Reviewed, UniProtKB/Swiss-Prot Q00310 (KRE2_CANAL)

Names and origin

Protein names

Recommended name:
    Glycolipid 2-alpha-mannosyltransferase
    EC=2.4.1.131
Alternative name(s):
    Alpha-1,2-mannosyltransferase

Gene names

Name:	KRE2
Synonyms:	MNT1
ORF Names:	CaO19.1665, CaO19.9234

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for the attachment of the third mannose residue of O-linked saccharides By similarity.
Catalytic activity	Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 15 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycolipid 2-alpha-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Glycolipid 2-alpha-mannosyltransferase		PRO_0000208240
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 28	19	Signal-anchor for type II membrane protein Potential
	Topological domain	29 – 431	403	Lumenal Potential
Sites
	Active site	318	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	Q00310-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: E8AC1FAE9010B763	FASTA	431	50,004
	10 20 30 40 50 60 MASTRSNARL IRFGIFALVL IGCGYILTRG SSFQPPNYQQ TQSPAAHEKQ TGNVAAGGGA 70 80 90 100 110 120 GSGSAGAQVP LGKNRGPIPK AIMGAGEGGS DAPVPQQDIP DSYTLNDKIK ATFVTLARNS 130 140 150 160 170 180 DLYSLAESIR HVEDRFNKKF HYDWVFLNDE EFNDEFKETV GSLVSGNTKF GLIPKEHWSY 190 200 210 220 230 240 PPWIDQEKAA LVREQMREKK IIYGHSESYR HMCRFESGFF WRQEILNDYD YYWRVEPDIK 250 260 270 280 290 300 LYCDIDYDIF KWMKDNNKDY AFTISLPEYK ETIPTLWDTT KEFIEKNPQY LAQNNLMDWV 310 320 330 340 350 360 SDDKGQTYNG CHFWSNFEIG SLAFWRSEAY RKYFEHLDKA GGFFYERWGD APVHSIAAAL 370 380 390 400 410 420 FLPREKIHFF EDVGYYHVPF TNCPVDKEVR KARNCNCDPN KDFTWRGYSC TTKYYTLNNF 430 KRQKGWEKYT A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of CaMnt1p, a mannosyl transferase important for adhesion and virulence of Candida albicans." Buurman E.T., Westwater C., Hube B., Brown A.J., Odds F.C., Gow N.A.R. Proc. Natl. Acad. Sci. U.S.A. 95:7670-7675(1998) [PubMed: 9636208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CAI4.
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X99619 Genomic DNA. Translation: CAA67930.1. AACQ01000014 Genomic DNA. Translation: EAL02827.1. AACQ01000013 Genomic DNA. Translation: EAL02954.1.
RefSeq	XP_721623.1. XP_721742.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT15. Glycosyltransferase Family 15.
Genome annotation databases
GeneID	3636585. 3636767.
Organism-specific databases
CGD	CAL0000071. MNT1.
Phylogenomic databases
OMA	Q00310. NGCHFWS.
Enzyme and pathway databases
BRENDA	2.4.1.131. 1124.
Family and domain databases
InterPro	IPR002685. Glyco_trans_15. [Graphical view]
Pfam	PF01793. Glyco_transf_15. 1 hit. [Graphical view]
PIRSF	PIRSF018153. Glyco_trans_15. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KRE2_CANAL

Accession

Primary (citable) accession number: Q00310
Secondary accession number(s): Q5AJC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)