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Q00245

- RHO3_YEAST

UniProt

Q00245 - RHO3_YEAST

Protein

GTP-binding protein RHO3

Gene

RHO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Plays an important role in cell growth. Required to keep the uninucleated state. May be involved in the organization of the cytoskeleton which affects microtubule functions. Most likely RHO3 and RHO4 of S.cerevisiae regulate partially overlapping but different pathways.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi23 – 308GTPBy similarity
    Nucleotide bindingi70 – 745GTPBy similarity
    Nucleotide bindingi128 – 1314GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. establishment or maintenance of actin cytoskeleton polarity Source: SGD
    2. GTP catabolic process Source: GOC
    3. positive regulation of exocytosis Source: SGD
    4. positive regulation of formin-nucleated actin cable assembly Source: SGD
    5. small GTPase mediated signal transduction Source: InterPro

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31371-MONOMER.
    ReactomeiREACT_188387. Axonal growth inhibition (RHOA activation).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP-binding protein RHO3
    Gene namesi
    Name:RHO3
    Ordered Locus Names:YIL118W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL118w.
    SGDiS000001380. RHO3.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. cellular bud Source: SGD
    2. cytosol Source: SGD
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 228228GTP-binding protein RHO3PRO_0000198947Add
    BLAST
    Propeptidei229 – 2313Removed in mature formBy similarityPRO_0000281277

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei228 – 2281Cysteine methyl esterBy similarity
    Lipidationi228 – 2281S-farnesyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    MaxQBiQ00245.
    PaxDbiQ00245.
    PeptideAtlasiQ00245.

    Expressioni

    Gene expression databases

    GenevestigatoriQ00245.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EXO70P196583EBI-15138,EBI-6717

    Protein-protein interaction databases

    BioGridi34873. 63 interactions.
    DIPiDIP-4820N.
    IntActiQ00245. 1 interaction.
    MINTiMINT-482205.
    STRINGi4932.YIL118W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00245.
    SMRiQ00245. Positions 15-213.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi45 – 539Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rho family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00750000118005.
    HOGENOMiHOG000233974.
    KOiK07975.
    OMAiYFPTVYE.
    OrthoDBiEOG7SN8QP.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00174. RHO. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51420. RHO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q00245-1 [UniParc]FASTAAdd to Basket

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    MSFLCGSAST SNKPIERKIV ILGDGACGKT SLLNVFTRGY FPEVYEPTVF    50
    ENYIHDIFVD SKHITLSLWD TAGQEEFDRL RSLSYSDTQC IMLCFSIDSR 100
    DSLENVQNKW VGEITDHCEG VKLVLVALKC DLRNNENESN AITPNNIQQD 150
    NSVSNDNGNN INSTSNGKNL ISYEEGLAMA KKIGALRYLE CSAKLNKGVN 200
    EAFTEAARVA LTAGPVATEV KSDSGSSCTI M 231
    Length:231
    Mass (Da):25,312
    Last modified:February 1, 1995 - v2
    Checksum:i7C8BEB15502AD024
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti129 – 1291K → E in BAA00897. (PubMed:1587484)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10006 Genomic DNA. Translation: BAA00897.1.
    Z46833 Genomic DNA. Translation: CAA86874.1.
    AY557857 Genomic DNA. Translation: AAS56183.1.
    BK006942 Genomic DNA. Translation: DAA08435.1.
    PIRiS49891.
    RefSeqiNP_012148.1. NM_001179466.1.

    Genome annotation databases

    EnsemblFungiiYIL118W; YIL118W; YIL118W.
    GeneIDi854688.
    KEGGisce:YIL118W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10006 Genomic DNA. Translation: BAA00897.1 .
    Z46833 Genomic DNA. Translation: CAA86874.1 .
    AY557857 Genomic DNA. Translation: AAS56183.1 .
    BK006942 Genomic DNA. Translation: DAA08435.1 .
    PIRi S49891.
    RefSeqi NP_012148.1. NM_001179466.1.

    3D structure databases

    ProteinModelPortali Q00245.
    SMRi Q00245. Positions 15-213.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34873. 63 interactions.
    DIPi DIP-4820N.
    IntActi Q00245. 1 interaction.
    MINTi MINT-482205.
    STRINGi 4932.YIL118W.

    Proteomic databases

    MaxQBi Q00245.
    PaxDbi Q00245.
    PeptideAtlasi Q00245.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL118W ; YIL118W ; YIL118W .
    GeneIDi 854688.
    KEGGi sce:YIL118W.

    Organism-specific databases

    CYGDi YIL118w.
    SGDi S000001380. RHO3.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00750000118005.
    HOGENOMi HOG000233974.
    KOi K07975.
    OMAi YFPTVYE.
    OrthoDBi EOG7SN8QP.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31371-MONOMER.
    Reactomei REACT_188387. Axonal growth inhibition (RHOA activation).

    Miscellaneous databases

    NextBioi 977310.
    PROi Q00245.

    Gene expression databases

    Genevestigatori Q00245.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00174. RHO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51420. RHO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of two novel ras superfamily genes in Saccharomyces cerevisiae."
      Matsui Y., Toh-E A.
      Gene 114:43-49(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 38626 / AH22 / NRRL Y-12843.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1."
      Matsui Y., Toh-E A.
      Mol. Cell. Biol. 12:5690-5699(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRHO3_YEAST
    AccessioniPrimary (citable) accession number: Q00245
    Secondary accession number(s): D6VVG9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5910 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3