Reviewed,
UniProtKB/Swiss-Prot Q00234 (TYRO_ASPOR)
Last modified
January 19, 2010.
Version 54.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Tyrosinase EC=1.14.18.1 Alternative name(s): Monophenol monooxygenase | ||||
| Gene names |
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| Organism | Aspergillus oryzae [Complete proteome] | ||||
| Taxonomic identifier | 5062 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus |
Protein attributes
| Sequence length | 539 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. |
| Catalytic activity | L-tyrosine + L-dopa + O2 = L-dopa + dopaquinone + H2O. |
| Cofactor | Binds 2 copper ions per subunit. |
| Enzyme regulation | Activated by acidifying treatment at pH 3.0. |
| Subunit structure | Homotetramer. |
| Post-translational modification | The N-terminus is blocked. |
| Sequence similarities | Belongs to the tyrosinase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Melanin biosynthesis |
| Ligand | Copper Metal-binding |
| Molecular function | Monooxygenase Oxidoreductase |
| PTM | Thioether bond |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | melanin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW monophenol monooxygenase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 539 | 539 | Tyrosinase | PRO_0000186733 | |||||||
Sites | |||||||||||
| Metal binding | 63 | 1 | Copper A By similarity | ||||||||
| Metal binding | 84 | 1 | Copper A By similarity | ||||||||
| Metal binding | 93 | 1 | Copper A By similarity | ||||||||
| Metal binding | 290 | 1 | Copper B By similarity | ||||||||
| Metal binding | 294 | 1 | Copper B By similarity | ||||||||
| Metal binding | 333 | 1 | Copper B By similarity | ||||||||
Amino acid modifications | |||||||||||
| Cross-link | 82 ↔ 84 | 2'-(S-cysteinyl)-histidine (Cys-His) By similarity | |||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning and nucleotide sequence of the protyrosinase gene, melO, from Aspergillus oryzae and expression of the gene in yeast cells." Fujita Y., Uraga Y., Ichishima E. Biochim. Biophys. Acta 1261:151-154(1995) [PubMed: 7893753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 22788 / RIB 128 / CBS 819.72 / IFO 30113 / JCM 2248. |
| [2] | "Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. Kikuchi H.Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D37929 Genomic DNA. Translation: BAA07149.1. AP007169 Genomic DNA. Translation: BAE63910.1. |
| PIR | S53529. |
| RefSeq | XP_001825043.1. |
3D structure databases | |
| SMR | Q00234. Positions 23-417. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 5997138. |
| GenomeReviews | Gene locus melO in contig AP007169_GR. |
| KEGG | aor:AO090038000061. |
Phylogenomic databases | |
| OrthoDB | EOG9PP00S. |
Enzyme and pathway databases | |
| BRENDA | 1.14.18.1. 2240. |
Family and domain databases | |
| InterPro | IPR008922. Di-copper_centre. IPR016216. Monophenol_mOase_fun. IPR002227. Tyrosinase. [Graphical view] |
| Gene3D | G3DSA:1.10.1280.10. Di-copper_centre. 1 hit. |
| Pfam | PF00264. Tyrosinase. 1 hit. [Graphical view] |
| PIRSF | PIRSF000340. MPO_fungal. 1 hit. |
| PRINTS | PR00092. TYROSINASE. |
| PROSITE | PS00497. TYROSINASE_1. 1 hit. PS00498. TYROSINASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | TYRO_ASPOR | ||||||||
| Accession | Primary (citable) accession number: Q00234 Secondary accession number(s): Q2U3F5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||

Clusters with


