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Q00335

- MU2_REOVL

UniProt

Q00335 - MU2_REOVL

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Protein
Microtubule-associated protein mu-2
Gene
M1
Organism
Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Minor inner capsid (core) component. Displays NTPase and RNA 5'-triphosphatase (RTPase) activities. ATP is the preferred substrate for hydrolysis. May function as a cofactor of polymerase lambda-3. Associates with microtubules and plays a role in the formation, structural organization and morphology of viral inclusions, where the assembly of cores and the replication of viral RNA occur. Together with mu-NS, recruits the other core proteins to these inclusions.1 Publication

Cofactori

Divalent cations.

GO - Molecular functioni

  1. structural molecule activity Source: InterPro

GO - Biological processi

  1. suppression by virus of host IRF9 activity Source: UniProtKB-KW
  2. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF9 by virus, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated protein mu-2
Short name:
Mu2
Gene namesi
Name:M1
OrganismiReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Taxonomic identifieri10884 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000007253: Genome

Subcellular locationi

Virion Reviewed prediction. Host cytoplasmhost cytoskeleton By similarity
Note: Found in the inner capsid (12 copies). Associates with microtubules By similarity.

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cytoskeleton Source: UniProtKB-SubCell
  3. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, Host cytoskeleton, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi415 – 4151K → A: Loss of ATPase activity; when associated with A-419. No effect on microtubules association. 1 Publication
Mutagenesisi419 – 4191K → A: Loss of ATPase activity; when associated with A-415. No effect on microtubules association. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 736736Microtubule-associated protein mu-2
PRO_0000222747Add
BLAST

Interactioni

Subunit structurei

Interacts with protein mu-NS; in viral inclusions. Interacts with polymerase lambda-3; this interaction stimulates the ATPase activity of mu-2.2 Publications

Protein-protein interaction databases

IntActiQ00335. 15 interactions.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR012494. Reovirus_Mu2.
[Graphical view]
PfamiPF07781. Reovirus_Mu2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00335-1 [UniParc]FASTAAdd to Basket

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MAYIAVPAVV DSRSSEAIGL LESFGVDAGA DANDVSYQDH DYVLDQLQYM    50
LDGYEAGDVI DALVHKNWLH HSVYCLLPPK SQLLEYWKSN PSVIPDNVDR 100
RLRKRLMLKK DLRKDDEYNQ LARAFKISDV YAPLISSTTS PMTMIQNLNQ 150
GEIVYTTTDR VIGARILLYA PRKYYASTLS FTMTKCIIPF GKEVGRVPHS 200
RFNVGTFPSI ATPKCFVMSG VDIESIPNEF IKLFYQRVKS VHANILNDIS 250
PQIVSDMINR KRLRVHTPSD RRAAQLMHLP YHVKRGASHV DVYKVDVVDV 300
LLEVVDVADG LRNVSRKLTM HTVPVCILEM LGIEIADYCI RQEDGMFTDW 350
FLLLTMLSDG LTDRRTHCQY LINPSSVPPD VILNISITGF INRHTIDVMP 400
DIYDFVKPIG AVLPKGSFKS TIMRVLDSIS ILGVQIMPRA HVVDSDEVGE 450
QMEPTFEHAV MEIYKGIAGV DSLDDLIKWV LNSDLIPHDD RLGQLFQAFL 500
PLAKDLLAPM ARKFYDNSMS EGRLLTFAHA DSELLNANYF GHLLRLKIPY 550
ITEVNLMIRK NREGGELFQL VLSYLYKMYA TSAQPKWFGS LLRLLICPWL 600
HMEKLIGEAD PASTSAEIGW HIPREQLMQD GWCGCEDGFI PYVSIRAPRL 650
VMEELMEKNW GQYHAQVIVT DQLVVGEPRR VSAKAVIKGN HLPVKLVSRF 700
ACFTLTAKYE MRLSCGHSTG RGAAYNARLA FRSDLA 736
Length:736
Mass (Da):83,266
Last modified:December 1, 1992 - v1
Checksum:i41508441FA4FA784
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti302 – 3021L → F in AAL99936. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59945 Genomic RNA. Translation: CAA42570.1.
AF461682 mRNA. Translation: AAL99936.1.
PIRiS23654.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59945 Genomic RNA. Translation: CAA42570.1 .
AF461682 mRNA. Translation: AAL99936.1 .
PIRi S23654.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q00335. 15 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR012494. Reovirus_Mu2.
[Graphical view ]
Pfami PF07781. Reovirus_Mu2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence comparison of the M1 genome segment of reovirus type 1 Lang and type 3 Dearing."
    Zou S., Brown E.G.
    Virus Res. 22:159-164(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Reovirus core protein mu2 determines the filamentous morphology of viral inclusion bodies by interacting with and stabilizing microtubules."
    Parker J.S.L., Broering T.J., Kim J., Higgins D.E., Nibert M.L.
    J. Virol. 76:4483-4496(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Reovirus sigma NS protein localizes to inclusions through an association requiring the mu NS amino terminus."
    Miller C.L., Broering T.J., Parker J.S.L., Arnold M.M., Nibert M.L.
    J. Virol. 77:4566-4576(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PROTEIN MU-NS.
  4. "Nucleoside and RNA triphosphatase activities of orthoreovirus transcriptase cofactor mu2."
    Kim J., Parker J.S.L., Murray K.E., Nibert M.L.
    J. Biol. Chem. 279:4394-4403(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH POLYMERASE LAMBDA-3, CHARACTERIZATION OF NTPASE ACTIVITY, RTPASE ACTIVITY, MUTAGENESIS OF LYS-415 AND LSY-419.
  5. "Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions."
    Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L., Parker J.S.L.
    J. Virol. 78:1882-1892(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMU2_REOVL
AccessioniPrimary (citable) accession number: Q00335
Secondary accession number(s): Q8QT11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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