Reviewed, UniProtKB/Swiss-Prot Q00257 (1A12_CUCMA)

Names and origin

Protein names

Recommended name:
    1-aminocyclopropane-1-carboxylate synthase CMA101
      Short name=ACC synthase
    EC=4.4.1.14
Alternative name(s):
    S-adenosyl-L-methionine methylthioadenosine-lyase

Gene names

Name:	ACS2
Synonyms:	PCVV4A

Organism

Cucurbita maxima (Pumpkin) (Winter squash)

Taxonomic identifier

3661 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Cucurbitales › Cucurbitaceae › Cucurbita

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of 1-aminocyclopropane-1-carboxylate, a direct precursor of ethylene in higher plants.
Catalytic activity	S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + methylthioadenosine.
Cofactor	Pyridoxal phosphate.
Pathway	Alkene biosynthesis; ethylene biosynthesis via S-adenosyl-L-methionine; ethylene from S-adenosyl-L-methionine: step 1/2.
Subunit structure	Homodimer.
Induction	By tissue wounding and auxin.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Ethylene biosynthesis Fruit ripening
Ligand	Pyridoxal phosphate S-adenosyl-L-methionine
Molecular function	Lyase
Gene Ontology (GO)
Biological process	ethylene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW ripening Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-aminocyclopropane-1-carboxylate synthase activity Inferred from sequence or structural similarity. Source: UniProtKB pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	1-aminocyclopropane-1-carboxylate synthase CMA101		PRO_0000123908
Amino acid modifications
	Modified residue	272	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q00257-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: D02A666E137F44A0	FASTA	475	53,481
	10 20 30 40 50 60 MKMLSTKATC NSHGQDSSYF LGWEAYENNP FHHTSNPNGI IQMGLAENQL SFDLLESWLS 70 80 90 100 110 120 KNPDAASFKR DGKSIFRELA LFQDYHGLPA FKKALVEFMA EIRGNKVSFE ANNIVLTAGA 130 140 150 160 170 180 TSANETLMFC LAEAGDAFLL PTPYYPGFDR DLKWRTGVEI VPIHCTSSNG FQITQSALEQ 190 200 210 220 230 240 AYKDAQTRNL RVKGVLVTNP SNPLGTTMNR DELNLVFDFI TSKGIHLISD EIYSGTVFGS 250 260 270 280 290 300 PGFVSAMEVL KERSSEDEEV WKRVHIVYSL SKDLGLPGFR VGAIYSNDDM VVAAATKMSS 310 320 330 340 350 360 FGLVSSQTQY LLSAMLSDKK FTISYISENQ KRLKQRQKML VSGLQKAGIN CLDSNAGLFC 370 380 390 400 410 420 WVDMRHLLES DKFESELELW KKIVYEVGLN ISPGSSCHCT EPGWFRVCFA NMSESTLKLA 430 440 450 460 470 VRRLKSFVTE LRSTTTSNHR NHDNKICKNI KKNIFTKWVF RQSAQEANRK MQAER

References

[1]	"Cloning of a complementary DNA for auxin-induced 1-aminocyclopropane-1-carboxylate synthase and differential expression of the gene by auxin and wounding." Nakagawa N., Mori H., Yamazaki K., Imaseki H. Plant Cell Physiol. 32:1153-1163(1991) Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Nucleotide sequence of an auxin-regulated 1-aminocyclopropane-1-carboxylic acid synthase gene from Cucurbita maxima Duch." Nakagawa N., Kamiya Y., Imaseki H. Plant Gene Register PGR95-110 Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U37774 Genomic DNA. Translation: AAA91152.1. D01033 mRNA. Translation: BAA00839.1.
PIR	JQ2214.
3D structure databases
SMR	Q00257. Positions 12-430.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.4.1.14. 2176.
Family and domain databases
InterPro	IPR001176. ACC_synthase. IPR004839. Aminotransferase_I/II. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00753. ACCSYNTHASE.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

1A12_CUCMA

Accession

Primary (citable) accession number: Q00257

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)