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Q00245

- RHO3_YEAST

UniProt

Q00245 - RHO3_YEAST

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Protein

GTP-binding protein RHO3

Gene

RHO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays an important role in cell growth. Required to keep the uninucleated state. May be involved in the organization of the cytoskeleton which affects microtubule functions. Most likely RHO3 and RHO4 of S.cerevisiae regulate partially overlapping but different pathways.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi23 – 308GTPBy similarity
Nucleotide bindingi70 – 745GTPBy similarity
Nucleotide bindingi128 – 1314GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: SGD
  2. GTP binding Source: SGD

GO - Biological processi

  1. establishment or maintenance of actin cytoskeleton polarity Source: SGD
  2. GTP catabolic process Source: GOC
  3. positive regulation of exocytosis Source: SGD
  4. positive regulation of formin-nucleated actin cable assembly Source: SGD
  5. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31371-MONOMER.
ReactomeiREACT_230996. VEGFA-VEGFR2 Pathway.
REACT_240239. Rho GTPase cycle.
REACT_246186. G beta:gamma signalling through PI3Kgamma.
REACT_248666. G alpha (12/13) signalling events.
REACT_254325. PI3K/AKT activation.
REACT_269225. EPHA-mediated growth cone collapse.
REACT_269379. Sema4D induced cell migration and growth-cone collapse.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein RHO3
Gene namesi
Name:RHO3
Ordered Locus Names:YIL118W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL118w.
SGDiS000001380. RHO3.

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

GO - Cellular componenti

  1. cellular bud Source: SGD
  2. cytosol Source: SGD
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228GTP-binding protein RHO3PRO_0000198947Add
BLAST
Propeptidei229 – 2313Removed in mature formBy similarityPRO_0000281277

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei228 – 2281Cysteine methyl esterBy similarity
Lipidationi228 – 2281S-farnesyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiQ00245.
PaxDbiQ00245.
PeptideAtlasiQ00245.

Expressioni

Gene expression databases

GenevestigatoriQ00245.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
EXO70P196583EBI-15138,EBI-6717

Protein-protein interaction databases

BioGridi34873. 64 interactions.
DIPiDIP-4820N.
IntActiQ00245. 1 interaction.
MINTiMINT-482205.
STRINGi4932.YIL118W.

Structurei

3D structure databases

ProteinModelPortaliQ00245.
SMRiQ00245. Positions 15-213.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi45 – 539Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000119850.
HOGENOMiHOG000233974.
InParanoidiQ00245.
KOiK07975.
OMAiYFPTVYE.
OrthoDBiEOG7SN8QP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00245-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFLCGSAST SNKPIERKIV ILGDGACGKT SLLNVFTRGY FPEVYEPTVF
60 70 80 90 100
ENYIHDIFVD SKHITLSLWD TAGQEEFDRL RSLSYSDTQC IMLCFSIDSR
110 120 130 140 150
DSLENVQNKW VGEITDHCEG VKLVLVALKC DLRNNENESN AITPNNIQQD
160 170 180 190 200
NSVSNDNGNN INSTSNGKNL ISYEEGLAMA KKIGALRYLE CSAKLNKGVN
210 220 230
EAFTEAARVA LTAGPVATEV KSDSGSSCTI M
Length:231
Mass (Da):25,312
Last modified:February 1, 1995 - v2
Checksum:i7C8BEB15502AD024
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1291K → E in BAA00897. (PubMed:1587484)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10006 Genomic DNA. Translation: BAA00897.1.
Z46833 Genomic DNA. Translation: CAA86874.1.
AY557857 Genomic DNA. Translation: AAS56183.1.
BK006942 Genomic DNA. Translation: DAA08435.1.
PIRiS49891.
RefSeqiNP_012148.1. NM_001179466.1.

Genome annotation databases

EnsemblFungiiYIL118W; YIL118W; YIL118W.
GeneIDi854688.
KEGGisce:YIL118W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10006 Genomic DNA. Translation: BAA00897.1 .
Z46833 Genomic DNA. Translation: CAA86874.1 .
AY557857 Genomic DNA. Translation: AAS56183.1 .
BK006942 Genomic DNA. Translation: DAA08435.1 .
PIRi S49891.
RefSeqi NP_012148.1. NM_001179466.1.

3D structure databases

ProteinModelPortali Q00245.
SMRi Q00245. Positions 15-213.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34873. 64 interactions.
DIPi DIP-4820N.
IntActi Q00245. 1 interaction.
MINTi MINT-482205.
STRINGi 4932.YIL118W.

Proteomic databases

MaxQBi Q00245.
PaxDbi Q00245.
PeptideAtlasi Q00245.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL118W ; YIL118W ; YIL118W .
GeneIDi 854688.
KEGGi sce:YIL118W.

Organism-specific databases

CYGDi YIL118w.
SGDi S000001380. RHO3.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00760000119850.
HOGENOMi HOG000233974.
InParanoidi Q00245.
KOi K07975.
OMAi YFPTVYE.
OrthoDBi EOG7SN8QP.

Enzyme and pathway databases

BioCyci YEAST:G3O-31371-MONOMER.
Reactomei REACT_230996. VEGFA-VEGFR2 Pathway.
REACT_240239. Rho GTPase cycle.
REACT_246186. G beta:gamma signalling through PI3Kgamma.
REACT_248666. G alpha (12/13) signalling events.
REACT_254325. PI3K/AKT activation.
REACT_269225. EPHA-mediated growth cone collapse.
REACT_269379. Sema4D induced cell migration and growth-cone collapse.

Miscellaneous databases

NextBioi 977310.
PROi Q00245.

Gene expression databases

Genevestigatori Q00245.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00174. RHO. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51420. RHO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of two novel ras superfamily genes in Saccharomyces cerevisiae."
    Matsui Y., Toh-E A.
    Gene 114:43-49(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 38626 / AH22 / NRRL Y-12843.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1."
    Matsui Y., Toh-E A.
    Mol. Cell. Biol. 12:5690-5699(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRHO3_YEAST
AccessioniPrimary (citable) accession number: Q00245
Secondary accession number(s): D6VVG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1995
Last modified: November 26, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5910 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3