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Protein

Myrosinase

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei59 – 591SubstrateBy similarity
Metal bindingi76 – 761Zinc; shared with dimeric partnerBy similarity
Metal bindingi90 – 901Zinc; shared with dimeric partnerBy similarity
Binding sitei161 – 1611SubstrateBy similarity
Binding sitei206 – 2061SubstrateBy similarity
Binding sitei207 – 2071AscorbateBy similarity
Binding sitei281 – 2811AscorbateBy similarity
Binding sitei352 – 3521SubstrateBy similarity
Active sitei429 – 4291NucleophilePROSITE-ProRule annotation
Binding sitei477 – 4771SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Myrosinase (EC:3.2.1.147)
Alternative name(s):
Sinigrinase
Thioglucosidase
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Chaini21 – 548528MyrosinasePRO_0000011774Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 458By similarity
Disulfide bondi34 ↔ 454By similarity
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi226 ↔ 236By similarity
Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi520 – 5201N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliQ00326.
SMRiQ00326. Positions 23-520.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni484 – 4852Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00326-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLHGLALV FLLAAASCKA DEEITCEENN PFTCSNTDIL SSKNFGKDFI
60 70 80 90 100
FGVASSAYQI EGGRGRGVNV WDGFSHRYPE KAGSDLKNGD TTCESYTRWQ
110 120 130 140 150
KDVDVMGELN ATGYRFSFAW SRIIPKGKVS RGVNQGGLDY YHKLIDALLE
160 170 180 190 200
KNITPFVTLF HWDLPQTLQD EYEGFLDRQI IQDFKDYADL CFKEFGGKVK
210 220 230 240 250
HWITINQLYT VPTRGYAIGT DAPGRCSPMV DTKHRCYGGN SSTEPYIVAH
260 270 280 290 300
NQLLAHATVV DLYRTKYKFQ KGKIGPVMIT RWFLPFDESD PASIEAAERM
310 320 330 340 350
NQFFHGWYME PLTKGRYPDI MRQIVGSRLP NFTEEEAELV AGSYDFLGLN
360 370 380 390 400
YYVTQYAQPK PNPYPSETHT AMMDAGVKLT YDNSRGEFLG PLFVEDKVNG
410 420 430 440 450
NSYYYPKGIY YVMDYFKTKY GDPLIYVTEN GFSTPSSENR EQAIADYKRI
460 470 480 490 500
DYLCSHLCFL RKVIKEKGVN VRGYFAWALG DNYEFCKGFT VRFGLSYVNW
510 520 530 540
EDLDDRNLKE SGKWYQRFIN GTVKNAVKQD FLRSSLSSQS QKKRFADA
Length:548
Mass (Da):62,736
Last modified:December 1, 1992 - v1
Checksum:iF3034C6A01CD732A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60214 mRNA. Translation: CAA42775.1.
PIRiS26149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60214 mRNA. Translation: CAA42775.1.
PIRiS26149.

3D structure databases

ProteinModelPortaliQ00326.
SMRiQ00326. Positions 23-520.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of a cDNA clone encoding the enzyme myrosinase, and expression of myrosinase in different tissues of Brassica napus."
    Falk A., Xue J., Lenman M., Rask L.
    Plant Sci. 83:181-186(1992)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Svalofs Karat.

Entry informationi

Entry nameiMYRO_BRANA
AccessioniPrimary (citable) accession number: Q00326
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: January 7, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates.

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.