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Q00286

- PIT1_MOUSE

UniProt

Q00286 - PIT1_MOUSE

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Protein
Pituitary-specific positive transcription factor 1
Gene
Pou1f1, Pit-1, Pit1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi214 – 27360Homeobox
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
  3. RNA polymerase II transcription coactivator activity Source: Ensembl
  4. chromatin binding Source: MGI
  5. protein binding Source: MGI
  6. sequence-specific DNA binding Source: MGI
  7. sequence-specific DNA binding transcription factor activity Source: MGI
  8. transcription corepressor activity Source: Ensembl
  9. transcription factor binding Source: MGI

GO - Biological processi

  1. B cell differentiation Source: MGI
  2. cell fate specification Source: MGI
  3. determination of adult lifespan Source: MGI
  4. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  5. nuclear transport Source: Ensembl
  6. pituitary gland development Source: MGI
  7. positive regulation of cell proliferation Source: MGI
  8. positive regulation of inositol trisphosphate biosynthetic process Source: MGI
  9. positive regulation of multicellular organism growth Source: MGI
  10. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  11. positive regulation of transcription, DNA-templated Source: MGI
  12. regulation of insulin-like growth factor receptor signaling pathway Source: MGI
  13. somatotropin secreting cell development Source: MGI
  14. somatotropin secreting cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary-specific positive transcription factor 1
Short name:
PIT-1
Alternative name(s):
Growth hormone factor 1
Short name:
GHF-1
Gene namesi
Name:Pou1f1
Synonyms:Pit-1, Pit1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:97588. Pou1f1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: MGI
  3. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Pou1f1 are the cause of the dwarf (dw) phenotype which interrupts the normal development of the anterior pituitary gland, resulting in the loss of expression of growth hormone, prolactin and thyroid-stimulating hormone, and hypoplasia of their respective cell types.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Pituitary-specific positive transcription factor 1
PRO_0000100699Add
BLAST

Proteomic databases

PRIDEiQ00286.

PTM databases

PhosphoSiteiQ00286.

Expressioni

Gene expression databases

BgeeiQ00286.
GenevestigatoriQ00286.

Interactioni

Protein-protein interaction databases

DIPiDIP-60278N.
STRINGi10090.ENSMUSP00000004964.

Structurei

3D structure databases

ProteinModelPortaliQ00286.
SMRiQ00286. Positions 129-273.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 19875POU-specific
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG241031.
GeneTreeiENSGT00730000110809.
HOGENOMiHOG000116302.
HOVERGENiHBG053120.
KOiK09363.
OrthoDBiEOG7DJSMG.
PhylomeDBiQ00286.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF82. PTHR11636:SF82. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q00286-1) [UniParc]FASTAAdd to Basket

Also known as: Pit-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSCQSFTSAD TFITLNSDAS AALPLRMHHS AAECLPASNH ATNVMSTATG    50
LHYSVPSCHY GNQPSTYGVM AGSLTPCLYK FPDHTLSHGF PPLHQPLLAE 100
DPAASEFKQE LRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKLGYTQ 150
TNVGEALAAV HGSEFSQTTI CRFENLQLSF KNACKLKAIL SKWLEEAEQV 200
GALYNEKVGA NERKRKRRTT ISVAAKDALE RHFGEHSKPS SQEIMRMAEE 250
LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R 291
Length:291
Mass (Da):32,885
Last modified:December 1, 1992 - v1
Checksum:iE2C265F4353E84C5
GO
Isoform 2 (identifier: Q00286-2) [UniParc]FASTAAdd to Basket

Also known as: Pit-1T

The sequence of this isoform differs from the canonical sequence as follows:
     48-48: A → GLHTYFSMTTMGNTA

Show »
Length:305
Mass (Da):34,428
Checksum:i965F1066F30DDE0B
GO
Isoform 3 (identifier: Q00286-3) [UniParc]FASTAAdd to Basket

Also known as: Pit-1a, Pit-1 beta

The sequence of this isoform differs from the canonical sequence as follows:
     48-48: A → VPSILSLIQTPKCLHTYFSMTTMGNTA

Note: Unable to transactivate. Only about 1/7 as abundant as isoform 1.

Show »
Length:317
Mass (Da):35,752
Checksum:i5AE06A891EFBB18B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti261 – 2611W → C in dw. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei48 – 481A → GLHTYFSMTTMGNTA in isoform 2.
VSP_012550
Alternative sequencei48 – 481A → VPSILSLIQTPKCLHTYFSM TTMGNTA in isoform 3.
VSP_012551

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D12885 mRNA. Translation: BAA02289.1.
X57512 mRNA. Translation: CAA40737.1.
BC061213 mRNA. Translation: AAH61213.1.
CCDSiCCDS57033.1. [Q00286-1]
PIRiS11663.
RefSeqiNP_032875.1. NM_008849.4. [Q00286-1]
UniGeneiMm.355440.

Genome annotation databases

EnsembliENSMUST00000176330; ENSMUSP00000135113; ENSMUSG00000004842. [Q00286-1]
GeneIDi18736.
KEGGimmu:18736.
UCSCiuc007zqi.1. mouse. [Q00286-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D12885 mRNA. Translation: BAA02289.1 .
X57512 mRNA. Translation: CAA40737.1 .
BC061213 mRNA. Translation: AAH61213.1 .
CCDSi CCDS57033.1. [Q00286-1 ]
PIRi S11663.
RefSeqi NP_032875.1. NM_008849.4. [Q00286-1 ]
UniGenei Mm.355440.

3D structure databases

ProteinModelPortali Q00286.
SMRi Q00286. Positions 129-273.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60278N.
STRINGi 10090.ENSMUSP00000004964.

PTM databases

PhosphoSitei Q00286.

Proteomic databases

PRIDEi Q00286.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000176330 ; ENSMUSP00000135113 ; ENSMUSG00000004842 . [Q00286-1 ]
GeneIDi 18736.
KEGGi mmu:18736.
UCSCi uc007zqi.1. mouse. [Q00286-1 ]

Organism-specific databases

CTDi 5449.
MGIi MGI:97588. Pou1f1.

Phylogenomic databases

eggNOGi NOG241031.
GeneTreei ENSGT00730000110809.
HOGENOMi HOG000116302.
HOVERGENi HBG053120.
KOi K09363.
OrthoDBi EOG7DJSMG.
PhylomeDBi Q00286.

Miscellaneous databases

NextBioi 294867.
PROi Q00286.
SOURCEi Search...

Gene expression databases

Bgeei Q00286.
Genevestigatori Q00286.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view ]
PANTHERi PTHR11636:SF82. PTHR11636:SF82. 1 hit.
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Dwarf locus mutants lacking three pituitary cell types result from mutations in the POU-domain gene pit-1."
    Li S., Crenshaw E.B. III, Rawson E.J., Simmons D.M., Swanson L.W., Rosenfeld M.G.
    Nature 347:528-533(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT DW CYS-261.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Pituitary.
  3. "An alternatively spliced Pit-1 isoform altered in its ability to trans-activate."
    Morris A.E., Kloss B., McChesney R.E., Bancroft C., Chasin L.A.
    Nucleic Acids Res. 20:1355-1361(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING (ISOFORM 3).
    Tissue: Pituitary.
  4. "A thyrotrope-specific variant of Pit-1 transactivates the thyrotropin beta promoter."
    Haugen B.R., Wood W.M., Gordon D.F., Ridgway E.C.
    J. Biol. Chem. 268:20818-20824(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING (ISOFORM 2).

Entry informationi

Entry nameiPIT1_MOUSE
AccessioniPrimary (citable) accession number: Q00286
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 9, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi