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Protein

cGMP-gated cation channel alpha-1

Gene

CNGA1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cGMP which leads to an opening of the cation channel and thereby causing a depolarization of rod photoreceptors.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei544cGMPSequence analysis1
Binding sitei559cGMPSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi485 – 607cGMPSequence analysisAdd BLAST123

GO - Molecular functioni

  • cGMP binding Source: UniProtKB
  • intracellular cAMP activated cation channel activity Source: GO_Central
  • intracellular cGMP activated cation channel activity Source: UniProtKB
  • voltage-gated potassium channel activity Source: GO_Central

GO - Biological processi

  • cation transport Source: UniProtKB
  • regulation of membrane potential Source: GO_Central
  • response to stimulus Source: UniProtKB-KW
  • visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel

Keywords - Biological processi

Ion transport, Sensory transduction, Transport, Vision

Keywords - Ligandi

cGMP, cGMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-2485179. Activation of the phototransduction cascade.
R-BTA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-gated cation channel alpha-1
Alternative name(s):
Cyclic nucleotide-gated cation channel 1
Cyclic nucleotide-gated channel alpha-1
Short name:
CNG channel alpha-1
Short name:
CNG-1
Short name:
CNG1
Cyclic nucleotide-gated channel, photoreceptor
Rod photoreceptor cGMP-gated channel subunit alpha
Gene namesi
Name:CNGA1
Synonyms:CNCG, CNCG1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 6

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 162CytoplasmicCuratedAdd BLAST162
Transmembranei163 – 183Helical; Name=H1CuratedAdd BLAST21
Topological domaini184 – 196ExtracellularCuratedAdd BLAST13
Transmembranei197 – 215Helical; Name=H2CuratedAdd BLAST19
Topological domaini216 – 239CytoplasmicCuratedAdd BLAST24
Transmembranei240 – 259Helical; Name=H3CuratedAdd BLAST20
Topological domaini260 – 297ExtracellularCuratedAdd BLAST38
Transmembranei298 – 320Helical; Name=H4CuratedAdd BLAST23
Topological domaini321 – 372CytoplasmicCuratedAdd BLAST52
Transmembranei373 – 392Helical; Name=H5CuratedAdd BLAST20
Topological domaini393 – 476ExtracellularCuratedAdd BLAST84
Transmembranei477 – 497Helical; Name=H6CuratedAdd BLAST21
Topological domaini498 – 690CytoplasmicCuratedAdd BLAST193

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4907.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002193061 – 690cGMP-gated cation channel alpha-1Add BLAST690

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi423N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ00194.
PRIDEiQ00194.

PTM databases

iPTMnetiQ00194.

Expressioni

Tissue specificityi

Rod cells in the retina.

Gene expression databases

BgeeiENSBTAG00000002205.

Interactioni

Subunit structurei

Tetramer formed of three CNGA1 and one CNGB1 modulatory subunits.1 Publication

Protein-protein interaction databases

IntActiQ00194. 1 interactor.
MINTiMINT-6825023.
STRINGi9913.ENSBTAP00000002853.

Chemistry databases

BindingDBiQ00194.

Structurei

Secondary structure

1690
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi621 – 672Combined sources52

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SWFX-ray2.14A/B/C621-690[»]
ProteinModelPortaliQ00194.
SMRiQ00194.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00194.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili621 – 6641 PublicationAdd BLAST44

Domaini

The C-terminal coiled-coil domain mediates homotrimerization of CNGA subunits.

