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Q00245 (RHO3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP-binding protein RHO3
Gene names
Name:RHO3
Ordered Locus Names:YIL118W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length231 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in cell growth. Required to keep the uninucleated state. May be involved in the organization of the cytoskeleton which affects microtubule functions. Most likely RHO3 and RHO4 of S.cerevisiae regulate partially overlapping but different pathways. Ref.5

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

Miscellaneous

Present with 5910 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the small GTPase superfamily. Rho family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

EXO70P196583EBI-15138,EBI-6717

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 228228GTP-binding protein RHO3
PRO_0000198947
Propeptide229 – 2313Removed in mature form By similarity
PRO_0000281277

Regions

Nucleotide binding23 – 308GTP By similarity
Nucleotide binding70 – 745GTP By similarity
Nucleotide binding128 – 1314GTP By similarity
Motif45 – 539Effector region By similarity

Amino acid modifications

Modified residue2281Cysteine methyl ester By similarity
Lipidation2281S-farnesyl cysteine By similarity

Experimental info

Sequence conflict1291K → E in BAA00897. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q00245 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 7C8BEB15502AD024

FASTA23125,312
        10         20         30         40         50         60 
MSFLCGSAST SNKPIERKIV ILGDGACGKT SLLNVFTRGY FPEVYEPTVF ENYIHDIFVD 

        70         80         90        100        110        120 
SKHITLSLWD TAGQEEFDRL RSLSYSDTQC IMLCFSIDSR DSLENVQNKW VGEITDHCEG 

       130        140        150        160        170        180 
VKLVLVALKC DLRNNENESN AITPNNIQQD NSVSNDNGNN INSTSNGKNL ISYEEGLAMA 

       190        200        210        220        230 
KKIGALRYLE CSAKLNKGVN EAFTEAARVA LTAGPVATEV KSDSGSSCTI M 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of two novel ras superfamily genes in Saccharomyces cerevisiae."
Matsui Y., Toh-E A.
Gene 114:43-49(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 38626 / AH22 / NRRL Y-12843.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1."
Matsui Y., Toh-E A.
Mol. Cell. Biol. 12:5690-5699(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10006 Genomic DNA. Translation: BAA00897.1.
Z46833 Genomic DNA. Translation: CAA86874.1.
AY557857 Genomic DNA. Translation: AAS56183.1.
BK006942 Genomic DNA. Translation: DAA08435.1.
PIRS49891.
RefSeqNP_012148.1. NM_001179466.1.

3D structure databases

ProteinModelPortalQ00245.
SMRQ00245. Positions 15-213.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34873. 63 interactions.
DIPDIP-4820N.
IntActQ00245. 1 interaction.
MINTMINT-482205.
STRING4932.YIL118W.

Proteomic databases

PaxDbQ00245.
PeptideAtlasQ00245.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL118W; YIL118W; YIL118W.
GeneID854688.
KEGGsce:YIL118W.

Organism-specific databases

CYGDYIL118w.
SGDS000001380. RHO3.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00750000118005.
HOGENOMHOG000233974.
KOK07975.
OMAYFPTVYE.
OrthoDBEOG7SN8QP.

Enzyme and pathway databases

BioCycYEAST:G3O-31371-MONOMER.

Gene expression databases

GenevestigatorQ00245.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977310.
PROQ00245.

Entry information

Entry nameRHO3_YEAST
AccessionPrimary (citable) accession number: Q00245
Secondary accession number(s): D6VVG9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families