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Q00328 (GUX1_COCCA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exoglucanase 1

EC=3.2.1.91
Alternative name(s):
1,4-beta-cellobiohydrolase I
Beta-glucancellobiohydrolase I
Exocellobiohydrolase I
Exoglucanase I
Gene names
Name:CEL1
OrganismCochliobolus carbonum (Bipolaris zeicola)
Taxonomic identifier5017 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePleosporaceaeBipolaris

Protein attributes

Sequence length456 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.

Catalytic activity

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sequence similarities

Belongs to the glycosyl hydrolase 7 (cellulase C) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncellulose 1,4-beta-cellobiosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 456438Exoglucanase 1
PRO_0000007919

Sites

Active site2321Nucleophile By similarity
Active site2371Proton donor By similarity

Amino acid modifications

Glycosylation441N-linked (GlcNAc...) Potential
Glycosylation471N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q00328 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8014094907DEEA52

FASTA45648,304
        10         20         30         40         50         60 
MYRTLAFASL SLYGAARAQQ VGTSTAENHP KLTWQTCTGT GGTNCSNKSG SVVLDSNWRW 

        70         80         90        100        110        120 
AHNVGGYTNC YTGNSWSTQY CPDGDSCTKN CAIDGADYSG TYGITTSNNA LSLKFVTKGS 

       130        140        150        160        170        180 
FSSNIGSRTY LMETDTKYQM FNLINKEFTF DVDVSKLPCG LNGALYFVEM AADGGIGKGN 

       190        200        210        220        230        240 
NKAGAKYGTG YCDSQCPHDI KFINGKANVE GWNPSDADPN GGAGKIGACC PEMDIWEANS 

       250        260        270        280        290        300 
ISTAYTPHPC RGVGLQECSD AASCGDGSNR YDGQCDKDGC DFNSYRMGVK DFYGPGATLD 

       310        320        330        340        350        360 
TTKKMTVITQ FLGSGSSLSE IKRFYVQNGK VYKNSQSAVA GVTGNSITES FCTAQKKAFG 

       370        380        390        400        410        420 
DTSSFAALGG LNEMGASLAR GHVLIMSLWG DHAVNMLWLD STYPTDADPS KPGAARGTCP 

       430        440        450 
TTSGKPEDVE KNSPDATVVF SNIKFGPIGS TFAQPA 

« Hide

References

[1]"Characterization and disruption of a gene in the maize pathogen Cochliobolus carbonum encoding a cellulase lacking a cellulose binding domain and hinge region."
Sposato P., Ahn J., Walton J.D.
Mol. Plant Microbe Interact. 8:602-609(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Race 1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U25129 Genomic DNA. Translation: AAC49089.1.

3D structure databases

ProteinModelPortalQ00328.
SMRQ00328. Positions 19-452.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH7. Glycoside Hydrolase Family 7.
mycoCLAPCBH7A_COCCA.

Proteomic databases

PRIDEQ00328.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.70.100.10. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR001722. Glyco_hydro_7.
[Graphical view]
PfamPF00840. Glyco_hydro_7. 1 hit.
[Graphical view]
PRINTSPR00734. GLHYDRLASE7.
SUPFAMSSF49899. SSF49899. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGUX1_COCCA
AccessionPrimary (citable) accession number: Q00328
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: January 22, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries