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Q00328

- GUX1_COCCA

UniProt

Q00328 - GUX1_COCCA

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Protein

Exoglucanase 1

Gene

CEL1

Organism
Cochliobolus carbonum (Bipolaris zeicola)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei232 – 2321NucleophileBy similarity
Active sitei237 – 2371Proton donorBy similarity

GO - Molecular functioni

  1. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH7. Glycoside Hydrolase Family 7.
mycoCLAPiCBH7A_COCCA.

Names & Taxonomyi

Protein namesi
Recommended name:
Exoglucanase 1 (EC:3.2.1.91)
Alternative name(s):
1,4-beta-cellobiohydrolase I
Beta-glucancellobiohydrolase I
Exocellobiohydrolase I
Exoglucanase I
Gene namesi
Name:CEL1
OrganismiCochliobolus carbonum (Bipolaris zeicola)
Taxonomic identifieri5017 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePleosporaceaeBipolaris

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 456438Exoglucanase 1PRO_0000007919Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ00328.

Structurei

3D structure databases

ProteinModelPortaliQ00328.
SMRiQ00328. Positions 19-452.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.70.100.10. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001722. Glyco_hydro_7.
[Graphical view]
PfamiPF00840. Glyco_hydro_7. 1 hit.
[Graphical view]
PRINTSiPR00734. GLHYDRLASE7.
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00328-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYRTLAFASL SLYGAARAQQ VGTSTAENHP KLTWQTCTGT GGTNCSNKSG
60 70 80 90 100
SVVLDSNWRW AHNVGGYTNC YTGNSWSTQY CPDGDSCTKN CAIDGADYSG
110 120 130 140 150
TYGITTSNNA LSLKFVTKGS FSSNIGSRTY LMETDTKYQM FNLINKEFTF
160 170 180 190 200
DVDVSKLPCG LNGALYFVEM AADGGIGKGN NKAGAKYGTG YCDSQCPHDI
210 220 230 240 250
KFINGKANVE GWNPSDADPN GGAGKIGACC PEMDIWEANS ISTAYTPHPC
260 270 280 290 300
RGVGLQECSD AASCGDGSNR YDGQCDKDGC DFNSYRMGVK DFYGPGATLD
310 320 330 340 350
TTKKMTVITQ FLGSGSSLSE IKRFYVQNGK VYKNSQSAVA GVTGNSITES
360 370 380 390 400
FCTAQKKAFG DTSSFAALGG LNEMGASLAR GHVLIMSLWG DHAVNMLWLD
410 420 430 440 450
STYPTDADPS KPGAARGTCP TTSGKPEDVE KNSPDATVVF SNIKFGPIGS

TFAQPA
Length:456
Mass (Da):48,304
Last modified:November 1, 1996 - v1
Checksum:i8014094907DEEA52
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25129 Genomic DNA. Translation: AAC49089.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25129 Genomic DNA. Translation: AAC49089.1 .

3D structure databases

ProteinModelPortali Q00328.
SMRi Q00328. Positions 19-452.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH7. Glycoside Hydrolase Family 7.
mycoCLAPi CBH7A_COCCA.

Proteomic databases

PRIDEi Q00328.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.70.100.10. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR001722. Glyco_hydro_7.
[Graphical view ]
Pfami PF00840. Glyco_hydro_7. 1 hit.
[Graphical view ]
PRINTSi PR00734. GLHYDRLASE7.
SUPFAMi SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization and disruption of a gene in the maize pathogen Cochliobolus carbonum encoding a cellulase lacking a cellulose binding domain and hinge region."
    Sposato P., Ahn J., Walton J.D.
    Mol. Plant Microbe Interact. 8:602-609(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Race 1.

Entry informationi

Entry nameiGUX1_COCCA
AccessioniPrimary (citable) accession number: Q00328
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3