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Q00313

- TOP1_CANAX

UniProt

Q00313 - TOP1_CANAX

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Protein

DNA topoisomerase 1

Gene
TOP1
Organism
Candida albicans (Yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei306 – 3061Interaction with DNA By similarity
Sitei354 – 3541Interaction with DNA By similarity
Sitei385 – 3851Interaction with DNA By similarity
Sitei442 – 4421Interaction with DNA By similarity
Sitei468 – 4681Interaction with DNA By similarity
Sitei511 – 5111Interaction with DNA By similarity
Sitei568 – 5681Interaction with DNA By similarity
Sitei586 – 5861Interaction with DNA By similarity
Active sitei736 – 7361O-(3'-phospho-DNA)-tyrosine intermediate

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-EC
  4. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Gene namesi
Name:TOP1
OrganismiCandida albicans (Yeast)
Taxonomic identifieri5476 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 778778DNA topoisomerase 1PRO_0000145208Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ00313.
SMRiQ00313. Positions 155-372.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni367 – 3682Interaction with DNA By similarity
Regioni430 – 4356Interaction with DNA By similarity
Regioni522 – 5243Interaction with DNA By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3569.

Family and domain databases

Gene3Di1.10.10.41. 1 hit.
1.10.132.10. 2 hits.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamiPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SMARTiSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00313-1 [UniParc]FASTAAdd to Basket

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MNSSDEEDIA LSRLAKKSSS ITSASTYEDD EDDDIPLAKK SRKKRVESDY    50
EEDEDEVPLK KLSNGRAKKQ VKTETKVKKE PKSANKSKST SKKDTKVKKE 100
KTTVKKESKA TSTKVKEESK TQSDSQASVK SETPEEDQGY KWWEVNQEEE 150
GDGYIKWQTL EHNGVMFPPP YEPLPSHVKL YYNNKPVNLP PEAEEVAGFY 200
GAMLETDHAK NPVFQKNFFN DFLEVLKECG GCGVEIKKFE KLDFSKMYAH 250
FEKLREEKKA MSREEKKRIK EEKEKEEEPY RTCYLNGRKE LVGNFRIEPP 300
GLFRGRGAHP KTGKLKRRVV SEQVTLNLGK DAKIPEPPAG HQWGEIRHDN 350
EVTWLAMWKE NISDSLKYVR FANNSSVKGQ SDFKKFETAR KLRDHVDSIR 400
KDYTKMLKSE KMQDRQMATA MYLIDVFALR AGGEKGEDEA DTVGCCSLRY 450
EHVTLKPPNK VIFDLLGKDS IRFYQEVEVD KQVFKNLRIF KKSPKQPGDD 500
LFDRINPSLV NRQLQNYMKG LTAKVFRTYN ASKTMQDQID IIENEGTVAE 550
KVAKFNAANR TVAILCNHQR TVSKTHGDSV QRINDKLKKF MWQKIRLKKM 600
ILQLEPKLKK KDSKYFEEID DLIKEDIEHI HHTIIKRQRE QAKKKLERDN 650
EKLKLEGKPL LTESDIKDKL DKIDELEKEY QKELKTGKPI VTKNATVEKL 700
KQQIETLENR ILNVSIQLKD KEDNSEVSLG TSKMNYIDPR LIVMFSKKFD 750
VPIEKLFTKT LREKFIWAIE SADENWRF 778
Length:778
Mass (Da):90,484
Last modified:November 1, 1997 - v1
Checksum:iBFABE6B22EA2E5D3
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21N → S in AAB39507. 1 Publication
Sequence conflicti61 – 611K → KRK1 Publication
Sequence conflicti465 – 4651L → F in AAB39507. 1 Publication
Sequence conflicti623 – 6231I → L in AAB39507. 1 Publication
Sequence conflicti710 – 7101R → K in AAB39507. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U40454 Genomic DNA. Translation: AAC49381.1.
U41342 Genomic DNA. Translation: AAB39507.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U40454 Genomic DNA. Translation: AAC49381.1 .
U41342 Genomic DNA. Translation: AAB39507.1 .

3D structure databases

ProteinModelPortali Q00313.
SMRi Q00313. Positions 155-372.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG3569.

Family and domain databases

Gene3Di 1.10.10.41. 1 hit.
1.10.132.10. 2 hits.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProi IPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view ]
Pfami PF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view ]
PRINTSi PR00416. EUTPISMRASEI.
SMARTi SM00435. TOPEUc. 1 hit.
[Graphical view ]
SUPFAMi SSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of the gene encoding DNA topoisomerase I from Candida albicans."
    Taylor A., Giles K., Sarthy A.V., McGonigal T., Fostel J.
    FEMS Microbiol. Lett. 138:113-121(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10321 / CCM 8215.
  2. "The topoisomerase I gene from Candida albicans."
    Jiang W., Gerhold D., Kmiec E.B., Hauser M., Becker J.M., Koltin Y.
    Microbiology 143:377-386(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiTOP1_CANAX
AccessioniPrimary (citable) accession number: Q00313
Secondary accession number(s): P78593
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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