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Q00246 (RHO4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 142. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP-binding protein RHO4
Gene names
Name:RHO4
Ordered Locus Names:YKR055W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in cell growth. Required to keep the uninucleated state. May be involved in the organization of the cytoskeleton which affects microtubule functions. Most likely RHO3 and RHO4 of S.cerevisiae regulate partially overlapping but different pathways. Ref.7

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

Sequence similarities

Belongs to the small GTPase superfamily. Rho family.

Sequence caution

The sequence CAA78500.1 differs from that shown. Reason: Frameshift at position 263. Leads to a fusion with RNC1.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 288288GTP-binding protein RHO4
PRO_0000198948
Propeptide289 – 2913Removed in mature form By similarity
PRO_0000281278

Regions

Nucleotide binding79 – 868GTP By similarity
Nucleotide binding127 – 1315GTP By similarity
Nucleotide binding185 – 1884GTP By similarity
Motif101 – 1099Effector region By similarity

Amino acid modifications

Modified residue2641Phosphoserine Ref.9
Modified residue2681Phosphoserine Ref.9
Modified residue2761Phosphoserine Ref.8 Ref.9
Modified residue2881Cysteine methyl ester By similarity
Lipidation2881S-farnesyl cysteine By similarity

Experimental info

Sequence conflict43 – 486PRLPTP → QIAYS in CAA78500. Ref.3
Sequence conflict1431T → R in CAA78500. Ref.3
Sequence conflict201 – 21111PSSAESLAKRL → QVQQNPWPSVW AA sequence Ref.3
Sequence conflict213 – 2186AFAHIQ → HLHIFK AA sequence Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q00246 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: 4B7BA75EA9BA50FD

FASTA29132,186
        10         20         30         40         50         60 
MNTLLFKRKG GNCGNESNIV SQGSPSSSNL PESPGTLDEK NLPRLPTPFA RSLSTIPSYE 

        70         80         90        100        110        120 
QMKRTNKLPD YHLKIVVVGD GAVGKTCLLI SYVQGTFPTD YIPTIFENYV TNIEGPNGQI 

       130        140        150        160        170        180 
IELALWDTAG QEEYSRLRPL SYTNADVLMV CYSVGSKTSL KNVEDLWFPE VKHFCPSTPI 

       190        200        210        220        230        240 
MLVGLKSDLY EADNLSDLVE PSSAESLAKR LGAFAHIQCS ARLKENIDEV FETAIHTLLS 

       250        260        270        280        290 
DSLYAPREPT HTIKNPFKRN TTRSDIDSST GDTSVSISGT KRLRKNKCII M 

« Hide

References

« Hide 'large scale' references
[1]"The RHO4a and NUD1 genes on Saccharomyces cerevisiae chromosome XI."
van Vliet-Reedijk J.C., Planta R.J.
Yeast 9:1139-1147(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Isolation and characterization of two novel ras superfamily genes in Saccharomyces cerevisiae."
Matsui Y., Toh-E A.
Gene 114:43-49(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 62-291.
Strain: ATCC 38626 / AH22 / NRRL Y-12843.
[3]"Yeast RNC1 encodes a chimeric protein, RhoNUC, with a human rho motif and deoxyribonuclease activity."
Chow T.Y.-K., Perkins E.L., Resnick M.A.
Nucleic Acids Res. 20:5215-5221(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 38-42.
[4]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[7]"Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1."
Matsui Y., Toh-E A.
Mol. Cell. Biol. 12:5690-5699(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264; SER-268 AND SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z25734 Genomic DNA. Translation: CAA81020.1.
D10007 Genomic DNA. Translation: BAA00898.1.
Z14126 Genomic DNA. Translation: CAA78500.1. Frameshift.
Z28280 Genomic DNA. Translation: CAA82133.1.
AY557909 Genomic DNA. Translation: AAS56235.1.
BK006944 Genomic DNA. Translation: DAA09206.1.
PIRS37743.
RefSeqNP_012981.3. NM_001179845.3.

3D structure databases

ProteinModelPortalQ00246.
SMRQ00246. Positions 74-234.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34186. 68 interactions.
DIPDIP-755N.
IntActQ00246. 3 interactions.
MINTMINT-390167.

Proteomic databases

MaxQBQ00246.
PaxDbQ00246.
PeptideAtlasQ00246.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR055W; YKR055W; YKR055W.
GeneID853929.
KEGGsce:YKR055W.

Organism-specific databases

CYGDYKR055w.
SGDS000001763. RHO4.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00750000118076.
HOGENOMHOG000233974.
KOK07975.
OMAVTNIEGP.
OrthoDBEOG7SN8QP.

Enzyme and pathway databases

BioCycYEAST:G3O-32024-MONOMER.

Gene expression databases

GenevestigatorQ00246.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975294.

Entry information

Entry nameRHO4_YEAST
AccessionPrimary (citable) accession number: Q00246
Secondary accession number(s): D6VXB6, P30618
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families