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Q00326

- MYRO_BRANA

UniProt

Q00326 - MYRO_BRANA

Protein

Myrosinase

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.

    Catalytic activityi

    A thioglucoside + H2O = a sugar + a thiol.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei59 – 591SubstrateBy similarity
    Metal bindingi76 – 761Zinc; shared with dimeric partnerBy similarity
    Metal bindingi90 – 901Zinc; shared with dimeric partnerBy similarity
    Binding sitei161 – 1611SubstrateBy similarity
    Binding sitei206 – 2061SubstrateBy similarity
    Binding sitei207 – 2071AscorbateBy similarity
    Binding sitei281 – 2811AscorbateBy similarity
    Binding sitei352 – 3521SubstrateBy similarity
    Active sitei429 – 4291NucleophilePROSITE-ProRule annotation
    Binding sitei477 – 4771SubstrateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. thioglucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myrosinase (EC:3.2.1.147)
    Alternative name(s):
    Sinigrinase
    Thioglucosidase
    OrganismiBrassica napus (Rape)
    Taxonomic identifieri3708 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

    Subcellular locationi

    GO - Cellular componenti

    1. vacuole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020By similarityAdd
    BLAST
    Chaini21 – 548528MyrosinasePRO_0000011774Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 458By similarity
    Disulfide bondi34 ↔ 454By similarity
    Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi226 ↔ 236By similarity
    Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi520 – 5201N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00326.
    SMRiQ00326. Positions 23-520.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni484 – 4852Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q00326-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLLHGLALV FLLAAASCKA DEEITCEENN PFTCSNTDIL SSKNFGKDFI    50
    FGVASSAYQI EGGRGRGVNV WDGFSHRYPE KAGSDLKNGD TTCESYTRWQ 100
    KDVDVMGELN ATGYRFSFAW SRIIPKGKVS RGVNQGGLDY YHKLIDALLE 150
    KNITPFVTLF HWDLPQTLQD EYEGFLDRQI IQDFKDYADL CFKEFGGKVK 200
    HWITINQLYT VPTRGYAIGT DAPGRCSPMV DTKHRCYGGN SSTEPYIVAH 250
    NQLLAHATVV DLYRTKYKFQ KGKIGPVMIT RWFLPFDESD PASIEAAERM 300
    NQFFHGWYME PLTKGRYPDI MRQIVGSRLP NFTEEEAELV AGSYDFLGLN 350
    YYVTQYAQPK PNPYPSETHT AMMDAGVKLT YDNSRGEFLG PLFVEDKVNG 400
    NSYYYPKGIY YVMDYFKTKY GDPLIYVTEN GFSTPSSENR EQAIADYKRI 450
    DYLCSHLCFL RKVIKEKGVN VRGYFAWALG DNYEFCKGFT VRFGLSYVNW 500
    EDLDDRNLKE SGKWYQRFIN GTVKNAVKQD FLRSSLSSQS QKKRFADA 548
    Length:548
    Mass (Da):62,736
    Last modified:December 1, 1992 - v1
    Checksum:iF3034C6A01CD732A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60214 mRNA. Translation: CAA42775.1.
    PIRiS26149.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60214 mRNA. Translation: CAA42775.1 .
    PIRi S26149.

    3D structure databases

    ProteinModelPortali Q00326.
    SMRi Q00326. Positions 23-520.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of a cDNA clone encoding the enzyme myrosinase, and expression of myrosinase in different tissues of Brassica napus."
      Falk A., Xue J., Lenman M., Rask L.
      Plant Sci. 83:181-186(1992)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Svalofs Karat.

    Entry informationi

    Entry nameiMYRO_BRANA
    AccessioniPrimary (citable) accession number: Q00326
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3