748. Source: SGD nucleus Inferred from direct assay PubMed 12388751. Source: SGD protein phosphatase type 2A complex Inferred from electronic annotation. Source: InterPro
Molecular_function	protein phosphatase type 2A regulator activity Inferred from physical interaction PubMed 11038366. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ESP1	Q03018	2	EBI-1942,EBI-6657
ZDS2	P54786	9	EBI-1942,EBI-29637

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 526

526

Protein phosphatase PP2A regulatory subunit B

PRO_0000071442

Regions

Repeat

23 – 62

WD 1

Repeat

82 – 123

WD 2

Repeat

182 – 220

WD 3

Repeat

231 – 271

WD 4

Repeat

290 – 328

WD 5

Repeat

345 – 386

WD 6

Repeat

495 – 525

WD 7

Compositional bias

416 – 419

Poly-Ser

Amino acid modifications

Modified residue

124

Phosphoserine Ref.7

Modified residue

155

Phosphoserine Ref.7

Modified residue

440

Phosphoserine Ref.7

Experimental info

Sequence conflict

500

I → N in AAA34482. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q00362 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 6DA12C2805FA6A82
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FASTA

526

59,662

        10         20         30         40         50         60 
MAQNNFDFKF SQCFGDKADI VVTEADLITA VEFDYTGNYL ATGDKGGRVV LFERSNSRHC 

        70         80         90        100        110        120 
EYKFLTEFQS HDAEFDYLKS LEIEEKINEI KWLRPTQRSH FLLSTNDKTI KLWKVYEKNI 

       130        140        150        160        170        180 
KLVSQNNLTE GVTFAKKGKP DNHNSRGGSV RAVLSLQSLK LPQLSQHDKI IAATPKRIYS 

       190        200        210        220        230        240 
NAHTYHINSI SLNSDQETFL SADDLRINLW NLDIPDQSFN IVDIKPTNME ELTEVITSAE 

       250        260        270        280        290        300 
FHPQECNLFM YSSSKGTIKL CDMRQNSLCD NKTKTFEEYL DPINHNFFTE ITSSISDIKF 

       310        320        330        340        350        360 
SPNGRYIASR DYLTVKIWDV NMDNKPLKTI NIHEQLKERL SDTYENDAIF DKFEVNFSGD 

       370        380        390        400        410        420 
SSSVMTGSYN NNFMIYPNVV TSGDNDNGIV KTFDEHNAPN SNSNKNIHNS IQNKDSSSSG 

       430        440        450        460        470        480 
NSHKRRSNGR NTGMVGSSNS SRSSIAGGEG ANSEDSGTEM NEIVLQADKT AFRNKRYGSL 

       490        500        510        520 
AQRSARNKDW GDDIDFKKNI LHFSWHPREN SIAVAATNNL FIFSAL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CDC55, a Saccharomyces cerevisiae gene involved in cellular morphogenesis: identification, characterization, and homology to the B subunit of mammalian type 2A protein phosphatase." Healy A.M., Zolnierowicz S., Stapleton A.E., Goebl M., Depaoli-Roach A.A., Pringle J.R. Mol. Cell. Biol. 11:5767-5780(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae." Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V. Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"YND1 interacts with CDC55 and is a novel mediator of E4orf4-induced toxicity." Maoz T., Koren R., Ben-Ari I., Kleinberger T. J. Biol. Chem. 280:41270-41277(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH YND1.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124; SER-155 AND SER-440, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M72716 Genomic DNA. Translation: AAA34482.1. X91837 Genomic DNA. Translation: CAA62954.1. X91489 Genomic DNA. Translation: CAA62785.1. Z72712 Genomic DNA. Translation: CAA96902.1. BK006941 Genomic DNA. Translation: DAA07925.1.
PIR	A41698.
RefSeq	NP_011325.1. NM_001181055.1.
3D structure databases
ProteinModelPortal	Q00362.
SMR	Q00362. Positions 4-376, 492-523.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2281N.
IntAct	Q00362. 54 interactions.
MINT	MINT-542720.
STRING	4932.YGL190C.
Proteomic databases
PaxDb	Q00362.
PeptideAtlas	Q00362.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL190C; YGL190C; YGL190C.
GeneID	852685.
KEGG	sce:YGL190C.
Organism-specific databases
CYGD	YGL190c.
SGD	S000003158. CDC55.
Phylogenomic databases
eggNOG	COG5170.
GeneTree	ENSGT00390000006311.
HOGENOM	HOG000089745.
KO	K04354.
OMA	GEGANSE.
OrthoDB	EOG44QX8D.
Enzyme and pathway databases
BioCyc	YEAST:YGL190C-MONOMER.
Gene expression databases
Genevestigator	Q00362.
GermOnline	YGL190C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 3 hits.
InterPro	IPR000009. PP2A_PR55. IPR018067. PP2A_PR55_CS. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
PANTHER	PTHR11871. PTHR11871. 1 hit.
Pfam	PF00400. WD40. 1 hit. [Graphical view]
PIRSF	PIRSF037309. PP2A_PR55. 1 hit.
PRINTS	PR00600. PP2APR55.
SMART	SM00320. WD40. 5 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS01024. PR55_1. 1 hit. PS01025. PR55_2. 1 hit. PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	972008.

Entry information

Entry name

2ABA_YEAST

Accession

Primary (citable) accession number: Q00362
Secondary accession number(s): D6VTW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents