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Protein

N-hydroxyarylamine O-acetyltransferase

Gene

nhoA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes both the acetyl-CoA-dependent N-acetylation of aromatic amines and the O-acetylation of N-hydroxyarylamines. In vitro, catalyzes the O-acetylation of N-hydroxy-Glu-P-1, and the N-acetylation of isoniazid and 2-aminofluorene.2 Publications

Catalytic activityi

Acetyl-CoA + an N-hydroxyarylamine = CoA + an N-acetoxyarylamine.2 Publications

Enzyme regulationi

Inhibited by N-ethylmaleimide and iodoacetamide.2 Publications

Kineticsi

  1. KM=10 µM for acetyl-CoA (in the presence of N-hydroxy-Glu-P-1)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei69Acyl-thioester intermediate3 Publications1
    Active sitei1071 Publication1
    Active sitei1221 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-hydroxyarylamine O-acetyltransferase1 Publication (EC:2.3.1.1182 Publications)
    Alternative name(s):
    Arylamine N-acetyltransferase1 Publication
    Arylhydroxamate N,O-acetyltransferaseCurated
    NAT101
    Gene namesi
    Name:nhoA
    Ordered Locus Names:STM1582
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
    Proteomesi
    • UP000001014 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm 2 Publications

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi69C → A: Loss of O- and N-acetyltransferase activities. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001079161 – 281N-hydroxyarylamine O-acetyltransferaseAdd BLAST281

    Proteomic databases

    PaxDbiQ00267.
    PRIDEiQ00267.

    Interactioni

    Subunit structurei

    Monomer and homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi99287.STM1582.

    Structurei

    Secondary structure

    1281
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi3 – 11Combined sources9
    Helixi22 – 35Combined sources14
    Helixi41 – 44Combined sources4
    Helixi55 – 60Combined sources6
    Turni61 – 63Combined sources3
    Helixi69 – 82Combined sources14
    Beta strandi87 – 94Combined sources8
    Beta strandi107 – 114Combined sources8
    Beta strandi117 – 121Combined sources5
    Beta strandi134 – 136Combined sources3
    Beta strandi142 – 144Combined sources3
    Beta strandi147 – 152Combined sources6
    Beta strandi157 – 174Combined sources18
    Helixi181 – 193Combined sources13
    Helixi198 – 200Combined sources3
    Beta strandi204 – 208Combined sources5
    Beta strandi210 – 212Combined sources3
    Beta strandi214 – 218Combined sources5
    Beta strandi221 – 226Combined sources6
    Helixi239 – 248Combined sources10
    Turni257 – 259Combined sources3
    Helixi263 – 271Combined sources9

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1E2TX-ray2.80A/B/C/D/E/F/G/H1-281[»]
    ProteinModelPortaliQ00267.
    SMRiQ00267.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ00267.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4108MUX. Bacteria.
    COG2162. LUCA.
    HOGENOMiHOG000205436.
    KOiK00675.
    OMAiHAGLYES.
    PhylomeDBiQ00267.

    Family and domain databases

    InterProiIPR001447. Arylamine_N-AcTrfase.
    [Graphical view]
    PANTHERiPTHR11786. PTHR11786. 1 hit.
    PfamiPF00797. Acetyltransf_2. 1 hit.
    [Graphical view]
    PRINTSiPR01543. ANATRNSFRASE.

    Sequencei

    Sequence statusi: Complete.

    Q00267-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTSFLHAYFT RLHCQPLGVP TVEALRTLHL AHNCAIPFEN LDVLLPREIQ
    60 70 80 90 100
    LDETALEEKL LYARRGGYCF ELNGLFERAL RDIGFNVRSL LGRVILSHPA
    110 120 130 140 150
    SLPPRTHRLL LVDVEDEQWI ADVGFGGQTL TAPLRLQAEI AQQTPHGEYR
    160 170 180 190 200
    LMQEGSTWIL QFRHHEHWQS MYCFDLGVQQ QSDHVMGNFW SAHWPQSHFR
    210 220 230 240 250
    HHLLMCRHLP DGGKLTLTNF HFTRYHQGHA VEQVNVPDVP SLYQLLQQQF
    260 270 280
    GLGVNDVKHG FTEAELAAVM AAFDTHPEAG K
    Length:281
    Mass (Da):32,178
    Last modified:December 1, 1992 - v1
    Checksum:i5FB3B667BD4D7804
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D90301 Genomic DNA. Translation: BAA14331.1.
    S76130 Genomic DNA. Translation: AAB33787.1.
    AE006468 Genomic DNA. Translation: AAL20500.1.
    PIRiA38090.
    RefSeqiNP_460541.1. NC_003197.1.
    WP_000200329.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL20500; AAL20500; STM1582.
    GeneIDi1253100.
    KEGGistm:STM1582.
    PATRICi32381666. VBISalEnt20916_1673.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D90301 Genomic DNA. Translation: BAA14331.1.
    S76130 Genomic DNA. Translation: AAB33787.1.
    AE006468 Genomic DNA. Translation: AAL20500.1.
    PIRiA38090.
    RefSeqiNP_460541.1. NC_003197.1.
    WP_000200329.1. NC_003197.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1E2TX-ray2.80A/B/C/D/E/F/G/H1-281[»]
    ProteinModelPortaliQ00267.
    SMRiQ00267.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi99287.STM1582.

    Proteomic databases

    PaxDbiQ00267.
    PRIDEiQ00267.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAL20500; AAL20500; STM1582.
    GeneIDi1253100.
    KEGGistm:STM1582.
    PATRICi32381666. VBISalEnt20916_1673.

    Phylogenomic databases

    eggNOGiENOG4108MUX. Bacteria.
    COG2162. LUCA.
    HOGENOMiHOG000205436.
    KOiK00675.
    OMAiHAGLYES.
    PhylomeDBiQ00267.

    Miscellaneous databases

    EvolutionaryTraceiQ00267.

    Family and domain databases

    InterProiIPR001447. Arylamine_N-AcTrfase.
    [Graphical view]
    PANTHERiPTHR11786. PTHR11786. 1 hit.
    PfamiPF00797. Acetyltransf_2. 1 hit.
    [Graphical view]
    PRINTSiPR01543. ANATRNSFRASE.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNHOA_SALTY
    AccessioniPrimary (citable) accession number: Q00267
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: November 2, 2016
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.