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Q00330 (RFBK_SALMU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphomannomutase

Short name=PMM
EC=5.4.2.8
Gene names
Name:manB
Synonyms:rfbK
OrganismSalmonella muenchen
Taxonomic identifier596 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group C2 O antigen.

Catalytic activity

Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Pathway

Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.

Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Sequence similarities

Belongs to the phosphohexose mutase family.

Ontologies

Keywords
   Biological processLipopolysaccharide biosynthesis
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandMagnesium
Metal-binding
   Molecular functionIsomerase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological_processGDP-mannose biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

O antigen biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: InterPro

phosphomannomutase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 478478Phosphomannomutase
PRO_0000147825

Regions

Transmembrane30 – 4617Helical; Potential
Transmembrane265 – 28420Helical; Potential

Sites

Active site1111Phosphoserine intermediate By similarity
Metal binding1111Magnesium; via phosphate group By similarity
Metal binding2451Magnesium By similarity
Metal binding2471Magnesium By similarity
Metal binding2491Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q00330 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 982E5B885083B893

FASTA47852,813
        10         20         30         40         50         60 
MKNIYNTYDV INKSGINFGT SGARGLVTDF TPEVCARFTI SFLTVMQQRF SFTTVALAID 

        70         80         90        100        110        120 
NRPSSYAMAQ ACAAALQEKG IKTVYYGVIP TPALAHQSIS DKVPAIMVTG SHIPFDRNGL 

       130        140        150        160        170        180 
KFYRPDGEIT KDDENAIIHV DASFMQPKLE QLTISTIAAR NYILRYTSLF PMPFLKNKRI 

       190        200        210        220        230        240 
GIYEHSSAGR DLYKTLFKML GATVVSLARS DEFVPIDTEA VSEDDRNKAI TWAKKYQLDA 

       250        260        270        280        290        300 
IFSTDGDGDR PLIADEYGNW LRGDILGLLC SLELAADAVA IPVSCNSTIS SGNFFKHVER 

       310        320        330        340        350        360 
TKIGSPYVIA AFAKLSANYN CIAGFEANGG FLLGSDVYIN QRLLKALPTR DALLPAIMLL 

       370        380        390        400        410        420 
FGSKDKSISE LVKKLPARYT YSNRLQDISV KTSMSLINLG LTDQEDFLQY IGFNKHHILH 

       430        440        450        460        470 
SDVTDGFRIT IDNNNIIHLR PSGNAPELRC YAEADSQEDA CNIVETVLSN IKSKLGRA 

« Hide

References

[1]"Molecular analysis of the rfb gene cluster of Salmonella serovar muenchen (strain M67): the genetic basis of the polymorphism between groups C2 and B."
Brown P.K., Romana L.K., Reeves P.R.
Mol. Microbiol. 6:1385-1394(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: M67.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61917 Genomic DNA. Translation: CAA43916.1.
PIRS22622.

3D structure databases

ProteinModelPortalQ00330.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00126; UER00424.
UPA00281.

Family and domain databases

Gene3D3.30.310.50. 1 hit.
3.40.120.10. 1 hit.
InterProIPR005844. A-D-PHexomutase_a/b/a-I.
IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
IPR005845. A-D-PHexomutase_a/b/a-II.
IPR005846. A-D-PHexomutase_a/b/a-III.
IPR005843. A-D-PHexomutase_C.
IPR016066. A-D-PHexomutase_CS.
[Graphical view]
PfamPF02878. PGM_PMM_I. 1 hit.
PF02879. PGM_PMM_II. 1 hit.
PF02880. PGM_PMM_III. 1 hit.
PF00408. PGM_PMM_IV. 1 hit.
[Graphical view]
SUPFAMSSF53738. SSF53738. 3 hits.
SSF55957. SSF55957. 1 hit.
PROSITEPS00710. PGM_PMM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRFBK_SALMU
AccessionPrimary (citable) accession number: Q00330
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways