Reviewed, UniProtKB/Swiss-Prot P14714 (PHYC_ARATH)

Names and origin

Protein names

Recommended name:
    Phytochrome C

Gene names

Name:	PHYC
Ordered Locus Names:	At5g35840
ORF Names:	MIK22.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1111 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region. Photoconversion of Pr to Pfr induces an array of morphogenic responses, whereas reconversion of Pfr to Pr cancels the induction of those responses. Pfr controls the expression of a number of nuclear genes including those encoding the small subunit of ribulose-bisphosphate carboxylase, chlorophyll A/B binding protein, protochlorophyllide reductase, rRNA, etc. It also controls the expression of its own gene(s) in a negative feedback fashion.
Subunit structure	Homodimer.
Post-translational modification	Contains one covalently linked phytochromobilin chromophore By similarity.
Sequence similarities	Belongs to the phytochrome family. Contains 1 GAF domain. Contains 1 histidine kinase domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 2 PAS (PER-ARNT-SIM) domains.

Ontologies

Keywords
Biological process	Sensory transduction Transcription Transcription regulation
Domain	Repeat
Ligand	Chromophore
Molecular function	Photoreceptor protein Receptor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro protein-chromophore linkage Inferred from electronic annotation. Source: InterPro protein-tetrapyrrole linkage Inferred from electronic annotation. Source: InterPro red, far-red light phototransduction Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro G-protein coupled photoreceptor activity Inferred from electronic annotation. Source: InterPro protein homodimerization activity Inferred from electronic annotation. Source: InterPro two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PHYB	P14713	1	EBI-624366,EBI-300727

