Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GTP-binding protein RHO4

Gene

RHO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in cell growth. Required to keep the uninucleated state. May be involved in the organization of the cytoskeleton which affects microtubule functions. Most likely RHO3 and RHO4 of S.cerevisiae regulate partially overlapping but different pathways.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi79 – 86GTPBy similarity8
Nucleotide bindingi127 – 131GTPBy similarity5
Nucleotide bindingi185 – 188GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • maintenance of cell polarity Source: SGD
  • positive regulation of formin-nucleated actin cable assembly Source: SGD
  • regulation of formin-nucleated actin cable assembly Source: SGD
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32024-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein RHO4
Gene namesi
Name:RHO4
Ordered Locus Names:YKR055W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR055W.
SGDiS000001763. RHO4.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • incipient cellular bud site Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001989481 – 288GTP-binding protein RHO4Add BLAST288
PropeptideiPRO_0000281278289 – 291Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei264PhosphoserineCombined sources1
Modified residuei268PhosphoserineCombined sources1
Modified residuei276PhosphoserineCombined sources1
Modified residuei288Cysteine methyl esterBy similarity1
Lipidationi288S-farnesyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ00246.
PRIDEiQ00246.

PTM databases

iPTMnetiQ00246.

Interactioni

Protein-protein interaction databases

BioGridi34186. 68 interactors.
DIPiDIP-755N.
IntActiQ00246. 3 interactors.
MINTiMINT-390167.

Structurei

3D structure databases

ProteinModelPortaliQ00246.
SMRiQ00246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi101 – 109Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

GeneTreeiENSGT00830000128644.
HOGENOMiHOG000233974.
InParanoidiQ00246.
KOiK07975.
OMAiVTNIEGP.
OrthoDBiEOG092C4TDG.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00246-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTLLFKRKG GNCGNESNIV SQGSPSSSNL PESPGTLDEK NLPRLPTPFA
60 70 80 90 100
RSLSTIPSYE QMKRTNKLPD YHLKIVVVGD GAVGKTCLLI SYVQGTFPTD
110 120 130 140 150
YIPTIFENYV TNIEGPNGQI IELALWDTAG QEEYSRLRPL SYTNADVLMV
160 170 180 190 200
CYSVGSKTSL KNVEDLWFPE VKHFCPSTPI MLVGLKSDLY EADNLSDLVE
210 220 230 240 250
PSSAESLAKR LGAFAHIQCS ARLKENIDEV FETAIHTLLS DSLYAPREPT
260 270 280 290
HTIKNPFKRN TTRSDIDSST GDTSVSISGT KRLRKNKCII M
Length:291
Mass (Da):32,186
Last modified:February 1, 1994 - v2
Checksum:i4B7BA75EA9BA50FD
GO

Sequence cautioni

The sequence CAA78500 differs from that shown. Reason: Frameshift at position 263. Leads to a fusion with RNC1.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43 – 48PRLPTP → QIAYS in CAA78500 (PubMed:1408836).Curated6
Sequence conflicti143T → R in CAA78500 (PubMed:1408836).Curated1
Sequence conflicti201 – 211PSSAESLAKRL → QVQQNPWPSVW AA sequence (PubMed:1408836).CuratedAdd BLAST11
Sequence conflicti213 – 218AFAHIQ → HLHIFK AA sequence (PubMed:1408836).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25734 Genomic DNA. Translation: CAA81020.1.
D10007 Genomic DNA. Translation: BAA00898.1.
Z14126 Genomic DNA. Translation: CAA78500.1. Frameshift.
Z28280 Genomic DNA. Translation: CAA82133.1.
AY557909 Genomic DNA. Translation: AAS56235.1.
BK006944 Genomic DNA. Translation: DAA09206.1.
PIRiS37743.
RefSeqiNP_012981.3. NM_001179845.3.

Genome annotation databases

EnsemblFungiiYKR055W; YKR055W; YKR055W.
GeneIDi853929.
KEGGisce:YKR055W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25734 Genomic DNA. Translation: CAA81020.1.
D10007 Genomic DNA. Translation: BAA00898.1.
Z14126 Genomic DNA. Translation: CAA78500.1. Frameshift.
Z28280 Genomic DNA. Translation: CAA82133.1.
AY557909 Genomic DNA. Translation: AAS56235.1.
BK006944 Genomic DNA. Translation: DAA09206.1.
PIRiS37743.
RefSeqiNP_012981.3. NM_001179845.3.

3D structure databases

ProteinModelPortaliQ00246.
SMRiQ00246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34186. 68 interactors.
DIPiDIP-755N.
IntActiQ00246. 3 interactors.
MINTiMINT-390167.

PTM databases

iPTMnetiQ00246.

Proteomic databases

MaxQBiQ00246.
PRIDEiQ00246.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR055W; YKR055W; YKR055W.
GeneIDi853929.
KEGGisce:YKR055W.

Organism-specific databases

EuPathDBiFungiDB:YKR055W.
SGDiS000001763. RHO4.

Phylogenomic databases

GeneTreeiENSGT00830000128644.
HOGENOMiHOG000233974.
InParanoidiQ00246.
KOiK07975.
OMAiVTNIEGP.
OrthoDBiEOG092C4TDG.

Enzyme and pathway databases

BioCyciYEAST:G3O-32024-MONOMER.

Miscellaneous databases

PROiQ00246.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHO4_YEAST
AccessioniPrimary (citable) accession number: Q00246
Secondary accession number(s): D6VXB6, P30618
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 165 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.