Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q00313

- TOP1_CANAX

UniProt

Q00313 - TOP1_CANAX

Protein

DNA topoisomerase 1

Gene

TOP1

Organism
Candida albicans (Yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei306 – 3061Interaction with DNABy similarity
    Sitei354 – 3541Interaction with DNABy similarity
    Sitei385 – 3851Interaction with DNABy similarity
    Sitei442 – 4421Interaction with DNABy similarity
    Sitei468 – 4681Interaction with DNABy similarity
    Sitei511 – 5111Interaction with DNABy similarity
    Sitei568 – 5681Interaction with DNABy similarity
    Sitei586 – 5861Interaction with DNABy similarity
    Active sitei736 – 7361O-(3'-phospho-DNA)-tyrosine intermediate

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-EC
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

    GO - Biological processi

    1. DNA topological change Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1 (EC:5.99.1.2)
    Alternative name(s):
    DNA topoisomerase I
    Gene namesi
    Name:TOP1
    OrganismiCandida albicans (Yeast)
    Taxonomic identifieri5476 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 778778DNA topoisomerase 1PRO_0000145208Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ00313.
    SMRiQ00313. Positions 155-372.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni367 – 3682Interaction with DNABy similarity
    Regioni430 – 4356Interaction with DNABy similarity
    Regioni522 – 5243Interaction with DNABy similarity

    Sequence similaritiesi

    Belongs to the type IB topoisomerase family.Curated

    Phylogenomic databases

    eggNOGiCOG3569.

    Family and domain databases

    Gene3Di1.10.10.41. 1 hit.
    1.10.132.10. 2 hits.
    2.170.11.10. 2 hits.
    3.90.15.10. 1 hit.
    InterProiIPR011010. DNA_brk_join_enz.
    IPR013034. DNA_topo_domain1.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR025834. TopoI_C_dom.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR014727. TopoI_cat_a/b-sub_euk.
    IPR013500. TopoI_cat_euk.
    IPR008336. TopoI_DNA-bd_euk.
    IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
    IPR013499. TopoI_euk.
    [Graphical view]
    PfamiPF14370. Topo_C_assoc. 1 hit.
    PF01028. Topoisom_I. 1 hit.
    PF02919. Topoisom_I_N. 1 hit.
    [Graphical view]
    PRINTSiPR00416. EUTPISMRASEI.
    SMARTiSM00435. TOPEUc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56349. SSF56349. 2 hits.
    SSF56741. SSF56741. 1 hit.
    PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00313-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSSDEEDIA LSRLAKKSSS ITSASTYEDD EDDDIPLAKK SRKKRVESDY    50
    EEDEDEVPLK KLSNGRAKKQ VKTETKVKKE PKSANKSKST SKKDTKVKKE 100
    KTTVKKESKA TSTKVKEESK TQSDSQASVK SETPEEDQGY KWWEVNQEEE 150
    GDGYIKWQTL EHNGVMFPPP YEPLPSHVKL YYNNKPVNLP PEAEEVAGFY 200
    GAMLETDHAK NPVFQKNFFN DFLEVLKECG GCGVEIKKFE KLDFSKMYAH 250
    FEKLREEKKA MSREEKKRIK EEKEKEEEPY RTCYLNGRKE LVGNFRIEPP 300
    GLFRGRGAHP KTGKLKRRVV SEQVTLNLGK DAKIPEPPAG HQWGEIRHDN 350
    EVTWLAMWKE NISDSLKYVR FANNSSVKGQ SDFKKFETAR KLRDHVDSIR 400
    KDYTKMLKSE KMQDRQMATA MYLIDVFALR AGGEKGEDEA DTVGCCSLRY 450
    EHVTLKPPNK VIFDLLGKDS IRFYQEVEVD KQVFKNLRIF KKSPKQPGDD 500
    LFDRINPSLV NRQLQNYMKG LTAKVFRTYN ASKTMQDQID IIENEGTVAE 550
    KVAKFNAANR TVAILCNHQR TVSKTHGDSV QRINDKLKKF MWQKIRLKKM 600
    ILQLEPKLKK KDSKYFEEID DLIKEDIEHI HHTIIKRQRE QAKKKLERDN 650
    EKLKLEGKPL LTESDIKDKL DKIDELEKEY QKELKTGKPI VTKNATVEKL 700
    KQQIETLENR ILNVSIQLKD KEDNSEVSLG TSKMNYIDPR LIVMFSKKFD 750
    VPIEKLFTKT LREKFIWAIE SADENWRF 778
    Length:778
    Mass (Da):90,484
    Last modified:November 1, 1997 - v1
    Checksum:iBFABE6B22EA2E5D3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21N → S in AAB39507. (PubMed:9043115)Curated
    Sequence conflicti61 – 611K → KRK(PubMed:9043115)Curated
    Sequence conflicti465 – 4651L → F in AAB39507. (PubMed:9043115)Curated
    Sequence conflicti623 – 6231I → L in AAB39507. (PubMed:9043115)Curated
    Sequence conflicti710 – 7101R → K in AAB39507. (PubMed:9043115)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U40454 Genomic DNA. Translation: AAC49381.1.
    U41342 Genomic DNA. Translation: AAB39507.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U40454 Genomic DNA. Translation: AAC49381.1 .
    U41342 Genomic DNA. Translation: AAB39507.1 .

    3D structure databases

    ProteinModelPortali Q00313.
    SMRi Q00313. Positions 155-372.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG3569.

    Family and domain databases

    Gene3Di 1.10.10.41. 1 hit.
    1.10.132.10. 2 hits.
    2.170.11.10. 2 hits.
    3.90.15.10. 1 hit.
    InterProi IPR011010. DNA_brk_join_enz.
    IPR013034. DNA_topo_domain1.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR025834. TopoI_C_dom.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR014727. TopoI_cat_a/b-sub_euk.
    IPR013500. TopoI_cat_euk.
    IPR008336. TopoI_DNA-bd_euk.
    IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
    IPR013499. TopoI_euk.
    [Graphical view ]
    Pfami PF14370. Topo_C_assoc. 1 hit.
    PF01028. Topoisom_I. 1 hit.
    PF02919. Topoisom_I_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00416. EUTPISMRASEI.
    SMARTi SM00435. TOPEUc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56349. SSF56349. 2 hits.
    SSF56741. SSF56741. 1 hit.
    PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of the gene encoding DNA topoisomerase I from Candida albicans."
      Taylor A., Giles K., Sarthy A.V., McGonigal T., Fostel J.
      FEMS Microbiol. Lett. 138:113-121(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 10321 / CCM 8215.
    2. "The topoisomerase I gene from Candida albicans."
      Jiang W., Gerhold D., Kmiec E.B., Hauser M., Becker J.M., Koltin Y.
      Microbiology 143:377-386(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiTOP1_CANAX
    AccessioniPrimary (citable) accession number: Q00313
    Secondary accession number(s): P78593
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3