Q00330 (RFBK_SALMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphomannomutase
Short name=PMM
EC=5.4.2.8

Gene names

Name:	manB
Synonyms:	rfbK

Organism

Salmonella muenchen

Taxonomic identifier

596 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group C2 O antigen.
Catalytic activity	Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2. Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the phosphohexose mutase family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	GDP-mannose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway O antigen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: InterPro phosphomannomutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Phosphomannomutase		PRO_0000147825
Regions
	Transmembrane	30 – 46	17	Helical; Potential
	Transmembrane	265 – 284	20	Helical; Potential
Sites
	Active site	111	1	Phosphoserine intermediate By similarity
	Metal binding	111	1	Magnesium; via phosphate group By similarity
	Metal binding	245	1	Magnesium By similarity
	Metal binding	247	1	Magnesium By similarity
	Metal binding	249	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q00330 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 982E5B885083B893	FASTA	478	52,813
	10 20 30 40 50 60 MKNIYNTYDV INKSGINFGT SGARGLVTDF TPEVCARFTI SFLTVMQQRF SFTTVALAID 70 80 90 100 110 120 NRPSSYAMAQ ACAAALQEKG IKTVYYGVIP TPALAHQSIS DKVPAIMVTG SHIPFDRNGL 130 140 150 160 170 180 KFYRPDGEIT KDDENAIIHV DASFMQPKLE QLTISTIAAR NYILRYTSLF PMPFLKNKRI 190 200 210 220 230 240 GIYEHSSAGR DLYKTLFKML GATVVSLARS DEFVPIDTEA VSEDDRNKAI TWAKKYQLDA 250 260 270 280 290 300 IFSTDGDGDR PLIADEYGNW LRGDILGLLC SLELAADAVA IPVSCNSTIS SGNFFKHVER 310 320 330 340 350 360 TKIGSPYVIA AFAKLSANYN CIAGFEANGG FLLGSDVYIN QRLLKALPTR DALLPAIMLL 370 380 390 400 410 420 FGSKDKSISE LVKKLPARYT YSNRLQDISV KTSMSLINLG LTDQEDFLQY IGFNKHHILH 430 440 450 460 470 SDVTDGFRIT IDNNNIIHLR PSGNAPELRC YAEADSQEDA CNIVETVLSN IKSKLGRA

References

[1]

"Molecular analysis of the rfb gene cluster of Salmonella serovar muenchen (strain M67): the genetic basis of the polymorphism between groups C2 and B." Brown P.K., Romana L.K., Reeves P.R. Mol. Microbiol. 6:1385-1394(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M67.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61917 Genomic DNA. Translation: CAA43916.1.
PIR	S22622.
3D structure databases
ProteinModelPortal	Q00330.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00126; UER00424. UPA00281.
Family and domain databases
Gene3D	3.40.120.10. 1 hit.
InterPro	IPR005844. A-D-PHexomutase_a/b/a-I. IPR016055. A-D-PHexomutase_a/b/a-I/II/III. IPR005845. A-D-PHexomutase_a/b/a-II. IPR005846. A-D-PHexomutase_a/b/a-III. IPR005843. A-D-PHexomutase_C. IPR016066. A-D-PHexomutase_CS. [Graphical view]
Pfam	PF02878. PGM_PMM_I. 1 hit. PF02879. PGM_PMM_II. 1 hit. PF02880. PGM_PMM_III. 1 hit. PF00408. PGM_PMM_IV. 1 hit. [Graphical view]
SUPFAM	SSF53738. A-D-PHexomutase_a/b/a-I/II/III. 3 hits.
PROSITE	PS00710. PGM_PMM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFBK_SALMU

Accession

Primary (citable) accession number: Q00330

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program