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Reviewed, UniProtKB/Swiss-Prot Q05793 (PGBM_MOUSE)

Last modified July 22, 2008. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Basement membrane-specific heparan sulfate proteoglycan core protein
      Short name=HSPG
      Short name=Perlecan
      Short name=PLC
Gene names
Name: Hspg2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length3707 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

This protein is an integral component of basement membranes. It is responsible for the fixed negative electrostatic charge and is involved in the charge-selective ultrafiltration properties. It serves as an attachment substrate for cells.

Subunit structure

Purified perlecan has a strong tendency to aggregate in dimers or stellate structures. It interacts with other basement membrane components such as laminin, prolargin and collagen type IV. Interacts with COL13A1, FGFBP1 and VWA1.

Subcellular location

Secreted › extracellular space › extracellular matrix › basement membrane.

Tissue specificity

Found in the basement membranes.

Post-translational modification

Contains three heparan sulfate chains as well as N-linked and O-linked oligosaccharides.

Sequence similarities

Contains 1 EGF-like domain.

Contains 15 Ig-like C2-type (immunoglobulin-like) domains.

Contains 11 laminin EGF-like domains.

Contains 3 laminin G-like domains.

Contains 3 laminin IV type A domains.

Contains 4 LDL-receptor class A domains.

Contains 1 SEA domain.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

Nid1P104931EBI-1032089,EBI-1032117

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 37073686Basement membrane-specific heparan sulfate proteoglycan core protein

Regions

Domain80 – 194115SEA
Domain195 – 23440LDL-receptor class A 1
Domain281 – 31939LDL-receptor class A 2
Domain320 – 35940LDL-receptor class A 3
Domain360 – 40344LDL-receptor class A 4
Domain404 – 504101Ig-like C2-type 1
Domain521 – 53010Laminin EGF-like 1; first part
Domain538 – 730193Laminin IV type A 1
Domain731 – 76333Laminin EGF-like 1; second part
Domain764 – 81350Laminin EGF-like 2
Domain814 – 87158Laminin EGF-like 3
Domain879 – 92345Laminin EGF-like 4; truncated
Domain924 – 93310Laminin EGF-like 5; first part
Domain941 – 1125185Laminin IV type A 2
Domain1126 – 115833Laminin EGF-like 5; second part
Domain1159 – 120850Laminin EGF-like 6
Domain1209 – 126557Laminin EGF-like 7
Domain1275 – 132450Laminin EGF-like 8
Domain1325 – 133410Laminin EGF-like 9; first part
Domain1344 – 1529186Laminin IV type A 3
Domain1530 – 156233Laminin EGF-like 9; second part
Domain1563 – 161250Laminin EGF-like 10
Domain1613 – 167058Laminin EGF-like 11
Domain1677 – 177195Ig-like C2-type 2
Domain1772 – 186594Ig-like C2-type 3
Domain1866 – 195489Ig-like C2-type 4
Domain1955 – 204995Ig-like C2-type 5
Domain2050 – 214899Ig-like C2-type 6
Domain2149 – 224496Ig-like C2-type 7
Domain2245 – 234399Ig-like C2-type 8
Domain2344 – 243693Ig-like C2-type 9
Domain2437 – 253296Ig-like C2-type 10
Domain2533 – 261987Ig-like C2-type 11
Domain2620 – 2720101Ig-like C2-type 12
Domain2721 – 280989Ig-like C2-type 13
Domain2810 – 289586Ig-like C2-type 14
Domain2896 – 298085Ig-like C2-type 15
Domain2984 – 3162179Laminin G-like 1
Domain3163 – 324179EGF-like
Domain3245 – 3425181Laminin G-like 2
Domain3518 – 3705188Laminin G-like 3
Region3615 – 36173Mediates motor neuron attachment Potential

Amino acid modifications

Glycosylation651O-linked (Xyl...) (heparan sulfate) Potential
Glycosylation711O-linked (Xyl...) (heparan sulfate) Potential
Glycosylation761O-linked (Xyl...) (heparan sulfate) Potential
Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation3581N-linked (GlcNAc...) Potential
Glycosylation5541N-linked (GlcNAc...) Potential
Glycosylation12561N-linked (GlcNAc...) Potential
Glycosylation18911N-linked (GlcNAc...) Potential
Glycosylation23361N-linked (GlcNAc...) Potential
Glycosylation23941N-linked (GlcNAc...) Potential
Glycosylation24271N-linked (GlcNAc...) Potential
Glycosylation26001N-linked (GlcNAc...) Potential
Glycosylation30981N-linked (GlcNAc...) Potential
Glycosylation31541N-linked (GlcNAc...) Potential
Glycosylation33851N-linked (GlcNAc...) Potential
Disulfide bond199 ↔ 212 By similarity
Disulfide bond206 ↔ 225 By similarity
Disulfide bond219 ↔ 234 By similarity
Disulfide bond285 ↔ 297 By similarity
Disulfide bond292 ↔ 310 By similarity
Disulfide bond304 ↔ 319 By similarity
Disulfide bond325 ↔ 337 By similarity
Disulfide bond332 ↔ 350 By similarity
Disulfide bond344 ↔ 359 By similarity
Disulfide bond368 ↔ 381 By similarity
Disulfide bond375 ↔ 394 By similarity
Disulfide bond388 ↔ 403 By similarity
Disulfide bond428 ↔ 479 By similarity
Disulfide bond764 ↔ 773 By similarity
Disulfide bond766 ↔ 780 By similarity
Disulfide bond783 ↔ 792 By similarity
Disulfide bond795 ↔ 811 By similarity
Disulfide bond814 ↔ 829 By similarity
Disulfide bond816 ↔ 839 By similarity
Disulfide bond842 ↔ 851 By similarity
Disulfide bond854 ↔ 869 By similarity
Disulfide bond879 ↔ 892 By similarity
Disulfide bond894 ↔ 903 By similarity
Disulfide bond906 ↔ 921 By similarity
Disulfide bond1159 ↔ 1168 By similarity
Disulfide bond1161 ↔ 1175 By similarity
Disulfide bond1178 ↔ 1187 By similarity
Disulfide bond1190 ↔ 1206 By similarity
Disulfide bond1209 ↔ 1224 By similarity
Disulfide bond1211 ↔ 1234 By similarity
Disulfide bond1237 ↔ 1246 By similarity
Disulfide bond1249 ↔ 1263 By similarity
Disulfide bond