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Q00335 (MU2_REOVL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Microtubule-associated protein mu-2

Short name=Mu2
Gene names
Name:M1
OrganismReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1) [Reference proteome]
Taxonomic identifier10884 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length736 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Minor inner capsid (core) component. Displays NTPase and RNA 5'-triphosphatase (RTPase) activities. ATP is the preferred substrate for hydrolysis. May function as a cofactor of polymerase lambda-3. Associates with microtubules and plays a role in the formation, structural organization and morphology of viral inclusions, where the assembly of cores and the replication of viral RNA occur. Together with mu-NS, recruits the other core proteins to these inclusions. Ref.5

Cofactor

Divalent cations.

Subunit structure

Interacts with protein mu-NS; in viral inclusions. Interacts with polymerase lambda-3; this interaction stimulates the ATPase activity of mu-2. Ref.3 Ref.4

Subcellular location

Virion Potential. Host cytoplasmhost cytoskeleton By similarity. Note: Found in the inner capsid (12 copies). Associates with microtubules By similarity.

Sequence similarities

Belongs to the orthoreovirus mu-2 protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 736736Microtubule-associated protein mu-2
PRO_0000222747

Experimental info

Mutagenesis4151K → A: Loss of ATPase activity; when associated with A-419. No effect on microtubules association. Ref.4
Mutagenesis4191K → A: Loss of ATPase activity; when associated with A-415. No effect on microtubules association. Ref.4
Sequence conflict3021L → F in AAL99936. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q00335 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 41508441FA4FA784

FASTA73683,266
        10         20         30         40         50         60 
MAYIAVPAVV DSRSSEAIGL LESFGVDAGA DANDVSYQDH DYVLDQLQYM LDGYEAGDVI 

        70         80         90        100        110        120 
DALVHKNWLH HSVYCLLPPK SQLLEYWKSN PSVIPDNVDR RLRKRLMLKK DLRKDDEYNQ 

       130        140        150        160        170        180 
LARAFKISDV YAPLISSTTS PMTMIQNLNQ GEIVYTTTDR VIGARILLYA PRKYYASTLS 

       190        200        210        220        230        240 
FTMTKCIIPF GKEVGRVPHS RFNVGTFPSI ATPKCFVMSG VDIESIPNEF IKLFYQRVKS 

       250        260        270        280        290        300 
VHANILNDIS PQIVSDMINR KRLRVHTPSD RRAAQLMHLP YHVKRGASHV DVYKVDVVDV 

       310        320        330        340        350        360 
LLEVVDVADG LRNVSRKLTM HTVPVCILEM LGIEIADYCI RQEDGMFTDW FLLLTMLSDG 

       370        380        390        400        410        420 
LTDRRTHCQY LINPSSVPPD VILNISITGF INRHTIDVMP DIYDFVKPIG AVLPKGSFKS 

       430        440        450        460        470        480 
TIMRVLDSIS ILGVQIMPRA HVVDSDEVGE QMEPTFEHAV MEIYKGIAGV DSLDDLIKWV 

       490        500        510        520        530        540 
LNSDLIPHDD RLGQLFQAFL PLAKDLLAPM ARKFYDNSMS EGRLLTFAHA DSELLNANYF 

       550        560        570        580        590        600 
GHLLRLKIPY ITEVNLMIRK NREGGELFQL VLSYLYKMYA TSAQPKWFGS LLRLLICPWL 

       610        620        630        640        650        660 
HMEKLIGEAD PASTSAEIGW HIPREQLMQD GWCGCEDGFI PYVSIRAPRL VMEELMEKNW 

       670        680        690        700        710        720 
GQYHAQVIVT DQLVVGEPRR VSAKAVIKGN HLPVKLVSRF ACFTLTAKYE MRLSCGHSTG 

       730 
RGAAYNARLA FRSDLA 

« Hide

References

[1]"Nucleotide sequence comparison of the M1 genome segment of reovirus type 1 Lang and type 3 Dearing."
Zou S., Brown E.G.
Virus Res. 22:159-164(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Reovirus core protein mu2 determines the filamentous morphology of viral inclusion bodies by interacting with and stabilizing microtubules."
Parker J.S.L., Broering T.J., Kim J., Higgins D.E., Nibert M.L.
J. Virol. 76:4483-4496(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Reovirus sigma NS protein localizes to inclusions through an association requiring the mu NS amino terminus."
Miller C.L., Broering T.J., Parker J.S.L., Arnold M.M., Nibert M.L.
J. Virol. 77:4566-4576(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PROTEIN MU-NS.
[4]"Nucleoside and RNA triphosphatase activities of orthoreovirus transcriptase cofactor mu2."
Kim J., Parker J.S.L., Murray K.E., Nibert M.L.
J. Biol. Chem. 279:4394-4403(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH POLYMERASE LAMBDA-3, CHARACTERIZATION OF NTPASE ACTIVITY, RTPASE ACTIVITY, MUTAGENESIS OF LYS-415 AND LSY-419.
[5]"Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions."
Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L., Parker J.S.L.
J. Virol. 78:1882-1892(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59945 Genomic RNA. Translation: CAA42570.1.
AF461682 mRNA. Translation: AAL99936.1.
PIRS23654.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ00335. 15 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR012494. Reovirus_Mu2.
[Graphical view]
PfamPF07781. Reovirus_Mu2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMU2_REOVL
AccessionPrimary (citable) accession number: Q00335
Secondary accession number(s): Q8QT11
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families