Q00218 (AROG_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase 2, chloroplastic
EC=2.5.1.54

Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 2
DAHP synthase 2
Phospho-2-keto-3-deoxyheptonate aldolase 2

Gene names

Name:	DHS2
Ordered Locus Names:	At4g33510
ORF Names:	F17M5.270

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the class-II DAHP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW chorismate biosynthetic process Inferred from genetic interaction Ref.1. Source: TAIR
Cellular component	chloroplast thylakoid Inferred from direct assay. Source: TAIR
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q00218-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 507		Phospho-2-dehydro-3-deoxyheptonate aldolase 2, chloroplastic		PRO_0000002299
Experimental info
	Sequence conflict	165	1	S → L in AAA32785. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: 8B58271053399AA1	FASTA	507	56,149
	10 20 30 40 50 60 MVTLNASSPL TTKSFLPYRH APRRPISFSP VFAVHSTDPK KSTQSASASV KWSLESWKSK 70 80 90 100 110 120 KALQLPDYPD QKDVDSVLQT LSSFPPIVFA GEARKLEDKL GQAAMGQAFM LQGGDCAESF 130 140 150 160 170 180 KEFNANNIRD TFRVLLQMGV VLMFGGQLPV IKVGRMAGQF AKPRSDPFEE KDGVKLPSYR 190 200 210 220 230 240 GDNINGDAFD EKSRIPDPHR MVRAYTQSVA TLNLLRAFAT GGYAAMQRVS QWNLDFTQHS 250 260 270 280 290 300 EQGDRYRELA NRVDEALGFM GAAGLTSAHP IMTTTEFWTS HECLLLPYEQ ALTREDSTSG 310 320 330 340 350 360 LYYDCSAHML WVGERTRQLD GAHVEFLRGI ANPLGIKVSD KMVPSELVKL IEILNPQNKP 370 380 390 400 410 420 GRITVIVRMG AENMRVKLPN LIRAVRGAGQ IVTWVSDPMH GNTIMAPGGL KTRSFDAIRA 430 440 450 460 470 480 ELRAFFDVHD QEGSFPGGVH LEMTGQNVTE CVGGSRTITY NDLSSRYHTH CDPRLNASQS 490 500 LELAFIIAER LRKRRLGSGN LPSSIGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential induction of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase genes in Arabidopsis thaliana by wounding and pathogenic attack." Keith B., Dong X.N., Ausubel F.M., Fink G.R. Proc. Natl. Acad. Sci. U.S.A. 88:8821-8825(1991) [PubMed: 1681544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M74820 mRNA. Translation: AAA32785.1. AL035678 Genomic DNA. Translation: CAB38809.1. AL161583 Genomic DNA. Translation: CAB80068.1. CP002687 Genomic DNA. Translation: AEE86237.1. AF361798 mRNA. Translation: AAK32811.1. AY124858 mRNA. Translation: AAM70567.1.
IPI	IPI00530558.
PIR	T06002.
RefSeq	NP_195077.1. NM_119505.2.
UniGene	At.301.
3D structure databases
ProteinModelPortal	Q00218.
SMR	Q00218. Positions 49-493.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q00218. 3 interactions.
STRING	Q00218.
Proteomic databases
PRIDE	Q00218.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G33510.1; AT4G33510.1; AT4G33510.
GeneID	829489.
GenomeReviews	Gene locus AT4G33510 in contig CT486007_GR.
KEGG	ath:AT4G33510.
NMPDR	fig|3702.1.peg.21394.
Organism-specific databases
TAIR	At4g33510.
Phylogenomic databases
eggNOG	euNOG04328.
HOGENOM	HBG292577.
InParanoid	Q00218.
OMA	IYCSHEA.
PhylomeDB	Q00218.
ProtClustDB	PLN02291.
Gene expression databases
ArrayExpress	Q00218.
Genevestigator	Q00218.
GermOnline	AT4G33510. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002480. DAHP_synth_2. [Graphical view]
KO	K01626.
PANTHER	PTHR21337. DAHP_synth_2. 1 hit.
Pfam	PF01474. DAHP_synth_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01358. DAHP_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROG_ARATH

Accession

Primary (citable) accession number: Q00218
Secondary accession number(s): Q9SZC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	May 27, 2002
Last modified:	January 25, 2012
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program