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Q00141 (NCPR_ASPNG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADPH--cytochrome P450 reductase

Short name=CPR
Short name=P450R
EC=1.6.2.4
Gene names
Name:cprA
OrganismAspergillus niger
Taxonomic identifier5061 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length694 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis. Ref.1 Ref.2

Catalytic activity

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein. Ref.1 Ref.2

Cofactor

FAD. Ref.1 Ref.2

FMN. Ref.1 Ref.2

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Cell membrane; Single-pass membrane protein By similarity. Microsome By similarity UniProtKB P50126.

Induction

By benzoic acid (BA). Ref.1

Sequence similarities

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. UniProtKB P50126

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Sequence caution

The sequence CAA81550.1 differs from that shown. Reason: Frameshift at positions 34, 43, 392, 405, 421, 501 and 515.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 694694NADPH--cytochrome P450 reductase
PRO_0000167604

Regions

Transmembrane9 – 3123Helical; Potential
Domain66 – 220155Flavodoxin-like
Domain276 – 537262FAD-binding FR-type
Nucleotide binding72 – 765FMN By similarity
Nucleotide binding165 – 19632FMN By similarity UniProtKB P50126
Nucleotide binding316 – 32712FAD By similarity UniProtKB P50126
Nucleotide binding446 – 45712FAD By similarity
Nucleotide binding546 – 56419NADP By similarity
Nucleotide binding641 – 65717NADP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q00141 [UniParc].

Last modified February 8, 2011. Version 2.
Checksum: BA3E7867ACB1D810

FASTA69476,807
        10         20         30         40         50         60 
MAQLDTLDLV VLAVLLVGSV AYFTKGTYWA VAKDPYASTG PAMNGAAKAG KTRNIIEKME 

        70         80         90        100        110        120 
ETGKNCVIFY GSQTGTAEDY ASRLAKEGSQ RFGLKTMVAD LEEYDYENLD QFPEDKVAFF 

       130        140        150        160        170        180 
VLATYGEGEP TDNAVEFYQF FTGDDVAFES ASADEKPLSK LKYVAFGLGN NTYEHYNAMV 

       190        200        210        220        230        240 
RQVDAAFQKL GPQRIGSAGE GDDGAGTMEE DFLAWKEPMW AALSESMDLE EREAVYEPVF 

       250        260        270        280        290        300 
CVTENESLSP EDETVYLGEP TQSHLQGTPK GPYSAHNPFI APIAESRELF TVKDRNCLHM 

       310        320        330        340        350        360 
EISIAGSNLS YQTGDHIAVW PTNAGAEVDR FLQVFGLEGK RDSVINIKGI DVTAKVPIPT 

       370        380        390        400        410        420 
PTTYDAAVRY YMEVCAPVSR QFVATLAAFA PDEESKAEIV RLGSHKDYFH EKVTNQCFNM 

       430        440        450        460        470        480 
AQALQSITSK PFSAVPFSLL IEGITKLQPR YYSISSSSLV QKDKISITAV VESVRLPGAS 

       490        500        510        520        530        540 
HMVKGVTTNY LLALKQKQNG DPSPDPHGLT YSITGPRNKY DGIHVPVHVR HSNFKLPSDP 

       550        560        570        580        590        600 
SRPIIMVGPG TGVAPFRGFI QERAALAAKG EKVGPTVLFF GCRKSDEDFL YKDEWKTYQD 

       610        620        630        640        650        660 
QLGDNLKIIT AFSREGPQKV YVQHRLREHS ELVSDLLKQK ATFYVCGDAA NMAREVNLVL 

       670        680        690 
GQIIAAQRGL PAEKGEEMVK HMRRRGRYQE DVWS 

« Hide

References

[1]"Cloning and characterization of the NADPH cytochrome P450 oxidoreductase gene from the filamentous fungus Aspergillus niger."
van den Brink H.J.M., van Zeijl C.M.J., Brons J.F., van den Hondel C.A.M.J.J., van Gorcom R.F.M.
DNA Cell Biol. 14:719-729(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, INDUCTION.
Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.
[2]"Purification and characterization of benzoate-para-hydroxylase, a cytochrome P450 (CYP53A1), from Aspergillus niger."
Faber B.W., van Gorcom R.F.M., Duine J.A.
Arch. Biochem. Biophys. 394:245-254(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY.
Strain: T18.5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z26938 Genomic DNA. Translation: CAA81550.1. Frameshift.
PIRS38427.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ00141.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG0369.

Family and domain databases

Gene3D1.20.990.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFPIRSF000208. P450R. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNCPR_ASPNG
AccessionPrimary (citable) accession number: Q00141
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: February 8, 2011
Last modified: April 16, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families