Q00335 (MU2_REOVL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Microtubule-associated protein mu-2
Short name=Mu2

Gene names

Name:

M1

Organism

Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1) [Reference proteome]

Taxonomic identifier

10884 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Reoviridae › Spinareovirinae › Orthoreovirus › Mammalian orthoreovirus › Mammalian orthoreovirus 1

Virus host

Mammalia [TaxID: 40674]

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Minor inner capsid (core) component. Displays NTPase and RNA 5'-triphosphatase (RTPase) activities. ATP is the preferred substrate for hydrolysis. May function as a cofactor of polymerase lambda-3. Associates with microtubules and plays a role in the formation, structural organization and morphology of viral inclusions, where the assembly of cores and the replication of viral RNA occur. Together with mu-NS, recruits the other core proteins to these inclusions. Ref.5
Cofactor	Divalent cations.
Subunit structure	Interacts with protein mu-NS; in viral inclusions. Interacts with polymerase lambda-3; this interaction stimulates the ATPase activity of mu-2. Ref.3 Ref.4
Subcellular location	Virion Potential. Host cytoplasm › host cytoskeleton By similarity. Note: Found in the inner capsid (12 copies). Associates with microtubules By similarity.
Sequence similarities	Belongs to the orthoreovirus mu-2 protein family.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host IRF9 by virus Inhibition of host innate immune response by virus Inhibition of host interferon signaling pathway by virus Viral immunoevasion
Cellular component	Host cytoplasm Host cytoskeleton Virion
Molecular function	Capsid protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	suppression by virus of host IRF9 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 736	736	Microtubule-associated protein mu-2		PRO_0000222747
Experimental info
	Mutagenesis	415	1	K → A: Loss of ATPase activity; when associated with A-419. No effect on microtubules association. Ref.4
	Mutagenesis	419	1	K → A: Loss of ATPase activity; when associated with A-415. No effect on microtubules association. Ref.4
	Sequence conflict	302	1	L → F in AAL99936. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q00335 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 41508441FA4FA784	FASTA	736	83,266
	10 20 30 40 50 60 MAYIAVPAVV DSRSSEAIGL LESFGVDAGA DANDVSYQDH DYVLDQLQYM LDGYEAGDVI 70 80 90 100 110 120 DALVHKNWLH HSVYCLLPPK SQLLEYWKSN PSVIPDNVDR RLRKRLMLKK DLRKDDEYNQ 130 140 150 160 170 180 LARAFKISDV YAPLISSTTS PMTMIQNLNQ GEIVYTTTDR VIGARILLYA PRKYYASTLS 190 200 210 220 230 240 FTMTKCIIPF GKEVGRVPHS RFNVGTFPSI ATPKCFVMSG VDIESIPNEF IKLFYQRVKS 250 260 270 280 290 300 VHANILNDIS PQIVSDMINR KRLRVHTPSD RRAAQLMHLP YHVKRGASHV DVYKVDVVDV 310 320 330 340 350 360 LLEVVDVADG LRNVSRKLTM HTVPVCILEM LGIEIADYCI RQEDGMFTDW FLLLTMLSDG 370 380 390 400 410 420 LTDRRTHCQY LINPSSVPPD VILNISITGF INRHTIDVMP DIYDFVKPIG AVLPKGSFKS 430 440 450 460 470 480 TIMRVLDSIS ILGVQIMPRA HVVDSDEVGE QMEPTFEHAV MEIYKGIAGV DSLDDLIKWV 490 500 510 520 530 540 LNSDLIPHDD RLGQLFQAFL PLAKDLLAPM ARKFYDNSMS EGRLLTFAHA DSELLNANYF 550 560 570 580 590 600 GHLLRLKIPY ITEVNLMIRK NREGGELFQL VLSYLYKMYA TSAQPKWFGS LLRLLICPWL 610 620 630 640 650 660 HMEKLIGEAD PASTSAEIGW HIPREQLMQD GWCGCEDGFI PYVSIRAPRL VMEELMEKNW 670 680 690 700 710 720 GQYHAQVIVT DQLVVGEPRR VSAKAVIKGN HLPVKLVSRF ACFTLTAKYE MRLSCGHSTG 730 RGAAYNARLA FRSDLA

References

[1]	"Nucleotide sequence comparison of the M1 genome segment of reovirus type 1 Lang and type 3 Dearing." Zou S., Brown E.G. Virus Res. 22:159-164(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Reovirus core protein mu2 determines the filamentous morphology of viral inclusion bodies by interacting with and stabilizing microtubules." Parker J.S.L., Broering T.J., Kim J., Higgins D.E., Nibert M.L. J. Virol. 76:4483-4496(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Reovirus sigma NS protein localizes to inclusions through an association requiring the mu NS amino terminus." Miller C.L., Broering T.J., Parker J.S.L., Arnold M.M., Nibert M.L. J. Virol. 77:4566-4576(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PROTEIN MU-NS.
[4]	"Nucleoside and RNA triphosphatase activities of orthoreovirus transcriptase cofactor mu2." Kim J., Parker J.S.L., Murray K.E., Nibert M.L. J. Biol. Chem. 279:4394-4403(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH POLYMERASE LAMBDA-3, CHARACTERIZATION OF NTPASE ACTIVITY, RTPASE ACTIVITY, MUTAGENESIS OF LYS-415 AND LSY-419.
[5]	"Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions." Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L., Parker J.S.L. J. Virol. 78:1882-1892(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59945 Genomic RNA. Translation: CAA42570.1. AF461682 mRNA. Translation: AAL99936.1.
PIR	S23654.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q00335. 15 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR012494. Reovirus_Mu2. [Graphical view]
Pfam	PF07781. Reovirus_Mu2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MU2_REOVL

Accession

Primary (citable) accession number: Q00335
Secondary accession number(s): Q8QT11

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program