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Protein

GTP-binding protein RHO4

Gene

RHO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in cell growth. Required to keep the uninucleated state. May be involved in the organization of the cytoskeleton which affects microtubule functions. Most likely RHO3 and RHO4 of S.cerevisiae regulate partially overlapping but different pathways.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi79 – 86GTPBy similarity8
Nucleotide bindingi127 – 131GTPBy similarity5
Nucleotide bindingi185 – 188GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • maintenance of cell polarity Source: SGD
  • positive regulation of formin-nucleated actin cable assembly Source: SGD
  • regulation of formin-nucleated actin cable assembly Source: SGD
  • small GTPase mediated signal transduction Source: InterPro

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32024-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein RHO4
Gene namesi
Name:RHO4
Ordered Locus Names:YKR055W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR055W
SGDiS000001763 RHO4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001989481 – 288GTP-binding protein RHO4Add BLAST288
PropeptideiPRO_0000281278289 – 291Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei264PhosphoserineCombined sources1
Modified residuei268PhosphoserineCombined sources1
Modified residuei276PhosphoserineCombined sources1
Modified residuei288Cysteine methyl esterBy similarity1
Lipidationi288S-farnesyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ00246
PaxDbiQ00246
PRIDEiQ00246

PTM databases

iPTMnetiQ00246

Interactioni

Protein-protein interaction databases

BioGridi34186, 129 interactors
DIPiDIP-755N
IntActiQ00246, 16 interactors
MINTiQ00246
STRINGi4932.YKR055W

Structurei

3D structure databases

ProteinModelPortaliQ00246
SMRiQ00246
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi101 – 109Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

GeneTreeiENSGT00910000144594
HOGENOMiHOG000233974
InParanoidiQ00246
KOiK07975
OMAiVTNIEGP
OrthoDBiEOG092C4TDG

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00246-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTLLFKRKG GNCGNESNIV SQGSPSSSNL PESPGTLDEK NLPRLPTPFA
60 70 80 90 100
RSLSTIPSYE QMKRTNKLPD YHLKIVVVGD GAVGKTCLLI SYVQGTFPTD
110 120 130 140 150
YIPTIFENYV TNIEGPNGQI IELALWDTAG QEEYSRLRPL SYTNADVLMV
160 170 180 190 200
CYSVGSKTSL KNVEDLWFPE VKHFCPSTPI MLVGLKSDLY EADNLSDLVE
210 220 230 240 250
PSSAESLAKR LGAFAHIQCS ARLKENIDEV FETAIHTLLS DSLYAPREPT
260 270 280 290
HTIKNPFKRN TTRSDIDSST GDTSVSISGT KRLRKNKCII M
Length:291
Mass (Da):32,186
Last modified:February 1, 1994 - v2
Checksum:i4B7BA75EA9BA50FD
GO

Sequence cautioni

The sequence CAA78500 differs from that shown. Reason: Frameshift at position 263. Leads to a fusion with RNC1.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43 – 48PRLPTP → QIAYS in CAA78500 (PubMed:1408836).Curated6
Sequence conflicti143T → R in CAA78500 (PubMed:1408836).Curated1
Sequence conflicti201 – 211PSSAESLAKRL → QVQQNPWPSVW AA sequence (PubMed:1408836).CuratedAdd BLAST11
Sequence conflicti213 – 218AFAHIQ → HLHIFK AA sequence (PubMed:1408836).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25734 Genomic DNA Translation: CAA81020.1
D10007 Genomic DNA Translation: BAA00898.1
Z14126 Genomic DNA Translation: CAA78500.1 Frameshift.
Z28280 Genomic DNA Translation: CAA82133.1
AY557909 Genomic DNA Translation: AAS56235.1
BK006944 Genomic DNA Translation: DAA09206.1
PIRiS37743
RefSeqiNP_012981.3, NM_001179845.3

Genome annotation databases

EnsemblFungiiYKR055W; YKR055W; YKR055W
GeneIDi853929
KEGGisce:YKR055W

Similar proteinsi

Entry informationi

Entry nameiRHO4_YEAST
AccessioniPrimary (citable) accession number: Q00246
Secondary accession number(s): D6VXB6, P30618
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names
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