Reviewed, UniProtKB/Swiss-Prot Q00016 (IFR_CICAR)

Names and origin

Protein names

Recommended name:
    Isoflavone reductase
      Short name=IFR
    EC=1.3.1.45
Alternative name(s):
    2'-hydroxyisoflavone reductase
    NADPH:isoflavone oxidoreductase

Gene names

Name:

IFR

Organism

Cicer arietinum (Chickpea) (Garbanzo)

Taxonomic identifier

3827 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Cicereae › Cicer

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduces achiral isoflavones to chiral isoflavanones during the biosynthesis of chiral pterocarpan phytoalexins.
Catalytic activity	Vestitone + NADP+ = 2'-hydroxyformononetin + NADPH.
Pathway	Phytoalexin biosynthesis; pterocarpan phytoalexin biosynthesis.
Sequence similarities	Belongs to the NmrA-type oxidoreductase family. Isoflavone reductase subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of nitrogen utilization Inferred from electronic annotation. Source: InterPro
Molecular function	2'-hydroxyisoflavone reductase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro transcription repressor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Isoflavone reductase		PRO_0000204545
Regions
	Nucleotide binding	6 – 37	32	NADP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q00016-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 437A1AD6843E0773	FASTA	318	35,408
	10 20 30 40 50 60 MASQNRILVL GPTGAIGRHV VWASIKAGNP TYALIRKTPG DINKPSLVAA ANPESKEELL 70 80 90 100 110 120 QSFKAAGVIL LEGDMNDHEA LVKAIKQVDT VICTFGRLLI LDQVKIIKAI KEAGNVKRFF 130 140 150 160 170 180 PSEFGLDVDR HDAVDPVRPV FDEKASIRRV VEAEGVPYTY LCCHAFTGYF LRNLAQFDAT 190 200 210 220 230 240 EPPRDKVIIL GDGNVKGAYV TEADVGTYTI RAANDPRTLN KAVHIRLPHN YLTSNEVVSL 250 260 270 280 290 300 WEKKIGKTLE KSYISEEKVL KDINVSTFPH NYLLALYHSQ QIKGDAVYEI DPAKDAEAYD 310 LYPDVKYTTA DEYLDQFV

References

[1]	"Pterocarpan phytoalexin biosynthesis in elicitor-challenged chickpea (Cicer arietinum L.) cell cultures. Purification, characterization and cDNA cloning of NADPH:isoflavone oxidoreductase." Tiemann K., Inze D., van Montagu M., Barz W. Eur. J. Biochem. 200:751-757(1991) [PubMed: 1915347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. ILC 3279.
[2]	"Isolation of NADPH:isoflavone oxidoreductase, a new enzyme of Pterocarpan phytoalexin biosynthesis in cell suspension cultures of Cicer arietinum." Tiemann K., Hinderer W., Barz W. FEBS Lett. 213:324-328(1987) Cited for: CHARACTERIZATION. Strain: cv. ILC 3279.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X60755 mRNA. Translation: CAA43167.1.
PIR	S17830.
3D structure databases
SMR	Q00016. Positions 3-318.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.1.45. 475.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR008030. NmrA. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF05368. NmrA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IFR_CICAR

Accession

Primary (citable) accession number: Q00016

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)