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Protein

Pituitary-specific positive transcription factor 1

Gene

Pou1f1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi214 – 273HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

  • B cell differentiation Source: MGI
  • cell fate specification Source: MGI
  • determination of adult lifespan Source: MGI
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • nuclear transport Source: Ensembl
  • pituitary gland development Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of inositol trisphosphate biosynthetic process Source: MGI
  • positive regulation of multicellular organism growth Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of insulin-like growth factor receptor signaling pathway Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • somatotropin secreting cell development Source: MGI
  • somatotropin secreting cell differentiation Source: MGI

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary-specific positive transcription factor 1
Short name:
PIT-1
Alternative name(s):
Growth hormone factor 1
Short name:
GHF-1
Gene namesi
Name:Pou1f1
Synonyms:Pit-1, Pit1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:97588 Pou1f1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Pou1f1 are the cause of the dwarf (dw) phenotype which interrupts the normal development of the anterior pituitary gland, resulting in the loss of expression of growth hormone, prolactin and thyroid-stimulating hormone, and hypoplasia of their respective cell types.1 Publication

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001006991 – 291Pituitary-specific positive transcription factor 1Add BLAST291

Proteomic databases

PaxDbiQ00286
PRIDEiQ00286

PTM databases

PhosphoSitePlusiQ00286

Expressioni

Gene expression databases

BgeeiENSMUSG00000004842
ExpressionAtlasiQ00286 baseline and differential
GenevisibleiQ00286 MM

Interactioni

Subunit structurei

Interacts with PITX1. Interacts with LHX3. Interacts with ELK1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-60278N
IntActiQ00286, 1 interactor
STRINGi10090.ENSMUSP00000135113

Structurei

3D structure databases

ProteinModelPortaliQ00286
SMRiQ00286
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini124 – 198POU-specificPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802 Eukaryota
ENOG410XQ7X LUCA
GeneTreeiENSGT00760000118935
HOGENOMiHOG000116302
HOVERGENiHBG053120
InParanoidiQ00286
KOiK09363
PhylomeDBiQ00286

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR015586 Pit_1
IPR013847 POU
IPR000327 POU_dom
PANTHERiPTHR11636:SF84 PTHR11636:SF84, 2 hits
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00157 Pou, 1 hit
PRINTSiPR00028 POUDOMAIN
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00352 POU, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00035 POU_1, 1 hit
PS00465 POU_2, 1 hit
PS51179 POU_3, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q00286-1) [UniParc]FASTAAdd to basket
Also known as: Pit-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSCQSFTSAD TFITLNSDAS AALPLRMHHS AAECLPASNH ATNVMSTATG
60 70 80 90 100
LHYSVPSCHY GNQPSTYGVM AGSLTPCLYK FPDHTLSHGF PPLHQPLLAE
110 120 130 140 150
DPAASEFKQE LRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKLGYTQ
160 170 180 190 200
TNVGEALAAV HGSEFSQTTI CRFENLQLSF KNACKLKAIL SKWLEEAEQV
210 220 230 240 250
GALYNEKVGA NERKRKRRTT ISVAAKDALE RHFGEHSKPS SQEIMRMAEE
260 270 280 290
LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R
Length:291
Mass (Da):32,885
Last modified:December 1, 1992 - v1
Checksum:iE2C265F4353E84C5
GO
Isoform 2 (identifier: Q00286-2) [UniParc]FASTAAdd to basket
Also known as: Pit-1T

The sequence of this isoform differs from the canonical sequence as follows:
     48-48: A → GLHTYFSMTTMGNTA

Show »
Length:305
Mass (Da):34,428
Checksum:i965F1066F30DDE0B
GO
Isoform 3 (identifier: Q00286-3) [UniParc]FASTAAdd to basket
Also known as: Pit-1a, Pit-1 beta

The sequence of this isoform differs from the canonical sequence as follows:
     48-48: A → VPSILSLIQTPKCLHTYFSMTTMGNTA

Note: Unable to transactivate. Only about 1/7 as abundant as isoform 1.
Show »
Length:317
Mass (Da):35,752
Checksum:i5AE06A891EFBB18B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti261W → C in dw. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01255048A → GLHTYFSMTTMGNTA in isoform 2. Curated1
Alternative sequenceiVSP_01255148A → VPSILSLIQTPKCLHTYFSM TTMGNTA in isoform 3. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12885 mRNA Translation: BAA02289.1
X57512 mRNA Translation: CAA40737.1
BC061213 mRNA Translation: AAH61213.1
CCDSiCCDS57033.1 [Q00286-1]
PIRiS11663
RefSeqiNP_032875.1, NM_008849.4 [Q00286-1]
UniGeneiMm.355440

Genome annotation databases

EnsembliENSMUST00000176330; ENSMUSP00000135113; ENSMUSG00000004842 [Q00286-1]
GeneIDi18736
KEGGimmu:18736
UCSCiuc007zqi.1 mouse [Q00286-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPIT1_MOUSE
AccessioniPrimary (citable) accession number: Q00286
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: March 28, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health