Q00288 (PAX8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Paired box protein Pax-8

Gene names

Name:	Pax8
Synonyms:	Pax-8

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Thought to encode a transcription factor. It may have a role in kidney cell differentiation. May play a regulatory role in mammalian development.
Subunit structure	Interacts with WWTR1 By similarity.
Subcellular location	Nucleus.
Tissue specificity	Expressed in the developing excretory system and the thyroid gland.
Sequence similarities	Contains 1 paired domain.

Ontologies

Keywords
Biological process	Differentiation Transcription Transcription regulation
Cellular component	Nucleus
Domain	Paired box
Ligand	DNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	S-shaped body morphogenesis Inferred from expression pattern Ref.1. Source: UniProtKB branching involved in ureteric bud morphogenesis Inferred from electronic annotation. Source: Compara cell differentiation Inferred from mutant phenotype PubMed 9590297. Source: MGI cellular response to gonadotropin stimulus Inferred from electronic annotation. Source: Compara central nervous system development Inferred from expression pattern Ref.1. Source: UniProtKB inner ear morphogenesis Inferred from genetic interaction PubMed 20727173. Source: UniProtKB mesenchymal to epithelial transition involved in metanephros morphogenesis Inferred from electronic annotation. Source: Compara mesonephric tubule development Inferred from expression pattern Ref.1. Source: UniProtKB metanephric S-shaped body morphogenesis Inferred from electronic annotation. Source: Compara metanephric comma-shaped body morphogenesis Inferred from electronic annotation. Source: Compara metanephric distal convoluted tubule development Inferred from mutant phenotype PubMed 17314325. Source: UniProtKB metanephric nephron tubule formation Inferred from mutant phenotype PubMed 17314325. Source: UniProtKB negative regulation of apoptotic process involved in metanephric collecting duct development Inferred from mutant phenotype PubMed 17314325. Source: UniProtKB negative regulation of apoptotic process involved in metanephric nephron tubule development Inferred from mutant phenotype PubMed 17314325. Source: UniProtKB negative regulation of mesenchymal cell apoptotic process involved in metanephric nephron morphogenesis Inferred from mutant phenotype PubMed 17314325. Source: UniProtKB otic vesicle development Inferred from electronic annotation. Source: Compara positive regulation of branching involved in ureteric bud morphogenesis Inferred from mutant phenotype PubMed 17314325. Source: UniProtKB positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis Inferred from mutant phenotype PubMed 12435636. Source: UniProtKB positive regulation of metanephric DCT cell differentiation Inferred from mutant phenotype PubMed 17314325. Source: UniProtKB positive regulation of thyroid hormone generation Inferred from mutant phenotype PubMed 12586753. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from sequence orthology PubMed 9214635. Source: MGI pronephric field specification Inferred from expression pattern PubMed 12435636. Source: UniProtKB regulation of metanephric nephron tubule epithelial cell differentiation Inferred from mutant phenotype PubMed 17314325. Source: UniProtKB regulation of thyroid-stimulating hormone secretion Inferred from sequence or structural similarity. Source: UniProtKB thyroid gland development Inferred from expression pattern Ref.1. Source: UniProtKB transcription, DNA-dependent Inferred from direct assay PubMed 8413205. Source: UniProtKB
Cellular_component	transcription factor complex Traceable author statement PubMed 10197584. Source: MGI
Molecular_function	RNA polymerase II core promoter sequence-specific DNA binding Inferred from sequence or structural similarity. Source: UniProtKB sequence-specific DNA binding transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Paired box protein Pax-8		PRO_0000050198
