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Protein

Nitrate reductase

Gene

NiaD

Organism
Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADSAAS annotation
  • Mo-molybdopterinUniRule annotationNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi170MolybdenumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseSAAS annotation
Biological processNitrate assimilationUniRule annotation
LigandFADSAAS annotation, Flavoprotein, HemeSAAS annotation, Iron, Metal-binding, MolybdenumUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductaseUniRule annotation
Gene namesi
Name:NiaDImported
OrganismiBotryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea)Imported
Taxonomic identifieri40559 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeBotrytis

Interactioni

Subunit structurei

Homodimer.SAAS annotation

Structurei

3D structure databases

ProteinModelPortaliQ00303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini538 – 612Cytochrome b5 heme-bindingInterPro annotationAdd BLAST75
Domaini640 – 751FAD-binding FR-typeInterPro annotationAdd BLAST112

Sequence similaritiesi

Belongs to the nitrate reductase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiView protein in InterPro
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
PfamiView protein in Pfam
PF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
SMARTiView protein in SMART
SM01117. Cyt-b5. 1 hit.
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiView protein in PROSITE
PS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.

Sequencei

Sequence statusi: Complete.

Q00303-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASAQFMNEH SSEADAEMSK MIPQLPQTPP DSNENSSDEL GSLSSYSSST
60 70 80 90 100
SLSLQRYTLP PPTNPPTEIL KEDLKTPDNQ VPRDPRLIRL TGIHPFNVEP
110 120 130 140 150
PLSALYDEGF LTSPELFYVR NHGAVPHVED SSIPDWEFSV EGLVKKPFTL
160 170 180 190 200
TLKDLLREYE QITVPITLVC AGNRRKEQNQ VRKSKGFSWG AAGVSTALWT
210 220 230 240 250
GVPMYELIRK AMPMRGAKYV CMEGADKLPN GYYGTSLKLN WVMDPNRGIM
260 270 280 290 300
VAHKMNGEML RPDHGKPLRA VVPGQIGGRS VKWLKKIIVT AAPSDNWYHI
310 320 330 340 350
YDNRVLPTMI SPEQSANEPK WWTDERYAIY DLSTNSATAY PEHEERLSLV
360 370 380 390 400
SGQQTYRAKG YAYGGGGRRV TRVEVTLDKG KSWALLDIRY HEDDYRDRAY
410 420 430 440 450
ENETLFGGKL DMEWRETCFT WCFWEVDLDV QDLAMSGDIM VRAMDESMNV
460 470 480 490 500
QPRDMYWSVL GMMNNPWYRV TITKEGDYLR FEHPTQPALM PGGWMERVKS
510 520 530 540 550
AGGNLANGFW GEKMGTEDES VALVEVKQEI KMTKDDVKRE ISIEELRQHD
560 570 580 590 600
NEKEPWFVLN GEVYDGAPFL EGHPGGATSI TGAAEDSTDE FMAIHSETAK
610 620 630 640 650
AMMPAYHIGT LSPSAFTSLL NPEPLSSSSQ SLRPIFLQPK SWQKAVLTAK
660 670 680 690 700
KVISSDTRIF SFTLDHAEQV IGLPVGQHLM MRLRDPVTRE AIIRSYTPLS
710 720 730 740 750
LGTDKGVLDI LIKIYLKNEK GPGGKMTMAL ESIPVGHSID FKGPIGKFEY
760 770 780 790 800
VAKGECVIGG KKRRVKRFVM ICGGSGVTPI FSVLRAVLNE EEVGGAPKCI
810 820 830 840 850
VLDGNRREED ILCREELDDL VKGNEQRCRL VHALSQPTEE WKGLRGRLGK
860 870 880 890 900
EVLEKEVGRF EMDMDGEGNG YRDGEQLVLI CGPEALEKNV HGILSDLGWK

DQDILFF
Length:907
Mass (Da):101,897
Last modified:November 1, 1996 - v1
Checksum:i9E04BA54B5084FAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43783 Genomic DNA. Translation: AAC02633.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiQ00303_BOTFU
AccessioniPrimary (citable) accession number: Q00303
Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: July 5, 2017
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)