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Q00024

- PPO1_AGABI

UniProt

Q00024 - PPO1_AGABI

Protein

Polyphenol oxidase 1

Gene

PPO1

Organism
Agaricus bisporus (White button mushroom)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Copper-containing oxidase that catalyzes both the o-hydroxylation of monophenols and the subsequent oxidation of the resulting o-diphenols into reactive o-quinones, which evolve spontaneously to produce intermediates, which associate in dark brown pigments. Involved in the initial step of melanin synthesis. Melanins constitute a mechanism of defense and resistance to stress such as UV radiations, free radicals, gamma rays, dehydratation and extreme temperatures, and contribute to the fungal cell-wall resistance against hydrolytic enzymes in avoiding cellular lysis. Fungal pigments are also involved in the formation and stability of spores By similarity.By similarity

    Catalytic activityi

    2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
    L-tyrosine + O2 = dopaquinone + H2O.

    Cofactori

    Binds 2 copper ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi57 – 571Copper 1; via tele nitrogenBy similarity
    Metal bindingi85 – 851Copper 1; via tele nitrogenBy similarity
    Metal bindingi94 – 941Copper 1; via tele nitrogenBy similarity
    Metal bindingi257 – 2571Copper 2; via tele nitrogenBy similarity
    Metal bindingi261 – 2611Copper 2; via tele nitrogenBy similarity
    Binding sitei261 – 2611SubstrateBy similarity
    Metal bindingi286 – 2861Copper 2; via tele nitrogenBy similarity
    Sitei382 – 3832CleavageCurated

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. monophenol monooxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. melanin biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Melanin biosynthesis

    Keywords - Ligandi

    Copper, Metal-binding

    Enzyme and pathway databases

    SABIO-RKQ00024.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyphenol oxidase 1 (EC:1.14.18.1)
    Short name:
    PPO1
    Short name:
    Phenolase 1
    Alternative name(s):
    Cresolase 1
    Tyrosinase 1
    Gene namesi
    Name:PPO1
    OrganismiAgaricus bisporus (White button mushroom)
    Taxonomic identifieri5341 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAgaricaceaeAgaricus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 568568Polyphenol oxidase 1PRO_0000186740Add
    BLAST
    Propeptidei383 – 565183Removed in mature formCuratedPRO_0000416862Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki83 ↔ 852'-(S-cysteinyl)-histidine (Cys-His)By similarity

    Post-translational modificationi

    The C-ter is probably cleaved after Gly-382 since the mature active protein is smaller than the protein encoded by the gene.By similarity

    Keywords - PTMi

    Thioether bond

    Interactioni

    Subunit structurei

    Heterotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ00024.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tyrosinase family.Curated

    Family and domain databases

    Gene3Di1.10.1280.10. 1 hit.
    InterProiIPR016216. Monophenol_mOase_fun.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view]
    PfamiPF00264. Tyrosinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000340. MPO_fungal. 1 hit.
    PRINTSiPR00092. TYROSINASE.
    SUPFAMiSSF48056. SSF48056. 1 hit.
    PROSITEiPS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q00024-1 [UniParc]FASTAAdd to Basket

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    MSHLLVSPLG GGVQPRLEIN NFVKNDRQFS LYVQALDRMY ATPQNETASY    50
    FQVAGVHGYP LIPFDDAVGP TEFSPFDQWT GYCTHGSTLF PTWHRPYVLI 100
    LEQILSGHAQ QIADTYTVNK SEWKKAATEF RHPYWDWASN SVPPPEVISL 150
    PKVTITTPNG QKTSVANPLM RYTFNSVNDG GFYGPYNQWD TTLRQPDSTG 200
    VNAKDNVNRL KSVLKNAQAS LTRATYDMFN RVTTWPHFSS HTPASGGSTS 250
    NSIEAIHDNI HVLVGGNGHM SDPSVAPFDP IFFLHHANVD RLIALWSAIR 300
    YDVWTSPGDA QFGTYTLRYK QSVDESTDLA PWWKTQNEYW KSNELRSTES 350
    LGYTYPEFVG LDMYNKDAVN KTISRKVAQL YGPQRGGQRS LVEDLSNSHA 400
    RRSQRPAKRS RLGQLLKGLF SDWSAQIKFN RHEVGQSFSV CLFLGNVPED 450
    PREWLVSPNL VGARHAFVRS VKTDHVAEEI GFIPINQWIA EHTGLPSFAV 500
    DLVKPLLAQG LQWRVLLADG TPAELDSLEV TILEVPSELT DDEPNPRSRP 550
    PRYHKDITHG KRGGCREA 568
    Length:568
    Mass (Da):63,898
    Last modified:November 1, 1996 - v1
    Checksum:iF5CBADB73533685A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85113 mRNA. Translation: CAA59432.1.
    PIRiS53017.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85113 mRNA. Translation: CAA59432.1 .
    PIRi S53017.

    3D structure databases

    ProteinModelPortali Q00024.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK Q00024.

    Family and domain databases

    Gene3Di 1.10.1280.10. 1 hit.
    InterProi IPR016216. Monophenol_mOase_fun.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view ]
    Pfami PF00264. Tyrosinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000340. MPO_fungal. 1 hit.
    PRINTSi PR00092. TYROSINASE.
    SUPFAMi SSF48056. SSF48056. 1 hit.
    PROSITEi PS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression and characterisation of two tyrosinase cDNAs from Agaricus bisporus."
      Wichers H., Recourt K., Hendriks M., Ebbelaar C.E.M., Biancone G., Hoeberichts F., Mooibroek H., Soler-Rivas C.
      Appl. Microbiol. Biotechnol. 61:336-341(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Horst U1.

    Entry informationi

    Entry nameiPPO1_AGABI
    AccessioniPrimary (citable) accession number: Q00024
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3