Reviewed, UniProtKB/Swiss-Prot Q8S9M1 (PAP13_ARATH)

Names and origin

Protein names

Recommended name:
    Probable plastid-lipid-associated protein 13, chloroplastic
Alternative name(s):
    Fibrillin-13
      Short name=AtPGL30

Gene names

Name:	PAP13
Ordered Locus Names:	At2g42130
ORF Names:	T24P15.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Plastid › chloroplast › plastoglobule.
Sequence similarities	Belongs to the PAP/fibrillin family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	plastoglobule Ref.6 Inferred from direct assay. Source: TAIR thylakoid Inferred from direct assay. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8S9M1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Notes: No experimental confirmation available.

Isoform 2 (identifier: Q8S9M1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     253-270: GSFERFFMISYLDEEILI → KLLYPFFIHLTIFRSNLH
     271-299: Missing.

Notes: No experimental confirmation available.

Isoform 3 (identifier: Q8S9M1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Notes: No experimental confirmation available.

Isoform 4 (identifier: Q8S9M1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.
     78-78: A → AEQSSVCLELSNYFCPEAIYVQ

Notes: No experimental confirmation available.

Isoform 5 (identifier: Q8S9M1-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.
     148-155: RFPSTLAN → SVLIIFSS
     156-299: Missing.

Notes: No experimental confirmation available.

Isoform 6 (identifier: Q8S9M1-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-158: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 48	48	Chloroplast Potential
	Chain	49 – 299	251	Probable plastid-lipid-associated protein 13, chloroplastic		PRO_0000286536
Natural variations
	Alternative sequence	1 – 158	158	Missing in isoform 6.		VSP_025073
	Alternative sequence	1 – 49	49	Missing in isoform 3, isoform 4 and isoform 5.		VSP_025074
	Alternative sequence	78	1	A → AEQSSVCLELSNYFCPEAIY VQ in isoform 4.		VSP_025075
	Alternative sequence	148 – 155	8	RFPSTLAN → SVLIIFSS in isoform 5.		VSP_025076
	Alternative sequence	156 – 299	144	Missing in isoform 5.		VSP_025077
	Alternative sequence	253 – 270	18	GSFER…EEILI → KLLYPFFIHLTIFRSNLH in isoform 2.		VSP_025078
	Alternative sequence	271 – 299	29	Missing in isoform 2.		VSP_025079

