Reviewed, UniProtKB/Swiss-Prot Q00246 (RHO4_YEAST)

Names and origin

Protein names

Recommended name:
    GTP-binding protein RHO4

Gene names

Name:	RHO4
Ordered Locus Names:	YKR055W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays an important role in cell growth. Required to keep the uninucleated state. May be involved in the organization of the cytoskeleton which affects microtubule functions. Most likely RHO3 and RHO4 of S.cerevisiae regulate partially overlapping but different pathways. Ref.6
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family.
Sequence caution	The sequence CAA78500.1 differs from that shown. Reason: Frameshift at position 263. Leads to a fusion with RNC1.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	actin filament organization Traceable author statement. Source: SGD establishment of cell polarity Traceable author statement. Source: SGD small GTPase mediated signal transduction Traceable author statement. Source: SGD
Cellular component	intracellular Traceable author statement. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Traceable author statement. Source: SGD protein binding Inferred from physical interaction. Source: IntAct signal transducer activity Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SSU81	P40073	1	EBI-15144,EBI-18140
URA1	P28272	1	EBI-15144,EBI-14386

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	GTP-binding protein RHO4		PRO_0000198948
	Propeptide	289 – 291	3	Removed in mature form By similarity		PRO_0000281278
Regions
	Nucleotide binding	79 – 86	8	GTP By similarity
	Nucleotide binding	127 – 131	5	GTP By similarity
	Nucleotide binding	185 – 188	4	GTP By similarity
	Motif	101 – 109	9	Effector region By similarity
Amino acid modifications
	Modified residue	47	1	Phosphothreonine Ref.7
	Modified residue	58	1	Phosphoserine Ref.7
	Modified residue	59	1	Phosphotyrosine Ref.7
	Modified residue	276	1	Phosphoserine Ref.7
	Modified residue	278	1	Phosphoserine Ref.7
	Modified residue	288	1	Cysteine methyl ester By similarity
	Lipidation	288	1	S-farnesyl cysteine By similarity
Experimental info
	Sequence conflict	43 – 48	6	PRLPTP → QIAYS in CAA78500. Ref.3
	Sequence conflict	143	1	T → R in CAA78500. Ref.3
	Sequence conflict	201 – 211	11	PSSAESLAKRL → QVQQNPWPSVW AA sequence Ref.3
	Sequence conflict	213 – 218	6	AFAHIQ → HLHIFK AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q00246-1 [UniParc]. Last modified February 1, 1994. Version 2. Checksum: 4B7BA75EA9BA50FD	FASTA	291	32,186
	10 20 30 40 50 60 MNTLLFKRKG GNCGNESNIV SQGSPSSSNL PESPGTLDEK NLPRLPTPFA RSLSTIPSYE 70 80 90 100 110 120 QMKRTNKLPD YHLKIVVVGD GAVGKTCLLI SYVQGTFPTD YIPTIFENYV TNIEGPNGQI 130 140 150 160 170 180 IELALWDTAG QEEYSRLRPL SYTNADVLMV CYSVGSKTSL KNVEDLWFPE VKHFCPSTPI 190 200 210 220 230 240 MLVGLKSDLY EADNLSDLVE PSSAESLAKR LGAFAHIQCS ARLKENIDEV FETAIHTLLS 250 260 270 280 290 DSLYAPREPT HTIKNPFKRN TTRSDIDSST GDTSVSISGT KRLRKNKCII M

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The RHO4a and NUD1 genes on Saccharomyces cerevisiae chromosome XI." van Vliet-Reedijk J.C., Planta R.J. Yeast 9:1139-1147(1993) [PubMed: 8256523] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Isolation and characterization of two novel ras superfamily genes in Saccharomyces cerevisiae." Matsui Y., Toh-e A. Gene 114:43-49(1992) [PubMed: 1587484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 62-291. Strain: ATCC 38626 / AH22 / NRRL Y-12843.
[3]	"Yeast RNC1 encodes a chimeric protein, RhoNUC, with a human rho motif and deoxyribonuclease activity." Chow T.Y.-K., Perkins E.L., Resnick M.A. Nucleic Acids Res. 20:5215-5221(1992) [PubMed: 1408836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 38-42.
[4]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1." Matsui Y., Toh-e A. Mol. Cell. Biol. 12:5690-5699(1992) [PubMed: 1448099] [Abstract] Cited for: FUNCTION.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-47; SER-58; TYR-59; SER-276 AND SER-278, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z25734 Genomic DNA. Translation: CAA81020.1. D10007 Genomic DNA. Translation: BAA00898.1. Z14126 Genomic DNA. Translation: CAA78500.1. Frameshift. Z28280 Genomic DNA. Translation: CAA82133.1. AY557909 Genomic DNA. Translation: AAS56235.1.
PIR	S37743.
RefSeq	NP_012981.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-755N.
IntAct	Q00246. 7 interactions.
STRING	Q00246.
Proteomic databases
PeptideAtlas	Q00246.
Genome annotation databases
Ensembl	YKR055W; YKR055W; YKR055W; Saccharomyces cerevisiae. [Genome view]
GeneID	853929.
KEGG	sce:YKR055W.
NMPDR	fig|4932.3.peg.3967.
Organism-specific databases
CYGD	YKR055w.
SGD	S000001763. RHO4.
Phylogenomic databases
HOGENOM	HBG745225.
OMA	VTNIEGP.
OrthoDB	EOG9VX3NN.
Gene expression databases
ArrayExpress	Q00246.
Genevestigator	Q00246.
GermOnline	YKR055W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003578. GTPase_Rho. IPR013753. Ras. IPR001806. Ras_GTPase. IPR005225. Small_GTP_bd. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	975294.

Entry information

Entry name

RHO4_YEAST

Accession

Primary (citable) accession number: Q00246
Secondary accession number(s): P30618

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	February 1, 1994
Last modified:	December 15, 2009
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)