ID   PAX8_MOUSE              Reviewed;         457 AA.
AC   Q00288; P97342; Q6GU20;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 3.
DT   27-MAR-2024, entry version 173.
DE   RecName: Full=Paired box protein Pax-8;
GN   Name=Pax8; Synonyms=Pax-8;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1723950; DOI=10.1242/dev.110.2.643;
RA   Plachov D., Chowdhury K., Walther C., Simon D., Guenet J.-L., Gruss P.;
RT   "Pax8, a murine paired box gene expressed in the developing excretory
RT   system and thyroid gland.";
RL   Development 110:643-651(1990).
RN   [2]
RP   SEQUENCE REVISION TO 158; 263 AND 340.
RX   PubMed=1516825; DOI=10.1101/gad.6.9.1589;
RA   Adams B., Doerfler P., Aguzzi A., Kozmik Z., Urbanek P., Maurer-Fogy I.,
RA   Busslinger M.;
RT   "Pax-5 encodes the transcription factor BSAP and is expressed in B
RT   lymphocytes, the developing CNS, and adult testis.";
RL   Genes Dev. 6:1589-1607(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=C57BL/6J; TISSUE=Kidney;
RX   PubMed=9205117; DOI=10.1006/geno.1997.4735;
RA   Okladnova O., Poleev A., Fantes J., Lee M., Plachov D., Horst J.;
RT   "The genomic organization of the murine Pax 8 gene and characterization of
RT   its basal promoter.";
RL   Genomics 42:452-461(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Kidney;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Thought to encode a transcription factor. It may have a role
CC       in kidney cell differentiation. May play a regulatory role in mammalian
CC       development.
CC   -!- SUBUNIT: Interacts with WWTR1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Expressed in the developing excretory system and
CC       the thyroid gland.
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DR   EMBL; X57487; CAA40725.1; -; mRNA.
DR   EMBL; X99592; CAA67904.1; -; Genomic_DNA.
DR   EMBL; X99593; CAA67904.1; JOINED; Genomic_DNA.
DR   EMBL; X99594; CAA67904.1; JOINED; Genomic_DNA.
DR   EMBL; X99595; CAA67904.1; JOINED; Genomic_DNA.
DR   EMBL; X99596; CAA67904.1; JOINED; Genomic_DNA.
DR   EMBL; X99597; CAA67904.1; JOINED; Genomic_DNA.
DR   EMBL; X99598; CAA67904.1; JOINED; Genomic_DNA.
DR   EMBL; AL732528; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH466542; EDL08172.1; -; Genomic_DNA.
DR   EMBL; BC020526; AAH20526.1; -; mRNA.
DR   CCDS; CCDS15738.1; -.
DR   PIR; A56925; A56925.
DR   RefSeq; NP_035170.1; NM_011040.4.
DR   AlphaFoldDB; Q00288; -.
DR   BMRB; Q00288; -.
DR   SMR; Q00288; -.
DR   BioGRID; 202035; 4.
DR   IntAct; Q00288; 3.
DR   STRING; 10090.ENSMUSP00000028355; -.
DR   iPTMnet; Q00288; -.
DR   PhosphoSitePlus; Q00288; -.
DR   MaxQB; Q00288; -.
DR   PaxDb; 10090-ENSMUSP00000028355; -.
DR   ProteomicsDB; 294390; -.
DR   Antibodypedia; 9626; 1052 antibodies from 48 providers.
DR   DNASU; 18510; -.
DR   Ensembl; ENSMUST00000028355.11; ENSMUSP00000028355.5; ENSMUSG00000026976.16.
DR   GeneID; 18510; -.
DR   KEGG; mmu:18510; -.
DR   UCSC; uc008ipb.2; mouse.
DR   AGR; MGI:97492; -.
DR   CTD; 7849; -.
DR   MGI; MGI:97492; Pax8.
DR   VEuPathDB; HostDB:ENSMUSG00000026976; -.
DR   eggNOG; KOG3862; Eukaryota.
DR   GeneTree; ENSGT00940000161868; -.
DR   InParanoid; Q00288; -.
DR   OMA; CNISCYA; -.
DR   OrthoDB; 5393942at2759; -.
DR   PhylomeDB; Q00288; -.
DR   TreeFam; TF315397; -.
DR   BioGRID-ORCS; 18510; 9 hits in 79 CRISPR screens.
DR   ChiTaRS; Pax8; mouse.
DR   PRO; PR:Q00288; -.
DR   Proteomes; UP000000589; Chromosome 2.
DR   RNAct; Q00288; Protein.
DR   Bgee; ENSMUSG00000026976; Expressed in thyroid gland and 96 other cell types or tissues.
DR   ExpressionAtlas; Q00288; baseline and differential.
DR   GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0005667; C:transcription regulator complex; TAS:MGI.
DR   GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR   GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR   GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR   GO; GO:0009887; P:animal organ morphogenesis; TAS:MGI.
DR   GO; GO:0001658; P:branching involved in ureteric bud morphogenesis; IEA:Ensembl.
DR   GO; GO:0071371; P:cellular response to gonadotropin stimulus; ISO:MGI.
DR   GO; GO:0007417; P:central nervous system development; IEP:UniProtKB.
DR   GO; GO:0006351; P:DNA-templated transcription; ISO:MGI.
DR   GO; GO:0030855; P:epithelial cell differentiation; IMP:MGI.
DR   GO; GO:0042472; P:inner ear morphogenesis; IGI:UniProtKB.
DR   GO; GO:0072073; P:kidney epithelium development; IEP:UniProtKB.
DR   GO; GO:0003337; P:mesenchymal to epithelial transition involved in metanephros morphogenesis; IEA:Ensembl.
