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Q00141

- NCPR_ASPNG

UniProt

Q00141 - NCPR_ASPNG

Protein

NADPH--cytochrome P450 reductase

Gene

cprA

Organism
Aspergillus niger
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (08 Feb 2011)
      Previous versions | rss
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    Functioni

    This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.

    Catalytic activityi

    NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.2 Publications

    Cofactori

    FAD.2 Publications
    FMN.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 765FMNPROSITE-ProRule annotation
    Nucleotide bindingi165 – 19632FMNPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi316 – 32712FADBy similarityAdd
    BLAST
    Nucleotide bindingi446 – 45712FADBy similarityAdd
    BLAST
    Nucleotide bindingi546 – 56419NADPBy similarityAdd
    BLAST
    Nucleotide bindingi641 – 65717NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. electron carrier activity Source: UniProtKB
    2. FMN binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. NADPH-hemoprotein reductase activity Source: UniProtKB-EC
    5. oxidoreductase activity Source: UniProtKB

    GO - Biological processi

    1. oxidation-reduction process Source: UniProtKB
    2. sterol biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    FAD, Flavoprotein, FMN, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH--cytochrome P450 reductase (EC:1.6.2.4)
    Short name:
    CPR
    Short name:
    P450R
    Gene namesi
    Name:cprA
    OrganismiAspergillus nigerImported
    Taxonomic identifieri5061 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

    Subcellular locationi

    Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity. Cell membrane By similarity; Single-pass membrane protein By similarity. Microsome By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. intracellular membrane-bounded organelle Source: UniProtKB
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 694694NADPH--cytochrome P450 reductasePRO_0000167604Add
    BLAST

    Proteomic databases

    PRIDEiQ00141.

    Expressioni

    Inductioni

    By benzoic acid (BA).1 Publication

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 3123HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini66 – 220155Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini276 – 537262FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.By similarity
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0369.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000208. P450R. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00141-1 [UniParc]FASTAAdd to Basket

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    MAQLDTLDLV VLAVLLVGSV AYFTKGTYWA VAKDPYASTG PAMNGAAKAG    50
    KTRNIIEKME ETGKNCVIFY GSQTGTAEDY ASRLAKEGSQ RFGLKTMVAD 100
    LEEYDYENLD QFPEDKVAFF VLATYGEGEP TDNAVEFYQF FTGDDVAFES 150
    ASADEKPLSK LKYVAFGLGN NTYEHYNAMV RQVDAAFQKL GPQRIGSAGE 200
    GDDGAGTMEE DFLAWKEPMW AALSESMDLE EREAVYEPVF CVTENESLSP 250
    EDETVYLGEP TQSHLQGTPK GPYSAHNPFI APIAESRELF TVKDRNCLHM 300
    EISIAGSNLS YQTGDHIAVW PTNAGAEVDR FLQVFGLEGK RDSVINIKGI 350
    DVTAKVPIPT PTTYDAAVRY YMEVCAPVSR QFVATLAAFA PDEESKAEIV 400
    RLGSHKDYFH EKVTNQCFNM AQALQSITSK PFSAVPFSLL IEGITKLQPR 450
    YYSISSSSLV QKDKISITAV VESVRLPGAS HMVKGVTTNY LLALKQKQNG 500
    DPSPDPHGLT YSITGPRNKY DGIHVPVHVR HSNFKLPSDP SRPIIMVGPG 550
    TGVAPFRGFI QERAALAAKG EKVGPTVLFF GCRKSDEDFL YKDEWKTYQD 600
    QLGDNLKIIT AFSREGPQKV YVQHRLREHS ELVSDLLKQK ATFYVCGDAA 650
    NMAREVNLVL GQIIAAQRGL PAEKGEEMVK HMRRRGRYQE DVWS 694
    Length:694
    Mass (Da):76,807
    Last modified:February 8, 2011 - v2
    Checksum:iBA3E7867ACB1D810
    GO

    Sequence cautioni

    The sequence CAA81550.1 differs from that shown. Reason: Frameshift at positions 34, 43, 392, 405, 421, 501 and 515.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26938 Genomic DNA. Translation: CAA81550.1. Frameshift.
    PIRiS38427.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26938 Genomic DNA. Translation: CAA81550.1 . Frameshift.
    PIRi S38427.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q00141.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG0369.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000208. P450R. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the NADPH cytochrome P450 oxidoreductase gene from the filamentous fungus Aspergillus niger."
      van den Brink H.J.M., van Zeijl C.M.J., Brons J.F., van den Hondel C.A.M.J.J., van Gorcom R.F.M.
      DNA Cell Biol. 14:719-729(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, INDUCTION.
      Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.
    2. "Purification and characterization of benzoate-para-hydroxylase, a cytochrome P450 (CYP53A1), from Aspergillus niger."
      Faber B.W., van Gorcom R.F.M., Duine J.A.
      Arch. Biochem. Biophys. 394:245-254(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY.
      Strain: T18.5.

    Entry informationi

    Entry nameiNCPR_ASPNG
    AccessioniPrimary (citable) accession number: Q00141
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: February 8, 2011
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3