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Q00326

- MYRO_BRANA

UniProt

Q00326 - MYRO_BRANA

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Protein

Myrosinase

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei59 – 591Substrate By similarity
Metal bindingi76 – 761Zinc; shared with dimeric partner By similarity
Metal bindingi90 – 901Zinc; shared with dimeric partner By similarity
Binding sitei161 – 1611Substrate By similarity
Binding sitei206 – 2061Substrate By similarity
Binding sitei207 – 2071Ascorbate By similarity
Binding sitei281 – 2811Ascorbate By similarity
Binding sitei352 – 3521Substrate By similarity
Active sitei429 – 4291Nucleophile By similarity
Binding sitei477 – 4771Substrate By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. thioglucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Myrosinase (EC:3.2.1.147)
Alternative name(s):
Sinigrinase
Thioglucosidase
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

  1. vacuole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 By similarityAdd
BLAST
Chaini21 – 548528MyrosinasePRO_0000011774Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 458 By similarity
Disulfide bondi34 ↔ 454 By similarity
Glycosylationi110 – 1101N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi226 ↔ 236 By similarity
Glycosylationi240 – 2401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi331 – 3311N-linked (GlcNAc...) Reviewed prediction
Glycosylationi520 – 5201N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliQ00326.
SMRiQ00326. Positions 23-520.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni484 – 4852Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00326-1 [UniParc]FASTAAdd to Basket

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MKLLHGLALV FLLAAASCKA DEEITCEENN PFTCSNTDIL SSKNFGKDFI    50
FGVASSAYQI EGGRGRGVNV WDGFSHRYPE KAGSDLKNGD TTCESYTRWQ 100
KDVDVMGELN ATGYRFSFAW SRIIPKGKVS RGVNQGGLDY YHKLIDALLE 150
KNITPFVTLF HWDLPQTLQD EYEGFLDRQI IQDFKDYADL CFKEFGGKVK 200
HWITINQLYT VPTRGYAIGT DAPGRCSPMV DTKHRCYGGN SSTEPYIVAH 250
NQLLAHATVV DLYRTKYKFQ KGKIGPVMIT RWFLPFDESD PASIEAAERM 300
NQFFHGWYME PLTKGRYPDI MRQIVGSRLP NFTEEEAELV AGSYDFLGLN 350
YYVTQYAQPK PNPYPSETHT AMMDAGVKLT YDNSRGEFLG PLFVEDKVNG 400
NSYYYPKGIY YVMDYFKTKY GDPLIYVTEN GFSTPSSENR EQAIADYKRI 450
DYLCSHLCFL RKVIKEKGVN VRGYFAWALG DNYEFCKGFT VRFGLSYVNW 500
EDLDDRNLKE SGKWYQRFIN GTVKNAVKQD FLRSSLSSQS QKKRFADA 548
Length:548
Mass (Da):62,736
Last modified:December 1, 1992 - v1
Checksum:iF3034C6A01CD732A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60214 mRNA. Translation: CAA42775.1.
PIRiS26149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60214 mRNA. Translation: CAA42775.1 .
PIRi S26149.

3D structure databases

ProteinModelPortali Q00326.
SMRi Q00326. Positions 23-520.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of a cDNA clone encoding the enzyme myrosinase, and expression of myrosinase in different tissues of Brassica napus."
    Falk A., Xue J., Lenman M., Rask L.
    Plant Sci. 83:181-186(1992)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Svalofs Karat.

Entry informationi

Entry nameiMYRO_BRANA
AccessioniPrimary (citable) accession number: Q00326
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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