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Q00286 (PIT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pituitary-specific positive transcription factor 1
Short name=PIT-1
Alternative name(s):
Growth hormone factor 1
Short name=GHF-1
Gene names
Name:Pou1f1
Synonyms:Pit-1, Pit1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.

Subcellular location

Nucleus.

Involvement in disease

Defects in Pou1f1 are the cause of the dwarf (dw) phenotype which interrupts the normal development of the anterior pituitary gland, resulting in the loss of expression of growth hormone, prolactin and thyroid-stimulating hormone, and hypoplasia of their respective cell types.

Sequence similarities

Belongs to the POU transcription factor family. Class-1 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DiseaseDisease mutation
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processB cell differentiation

Inferred from mutant phenotype PubMed 9300691. Source: MGI

cell fate specification

Inferred from direct assay PubMed 10367888. Source: MGI

determination of adult lifespan

Inferred from mutant phenotype PubMed 11371619. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Compara

nuclear transport

Inferred from electronic annotation. Source: Compara

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 16556738. Source: MGI

positive regulation of inositol trisphosphate biosynthetic process

Inferred from mutant phenotype PubMed 11371619. Source: MGI

positive regulation of multicellular organism growth

Inferred from mutant phenotype PubMed 17015435PubMed 7390396. Source: MGI

regulation of insulin-like growth factor receptor signaling pathway

Inferred from mutant phenotype PubMed 7390396. Source: MGI

somatotropin secreting cell development

Inferred from mutant phenotype PubMed 17015435. Source: MGI

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Compara

transcription factor complex

Inferred from direct assay PubMed 17392792. Source: MGI

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 9305891. Source: MGI

RNA polymerase II transcription coactivator activity

Inferred from electronic annotation. Source: Compara

chromatin binding

Inferred from direct assay PubMed 16678101PubMed 17015435. Source: MGI

sequence-specific DNA binding

Inferred from direct assay PubMed 7902581. Source: MGI

transcription corepressor activity

Inferred from electronic annotation. Source: Compara

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q00286-1)

Also known as: Pit-1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q00286-2)

Also known as: Pit-1T;

The sequence of this isoform differs from the canonical sequence as follows:
     48-48: A → GLHTYFSMTTMGNTA
Isoform 3 (identifier: Q00286-3)

Also known as: Pit-1a; Pit-1 beta;

The sequence of this isoform differs from the canonical sequence as follows:
     48-48: A → VPSILSLIQTPKCLHTYFSMTTMGNTA
Note: Unable to transactivate. Only about 1/7 as abundant as isoform 1.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Pituitary-specific positive transcription factor 1
PRO_0000100699

Regions

Domain124 – 19875POU-specific
DNA binding214 – 27360Homeobox

Natural variations

Alternative sequence481A → GLHTYFSMTTMGNTA in isoform 2.
VSP_012550
Alternative sequence481A → VPSILSLIQTPKCLHTYFSM TTMGNTA in isoform 3.
VSP_012551
Natural variant2611W → C in dw. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Pit-1) [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: E2C265F4353E84C5

FASTA29132,885
        10         20         30         40         50         60 
MSCQSFTSAD TFITLNSDAS AALPLRMHHS AAECLPASNH ATNVMSTATG LHYSVPSCHY 

        70         80         90        100        110        120 
GNQPSTYGVM AGSLTPCLYK FPDHTLSHGF PPLHQPLLAE DPAASEFKQE LRRKSKLVEE 

       130        140        150        160        170        180 
PIDMDSPEIR ELEQFANEFK VRRIKLGYTQ TNVGEALAAV HGSEFSQTTI CRFENLQLSF 

       190        200        210        220        230        240 
KNACKLKAIL SKWLEEAEQV GALYNEKVGA NERKRKRRTT ISVAAKDALE RHFGEHSKPS 

       250        260        270        280        290 
SQEIMRMAEE LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R

« Hide

Isoform 2 (Pit-1T) [UniParc].

Checksum: 965F1066F30DDE0B
Show »

FASTA30534,428
Isoform 3 (Pit-1a) (Pit-1 beta) [UniParc].

Checksum: 5AE06A891EFBB18B
Show »

FASTA31735,752

References

« Hide 'large scale' references
[1]"Dwarf locus mutants lacking three pituitary cell types result from mutations in the POU-domain gene pit-1."
Li S., Crenshaw E.B. III, Rawson E.J., Simmons D.M., Swanson L.W., Rosenfeld M.G.
Nature 347:528-533(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT DW CYS-261.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Pituitary.
[3]"An alternatively spliced Pit-1 isoform altered in its ability to trans-activate."
Morris A.E., Kloss B., McChesney R.E., Bancroft C., Chasin L.A.
Nucleic Acids Res. 20:1355-1361(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORM 3).
Tissue: Pituitary.
[4]"A thyrotrope-specific variant of Pit-1 transactivates the thyrotropin beta promoter."
Haugen B.R., Wood W.M., Gordon D.F., Ridgway E.C.
J. Biol. Chem. 268:20818-20824(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORM 2).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D12885 mRNA. Translation: BAA02289.1.
X57512 mRNA. Translation: CAA40737.1.
BC061213 mRNA. Translation: AAH61213.1.
IPIIPI00117165.
IPI00515322.
IPI00515372.
PIRS11663.
RefSeqNP_032875.1. NM_008849.4.
UniGeneMm.355440.

3D structure databases

ProteinModelPortalQ00286.
SMRQ00286. Positions 129-273.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000004964.

PTM databases

PhosphoSiteQ00286.

Proteomic databases

PRIDEQ00286.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000176330; ENSMUSP00000135113; ENSMUSG00000004842.
GeneID18736.
KEGGmmu:18736.
UCSCuc007zqi.1. mouse.

Organism-specific databases

CTD5449.
MGIMGI:97588. Pou1f1.

Phylogenomic databases

eggNOGNOG241031.
GeneTreeENSGT00700000104015.
HOGENOMHOG000116302.
HOVERGENHBG053120.
KOK09363.
OrthoDBEOG4JQ3Z4.

Gene expression databases

ArrayExpressQ00286.
BgeeQ00286.
GenevestigatorQ00286.
GermOnlineENSMUSG00000004842. Mus musculus.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeodomain.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PANTHERPTHR11636:SF17. PTHR11636:SF17. 1 hit.
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. Homeodomain_like. 1 hit.
SSF47413. Lambda_like_DNA. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio294867.
SOURCESearch...

Entry information

Entry namePIT1_MOUSE
AccessionPrimary (citable) accession number: Q00286
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 3, 2013
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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