Q00310 (KRE2_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycolipid 2-alpha-mannosyltransferase
EC=2.4.1.-

Alternative name(s):
Alpha-1,2-mannosyltransferase

Gene names

Name:	KRE2
Synonyms:	MNT1
ORF Names:	CaO19.1665, CaO19.9234

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for the attachment of the third mannose residue of O-linked saccharides By similarity.
Catalytic activity	Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 15 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cell-cell adhesion Inferred from mutant phenotype. Source: CGD cell-matrix adhesion Inferred from mutant phenotype. Source: CGD evasion or tolerance of defenses of other organism involved in symbiotic interaction Inferred from mutant phenotype. Source: CGD filamentous growth Inferred from genetic interaction. Source: CGD fungal-type cell wall organization Inferred from genetic interaction. Source: CGD mannoprotein biosynthetic process Inferred from mutant phenotype. Source: CGD pathogenesis Inferred from mutant phenotype Ref.1. Source: CGD protein O-linked glycosylation Inferred from mutant phenotype Ref.1. Source: CGD
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from direct assay. Source: CGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Inferred from direct assay. Source: CGD plasma membrane Inferred from direct assay. Source: CGD
Molecular function	alpha-1,2-mannosyltransferase activity Inferred from direct assay. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Glycolipid 2-alpha-mannosyltransferase		PRO_0000208240
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 28	19	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	29 – 431	403	Lumenal Potential
Sites
	Active site	318	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	Q00310 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: E8AC1FAE9010B763	FASTA	431	50,004
	10 20 30 40 50 60 MASTRSNARL IRFGIFALVL IGCGYILTRG SSFQPPNYQQ TQSPAAHEKQ TGNVAAGGGA 70 80 90 100 110 120 GSGSAGAQVP LGKNRGPIPK AIMGAGEGGS DAPVPQQDIP DSYTLNDKIK ATFVTLARNS 130 140 150 160 170 180 DLYSLAESIR HVEDRFNKKF HYDWVFLNDE EFNDEFKETV GSLVSGNTKF GLIPKEHWSY 190 200 210 220 230 240 PPWIDQEKAA LVREQMREKK IIYGHSESYR HMCRFESGFF WRQEILNDYD YYWRVEPDIK 250 260 270 280 290 300 LYCDIDYDIF KWMKDNNKDY AFTISLPEYK ETIPTLWDTT KEFIEKNPQY LAQNNLMDWV 310 320 330 340 350 360 SDDKGQTYNG CHFWSNFEIG SLAFWRSEAY RKYFEHLDKA GGFFYERWGD APVHSIAAAL 370 380 390 400 410 420 FLPREKIHFF EDVGYYHVPF TNCPVDKEVR KARNCNCDPN KDFTWRGYSC TTKYYTLNNF 430 KRQKGWEKYT A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of CaMnt1p, a mannosyl transferase important for adhesion and virulence of Candida albicans." Buurman E.T., Westwater C., Hube B., Brown A.J., Odds F.C., Gow N.A.R. Proc. Natl. Acad. Sci. U.S.A. 95:7670-7675(1998) [PubMed: 9636208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CAI4.
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X99619 Genomic DNA. Translation: CAA67930.1. AACQ01000014 Genomic DNA. Translation: EAL02827.1. AACQ01000013 Genomic DNA. Translation: EAL02954.1.
RefSeq	XP_721623.1. XM_716530.1. XP_721742.1. XM_716649.1.
3D structure databases
ProteinModelPortal	Q00310.
SMR	Q00310. Positions 101-429.
ModBase	Search...
Protein family/group databases
CAZy	GT15. Glycosyltransferase Family 15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3636585. 3636767.
KEGG	cal:CaO19.1665. cal:CaO19.9234.
Organism-specific databases
CGD	CAL0000071. MNT1.
Phylogenomic databases
OMA	EYKETIP.
PhylomeDB	Q00310.
Family and domain databases
InterPro	IPR002685. Glyco_trans_15. [Graphical view]
KO	K10967.
Pfam	PF01793. Glyco_transf_15. 1 hit. [Graphical view]
PIRSF	PIRSF018153. Glyco_trans_15. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KRE2_CANAL

Accession

Primary (citable) accession number: Q00310
Secondary accession number(s): Q5AJC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program