Q00023 (CEL1_AGABI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cellulose-growth-specific protein

Gene names

Name:

cel1

Organism

Agaricus bisporus (Common mushroom)

Taxonomic identifier

5341 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Agaricaceae › Agaricus

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Probable glycosyl hydrolase active on cellulose.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 61 family. Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulose binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 320	291	Cellulose-growth-specific protein		PRO_0000008031
Regions
	Domain	284 – 320	37	CBM1
	Region	30 – 261	232	Catalytic Potential
	Region	262 – 285	24	Linker Potential
Amino acid modifications
	Glycosylation	163	1	N-linked (GlcNAc...) Potential
	Disulfide bond	292 ↔ 309		By similarity
	Disulfide bond	303 ↔ 319		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q00023 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 60E2C8080895CA2B	FASTA	320	33,754
	10 20 30 40 50 60 MRLPSRQQVL KMLATFSLAL GLFAAKVQAH GGVIGYSWDG TWYEGWHPYN TPVGQTSIER 70 80 90 100 110 120 PWATFDPIMD ATASTVGCNN DGNPGPNQLT ATVAAGTAIT AYWNQVWPHP YGPMTTYLGK 130 140 150 160 170 180 CPGSSCDGVN TNSLKWFKID EAGLLSGTVG KGVWGSGKMI DQNNSWTTTI PSTVPSGAYM 190 200 210 220 230 240 IRFETIALHS LPAQIYPECA QLTITGGGNR APTSSELVSF PGGYSNSDPG LTVNLYTQEA 250 260 270 280 290 300 MTDTTYIVPG PPLYGSGGNG GSPTTTPHTT TPITTSPPPT STPGTIPQYG QCGGIGWTGG 310 320 TGCVAPYQCK VINDYYSQCL

References

[1]	"Isolation and characterization of a cellulose-growth-specific gene from Agaricus bisporus." Raguz S., Yaguee E., Wood D.A., Thurston C.F. Gene 119:183-190(1992) [PubMed: 1398098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D649.
[2]	"CEL1: a novel cellulose binding protein secreted by Agaricus bisporus during growth on crystalline cellulose." Armesilla A.L., Thurston C.F., Yaguee E. FEMS Microbiol. Lett. 116:293-299(1994) [PubMed: 8181702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D649.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M86356 Genomic DNA. Translation: AAA53434.1.
PIR	JC1311.
3D structure databases
ProteinModelPortal	Q00023.
SMR	Q00023. Positions 285-320.
ModBase	Search...
Protein family/group databases
CAZy	CBM1. Carbohydrate-Binding Module Family 1. GH61. Glycoside Hydrolase Family 61.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000254. Cellulose-bd_dom_fun. IPR005103. Glyco_hydro_61. [Graphical view]
Pfam	PF00734. CBM_1. 1 hit. PF03443. Glyco_hydro_61. 1 hit. [Graphical view]
ProDom	PD001821. CBD_fun. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00236. fCBD. 1 hit. [Graphical view]
SUPFAM	SSF57180. CBD_fun. 1 hit.
PROSITE	PS00562. CBM1_1. 1 hit. PS51164. CBM1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CEL1_AGABI

Accession

Primary (citable) accession number: Q00023

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 11, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program