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Protein

Myrosinase

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei59SubstrateBy similarity1
Metal bindingi76Zinc; shared with dimeric partnerBy similarity1
Metal bindingi90Zinc; shared with dimeric partnerBy similarity1
Binding sitei161SubstrateBy similarity1
Binding sitei206SubstrateBy similarity1
Binding sitei207AscorbateBy similarity1
Binding sitei281AscorbateBy similarity1
Binding sitei352SubstrateBy similarity1
Active sitei429NucleophilePROSITE-ProRule annotation1
Binding sitei477SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Myrosinase (EC:3.2.1.147)
Alternative name(s):
Sinigrinase
Thioglucosidase
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
ChainiPRO_000001177421 – 548MyrosinaseAdd BLAST528

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 458By similarity
Disulfide bondi34 ↔ 454By similarity
Glycosylationi110N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi226 ↔ 236By similarity
Glycosylationi240N-linked (GlcNAc...)Sequence analysis1
Glycosylationi331N-linked (GlcNAc...)Sequence analysis1
Glycosylationi520N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliQ00326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni484 – 485Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00326-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLHGLALV FLLAAASCKA DEEITCEENN PFTCSNTDIL SSKNFGKDFI
60 70 80 90 100
FGVASSAYQI EGGRGRGVNV WDGFSHRYPE KAGSDLKNGD TTCESYTRWQ
110 120 130 140 150
KDVDVMGELN ATGYRFSFAW SRIIPKGKVS RGVNQGGLDY YHKLIDALLE
160 170 180 190 200
KNITPFVTLF HWDLPQTLQD EYEGFLDRQI IQDFKDYADL CFKEFGGKVK
210 220 230 240 250
HWITINQLYT VPTRGYAIGT DAPGRCSPMV DTKHRCYGGN SSTEPYIVAH
260 270 280 290 300
NQLLAHATVV DLYRTKYKFQ KGKIGPVMIT RWFLPFDESD PASIEAAERM
310 320 330 340 350
NQFFHGWYME PLTKGRYPDI MRQIVGSRLP NFTEEEAELV AGSYDFLGLN
360 370 380 390 400
YYVTQYAQPK PNPYPSETHT AMMDAGVKLT YDNSRGEFLG PLFVEDKVNG
410 420 430 440 450
NSYYYPKGIY YVMDYFKTKY GDPLIYVTEN GFSTPSSENR EQAIADYKRI
460 470 480 490 500
DYLCSHLCFL RKVIKEKGVN VRGYFAWALG DNYEFCKGFT VRFGLSYVNW
510 520 530 540
EDLDDRNLKE SGKWYQRFIN GTVKNAVKQD FLRSSLSSQS QKKRFADA
Length:548
Mass (Da):62,736
Last modified:December 1, 1992 - v1
Checksum:iF3034C6A01CD732A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60214 mRNA. Translation: CAA42775.1.
PIRiS26149.
RefSeqiNP_001302796.1. NM_001315867.1.
UniGeneiBna.2707.

Genome annotation databases

GeneIDi106382674.
KEGGibna:106382674.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60214 mRNA. Translation: CAA42775.1.
PIRiS26149.
RefSeqiNP_001302796.1. NM_001315867.1.
UniGeneiBna.2707.

3D structure databases

ProteinModelPortaliQ00326.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi106382674.
KEGGibna:106382674.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYRO_BRANA
AccessioniPrimary (citable) accession number: Q00326
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 5, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.