DR   GO; GO:0072164; P:mesonephric tubule development; IEP:UniProtKB.
DR   GO; GO:0001823; P:mesonephros development; IMP:UniProtKB.
DR   GO; GO:0072278; P:metanephric comma-shaped body morphogenesis; IEA:Ensembl.
DR   GO; GO:0072221; P:metanephric distal convoluted tubule development; IMP:UniProtKB.
DR   GO; GO:0072289; P:metanephric nephron tubule formation; IMP:UniProtKB.
DR   GO; GO:0072284; P:metanephric S-shaped body morphogenesis; IEA:Ensembl.
DR   GO; GO:0001656; P:metanephros development; IGI:MGI.
DR   GO; GO:1900215; P:negative regulation of apoptotic process involved in metanephric collecting duct development; IMP:UniProtKB.
DR   GO; GO:1900218; P:negative regulation of apoptotic process involved in metanephric nephron tubule development; IMP:UniProtKB.
DR   GO; GO:0010667; P:negative regulation of cardiac muscle cell apoptotic process; IMP:BHF-UCL.
DR   GO; GO:0072305; P:negative regulation of mesenchymal cell apoptotic process involved in metanephric nephron morphogenesis; IMP:UniProtKB.
DR   GO; GO:1900212; P:negative regulation of mesenchymal cell apoptotic process involved in metanephros development; IMP:UniProtKB.
DR   GO; GO:0071599; P:otic vesicle development; IEA:Ensembl.
DR   GO; GO:0090190; P:positive regulation of branching involved in ureteric bud morphogenesis; IMP:UniProtKB.
DR   GO; GO:0045893; P:positive regulation of DNA-templated transcription; IDA:UniProtKB.
DR   GO; GO:0072108; P:positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis; IMP:UniProtKB.
DR   GO; GO:2000594; P:positive regulation of metanephric DCT cell differentiation; IMP:UniProtKB.
DR   GO; GO:2000611; P:positive regulation of thyroid hormone generation; IMP:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR   GO; GO:0039003; P:pronephric field specification; IEP:UniProtKB.
DR   GO; GO:0048793; P:pronephros development; IMP:UniProtKB.
DR   GO; GO:0042981; P:regulation of apoptotic process; IMP:UniProtKB.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; ISO:MGI.
DR   GO; GO:0072307; P:regulation of metanephric nephron tubule epithelial cell differentiation; IMP:UniProtKB.
DR   GO; GO:2000612; P:regulation of thyroid-stimulating hormone secretion; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0072050; P:S-shaped body morphogenesis; IEP:UniProtKB.
DR   GO; GO:0006790; P:sulfur compound metabolic process; IEA:Ensembl.
DR   GO; GO:0030878; P:thyroid gland development; IMP:MGI.
DR   GO; GO:0001655; P:urogenital system development; IGI:UniProtKB.
DR   GO; GO:0003281; P:ventricular septum development; IMP:BHF-UCL.
DR   CDD; cd00131; PAX; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 2.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR043182; PAIRED_DNA-bd_dom.
DR   InterPro; IPR001523; Paired_dom.
DR   InterPro; IPR022130; Pax2_C.
DR   InterPro; IPR043565; PAX_fam.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR45636; PAIRED BOX PROTEIN PAX-6-RELATED-RELATED; 1.
DR   PANTHER; PTHR45636:SF6; PAIRED BOX PROTEIN PAX-8; 1.
DR   Pfam; PF00292; PAX; 1.
DR   Pfam; PF12403; Pax2_C; 1.
DR   PRINTS; PR00027; PAIREDBOX.
DR   SMART; SM00351; PAX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00034; PAIRED_1; 1.
DR   PROSITE; PS51057; PAIRED_2; 1.
DR   Genevisible; Q00288; MM.
PE   1: Evidence at protein level;
KW   Developmental protein; Differentiation; DNA-binding; Nucleus; Paired box;
KW   Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..457
FT                   /note="Paired box protein Pax-8"
FT                   /id="PRO_0000050198"
FT   DNA_BIND        9..135
FT                   /note="Paired"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT   REGION          12..68
FT                   /note="PAI subdomain"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT   REGION          87..135
FT                   /note="RED subdomain"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT   REGION          159..224
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        159..188
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        208..224
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         304
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   CONFLICT        340
FT                   /note="G -> D (in Ref. 2; no nucleotide entry)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   457 AA;  48793 MW;  AAACD3EE54AA4488 CRC64;
     MPHNSIRSGH GGLNQLGGAF VNGRPLPEVV RQRIVDLAHQ GVRPCDISRQ LRVSHGCVSK
     ILGRYYETGS IRPGVIGGSK PKVATPKVVE KIGDYKRQNP TMFAWEIRDR LLAEGVCDND
     TVPSVSSINR IIRTKVQQPF NLPMDSCVAT KSLSPGHTLI PSSAVTPPES PQSDSLGSTY
     SINGLLGIAQ PGNDNKRKMD DSDQDSCRLS IDSQSSSSGP RKHLRTDTFS QHHLEALECP
     FERQHYPEAY ASPSHTKGEQ GLYPLPLLNS ALDDGKATLT SSNTPLGRNL STHQTYPVVA
     DPHSPFAIKQ ETPELSSSSS TPSSLSSSAF LDLQQVGSGG PAGASVPPFN AFPHAASVYG
     QFTGQALLSG REMVGPTLPG YPPHIPTSGQ GSYASSAIAG MVAGSEYSGN AYSHTPYSSY
     SEAWRFPNSS LLSSPYYYSS TSRPSAPPTS ATAFDHL
//