Q00245 (RHO3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP-binding protein RHO3

Gene names

Name:	RHO3
Ordered Locus Names:	YIL118W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in cell growth. Required to keep the uninucleated state. May be involved in the organization of the cytoskeleton which affects microtubule functions. Most likely RHO3 and RHO4 of S.cerevisiae regulate partially overlapping but different pathways. Ref.5
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Miscellaneous	Present with 5910 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	establishment or maintenance of actin cytoskeleton polarity Inferred from mutant phenotype PubMed 10588647PubMed 8852836. Source: SGD positive regulation of exocytosis Inferred from mutant phenotype PubMed 10588647. Source: SGD positive regulation of formin-nucleated actin cable assembly Inferred from mutant phenotype PubMed 12810699. Source: SGD small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cellular bud Inferred from direct assay PubMed 10207081. Source: SGD cytosol Inferred from direct assay PubMed 10207081. Source: SGD plasma membrane Inferred from direct assay PubMed 16622836. Source: SGD
Molecular_function	GTP binding Inferred from direct assay PubMed 10207081. Source: SGD GTPase activity Inferred from direct assay PubMed 10526184. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	GTP-binding protein RHO3		PRO_0000198947
	Propeptide	229 – 231	3	Removed in mature form By similarity		PRO_0000281277
Regions
	Nucleotide binding	23 – 30	8	GTP By similarity
	Nucleotide binding	70 – 74	5	GTP By similarity
	Nucleotide binding	128 – 131	4	GTP By similarity
	Motif	45 – 53	9	Effector region By similarity
Amino acid modifications
	Modified residue	228	1	Cysteine methyl ester By similarity
	Lipidation	228	1	S-farnesyl cysteine By similarity
Experimental info
	Sequence conflict	129	1	K → E in BAA00897. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q00245 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 7C8BEB15502AD024	FASTA	231	25,312
	10 20 30 40 50 60 MSFLCGSAST SNKPIERKIV ILGDGACGKT SLLNVFTRGY FPEVYEPTVF ENYIHDIFVD 70 80 90 100 110 120 SKHITLSLWD TAGQEEFDRL RSLSYSDTQC IMLCFSIDSR DSLENVQNKW VGEITDHCEG 130 140 150 160 170 180 VKLVLVALKC DLRNNENESN AITPNNIQQD NSVSNDNGNN INSTSNGKNL ISYEEGLAMA 190 200 210 220 230 KKIGALRYLE CSAKLNKGVN EAFTEAARVA LTAGPVATEV KSDSGSSCTI M

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of two novel ras superfamily genes in Saccharomyces cerevisiae." Matsui Y., Toh-E A. Gene 114:43-49(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38626 / AH22 / NRRL Y-12843.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1." Matsui Y., Toh-E A. Mol. Cell. Biol. 12:5690-5699(1992) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10006 Genomic DNA. Translation: BAA00897.1. Z46833 Genomic DNA. Translation: CAA86874.1. AY557857 Genomic DNA. Translation: AAS56183.1. BK006942 Genomic DNA. Translation: DAA08435.1.
PIR	S49891.
RefSeq	NP_012148.1. NM_001179466.1.
3D structure databases
ProteinModelPortal	Q00245.
SMR	Q00245. Positions 15-213.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4820N.
MINT	MINT-482205.
STRING	4932.YIL118W.
Proteomic databases
PaxDb	Q00245.
PeptideAtlas	Q00245.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL118W; YIL118W; YIL118W.
GeneID	854688.
KEGG	sce:YIL118W.
Organism-specific databases
CYGD	YIL118w.
SGD	S000001380. RHO3.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00680000100556.
HOGENOM	HOG000233974.
KO	K07975.
OMA	VFENYVH.
OrthoDB	EOG4ZW8M9.
Gene expression databases
Genevestigator	Q00245.
GermOnline	YIL118W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003578. Small_GTPase_Rho. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977310.

Entry information

Entry name

RHO3_YEAST

Accession

Primary (citable) accession number: Q00245
Secondary accession number(s): D6VVG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program