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Q00326 (MYRO_BRANA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myrosinase

EC=3.2.1.147
Alternative name(s):
Sinigrinase
Thioglucosidase
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.

Catalytic activity

A thioglucoside + H2O = a sugar + a thiol.

Subunit structure

Homodimer.

Subcellular location

Vacuole.

Tissue specificity

In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant.

Miscellaneous

It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
   Cellular componentVacuole
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentvacuole

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

thioglucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 By similarity
Chain21 – 548528Myrosinase
PRO_0000011774

Regions

Region484 – 4852Substrate binding By similarity

Sites

Active site4291Nucleophile By similarity
Metal binding761Zinc; shared with dimeric partner By similarity
Metal binding901Zinc; shared with dimeric partner By similarity
Binding site591Substrate By similarity
Binding site1611Substrate By similarity
Binding site2061Substrate By similarity
Binding site2071Ascorbate By similarity
Binding site2811Ascorbate By similarity
Binding site3521Substrate By similarity
Binding site4771Substrate By similarity

Amino acid modifications

Glycosylation1101N-linked (GlcNAc...) Potential
Glycosylation2401N-linked (GlcNAc...) Potential
Glycosylation3311N-linked (GlcNAc...) Potential
Glycosylation5201N-linked (GlcNAc...) Potential
Disulfide bond26 ↔ 458 By similarity
Disulfide bond34 ↔ 454 By similarity
Disulfide bond226 ↔ 236 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q00326 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: F3034C6A01CD732A

FASTA54862,736
        10         20         30         40         50         60 
MKLLHGLALV FLLAAASCKA DEEITCEENN PFTCSNTDIL SSKNFGKDFI FGVASSAYQI 

        70         80         90        100        110        120 
EGGRGRGVNV WDGFSHRYPE KAGSDLKNGD TTCESYTRWQ KDVDVMGELN ATGYRFSFAW 

       130        140        150        160        170        180 
SRIIPKGKVS RGVNQGGLDY YHKLIDALLE KNITPFVTLF HWDLPQTLQD EYEGFLDRQI 

       190        200        210        220        230        240 
IQDFKDYADL CFKEFGGKVK HWITINQLYT VPTRGYAIGT DAPGRCSPMV DTKHRCYGGN 

       250        260        270        280        290        300 
SSTEPYIVAH NQLLAHATVV DLYRTKYKFQ KGKIGPVMIT RWFLPFDESD PASIEAAERM 

       310        320        330        340        350        360 
NQFFHGWYME PLTKGRYPDI MRQIVGSRLP NFTEEEAELV AGSYDFLGLN YYVTQYAQPK 

       370        380        390        400        410        420 
PNPYPSETHT AMMDAGVKLT YDNSRGEFLG PLFVEDKVNG NSYYYPKGIY YVMDYFKTKY 

       430        440        450        460        470        480 
GDPLIYVTEN GFSTPSSENR EQAIADYKRI DYLCSHLCFL RKVIKEKGVN VRGYFAWALG 

       490        500        510        520        530        540 
DNYEFCKGFT VRFGLSYVNW EDLDDRNLKE SGKWYQRFIN GTVKNAVKQD FLRSSLSSQS 


QKKRFADA 

« Hide

References

[1]"Sequence of a cDNA clone encoding the enzyme myrosinase, and expression of myrosinase in different tissues of Brassica napus."
Falk A., Xue J., Lenman M., Rask L.
Plant Sci. 83:181-186(1992)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Svalofs Karat.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60214 mRNA. Translation: CAA42775.1.
PIRS26149.

3D structure databases

ProteinModelPortalQ00326.
SMRQ00326. Positions 23-520.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMYRO_BRANA
AccessionPrimary (citable) accession number: Q00326
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries