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Q00335

- MU2_REOVL

UniProt

Q00335 - MU2_REOVL

Protein

Microtubule-associated protein mu-2

Gene

M1

Organism
Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Minor inner capsid (core) component. Displays NTPase and RNA 5'-triphosphatase (RTPase) activities. ATP is the preferred substrate for hydrolysis. May function as a cofactor of polymerase lambda-3. Associates with microtubules and plays a role in the formation, structural organization and morphology of viral inclusions, where the assembly of cores and the replication of viral RNA occur. Together with mu-NS, recruits the other core proteins to these inclusions.1 Publication

    Cofactori

    Divalent cations.

    GO - Molecular functioni

    1. structural molecule activity Source: InterPro

    GO - Biological processi

    1. suppression by virus of host IRF9 activity Source: UniProtKB-KW
    2. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF9 by virus, Viral immunoevasion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Microtubule-associated protein mu-2
    Short name:
    Mu2
    Gene namesi
    Name:M1
    OrganismiReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
    Taxonomic identifieri10884 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
    Virus hostiMammalia [TaxID: 40674]
    ProteomesiUP000007253: Genome

    Subcellular locationi

    Virion Curated. Host cytoplasmhost cytoskeleton By similarity
    Note: Found in the inner capsid (12 copies). Associates with microtubules By similarity.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-KW
    2. host cytoskeleton Source: UniProtKB-SubCell
    3. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host cytoplasm, Host cytoskeleton, Virion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi415 – 4151K → A: Loss of ATPase activity; when associated with A-419. No effect on microtubules association. 1 Publication
    Mutagenesisi419 – 4191K → A: Loss of ATPase activity; when associated with A-415. No effect on microtubules association. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 736736Microtubule-associated protein mu-2PRO_0000222747Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with protein mu-NS; in viral inclusions. Interacts with polymerase lambda-3; this interaction stimulates the ATPase activity of mu-2.2 Publications

    Protein-protein interaction databases

    IntActiQ00335. 15 interactions.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the orthoreovirus mu-2 protein family.Curated

    Family and domain databases

    InterProiIPR012494. Reovirus_Mu2.
    [Graphical view]
    PfamiPF07781. Reovirus_Mu2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00335-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYIAVPAVV DSRSSEAIGL LESFGVDAGA DANDVSYQDH DYVLDQLQYM    50
    LDGYEAGDVI DALVHKNWLH HSVYCLLPPK SQLLEYWKSN PSVIPDNVDR 100
    RLRKRLMLKK DLRKDDEYNQ LARAFKISDV YAPLISSTTS PMTMIQNLNQ 150
    GEIVYTTTDR VIGARILLYA PRKYYASTLS FTMTKCIIPF GKEVGRVPHS 200
    RFNVGTFPSI ATPKCFVMSG VDIESIPNEF IKLFYQRVKS VHANILNDIS 250
    PQIVSDMINR KRLRVHTPSD RRAAQLMHLP YHVKRGASHV DVYKVDVVDV 300
    LLEVVDVADG LRNVSRKLTM HTVPVCILEM LGIEIADYCI RQEDGMFTDW 350
    FLLLTMLSDG LTDRRTHCQY LINPSSVPPD VILNISITGF INRHTIDVMP 400
    DIYDFVKPIG AVLPKGSFKS TIMRVLDSIS ILGVQIMPRA HVVDSDEVGE 450
    QMEPTFEHAV MEIYKGIAGV DSLDDLIKWV LNSDLIPHDD RLGQLFQAFL 500
    PLAKDLLAPM ARKFYDNSMS EGRLLTFAHA DSELLNANYF GHLLRLKIPY 550
    ITEVNLMIRK NREGGELFQL VLSYLYKMYA TSAQPKWFGS LLRLLICPWL 600
    HMEKLIGEAD PASTSAEIGW HIPREQLMQD GWCGCEDGFI PYVSIRAPRL 650
    VMEELMEKNW GQYHAQVIVT DQLVVGEPRR VSAKAVIKGN HLPVKLVSRF 700
    ACFTLTAKYE MRLSCGHSTG RGAAYNARLA FRSDLA 736
    Length:736
    Mass (Da):83,266
    Last modified:December 1, 1992 - v1
    Checksum:i41508441FA4FA784
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti302 – 3021L → F in AAL99936. (PubMed:11932414)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59945 Genomic RNA. Translation: CAA42570.1.
    AF461682 mRNA. Translation: AAL99936.1.
    PIRiS23654.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59945 Genomic RNA. Translation: CAA42570.1 .
    AF461682 mRNA. Translation: AAL99936.1 .
    PIRi S23654.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q00335. 15 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR012494. Reovirus_Mu2.
    [Graphical view ]
    Pfami PF07781. Reovirus_Mu2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence comparison of the M1 genome segment of reovirus type 1 Lang and type 3 Dearing."
      Zou S., Brown E.G.
      Virus Res. 22:159-164(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Reovirus core protein mu2 determines the filamentous morphology of viral inclusion bodies by interacting with and stabilizing microtubules."
      Parker J.S.L., Broering T.J., Kim J., Higgins D.E., Nibert M.L.
      J. Virol. 76:4483-4496(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Reovirus sigma NS protein localizes to inclusions through an association requiring the mu NS amino terminus."
      Miller C.L., Broering T.J., Parker J.S.L., Arnold M.M., Nibert M.L.
      J. Virol. 77:4566-4576(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PROTEIN MU-NS.
    4. "Nucleoside and RNA triphosphatase activities of orthoreovirus transcriptase cofactor mu2."
      Kim J., Parker J.S.L., Murray K.E., Nibert M.L.
      J. Biol. Chem. 279:4394-4403(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH POLYMERASE LAMBDA-3, CHARACTERIZATION OF NTPASE ACTIVITY, RTPASE ACTIVITY, MUTAGENESIS OF LYS-415 AND LSY-419.
    5. "Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions."
      Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L., Parker J.S.L.
      J. Virol. 78:1882-1892(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMU2_REOVL
    AccessioniPrimary (citable) accession number: Q00335
    Secondary accession number(s): Q8QT11
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3