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Q00246

- RHO4_YEAST

UniProt

Q00246 - RHO4_YEAST

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Protein

GTP-binding protein RHO4

Gene

RHO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays an important role in cell growth. Required to keep the uninucleated state. May be involved in the organization of the cytoskeleton which affects microtubule functions. Most likely RHO3 and RHO4 of S.cerevisiae regulate partially overlapping but different pathways.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi79 – 868GTPBy similarity
Nucleotide bindingi127 – 1315GTPBy similarity
Nucleotide bindingi185 – 1884GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: SGD
  2. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. GTP catabolic process Source: GOC
  2. maintenance of cell polarity Source: SGD
  3. positive regulation of formin-nucleated actin cable assembly Source: SGD
  4. regulation of formin-nucleated actin cable assembly Source: SGD
  5. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32024-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein RHO4
Gene namesi
Name:RHO4
Ordered Locus Names:YKR055W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKR055w.
SGDiS000001763. RHO4.

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. incipient cellular bud site Source: SGD
  3. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288GTP-binding protein RHO4PRO_0000198948Add
BLAST
Propeptidei289 – 2913Removed in mature formBy similarityPRO_0000281278

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei264 – 2641Phosphoserine1 Publication
Modified residuei268 – 2681Phosphoserine1 Publication
Modified residuei276 – 2761Phosphoserine2 Publications
Modified residuei288 – 2881Cysteine methyl esterBy similarity
Lipidationi288 – 2881S-farnesyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ00246.
PaxDbiQ00246.
PeptideAtlasiQ00246.

Expressioni

Gene expression databases

GenevestigatoriQ00246.

Interactioni

Protein-protein interaction databases

BioGridi34186. 69 interactions.
DIPiDIP-755N.
IntActiQ00246. 3 interactions.
MINTiMINT-390167.

Structurei

3D structure databases

ProteinModelPortaliQ00246.
SMRiQ00246. Positions 74-234.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi101 – 1099Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000119607.
HOGENOMiHOG000233974.
InParanoidiQ00246.
KOiK07975.
OMAiVTNIEGP.
OrthoDBiEOG7SN8QP.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00246-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTLLFKRKG GNCGNESNIV SQGSPSSSNL PESPGTLDEK NLPRLPTPFA
60 70 80 90 100
RSLSTIPSYE QMKRTNKLPD YHLKIVVVGD GAVGKTCLLI SYVQGTFPTD
110 120 130 140 150
YIPTIFENYV TNIEGPNGQI IELALWDTAG QEEYSRLRPL SYTNADVLMV
160 170 180 190 200
CYSVGSKTSL KNVEDLWFPE VKHFCPSTPI MLVGLKSDLY EADNLSDLVE
210 220 230 240 250
PSSAESLAKR LGAFAHIQCS ARLKENIDEV FETAIHTLLS DSLYAPREPT
260 270 280 290
HTIKNPFKRN TTRSDIDSST GDTSVSISGT KRLRKNKCII M
Length:291
Mass (Da):32,186
Last modified:February 1, 1994 - v2
Checksum:i4B7BA75EA9BA50FD
GO

Sequence cautioni

The sequence CAA78500.1 differs from that shown. Reason: Frameshift at position 263. Leads to a fusion with RNC1.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 486PRLPTP → QIAYS in CAA78500. (PubMed:1408836)Curated
Sequence conflicti143 – 1431T → R in CAA78500. (PubMed:1408836)Curated
Sequence conflicti201 – 21111PSSAESLAKRL → QVQQNPWPSVW AA sequence (PubMed:1408836)CuratedAdd
BLAST
Sequence conflicti213 – 2186AFAHIQ → HLHIFK AA sequence (PubMed:1408836)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z25734 Genomic DNA. Translation: CAA81020.1.
D10007 Genomic DNA. Translation: BAA00898.1.
Z14126 Genomic DNA. Translation: CAA78500.1. Frameshift.
Z28280 Genomic DNA. Translation: CAA82133.1.
AY557909 Genomic DNA. Translation: AAS56235.1.
BK006944 Genomic DNA. Translation: DAA09206.1.
PIRiS37743.
RefSeqiNP_012981.3. NM_001179845.3.

Genome annotation databases

EnsemblFungiiYKR055W; YKR055W; YKR055W.
GeneIDi853929.
KEGGisce:YKR055W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z25734 Genomic DNA. Translation: CAA81020.1 .
D10007 Genomic DNA. Translation: BAA00898.1 .
Z14126 Genomic DNA. Translation: CAA78500.1 . Frameshift.
Z28280 Genomic DNA. Translation: CAA82133.1 .
AY557909 Genomic DNA. Translation: AAS56235.1 .
BK006944 Genomic DNA. Translation: DAA09206.1 .
PIRi S37743.
RefSeqi NP_012981.3. NM_001179845.3.

3D structure databases

ProteinModelPortali Q00246.
SMRi Q00246. Positions 74-234.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34186. 69 interactions.
DIPi DIP-755N.
IntActi Q00246. 3 interactions.
MINTi MINT-390167.

Proteomic databases

MaxQBi Q00246.
PaxDbi Q00246.
PeptideAtlasi Q00246.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKR055W ; YKR055W ; YKR055W .
GeneIDi 853929.
KEGGi sce:YKR055W.

Organism-specific databases

CYGDi YKR055w.
SGDi S000001763. RHO4.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00760000119607.
HOGENOMi HOG000233974.
InParanoidi Q00246.
KOi K07975.
OMAi VTNIEGP.
OrthoDBi EOG7SN8QP.

Enzyme and pathway databases

BioCyci YEAST:G3O-32024-MONOMER.

Miscellaneous databases

NextBioi 975294.

Gene expression databases

Genevestigatori Q00246.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00174. RHO. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51420. RHO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The RHO4a and NUD1 genes on Saccharomyces cerevisiae chromosome XI."
    van Vliet-Reedijk J.C., Planta R.J.
    Yeast 9:1139-1147(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Isolation and characterization of two novel ras superfamily genes in Saccharomyces cerevisiae."
    Matsui Y., Toh-E A.
    Gene 114:43-49(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 62-291.
    Strain: ATCC 38626 / AH22 / NRRL Y-12843.
  3. "Yeast RNC1 encodes a chimeric protein, RhoNUC, with a human rho motif and deoxyribonuclease activity."
    Chow T.Y.-K., Perkins E.L., Resnick M.A.
    Nucleic Acids Res. 20:5215-5221(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 38-42.
  4. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1."
    Matsui Y., Toh-E A.
    Mol. Cell. Biol. 12:5690-5699(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264; SER-268 AND SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRHO4_YEAST
AccessioniPrimary (citable) accession number: Q00246
Secondary accession number(s): D6VXB6, P30618
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3