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 15, 2007. Version 2. Checksum: E73AC3466B2F1A3D	FASTA	299	32,986
	10 20 30 40 50 60 MALIHGSVPG TSAVRLVFST SASPSRFCLN VPVVKQGWKN SCRRRVLRAM VQETVQGSPL 70 80 90 100 110 120 VYAREMERLS AKESLLLALK DAGGFEALVT GKTTNMQRID VNERITSLER LNPTPRPTTS 130 140 150 160 170 180 PCFEGRWNFE WFGSGSPGLL AARVIFERFP STLANLSRME ILIKDANAKA TANIKLLNSI 190 200 210 220 230 240 ESKIILSSKL TVEGPLRLKE EYVEGMLETP TVIEEAVPEQ LKSALGQAAT TLQQLPALIK 250 260 270 280 290 DTLASGLRIP LSGSFERFFM ISYLDEEILI VRDTEGVPEV LTRIETPSST VVETIEYDS
	Isoform 2 [UniParc]. Checksum: 122B1A81847CEC11		270	29,832
	10 20 30 40 50 60 MALIHGSVPG TSAVRLVFST SASPSRFCLN VPVVKQGWKN SCRRRVLRAM VQETVQGSPL 70 80 90 100 110 120 VYAREMERLS AKESLLLALK DAGGFEALVT GKTTNMQRID VNERITSLER LNPTPRPTTS 130 140 150 160 170 180 PCFEGRWNFE WFGSGSPGLL AARVIFERFP STLANLSRME ILIKDANAKA TANIKLLNSI 190 200 210 220 230 240 ESKIILSSKL TVEGPLRLKE EYVEGMLETP TVIEEAVPEQ LKSALGQAAT TLQQLPALIK 250 260 270 DTLASGLRIP LSKLLYPFFI HLTIFRSNLH
	Isoform 3 [UniParc]. Checksum: 1AE511E39D911EB4		250	27,663
	10 20 30 40 50 60 MVQETVQGSP LVYAREMERL SAKESLLLAL KDAGGFEALV TGKTTNMQRI DVNERITSLE 70 80 90 100 110 120 RLNPTPRPTT SPCFEGRWNF EWFGSGSPGL LAARVIFERF PSTLANLSRM EILIKDANAK 130 140 150 160 170 180 ATANIKLLNS IESKIILSSK LTVEGPLRLK EEYVEGMLET PTVIEEAVPE QLKSALGQAA 190 200 210 220 230 240 TTLQQLPALI KDTLASGLRI PLSGSFERFF MISYLDEEIL IVRDTEGVPE VLTRIETPSS 250 TVVETIEYDS
	Isoform 4 [UniParc]. Checksum: 437A60202D26BC32		271	30,067
	10 20 30 40 50 60 MVQETVQGSP LVYAREMERL SAKESLLLAE QSSVCLELSN YFCPEAIYVQ LKDAGGFEAL 70 80 90 100 110 120 VTGKTTNMQR IDVNERITSL ERLNPTPRPT TSPCFEGRWN FEWFGSGSPG LLAARVIFER 130 140 150 160 170 180 FPSTLANLSR MEILIKDANA KATANIKLLN SIESKIILSS KLTVEGPLRL KEEYVEGMLE 190 200 210 220 230 240 TPTVIEEAVP EQLKSALGQA ATTLQQLPAL IKDTLASGLR IPLSGSFERF FMISYLDEEI 250 260 270 LIVRDTEGVP EVLTRIETPS STVVETIEYD S
	Isoform 5 [UniParc]. Checksum: 73A2F924FDD088A0		106	11,763
	10 20 30 40 50 60 MVQETVQGSP LVYAREMERL SAKESLLLAL KDAGGFEALV TGKTTNMQRI DVNERITSLE 70 80 90 100 RLNPTPRPTT SPCFEGRWNF EWFGSGSPGL LAARVIFESV LIIFSS
	Isoform 6 [UniParc]. Checksum: ACD1A609E8115064		141	15,521
	10 20 30 40 50 60 MEILIKDANA KATANIKLLN SIESKIILSS KLTVEGPLRL KEEYVEGMLE TPTVIEEAVP 70 80 90 100 110 120 EQLKSALGQA ATTLQQLPAL IKDTLASGLR IPLSGSFERF FMISYLDEEI LIVRDTEGVP 130 140 EVLTRIETPS STVVETIEYD S

References

[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 6). Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[4]	"Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2 reveals numerous transcript variants." Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L., Ayele M., Haas B.J., Wu H.C., Town C.D. Plant Physiol. 139:1323-1337(2005) [PubMed: 16244158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3; 4 AND 5). Strain: cv. Columbia.
[5]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: cv. Columbia.
[6]	"Tocopherol cyclase (VTE1) localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles." Vidi P.-A., Kanwischer M., Baginsky S., Austin J.R., Csucs G., Doermann P., Kessler F., Brehelin C. J. Biol. Chem. 281:11225-11234(2006) [PubMed: 16414959] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[7]	"Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes." Ytterberg A.J., Peltier J.-B., van Wijk K.J. Plant Physiol. 140:984-997(2006) [PubMed: 16461379] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC002561 Genomic DNA. Translation: AAB88638.2. AK117864 mRNA. Translation: BAC42505.1. AY074656 mRNA. Translation: AAL69472.1. AY219066 mRNA. Translation: AAO37153.1. AY219067 mRNA. Translation: AAO37154.1. AY219068 mRNA. Translation: AAO37155.1. AY954828 mRNA. Translation: AAX55154.1.
PIR	T00923.
RefSeq	NP_973667.2.
UniGene	At.37008
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	818813.
GenomeReviews	Gene locus AT2G42130 in contig CT485783_GR.
NMPDR	fig|3702.1.peg.11358.
Organism-specific databases
GeneFarm	2866. 270.
TAIR	At2g42130.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

PAP13_ARATH

Accession

Primary (citable) accession number: Q8S9M1
Secondary accession number(s): O48521 , Q84X37, Q84X38, Q84X39, Q8GY49

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	November 25, 2008
This is version 23 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)