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Q00216

- TIGA_ASPNG

UniProt

Q00216 - TIGA_ASPNG

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Protein

Protein disulfide-isomerase tigA

Gene
tigA
Organism
Aspergillus niger
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei49 – 491Nucleophile By similarity
Sitei50 – 501Contributes to redox potential value By similarity
Sitei51 – 511Contributes to redox potential value By similarity
Active sitei52 – 521Nucleophile By similarity
Sitei115 – 1151Lowers pKa of C-terminal Cys of first active site By similarity
Active sitei169 – 1691Nucleophile By similarity
Sitei170 – 1701Contributes to redox potential value By similarity
Sitei171 – 1711Contributes to redox potential value By similarity
Active sitei172 – 1721Nucleophile By similarity
Sitei236 – 2361Lowers pKa of C-terminal Cys of second active site By similarity

GO - Molecular functioni

  1. protein disulfide isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
  2. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase tigA (EC:5.3.4.1)
Gene namesi
Name:tigA
OrganismiAspergillus niger
Taxonomic identifieri5061 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

Endoplasmic reticulum lumen Reviewed prediction

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed predictionAdd
BLAST
Chaini20 – 359340Protein disulfide-isomerase tigAPRO_0000034245Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 52Redox-active By similarity
Disulfide bondi169 ↔ 172Redox-active By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

By stress, and tunicamycin.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ00216.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 129110Thioredoxin 1Add
BLAST
Domaini131 – 250120Thioredoxin 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi356 – 3594Prevents secretion from ER Reviewed prediction

Sequence similaritiesi

Contains 2 thioredoxin domains.

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiCOG0526.

Family and domain databases

Gene3Di1.20.1150.12. 1 hit.
3.40.30.10. 2 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR011679. ER_p29_C.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF07749. ERp29. 1 hit.
PF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF47933. SSF47933. 1 hit.
SSF52833. SSF52833. 2 hits.
TIGRFAMsiTIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00216-1 [UniParc]FASTAAdd to Basket

« Hide

MVRLSNLVSC LGLASAVTAA VVDLVPKNFD DVVLKSGKPA LVEFFAPWCG    50
HCKNLAPVYE ELGQAFAHAS DKVTVGKVDA DEHRDLGRKF GVQGFPTLKW 100
FDGKSDEPED YKGGRDLESL SSFISEKTGV KPRGPKKEPS KVEMLNDATF 150
KGAVGGDNDV LVAFTAPWCG HCKNLAPTWE ALANDFVLEP NVVIAKVDAD 200
AENGKATARE QGVSGYPTIK FFPKGSTESV PYEGARSEQA FIDFLNEKTG 250
THRTVGGGLD TKAGTIASLD ELIASTSAAD LAAAVKKAAT ELKDKYAQYY 300
VKVADKLSQN AEYAAKELAR LEKILAKGGS APEKVDDLIS RSNILRKFVG 350
EEKEAKDEL 359
Length:359
Mass (Da):38,592
Last modified:November 1, 1996 - v1
Checksum:i346B64197BA37608
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98748 Genomic DNA. Translation: CAA67299.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98748 Genomic DNA. Translation: CAA67299.1 .

3D structure databases

ProteinModelPortali Q00216.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0526.

Family and domain databases

Gene3Di 1.20.1150.12. 1 hit.
3.40.30.10. 2 hits.
InterProi IPR005788. Disulphide_isomerase.
IPR011679. ER_p29_C.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view ]
Pfami PF07749. ERp29. 1 hit.
PF00085. Thioredoxin. 2 hits.
[Graphical view ]
SUPFAMi SSF47933. SSF47933. 1 hit.
SSF52833. SSF52833. 2 hits.
TIGRFAMsi TIGR01126. pdi_dom. 2 hits.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and characterisation of a novel stress-inducible PDI-family gene from Aspergillus niger."
    Jeenes D.J., Pfaller R., Archer D.B.
    Gene 193:151-156(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

Entry informationi

Entry nameiTIGA_ASPNG
AccessioniPrimary (citable) accession number: Q00216
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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