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Protein

GTP-binding protein RHO3

Gene

RHO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in cell growth. Required to keep the uninucleated state. May be involved in the organization of the cytoskeleton which affects microtubule functions. Most likely RHO3 and RHO4 of S.cerevisiae regulate partially overlapping but different pathways.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi23 – 30GTPBy similarity8
Nucleotide bindingi70 – 74GTPBy similarity5
Nucleotide bindingi128 – 131GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: SGD

GO - Biological processi

  • establishment or maintenance of actin cytoskeleton polarity Source: SGD
  • positive regulation of exocytosis Source: SGD
  • positive regulation of formin-nucleated actin cable assembly Source: SGD
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31371-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein RHO3
Gene namesi
Name:RHO3
Ordered Locus Names:YIL118W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL118W.
SGDiS000001380. RHO3.

Subcellular locationi

GO - Cellular componenti

  • cellular bud Source: SGD
  • cytosol Source: SGD
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001989471 – 228GTP-binding protein RHO3Add BLAST228
PropeptideiPRO_0000281277229 – 231Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei228Cysteine methyl esterBy similarity1
Lipidationi228S-farnesyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiQ00245.
PRIDEiQ00245.

PTM databases

SwissPalmiQ00245.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
EXO70P196583EBI-15138,EBI-6717

Protein-protein interaction databases

BioGridi34873. 63 interactors.
DIPiDIP-4820N.
IntActiQ00245. 1 interactor.
MINTiMINT-482205.

Structurei

3D structure databases

ProteinModelPortaliQ00245.
SMRiQ00245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi45 – 53Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

GeneTreeiENSGT00830000129187.
HOGENOMiHOG000233974.
InParanoidiQ00245.
KOiK07975.
OMAiNVHIELS.
OrthoDBiEOG092C4TDG.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00245-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFLCGSAST SNKPIERKIV ILGDGACGKT SLLNVFTRGY FPEVYEPTVF
60 70 80 90 100
ENYIHDIFVD SKHITLSLWD TAGQEEFDRL RSLSYSDTQC IMLCFSIDSR
110 120 130 140 150
DSLENVQNKW VGEITDHCEG VKLVLVALKC DLRNNENESN AITPNNIQQD
160 170 180 190 200
NSVSNDNGNN INSTSNGKNL ISYEEGLAMA KKIGALRYLE CSAKLNKGVN
210 220 230
EAFTEAARVA LTAGPVATEV KSDSGSSCTI M
Length:231
Mass (Da):25,312
Last modified:February 1, 1995 - v2
Checksum:i7C8BEB15502AD024
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti129K → E in BAA00897 (PubMed:1587484).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10006 Genomic DNA. Translation: BAA00897.1.
Z46833 Genomic DNA. Translation: CAA86874.1.
AY557857 Genomic DNA. Translation: AAS56183.1.
BK006942 Genomic DNA. Translation: DAA08435.1.
PIRiS49891.
RefSeqiNP_012148.1. NM_001179466.1.

Genome annotation databases

EnsemblFungiiYIL118W; YIL118W; YIL118W.
GeneIDi854688.
KEGGisce:YIL118W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10006 Genomic DNA. Translation: BAA00897.1.
Z46833 Genomic DNA. Translation: CAA86874.1.
AY557857 Genomic DNA. Translation: AAS56183.1.
BK006942 Genomic DNA. Translation: DAA08435.1.
PIRiS49891.
RefSeqiNP_012148.1. NM_001179466.1.

3D structure databases

ProteinModelPortaliQ00245.
SMRiQ00245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34873. 63 interactors.
DIPiDIP-4820N.
IntActiQ00245. 1 interactor.
MINTiMINT-482205.

PTM databases

SwissPalmiQ00245.

Proteomic databases

MaxQBiQ00245.
PRIDEiQ00245.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL118W; YIL118W; YIL118W.
GeneIDi854688.
KEGGisce:YIL118W.

Organism-specific databases

EuPathDBiFungiDB:YIL118W.
SGDiS000001380. RHO3.

Phylogenomic databases

GeneTreeiENSGT00830000129187.
HOGENOMiHOG000233974.
InParanoidiQ00245.
KOiK07975.
OMAiNVHIELS.
OrthoDBiEOG092C4TDG.

Enzyme and pathway databases

BioCyciYEAST:G3O-31371-MONOMER.

Miscellaneous databases

PROiQ00245.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHO3_YEAST
AccessioniPrimary (citable) accession number: Q00245
Secondary accession number(s): D6VVG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5910 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.