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Protein

Pituitary-specific positive transcription factor 1

Gene

Pou1f1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi214 – 27360HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • B cell differentiation Source: MGI
  • cell fate specification Source: MGI
  • determination of adult lifespan Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • nuclear transport Source: Ensembl
  • pituitary gland development Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of inositol trisphosphate biosynthetic process Source: MGI
  • positive regulation of multicellular organism growth Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of insulin-like growth factor receptor signaling pathway Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • somatotropin secreting cell development Source: MGI
  • somatotropin secreting cell differentiation Source: MGI
  • transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary-specific positive transcription factor 1
Short name:
PIT-1
Alternative name(s):
Growth hormone factor 1
Short name:
GHF-1
Gene namesi
Name:Pou1f1
Synonyms:Pit-1, Pit1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:97588. Pou1f1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Pou1f1 are the cause of the dwarf (dw) phenotype which interrupts the normal development of the anterior pituitary gland, resulting in the loss of expression of growth hormone, prolactin and thyroid-stimulating hormone, and hypoplasia of their respective cell types.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Pituitary-specific positive transcription factor 1PRO_0000100699Add
BLAST

Proteomic databases

PRIDEiQ00286.

PTM databases

PhosphoSiteiQ00286.

Expressioni

Gene expression databases

BgeeiQ00286.
ExpressionAtlasiQ00286. baseline.
GenevisibleiQ00286. MM.

Interactioni

Protein-protein interaction databases

DIPiDIP-60278N.
STRINGi10090.ENSMUSP00000135113.

Structurei

3D structure databases

ProteinModelPortaliQ00286.
SMRiQ00286. Positions 129-273.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 19875POU-specificPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG241031.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116302.
HOVERGENiHBG053120.
InParanoidiQ00286.
KOiK09363.
OrthoDBiEOG7DJSMG.
PhylomeDBiQ00286.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF82. PTHR11636:SF82. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q00286-1) [UniParc]FASTAAdd to basket

Also known as: Pit-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSCQSFTSAD TFITLNSDAS AALPLRMHHS AAECLPASNH ATNVMSTATG
60 70 80 90 100
LHYSVPSCHY GNQPSTYGVM AGSLTPCLYK FPDHTLSHGF PPLHQPLLAE
110 120 130 140 150
DPAASEFKQE LRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKLGYTQ
160 170 180 190 200
TNVGEALAAV HGSEFSQTTI CRFENLQLSF KNACKLKAIL SKWLEEAEQV
210 220 230 240 250
GALYNEKVGA NERKRKRRTT ISVAAKDALE RHFGEHSKPS SQEIMRMAEE
260 270 280 290
LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R
Length:291
Mass (Da):32,885
Last modified:December 1, 1992 - v1
Checksum:iE2C265F4353E84C5
GO
Isoform 2 (identifier: Q00286-2) [UniParc]FASTAAdd to basket

Also known as: Pit-1T

The sequence of this isoform differs from the canonical sequence as follows:
     48-48: A → GLHTYFSMTTMGNTA

Show »
Length:305
Mass (Da):34,428
Checksum:i965F1066F30DDE0B
GO
Isoform 3 (identifier: Q00286-3) [UniParc]FASTAAdd to basket

Also known as: Pit-1a, Pit-1 beta

The sequence of this isoform differs from the canonical sequence as follows:
     48-48: A → VPSILSLIQTPKCLHTYFSMTTMGNTA

Note: Unable to transactivate. Only about 1/7 as abundant as isoform 1.
Show »
Length:317
Mass (Da):35,752
Checksum:i5AE06A891EFBB18B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti261 – 2611W → C in dw. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei48 – 481A → GLHTYFSMTTMGNTA in isoform 2. CuratedVSP_012550
Alternative sequencei48 – 481A → VPSILSLIQTPKCLHTYFSM TTMGNTA in isoform 3. CuratedVSP_012551

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12885 mRNA. Translation: BAA02289.1.
X57512 mRNA. Translation: CAA40737.1.
BC061213 mRNA. Translation: AAH61213.1.
CCDSiCCDS57033.1. [Q00286-1]
PIRiS11663.
RefSeqiNP_032875.1. NM_008849.4. [Q00286-1]
UniGeneiMm.355440.

Genome annotation databases

EnsembliENSMUST00000176330; ENSMUSP00000135113; ENSMUSG00000004842. [Q00286-1]
GeneIDi18736.
KEGGimmu:18736.
UCSCiuc007zqi.1. mouse. [Q00286-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12885 mRNA. Translation: BAA02289.1.
X57512 mRNA. Translation: CAA40737.1.
BC061213 mRNA. Translation: AAH61213.1.
CCDSiCCDS57033.1. [Q00286-1]
PIRiS11663.
RefSeqiNP_032875.1. NM_008849.4. [Q00286-1]
UniGeneiMm.355440.

3D structure databases

ProteinModelPortaliQ00286.
SMRiQ00286. Positions 129-273.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60278N.
STRINGi10090.ENSMUSP00000135113.

PTM databases

PhosphoSiteiQ00286.

Proteomic databases

PRIDEiQ00286.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000176330; ENSMUSP00000135113; ENSMUSG00000004842. [Q00286-1]
GeneIDi18736.
KEGGimmu:18736.
UCSCiuc007zqi.1. mouse. [Q00286-1]

Organism-specific databases

CTDi5449.
MGIiMGI:97588. Pou1f1.

Phylogenomic databases

eggNOGiNOG241031.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116302.
HOVERGENiHBG053120.
InParanoidiQ00286.
KOiK09363.
OrthoDBiEOG7DJSMG.
PhylomeDBiQ00286.

Miscellaneous databases

NextBioi294867.
PROiQ00286.
SOURCEiSearch...

Gene expression databases

BgeeiQ00286.
ExpressionAtlasiQ00286. baseline.
GenevisibleiQ00286. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF82. PTHR11636:SF82. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Dwarf locus mutants lacking three pituitary cell types result from mutations in the POU-domain gene pit-1."
    Li S., Crenshaw E.B. III, Rawson E.J., Simmons D.M., Swanson L.W., Rosenfeld M.G.
    Nature 347:528-533(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT DW CYS-261.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Pituitary.
  3. "An alternatively spliced Pit-1 isoform altered in its ability to trans-activate."
    Morris A.E., Kloss B., McChesney R.E., Bancroft C., Chasin L.A.
    Nucleic Acids Res. 20:1355-1361(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING (ISOFORM 3).
    Tissue: Pituitary.
  4. "A thyrotrope-specific variant of Pit-1 transactivates the thyrotropin beta promoter."
    Haugen B.R., Wood W.M., Gordon D.F., Ridgway E.C.
    J. Biol. Chem. 268:20818-20824(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING (ISOFORM 2).

Entry informationi

Entry nameiPIT1_MOUSE
AccessioniPrimary (citable) accession number: Q00286
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 22, 2015
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.