Reviewed, UniProtKB/Swiss-Prot Q00078 (KPC1_ASPNG)

Names and origin

Protein names

Recommended name:
    Protein kinase C-like
    EC=2.7.11.13

Gene names

Name:

pkcA

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	1096 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain. Contains 2 REM (Hr1) repeats.

Ontologies

Keywords
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase C activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1096	1096	Protein kinase C-like		PRO_0000055742
Regions
	Repeat	5 – 77	73	REM 1
	Repeat	160 – 235	76	REM 2
	Domain	771 – 1030	260	Protein kinase
	Domain	1031 – 1096	66	AGC-kinase C-terminal
	Zinc finger	459 – 507	49	Phorbol-ester/DAG-type 1
	Zinc finger	527 – 577	51	Phorbol-ester/DAG-type 2
	Nucleotide binding	777 – 785	9	ATP By similarity
Sites
	Active site	896	1	Proton acceptor By similarity
	Binding site	800	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q00078-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 859B2DB3B30D5BD8	FASTA	1,096	122,235
	10 20 30 40 50 60 MDGDDLIASV YRKIEREKAL ITAASNMRQS TDNPLVQQRV DANIRDGRKN IAYLEEKMRE 70 80 90 100 110 120 LQLRRMEHEG GGSPTDKRLP PNPEAGPVPP QKDYGPGYGG NEEYGDASGA YPHGGAGSMP 130 140 150 160 170 180 SGAPYADPRP FAPVPKARPN YTKLDLIKYD TQYLGPKIQL MLSQLEFKLS VEKQYKAGIE 190 200 210 220 230 240 KMVRLYQDEG DRKSRADAEG RRIESNQKIQ LLKQALKRYE DLHVDIESTD TPDDESLSTP 250 260 270 280 290 300 NLRKPLTGLL TLRIHAVKDV DHAASSRFSR GPETFIVLKV EDTIKARTKA TRIDRWQDET 310 320 330 340 350 360 FNIDIDKANE IELTVYDKSG DRPTPIGMLW VRISDIAEEM RRKKIESEFN ASGWVSADKM 370 380 390 400 410 420 EHGGAPGRPD TAGGAGSAHP SGPGGPGAAP SQGYGDGSAG APAGNQVMID SWFALEPVGR 430 440 450 460 470 480 IYLSMSFAKQ LKDRRPFDIG LNRQGAVRQK KEEVHEKQGH KFVTQQFYNI MRCALCGDFL 490 500 510 520 530 540 KYAAGMQCAD CKYTCHKKCY PKVVTKCISK ANYETDPDEE KINHRIPHRF EGFSNISANW 550 560 570 580 590 600 CCHCGYLLPF GRKNAKRCTE CGLTCHSHCT HLVPDFCGMS MEAANQILET LIRTKNHNKT 610 620 630 640 650 660 ASVSSGLSGR TLRPSGTPQG PQDSAAMAYP QKPVESPYGA PQRQPSAEAV SAATNSYMPP 670 680 690 700 710 720 QSPTAAARQQ MPPRTSSSAA VAAATVATGM PSPQQMPAEQ NRPMQPQLPT YDPKAYEPYA 730 740 750 760 770 780 NAPQGMPPQA IQHAAPAGYG MPAQQPAMQM VSQQPPEAPQ QPKVRIGLDH FNFLAVLGKG 790 800 810 820 830 840 NFGKVMLAET KSTKRLYAIK VLKKEFIIEN DEVESTKSEK RVFMIANKER HPFLLNLHAC 850 860 870 880 890 900 FQTETRVYFV MEYISGGDLM LHIQRGQFGL KRAQFYAAEV LLALKYFHEN GVIYRDLKLD 910 920 930 940 950 960 NIMLTLDGHI KVADYGLCKE NMWYGATTST FCGTPEFMAP EILLDKRYGR AVDWWAFGVL 970 980 990 1000 1010 1020 IYQMLLQQSP FRGEDEDEIY DAILADEPLY PIHMPRDSVS ILQKLLTREP ELRLGSGPTD 1030 1040 1050 1060 1070 1080 AQEVMSHAFF RNINWDDIYH KRVPPPFLPQ ISSPTDTSNF DQEFTSVTPV LTPVQSVLSQ 1090 AMQEEFRGFS YTADFA

References

[1]

"Cloning and characterisation of genes (pkc1 and pkcA) encoding protein kinase C homologues from Trichoderma reesei and Aspergillus niger." Morawetz R., Lendenfeld T., Mischak H., Muehlbauer M., Gruber F., Goodnight J., de Graaff L.H., Visser J., Mushinski J.F., Kubicek C.P. Mol. Gen. Genet. 250:17-28(1996) [PubMed: 8569684] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U10549 Genomic DNA. Translation: AAA97433.1.
PIR	S61917.
3D structure databases
HSSP	HSSP built from PDB template 1GZK based on UniProtKB P31751.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.13. 277.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000008. C2_Ca-dep. IPR000861. HR1-like_rho-bd. IPR011072. HR1_rho-bd. IPR015745. PKC. IPR017892. Pkinase_C. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22985:SF86. PKC. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00168. C2. 1 hit. PF02185. HR1. 2 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00239. C2. 1 hit. SM00742. Hr1. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPC1_ASPNG

Accession

Primary (citable) accession number: Q00078

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	July 28, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)