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Protein

NADPH--cytochrome P450 reductase

Gene

cprA

Organism
Aspergillus niger
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation2 Publications

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei203 – 2031FMNUniRule annotation
Binding sitei295 – 2951NADPUniRule annotation
Binding sitei551 – 5511NADPUniRule annotation
Binding sitei655 – 6551NADPUniRule annotation
Binding sitei693 – 6931FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 776FMNUniRule annotation
Nucleotide bindingi123 – 1264FMNUniRule annotation
Nucleotide bindingi168 – 17710FMNUniRule annotation
Nucleotide bindingi450 – 4534FADUniRule annotation
Nucleotide bindingi468 – 4703FADUniRule annotation
Nucleotide bindingi485 – 4884FADUniRule annotation
Nucleotide bindingi613 – 6142NADPUniRule annotation
Nucleotide bindingi619 – 6235NADPUniRule annotation

GO - Molecular functioni

  • electron carrier activity Source: UniProtKB
  • FMN binding Source: InterPro
  • iron ion binding Source: InterPro
  • NADPH-hemoprotein reductase activity Source: UniProtKB-EC
  • oxidoreductase activity Source: UniProtKB

GO - Biological processi

  • oxidation-reduction process Source: UniProtKB
  • sterol biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:cprAUniRule annotation
OrganismiAspergillus nigerImported
Taxonomic identifieri5061 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

  • Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Mitochondrion outer membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Cell membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88LumenalUniRule annotation
Transmembranei9 – 3123HelicalUniRule annotationAdd
BLAST
Topological domaini32 – 694663CytoplasmicUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 694694NADPH--cytochrome P450 reductasePRO_0000167604Add
BLAST

Proteomic databases

PaxDbiQ00141.
PRIDEiQ00141.

Expressioni

Inductioni

By benzoic acid (BA).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00006979.

Structurei

3D structure databases

ProteinModelPortaliQ00141.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 220155Flavodoxin-likeUniRule annotationAdd
BLAST
Domaini276 – 537262FAD-binding FR-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00141-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLDTLDLV VLAVLLVGSV AYFTKGTYWA VAKDPYASTG PAMNGAAKAG
60 70 80 90 100
KTRNIIEKME ETGKNCVIFY GSQTGTAEDY ASRLAKEGSQ RFGLKTMVAD
110 120 130 140 150
LEEYDYENLD QFPEDKVAFF VLATYGEGEP TDNAVEFYQF FTGDDVAFES
160 170 180 190 200
ASADEKPLSK LKYVAFGLGN NTYEHYNAMV RQVDAAFQKL GPQRIGSAGE
210 220 230 240 250
GDDGAGTMEE DFLAWKEPMW AALSESMDLE EREAVYEPVF CVTENESLSP
260 270 280 290 300
EDETVYLGEP TQSHLQGTPK GPYSAHNPFI APIAESRELF TVKDRNCLHM
310 320 330 340 350
EISIAGSNLS YQTGDHIAVW PTNAGAEVDR FLQVFGLEGK RDSVINIKGI
360 370 380 390 400
DVTAKVPIPT PTTYDAAVRY YMEVCAPVSR QFVATLAAFA PDEESKAEIV
410 420 430 440 450
RLGSHKDYFH EKVTNQCFNM AQALQSITSK PFSAVPFSLL IEGITKLQPR
460 470 480 490 500
YYSISSSSLV QKDKISITAV VESVRLPGAS HMVKGVTTNY LLALKQKQNG
510 520 530 540 550
DPSPDPHGLT YSITGPRNKY DGIHVPVHVR HSNFKLPSDP SRPIIMVGPG
560 570 580 590 600
TGVAPFRGFI QERAALAAKG EKVGPTVLFF GCRKSDEDFL YKDEWKTYQD
610 620 630 640 650
QLGDNLKIIT AFSREGPQKV YVQHRLREHS ELVSDLLKQK ATFYVCGDAA
660 670 680 690
NMAREVNLVL GQIIAAQRGL PAEKGEEMVK HMRRRGRYQE DVWS
Length:694
Mass (Da):76,807
Last modified:February 8, 2011 - v2
Checksum:iBA3E7867ACB1D810
GO

Sequence cautioni

The sequence CAA81550.1 differs from that shown. Reason: Frameshift at positions 34, 43, 392, 405, 421, 501 and 515. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26938 Genomic DNA. Translation: CAA81550.1. Frameshift.
PIRiS38427.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26938 Genomic DNA. Translation: CAA81550.1. Frameshift.
PIRiS38427.

3D structure databases

ProteinModelPortaliQ00141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5061.CADANGAP00006979.

Proteomic databases

PaxDbiQ00141.
PRIDEiQ00141.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG1158. Eukaryota.
COG0369. LUCA.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of the NADPH cytochrome P450 oxidoreductase gene from the filamentous fungus Aspergillus niger."
    van den Brink H.J.M., van Zeijl C.M.J., Brons J.F., van den Hondel C.A.M.J.J., van Gorcom R.F.M.
    DNA Cell Biol. 14:719-729(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, INDUCTION.
    Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.
  2. "Purification and characterization of benzoate-para-hydroxylase, a cytochrome P450 (CYP53A1), from Aspergillus niger."
    Faber B.W., van Gorcom R.F.M., Duine J.A.
    Arch. Biochem. Biophys. 394:245-254(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.
    Strain: T18.5.

Entry informationi

Entry nameiNCPR_ASPNG
AccessioniPrimary (citable) accession number: Q00141
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: February 8, 2011
Last modified: January 20, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.