Q00326 (MYRO_BRANA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Myrosinase EC=3.2.1.147 Alternative name(s): Sinigrinase Thioglucosidase
Organism	Brassica napus (Rape)
Taxonomic identifier	3708 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.
Catalytic activity	A thioglucoside + H2O = a sugar + a thiol.
Subunit structure	Homodimer.
Subcellular location	Vacuole.
Tissue specificity	In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant.
Miscellaneous	It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Cellular component	Vacuole
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thioglucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	By similarity
	Chain	21 – 548	528	Myrosinase		PRO_0000011774
Regions
	Region	484 – 485	2	Substrate binding By similarity
Sites
	Active site	429	1	Nucleophile By similarity
	Metal binding	76	1	Zinc; shared with dimeric partner By similarity
	Metal binding	90	1	Zinc; shared with dimeric partner By similarity
	Binding site	59	1	Substrate By similarity
	Binding site	161	1	Substrate By similarity
	Binding site	206	1	Substrate By similarity
	Binding site	207	1	Ascorbate By similarity
	Binding site	281	1	Ascorbate By similarity
	Binding site	352	1	Substrate By similarity
	Binding site	477	1	Substrate By similarity
Amino acid modifications
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
	Glycosylation	240	1	N-linked (GlcNAc...) Potential
	Glycosylation	331	1	N-linked (GlcNAc...) Potential
	Glycosylation	520	1	N-linked (GlcNAc...) Potential
	Disulfide bond	26 ↔ 458		By similarity
	Disulfide bond	34 ↔ 454		By similarity
	Disulfide bond	226 ↔ 236		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q00326 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: F3034C6A01CD732A	FASTA	548	62,736
	10 20 30 40 50 60 MKLLHGLALV FLLAAASCKA DEEITCEENN PFTCSNTDIL SSKNFGKDFI FGVASSAYQI 70 80 90 100 110 120 EGGRGRGVNV WDGFSHRYPE KAGSDLKNGD TTCESYTRWQ KDVDVMGELN ATGYRFSFAW 130 140 150 160 170 180 SRIIPKGKVS RGVNQGGLDY YHKLIDALLE KNITPFVTLF HWDLPQTLQD EYEGFLDRQI 190 200 210 220 230 240 IQDFKDYADL CFKEFGGKVK HWITINQLYT VPTRGYAIGT DAPGRCSPMV DTKHRCYGGN 250 260 270 280 290 300 SSTEPYIVAH NQLLAHATVV DLYRTKYKFQ KGKIGPVMIT RWFLPFDESD PASIEAAERM 310 320 330 340 350 360 NQFFHGWYME PLTKGRYPDI MRQIVGSRLP NFTEEEAELV AGSYDFLGLN YYVTQYAQPK 370 380 390 400 410 420 PNPYPSETHT AMMDAGVKLT YDNSRGEFLG PLFVEDKVNG NSYYYPKGIY YVMDYFKTKY 430 440 450 460 470 480 GDPLIYVTEN GFSTPSSENR EQAIADYKRI DYLCSHLCFL RKVIKEKGVN VRGYFAWALG 490 500 510 520 530 540 DNYEFCKGFT VRFGLSYVNW EDLDDRNLKE SGKWYQRFIN GTVKNAVKQD FLRSSLSSQS QKKRFADA

References

[1]	"Sequence of a cDNA clone encoding the enzyme myrosinase, and expression of myrosinase in different tissues of Brassica napus." Falk A., Xue J., Lenman M., Rask L. Plant Sci. 83:181-186(1992) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Svalofs Karat.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60214 mRNA. Translation: CAA42775.1.
PIR	S26149.
3D structure databases
ProteinModelPortal	Q00326.
SMR	Q00326. Positions 23-520.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10353. PTHR10353. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MYRO_BRANA

Accession

Primary (citable) accession number: Q00326

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	March 6, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program