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Protein

Alkaline protease 1

Gene

alp1

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Secreted alkaline protease that allows assimilation of proteinaceous substrates.1 Publication

Catalytic activityi

Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyze peptide amides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161Charge relay systemBy similarity1
Active sitei192Charge relay systemBy similarity1
Active sitei348Charge relay systemBy similarity1

GO - Molecular functioni

  • peptidase activity Source: UniProtKB
  • serine-type endopeptidase activity Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS08.053.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline protease 1 (EC:3.4.21.63)
Short name:
ALP
Alternative name(s):
Aspergillopeptidase B
Aspergillus proteinase B
Elastase
Elastinolytic serine proteinase
Oryzin
Gene namesi
Name:alp1
Synonyms:alk1, alp, prtA
ORF Names:AN5558
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome V
  • UP000005890 Componenti: Partially assembled WGS sequence

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000040699822 – 124By similarityAdd BLAST103
ChainiPRO_0000406999125 – 403Alkaline protease 1Add BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi252N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ00208.

Expressioni

Inductioni

Highly expressed on medium lacking a nitrogen, carbon or sulfur source.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00003523.

Structurei

3D structure databases

ProteinModelPortaliQ00208.
SMRiQ00208.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini142 – 364Peptidase S8Add BLAST223

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000199176.
InParanoidiQ00208.
KOiK18549.
OMAiHVDTLGH.
OrthoDBiEOG092C4A4B.

Family and domain databases

CDDicd04077. Peptidases_S8_PCSK9_Proteinase. 1 hit.
Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiView protein in InterPro
IPR034193. PCSK9_ProteinaseK-like.
IPR009020. Peptidase/Inhibitor_I9.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
PfamiView protein in Pfam
PF05922. Inhibitor_I9. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiView protein in PROSITE
PS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00208-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSFKRSLLL LGALLPAVFG APVEPRRAAE KVPGKYIVTF KSGLNVDQID
60 70 80 90 100
AHTSWASNVH KRNLERRGLA ERDQYSGIEK NYKINKFAAY SGSFDDATIE
110 120 130 140 150
EIRNSADVAH VEEDQIWYID ALTSQSGAPW GLGAISHKGE ASTTYVYDTS
160 170 180 190 200
AGEGTYAYVV DTGINADHEE FGGRASLAYN AVGGQHVDSV GHGTHVAGTI
210 220 230 240 250
GGETYGVSKK ANLLSVKVFQ GESSSTSIIL DGFNWAANDI VSKGRTGKSA
260 270 280 290 300
INMSLGGGYS YAFNQAVEDA YDEGVLSVVA AGNDNIDASD SSPASAPNAL
310 320 330 340 350
TVAASTKSNT RASFSNYGSV VDIFAPGQDI LSAWIGSTTA TNTISGTSMA
360 370 380 390 400
TPHVVGLSLY LIALEGLSSA SAVVSRIKEL ATQGVLSNVQ GSPNLLAYNG

ADE
Length:403
Mass (Da):42,209
Last modified:November 1, 1996 - v1
Checksum:i6971CD5B0E65E8C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31778 Genomic DNA. Translation: AAA67705.1.
AACD01000095 Genomic DNA. Translation: EAA62263.1.
BN001305 Genomic DNA. Translation: CBF81670.1.
RefSeqiXP_663162.1. XM_658070.1.

Genome annotation databases

EnsemblFungiiCADANIAT00003523; CADANIAP00003523; CADANIAG00003523.
EAA62263; EAA62263; AN5558.2.
GeneIDi2871850.
KEGGiani:AN5558.2.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiORYZ_EMENI
AccessioniPrimary (citable) accession number: Q00208
Secondary accession number(s): C8VG51, Q5B1M2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: November 1, 1996
Last modified: July 5, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families