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0500. Eukaryota.
ENOG410YWWI. LUCA.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000007898.
HOVERGENiHBG000281.
InParanoidiQ00194.
KOiK04948.
OMAiYLEYWLI.
OrthoDBiEOG091G03EW.
TreeFamiTF319048.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR032406. CLZ_dom.
IPR032945. CNGA1.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF387. PTHR10217:SF387. 1 hit.
PfamiPF16526. CLZ. 1 hit.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00194-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVIINTWH SFVNIPNVVG PDVEKEITRM ENGACSSFSG DDDDSASMFE
60 70 80 90 100
ESETENPHAR DSFRSNTHGS GQPSQREQYL PGAIALFNVN NSSNKEQEPK
110 120 130 140 150
EKKKKKKEKK SKPDDKNENK KDPEKKKKKE KDKDKKKKEE KGKDKKEEEK
160 170 180 190 200
KEVVVIDPSG NTYYNWLFCI TLPVMYNWTM IIARACFDEL QSDYLEYWLA
210 220 230 240 250
FDYLSDVVYL LDMFVRTRTG YLEQGLLVKE ERKLIDKYKS TFQFKLDVLS
260 270 280 290 300
VIPTDLLYIK FGWNYPEIRL NRLLRISRMF EFFQRTETRT NYPNIFRISN
310 320 330 340 350
LVMYIIIIIH WNACVYFSIS KAIGFGNDTW VYPDVNDPDF GRLARKYVYS
360 370 380 390 400
LYWSTLTLTT IGETPPPVRD SEYFFVVADF LIGVLIFATI VGNIGSMISN
410 420 430 440 450
MNAARAEFQA RIDAIKQYMH FRNVSKDMEK RVIKWFDYLW TNKKTVDERE
460 470 480 490 500
VLKYLPDKLR AEIAINVHLD TLKKVRIFAD CEAGLLVELV LKLQPQVYSP
510 520 530 540 550
GDYICKKGDI GREMYIIKEG KLAVVADDGI TQFVVLSDGS YFGEISILNI
560 570 580 590 600
KGSKAGNRRT ANIKSIGYSD LFCLSKDDLM EALTEYPDAK GMLEEKGKQI
610 620 630 640 650
LMKDGLLDIN IANAGSDPKD LEEKVTRMES SVDLLQTRFA RILAEYESMQ
660 670 680 690
QKLKQRLTKV EKFLKPLIDT EFSAIEGSGT ESGPTDSTQD
Length:690
Mass (Da):79,602
Last modified:April 1, 1993 - v1
Checksum:iA01CFB6567424455
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51604 mRNA. Translation: CAA35947.1.
PIRiS07103.
RefSeqiNP_776703.1. NM_174278.2.
UniGeneiBt.53.

Genome annotation databases

EnsembliENSBTAT00000002853; ENSBTAP00000002853; ENSBTAG00000002205.
GeneIDi281700.
KEGGibta:281700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51604 mRNA. Translation: CAA35947.1.
PIRiS07103.
RefSeqiNP_776703.1. NM_174278.2.
UniGeneiBt.53.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SWFX-ray2.14A/B/C621-690[»]
ProteinModelPortaliQ00194.
SMRiQ00194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ00194. 1 interactor.
MINTiMINT-6825023.
STRINGi9913.ENSBTAP00000002853.

Chemistry databases

BindingDBiQ00194.
ChEMBLiCHEMBL4907.

PTM databases

iPTMnetiQ00194.

Proteomic databases

PaxDbiQ00194.
PRIDEiQ00194.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000002853; ENSBTAP00000002853; ENSBTAG00000002205.
GeneIDi281700.
KEGGibta:281700.

Organism-specific databases

CTDi1259.

Phylogenomic databases

eggNOGiKOG0500. Eukaryota.
ENOG410YWWI. LUCA.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000007898.
HOVERGENiHBG000281.
InParanoidiQ00194.
KOiK04948.
OMAiYLEYWLI.
OrthoDBiEOG091G03EW.
TreeFamiTF319048.

Enzyme and pathway databases

ReactomeiR-BTA-2485179. Activation of the phototransduction cascade.
R-BTA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

EvolutionaryTraceiQ00194.
PROiQ00194.

Gene expression databases

BgeeiENSBTAG00000002205.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR032406. CLZ_dom.
IPR032945. CNGA1.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF387. PTHR10217:SF387. 1 hit.
PfamiPF16526. CLZ. 1 hit.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNGA1_BOVIN
AccessioniPrimary (citable) accession number: Q00194
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.