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Protein

Tyrosinase

Gene

melO

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.

Catalytic activityi

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
L-tyrosine + O2 = dopaquinone + H2O.

Cofactori

Cu2+Note: Binds 2 copper ions per subunit.

Enzyme regulationi

Activated by acidifying treatment at pH 3.0.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi63 – 631Copper ABy similarity
Metal bindingi84 – 841Copper ABy similarity
Metal bindingi93 – 931Copper ABy similarity
Metal bindingi290 – 2901Copper BBy similarity
Metal bindingi294 – 2941Copper BBy similarity
Metal bindingi333 – 3331Copper BBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. monophenol monooxygenase activity Source: ASPGD

GO - Biological processi

  1. melanin biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosinase (EC:1.14.18.1)
Alternative name(s):
Monophenol monooxygenase
Gene namesi
Name:melO
ORF Names:AO090038000061
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006564 Componenti: Chromosome 6

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 539539TyrosinasePRO_0000186733Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki82 ↔ 842'-(S-cysteinyl)-histidine (Cys-His)By similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Thioether bond

Proteomic databases

PRIDEiQ00234.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi5062.CADAORAP00008707.

Structurei

3D structure databases

ProteinModelPortaliQ00234.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Curated

Phylogenomic databases

HOGENOMiHOG000250264.
OMAiPFRGAGY.
OrthoDBiEOG7JX3D0.

Family and domain databases

Gene3Di1.10.1280.10. 3 hits.
InterProiIPR016216. Monophenol_mOase_fun.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000340. MPO_fungal. 1 hit.
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 2 hits.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00234-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVEPIKTF EIRQKGPVET KAERKSIRDL NEEELDKLIE AWRWIQDPAR
60 70 80 90 100
TGEDSFFYLA GLHGEPFRGA GYNNSHWWGG YCHHGNILFP TWHRAYLMAV
110 120 130 140 150
EKALRKACPD VSLPYWDESD DETAKKGIPL IFTQKEYKGK PNPLYSYTFS
160 170 180 190 200
ERIVDRLAKF PDADYSKPQG YKTCRYPYSG LCGQDDIAIA QQHNNFLDAN
210 220 230 240 250
FNQEQITGLL NSNVTSWLNL GQFTDIEGKQ VKADTRWKIR QCLLTEEYTV
260 270 280 290 300
FSNTTSAQRW NDEQFHPLES GGKETEAKAT SLAVPLESPH NDMHLAIGGV
310 320 330 340 350
QIPGFNVDQY AGANGDMGEN DTASFDPIFY FHHCFIDYLF WTWQTMHKKT
360 370 380 390 400
DASQITILPE YPGTNSVDSQ GPTPGISGNT WLTLDTPLDP FRENGDKVTS
410 420 430 440 450
NKLLTLKDLP YTYKAPTSGT GSVFNDVPRL NYPLSPPILR VSGINRASIA
460 470 480 490 500
GSFALAISQT DHTGKAQVKG IESVLSRWHV QGCANCQTHL STTAFVPLFE
510 520 530
LNEDDAKRKH ANNELAVHLH TRGNPGGQRV RNVTVGTMR
Length:539
Mass (Da):60,605
Last modified:October 31, 1996 - v1
Checksum:iCD2ECD702A018E15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37929 Genomic DNA. Translation: BAA07149.1.
AP007169 Genomic DNA. Translation: BAE63910.1.
PIRiS53529.
RefSeqiXP_001825043.1. XM_001824991.2.

Genome annotation databases

EnsemblFungiiCADAORAT00008890; CADAORAP00008707; CADAORAG00008890.
GeneIDi5997138.
KEGGiaor:AOR_1_92074.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37929 Genomic DNA. Translation: BAA07149.1.
AP007169 Genomic DNA. Translation: BAE63910.1.
PIRiS53529.
RefSeqiXP_001825043.1. XM_001824991.2.

3D structure databases

ProteinModelPortaliQ00234.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5062.CADAORAP00008707.

Proteomic databases

PRIDEiQ00234.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAORAT00008890; CADAORAP00008707; CADAORAG00008890.
GeneIDi5997138.
KEGGiaor:AOR_1_92074.

Phylogenomic databases

HOGENOMiHOG000250264.
OMAiPFRGAGY.
OrthoDBiEOG7JX3D0.

Family and domain databases

Gene3Di1.10.1280.10. 3 hits.
InterProiIPR016216. Monophenol_mOase_fun.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000340. MPO_fungal. 1 hit.
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 2 hits.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and nucleotide sequence of the protyrosinase gene, melO, from Aspergillus oryzae and expression of the gene in yeast cells."
    Fujita Y., Uraga Y., Ichishima E.
    Biochim. Biophys. Acta 1261:151-154(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 22788 / RIB 128 / CBS 819.72 / IFO 30113 / JCM 2248.
  2. "Genome sequencing and analysis of Aspergillus oryzae."
    Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
    , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
    Nature 438:1157-1161(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 42149 / RIB 40.

Entry informationi

Entry nameiTYRO_ASPOR
AccessioniPrimary (citable) accession number: Q00234
Secondary accession number(s): Q2U3F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 14, 1998
Last sequence update: October 31, 1996
Last modified: January 6, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.