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1111	1111	Phytochrome C		PRO_0000171964
Regions
	Domain	213 – 393	181	GAF
	Domain	604 – 674	71	PAS 1
	Domain	737 – 808	72	PAS 2
	Domain	889 – 1111	223	Histidine kinase
Sites
	Binding site	318	1	Phytochromobilin chromophore (covalent; via 1 link) By similarity
Experimental info
	Sequence conflict	9	1	C → Y in CAA83549. Ref.2
	Sequence conflict	137	1	E → Q in CAA83549. Ref.2
	Sequence conflict	230	1	G → S in CAA83549. Ref.2
	Sequence conflict	446	1	N → K in CAA83549. Ref.2
	Sequence conflict	505	1	S → T in CAA83549. Ref.2
	Sequence conflict	726	1	T → K in CAA83549. Ref.2
	Sequence conflict	735	1	K → Q in CAA83549. Ref.2
	Sequence conflict	754	1	I → M in CAA83549. Ref.2
	Sequence conflict	822	1	E → K in CAA83549. Ref.2
	Sequence conflict	1017 – 1018	2	LR → WK in CAA83549. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P14714-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 58FE4645501135CA	FASTA	1,111	123,722
	10 20 30 40 50 60 MSSNTSRSCS TRSRQNSRVS SQVLVDAKLH GNFEESERLF DYSASINLNM PSSSCEIPSS 70 80 90 100 110 120 AVSTYLQKIQ RGMLIQPFGC LIVVDEKNLK VIAFSENTQE MLGLIPHTVP SMEQREALTI 130 140 150 160 170 180 GTDVKSLFLS PGCSALEKAV DFGEISILNP ITLHCRSSSK PFYAILHRIE EGLVIDLEPV 190 200 210 220 230 240 SPDEVPVTAA GALRSYKLAA KSISRLQALP SGNMLLLCDA LVKEVSELTG YDRVMVYKFH 250 260 270 280 290 300 EDGHGEVIAE CCREDMEPYL GLHYSATDIP QASRFLFMRN KVRMICDCSA VPVKVVQDKS 310 320 330 340 350 360 LSQPISLSGS TLRAPHGCHA QYMSNMGSVA SLVMSVTING SDSDEMNRDL QTGRHLWGLV 370 380 390 400 410 420 VCHHASPRFV PFPLRYACEF LTQVFGVQIN KEAESAVLLK EKRILQTQSV LCDMLFRNAP 430 440 450 460 470 480 IGIVTQSPNI MDLVKCDGAA LYYRDNLWSL GVTPTETQIR DLIDWVLKSH GGNTGFTTES 490 500 510 520 530 540 LMESGYPDAS VLGESICGMA AVYISEKDFL FWFRSSTAKQ IKWGGARHDP NDRDGKRMHP 550 560 570 580 590 600 RSSFKAFMEI VRWKSVPWDD MEMDAINSLQ LIIKGSLQEE HSKTVVDVPL VDNRVQKVDE 610 620 630 640 650 660 LCVIVNEMVR LIDTAAVPIF AVDASGVING WNSKAAEVTG LAVEQAIGKP VSDLVEDDSV 670 680 690 700 710 720 ETVKNMLALA LEGSEERGAE IRIRAFGPKR KSSPVELVVN TCCSRDMTNN VLGVCFIGQD 730 740 750 760 770 780 VTGQKTLTEN YSRVKGDYAR IMWSPSTLIP PIFITNENGV CSEWNNAMQK LSGIKREEVV 790 800 810 820 830 840 NKILLGEVFT TDDYGCCLKD HDTLTKLRIG FNAVISGQKN IEKLLFGFYH RDGSFIEALL 850 860 870 880 890 900 SANKRTDIEG KVTGVLCFLQ VPSPELQYAL QVQQISEHAI ACALNKLAYL RHEVKDPEKA 910 920 930 940 950 960 ISFLQDLLHS SGLSEDQKRL LRTSVLCREQ LAKVISDSDI EGIEEGYVEL DCSEFGLQES 970 980 990 1000 1010 1020 LEAVVKQVME LSIERKVQIS CDYPQEVSSM RLYGDNLRLQ QILSETLLSS IRFTPALRGL 1030 1040 1050 1060 1070 1080 CVSFKVIARI EAIGKRMKRV ELEFRIIHPA PGLPEDLVRE MFQPLRKGTS REGLGLHITQ 1090 1100 1110 KLVKLMERGT LRYLRESEMS AFVILTEFPL I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family." Sharrock R.A., Quail P.H. Genes Dev. 3:1745-1757(1989) [PubMed: 2606345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"The PHYC gene of Arabidopsis. Absence of the third intron found in PHYA and PHYB." Cowl J.S., Hartley N., Xie D., Whitelam G.C., Murphy G., Harberd N.P. Plant Physiol. 106:813-814(1994) [PubMed: 7991704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17343 mRNA. Translation: CAA35223.1. Z32538 Genomic DNA. Translation: CAA83549.1. AB005236 Genomic DNA. Translation: BAB09925.1.
PIR	FKMUC. C33473.
RefSeq	NP_198433.1.
UniGene	At.97
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P14714.
Genome annotation databases
GeneID	833570.
GenomeReviews	Gene locus AT5G35840 in contig BA000015_GR.
KEGG	ath:AT5G35840.
NMPDR	fig|3702.1.peg.25291.
Organism-specific databases
TAIR	At5g35840.
Gene expression databases
ArrayExpress	P14714.
GermOnline	AT5G35840. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR003018. GAF. IPR001610. PAC. IPR000014. PAS. IPR013654. PAS_2. IPR013767. PAS_fold. IPR016132. Phyto_chromo_attachment. IPR013516. Phyto_chromo_BS. IPR001294. Phytochrome. IPR012129. Phytochrome_A-E. IPR013515. Phytochrome_Cr. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF01590. GAF. 1 hit. PF02518. HATPase_c. 1 hit. PF00989. PAS. 2 hits. PF08446. PAS_2. 1 hit. PF00360. Phytochrome. 1 hit. [Graphical view]
PIRSF	PIRSF000084. Phytochrome. 1 hit.
PRINTS	PR01033. PHYTOCHROME.
SMART	SM00065. GAF. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00086. PAC. 1 hit. SM00091. PAS. 2 hits. [Graphical view]
TIGRFAMs	TIGR00229. sensory_box. 1 hit.
PROSITE	PS50109. HIS_KIN. 1 hit. PS50112. PAS. 2 hits. PS00245. PHYTOCHROME_1. 1 hit. PS50046. PHYTOCHROME_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHYC_ARATH

Accession

Primary (citable) accession number: P14714

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	November 25, 2008
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)