GTP-binding protein RHO4 precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q00246 (RHO4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 120. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GTP-binding protein RHO4

Gene names

Name:	RHO4
Ordered Locus Names:	YKR055W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in cell growth. Required to keep the uninucleated state. May be involved in the organization of the cytoskeleton which affects microtubule functions. Most likely RHO3 and RHO4 of S.cerevisiae regulate partially overlapping but different pathways. Ref.7
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family.
Sequence caution	The sequence CAA78500.1 differs from that shown. Reason: Frameshift at position 263. Leads to a fusion with RNC1.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	maintenance of cell polarity Inferred from genetic interaction Ref.7. Source: SGD regulation of formin-nucleated actin cable assembly Inferred from physical interaction. Source: SGD small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cellular bud neck Inferred from direct assay. Source: SGD intracellular Inferred from electronic annotation. Source: InterPro membrane fraction Inferred from direct assay. Source: SGD plasma membrane Inferred from direct assay. Source: SGD
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 288

288

GTP-binding protein RHO4

PRO_0000198948

Propeptide

289 – 291

Removed in mature form By similarity

PRO_0000281278

Regions

Nucleotide binding

79 – 86

GTP By similarity

Nucleotide binding

127 – 131

GTP By similarity

Nucleotide binding

185 – 188

GTP By similarity

Motif

101 – 109

Effector region By similarity

Amino acid modifications

Modified residue

Phosphothreonine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphotyrosine Ref.8

Modified residue

276

Phosphoserine Ref.8

Modified residue

278

Phosphoserine Ref.8

Modified residue

288

Cysteine methyl ester By similarity

Lipidation

288

S-farnesyl cysteine By similarity

Experimental info

Sequence conflict

43 – 48

PRLPTP → QIAYS in CAA78500. Ref.3

Sequence conflict

143

T → R in CAA78500. Ref.3

Sequence conflict

201 – 211

PSSAESLAKRL → QVQQNPWPSVW AA sequence Ref.3

Sequence conflict

213 – 218

AFAHIQ → HLHIFK AA sequence Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q00246 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: 4B7BA75EA9BA50FD
Show »

FASTA

291

32,186

        10         20         30         40         50         60 
MNTLLFKRKG GNCGNESNIV SQGSPSSSNL PESPGTLDEK NLPRLPTPFA RSLSTIPSYE 

        70         80         90        100        110        120 
QMKRTNKLPD YHLKIVVVGD GAVGKTCLLI SYVQGTFPTD YIPTIFENYV TNIEGPNGQI 

       130        140        150        160        170        180 
IELALWDTAG QEEYSRLRPL SYTNADVLMV CYSVGSKTSL KNVEDLWFPE VKHFCPSTPI 

       190        200        210        220        230        240 
MLVGLKSDLY EADNLSDLVE PSSAESLAKR LGAFAHIQCS ARLKENIDEV FETAIHTLLS 

       250        260        270        280        290 
DSLYAPREPT HTIKNPFKRN TTRSDIDSST GDTSVSISGT KRLRKNKCII M

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The RHO4a and NUD1 genes on Saccharomyces cerevisiae chromosome XI." van Vliet-Reedijk J.C., Planta R.J. Yeast 9:1139-1147(1993) [PubMed: 8256523] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Isolation and characterization of two novel ras superfamily genes in Saccharomyces cerevisiae." Matsui Y., Toh-E A. Gene 114:43-49(1992) [PubMed: 1587484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 62-291. Strain: ATCC 38626 / AH22 / NRRL Y-12843.
[3]	"Yeast RNC1 encodes a chimeric protein, RhoNUC, with a human rho motif and deoxyribonuclease activity." Chow T.Y.-K., Perkins E.L., Resnick M.A. Nucleic Acids Res. 20:5215-5221(1992) [PubMed: 1408836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 38-42.
[4]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1." Matsui Y., Toh-E A. Mol. Cell. Biol. 12:5690-5699(1992) [PubMed: 1448099] [Abstract] Cited for: FUNCTION.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-47; SER-58; TYR-59; SER-276 AND SER-278, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z25734 Genomic DNA. Translation: CAA81020.1. D10007 Genomic DNA. Translation: BAA00898.1. Z14126 Genomic DNA. Translation: CAA78500.1. Frameshift. Z28280 Genomic DNA. Translation: CAA82133.1. AY557909 Genomic DNA. Translation: AAS56235.1. BK006944 Genomic DNA. Translation: DAA09206.1.
PIR	S37743.
RefSeq	NP_012981.1. NM_001179845.1.
3D structure databases
ProteinModelPortal	Q00246.
SMR	Q00246. Positions 70-239.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-755N.
IntAct	Q00246. 4 interactions.
MINT	MINT-390167.
STRING	Q00246.
Proteomic databases
PeptideAtlas	Q00246.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR055W; YKR055W; YKR055W.
GeneID	853929.
KEGG	sce:YKR055W.
NMPDR	fig\|4932.3.peg.3967.
Organism-specific databases
CYGD	YKR055w.
SGD	S000001763. RHO4.
Phylogenomic databases
GeneTree	EFGT00070000008719.
HOGENOM	HBG745225.
OMA	VTNIEGP.
OrthoDB	EOG4STWDX.
Gene expression databases
ArrayExpress	Q00246.
Genevestigator	Q00246.
GermOnline	YKR055W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003578. Small_GTPase_Rho. [Graphical view]
KO	K07975.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975294.

Entry information

Entry name

RHO4_YEAST

Accession

Primary (citable) accession number: Q00246
Secondary accession number(s): D6VXB6, P30618

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	February 1, 1994
Last modified:	December 14, 2011
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents