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Q00313 (TOP1_CANAX) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Gene names
Name:TOP1
OrganismCandida albicans (Yeast)
Taxonomic identifier5476 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length778 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Sequence similarities

Belongs to the type IB topoisomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 778778DNA topoisomerase 1
PRO_0000145208

Regions

Region367 – 3682Interaction with DNA By similarity
Region430 – 4356Interaction with DNA By similarity
Region522 – 5243Interaction with DNA By similarity

Sites

Active site7361O-(3'-phospho-DNA)-tyrosine intermediate
Site3061Interaction with DNA By similarity
Site3541Interaction with DNA By similarity
Site3851Interaction with DNA By similarity
Site4421Interaction with DNA By similarity
Site4681Interaction with DNA By similarity
Site5111Interaction with DNA By similarity
Site5681Interaction with DNA By similarity
Site5861Interaction with DNA By similarity

Experimental info

Sequence conflict21N → S in AAB39507. Ref.2
Sequence conflict611K → KRK Ref.2
Sequence conflict4651L → F in AAB39507. Ref.2
Sequence conflict6231I → L in AAB39507. Ref.2
Sequence conflict7101R → K in AAB39507. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q00313 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: BFABE6B22EA2E5D3

FASTA77890,484
        10         20         30         40         50         60 
MNSSDEEDIA LSRLAKKSSS ITSASTYEDD EDDDIPLAKK SRKKRVESDY EEDEDEVPLK 

        70         80         90        100        110        120 
KLSNGRAKKQ VKTETKVKKE PKSANKSKST SKKDTKVKKE KTTVKKESKA TSTKVKEESK 

       130        140        150        160        170        180 
TQSDSQASVK SETPEEDQGY KWWEVNQEEE GDGYIKWQTL EHNGVMFPPP YEPLPSHVKL 

       190        200        210        220        230        240 
YYNNKPVNLP PEAEEVAGFY GAMLETDHAK NPVFQKNFFN DFLEVLKECG GCGVEIKKFE 

       250        260        270        280        290        300 
KLDFSKMYAH FEKLREEKKA MSREEKKRIK EEKEKEEEPY RTCYLNGRKE LVGNFRIEPP 

       310        320        330        340        350        360 
GLFRGRGAHP KTGKLKRRVV SEQVTLNLGK DAKIPEPPAG HQWGEIRHDN EVTWLAMWKE 

       370        380        390        400        410        420 
NISDSLKYVR FANNSSVKGQ SDFKKFETAR KLRDHVDSIR KDYTKMLKSE KMQDRQMATA 

       430        440        450        460        470        480 
MYLIDVFALR AGGEKGEDEA DTVGCCSLRY EHVTLKPPNK VIFDLLGKDS IRFYQEVEVD 

       490        500        510        520        530        540 
KQVFKNLRIF KKSPKQPGDD LFDRINPSLV NRQLQNYMKG LTAKVFRTYN ASKTMQDQID 

       550        560        570        580        590        600 
IIENEGTVAE KVAKFNAANR TVAILCNHQR TVSKTHGDSV QRINDKLKKF MWQKIRLKKM 

       610        620        630        640        650        660 
ILQLEPKLKK KDSKYFEEID DLIKEDIEHI HHTIIKRQRE QAKKKLERDN EKLKLEGKPL 

       670        680        690        700        710        720 
LTESDIKDKL DKIDELEKEY QKELKTGKPI VTKNATVEKL KQQIETLENR ILNVSIQLKD 

       730        740        750        760        770 
KEDNSEVSLG TSKMNYIDPR LIVMFSKKFD VPIEKLFTKT LREKFIWAIE SADENWRF 

« Hide

References

[1]"Identification of the gene encoding DNA topoisomerase I from Candida albicans."
Taylor A., Giles K., Sarthy A.V., McGonigal T., Fostel J.
FEMS Microbiol. Lett. 138:113-121(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 10321 / CCM 8215.
[2]"The topoisomerase I gene from Candida albicans."
Jiang W., Gerhold D., Kmiec E.B., Hauser M., Becker J.M., Koltin Y.
Microbiology 143:377-386(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U40454 Genomic DNA. Translation: AAC49381.1.
U41342 Genomic DNA. Translation: AAB39507.1.

3D structure databases

ProteinModelPortalQ00313.
SMRQ00313. Positions 155-372.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG3569.

Family and domain databases

Gene3D1.10.10.41. 1 hit.
1.10.132.10. 2 hits.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSPR00416. EUTPISMRASEI.
SMARTSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_CANAX
AccessionPrimary (citable) accession number: Q00313
Secondary accession number(s): P78593
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families