Reviewed, UniProtKB/Swiss-Prot Q00313 (TOP1_CANAL)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I

Gene names

Name:

TOP1

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	778 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Miscellaneous	Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils. When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the eukaryotic type I topoisomerase family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 778	778	DNA topoisomerase 1		PRO_0000145208
Sites
	Active site	736	1	O-(3'-phospho-DNA)-tyrosine intermediate
Experimental info
	Sequence conflict	2	1	N → S in AAB39507. Ref.2
	Sequence conflict	61	1	K → KRK Ref.2
	Sequence conflict	465	1	L → F in AAB39507. Ref.2
	Sequence conflict	623	1	I → L in AAB39507. Ref.2
	Sequence conflict	710	1	R → K in AAB39507. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q00313-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: BFABE6B22EA2E5D3	FASTA	778	90,484
	10 20 30 40 50 60 MNSSDEEDIA LSRLAKKSSS ITSASTYEDD EDDDIPLAKK SRKKRVESDY EEDEDEVPLK 70 80 90 100 110 120 KLSNGRAKKQ VKTETKVKKE PKSANKSKST SKKDTKVKKE KTTVKKESKA TSTKVKEESK 130 140 150 160 170 180 TQSDSQASVK SETPEEDQGY KWWEVNQEEE GDGYIKWQTL EHNGVMFPPP YEPLPSHVKL 190 200 210 220 230 240 YYNNKPVNLP PEAEEVAGFY GAMLETDHAK NPVFQKNFFN DFLEVLKECG GCGVEIKKFE 250 260 270 280 290 300 KLDFSKMYAH FEKLREEKKA MSREEKKRIK EEKEKEEEPY RTCYLNGRKE LVGNFRIEPP 310 320 330 340 350 360 GLFRGRGAHP KTGKLKRRVV SEQVTLNLGK DAKIPEPPAG HQWGEIRHDN EVTWLAMWKE 370 380 390 400 410 420 NISDSLKYVR FANNSSVKGQ SDFKKFETAR KLRDHVDSIR KDYTKMLKSE KMQDRQMATA 430 440 450 460 470 480 MYLIDVFALR AGGEKGEDEA DTVGCCSLRY EHVTLKPPNK VIFDLLGKDS IRFYQEVEVD 490 500 510 520 530 540 KQVFKNLRIF KKSPKQPGDD LFDRINPSLV NRQLQNYMKG LTAKVFRTYN ASKTMQDQID 550 560 570 580 590 600 IIENEGTVAE KVAKFNAANR TVAILCNHQR TVSKTHGDSV QRINDKLKKF MWQKIRLKKM 610 620 630 640 650 660 ILQLEPKLKK KDSKYFEEID DLIKEDIEHI HHTIIKRQRE QAKKKLERDN EKLKLEGKPL 670 680 690 700 710 720 LTESDIKDKL DKIDELEKEY QKELKTGKPI VTKNATVEKL KQQIETLENR ILNVSIQLKD 730 740 750 760 770 KEDNSEVSLG TSKMNYIDPR LIVMFSKKFD VPIEKLFTKT LREKFIWAIE SADENWRF

References

[1]	"Identification of the gene encoding DNA topoisomerase I from Candida albicans." Taylor A., Giles K., Sarthy A.V., McGonigal T., Fostel J. FEMS Microbiol. Lett. 138:113-121(1996) [PubMed: 9026437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10321 / CCM 8215.
[2]	"The topoisomerase I gene from Candida albicans." Jiang W., Gerhold D., Kmiec E.B., Hauser M., Becker J.M., Koltin Y. Microbiology 143:377-386(1997) [PubMed: 9043115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U40454 Genomic DNA. Translation: AAC49381.1. U41342 Genomic DNA. Translation: AAB39507.1.
3D structure databases
HSSP	HSSP built from PDB template 1OIS based on UniProtKB P04786.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.99.1.2. 1124.
Family and domain databases
InterPro	IPR018521. TopoI_AS. IPR001631. TopoI_C. IPR013499. TopoI_C_euk. IPR014711. TopoI_cat_a-hlx-sub_euk. IPR014727. TopoI_cat_a/b-sub_euk. IPR013500. TopoI_cat_euk. IPR013030. TopoI_DNA-bd_mixed-a/b_euk. IPR008336. TopoI_DNA_bd_euk. [Graphical view]
Gene3D	G3DSA:3.90.15.10. TopoI_cat_a-hlx-sub_euk. 1 hit. G3DSA:1.10.132.10. TopoI_cat_a/b-sub_euk. 1 hit. G3DSA:2.170.11.10. TopoI_DNA-bd_mixed-a/b_euk. 1 hit.
Pfam	PF01028. Topoisom_I. 1 hit. PF02919. Topoisom_I_N. 1 hit. [Graphical view]
PRINTS	PR00416. EUTPISMRASEI.
SMART	SM00435. TOPEUc. 1 hit. [Graphical view]
PROSITE	PS00176. TOPOISOMERASE_I_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_CANAL

Accession

Primary (citable) accession number: Q00313
Secondary accession number(s): P78593

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)