Regions
	Domain	9 – 135	127	Paired
	Compositional bias	316 – 328	13	Ser-rich
Amino acid modifications
	Modified residue	280	1	Phosphothreonine By similarity
	Modified residue	284	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	340	1	G → D no nucleotide entry Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q00288 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: AAACD3EE54AA4488	FASTA	457	48,793
	10 20 30 40 50 60 MPHNSIRSGH GGLNQLGGAF VNGRPLPEVV RQRIVDLAHQ GVRPCDISRQ LRVSHGCVSK 70 80 90 100 110 120 ILGRYYETGS IRPGVIGGSK PKVATPKVVE KIGDYKRQNP TMFAWEIRDR LLAEGVCDND 130 140 150 160 170 180 TVPSVSSINR IIRTKVQQPF NLPMDSCVAT KSLSPGHTLI PSSAVTPPES PQSDSLGSTY 190 200 210 220 230 240 SINGLLGIAQ PGNDNKRKMD DSDQDSCRLS IDSQSSSSGP RKHLRTDTFS QHHLEALECP 250 260 270 280 290 300 FERQHYPEAY ASPSHTKGEQ GLYPLPLLNS ALDDGKATLT SSNTPLGRNL STHQTYPVVA 310 320 330 340 350 360 DPHSPFAIKQ ETPELSSSSS TPSSLSSSAF LDLQQVGSGG PAGASVPPFN AFPHAASVYG 370 380 390 400 410 420 QFTGQALLSG REMVGPTLPG YPPHIPTSGQ GSYASSAIAG MVAGSEYSGN AYSHTPYSSY 430 440 450 SEAWRFPNSS LLSSPYYYSS TSRPSAPPTS ATAFDHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pax8, a murine paired box gene expressed in the developing excretory system and thyroid gland." Plachov D., Chowdhury K., Walther C., Simon D., Guenet J.-L., Gruss P. Development 110:643-651(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Pax-5 encodes the transcription factor BSAP and is expressed in B lymphocytes, the developing CNS, and adult testis." Adams B., Doerfler P., Aguzzi A., Kozmik Z., Urbanek P., Maurer-Fogy I., Busslinger M. Genes Dev. 6:1589-1607(1992) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 158; 263 AND 340.
[3]	"The genomic organization of the murine Pax 8 gene and characterization of its basal promoter." Okladnova O., Poleev A., Fantes J., Lee M., Plachov D., Horst J. Genomics 42:452-461(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6. Tissue: Kidney.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57487 mRNA. Translation: CAA40725.1. X99592 , X99593, X99594, X99595, X99596, X99597, X99598 Genomic DNA. Translation: CAA67904.1. AL732528 Genomic DNA. Translation: CAM17808.1. CH466542 Genomic DNA. Translation: EDL08172.1. BC020526 mRNA. Translation: AAH20526.1.
IPI	IPI00309457.
PIR	A56925.
RefSeq	NP_035170.1. NM_011040.3.
UniGene	Mm.2533.
3D structure databases
ProteinModelPortal	Q00288.
SMR	Q00288. Positions 1-146, 222-276.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q00288. 1 interaction.
PTM databases
PhosphoSite	Q00288.
Proteomic databases
PaxDb	Q00288.
PRIDE	Q00288.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000028355; ENSMUSP00000028355; ENSMUSG00000026976.
GeneID	18510.
KEGG	mmu:18510.
Organism-specific databases
CTD	7849.
MGI	MGI:97492. Pax8.
Phylogenomic databases
eggNOG	NOG326044.
GeneTree	ENSGT00680000099910.
HOGENOM	HOG000230938.
HOVERGEN	HBG009115.
InParanoid	Q6GU20.
KO	K09293.
OMA	EQGLYPL.
OrthoDB	EOG42NJ0G.
Gene expression databases
ArrayExpress	Q00288.
Bgee	Q00288.
CleanEx	MM_PAX8.
Genevestigator	Q00288.
GermOnline	ENSMUSG00000026976. Mus musculus.
Family and domain databases
Gene3D	1.10.10.10. 2 hits.
InterPro	IPR009057. Homeodomain-like. IPR001523. Paired_dom. IPR022130. Pax2_C. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00292. PAX. 1 hit. PF12403. Pax2_C. 1 hit. [Graphical view]
PRINTS	PR00027. PAIREDBOX.
SMART	SM00351. PAX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00034. PAIRED_1. 1 hit. PS51057. PAIRED_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	294244.
SOURCE	Search...

Entry information

Entry name

PAX8_MOUSE

Accession

Primary (citable) accession number: Q00288
Secondary accession number(s): P97342, Q6GU20

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 106 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program