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Protein

Vitamin B12 transport ATP-binding protein BacA

Gene

bacA

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ABC transporter involved in uptake of vitamin B12 and related corrinoids. Transmembrane domains (TMD) form a pore in the membrane and the ATP-binding domain (NBD) is responsible for energy generation. Contributes to maintenance of chronic infections.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi460 – 467ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • response to host immune response Source: MTBBASE

Keywordsi

Biological processTransport
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12 transport ATP-binding protein BacA
Gene namesi
Name:bacA
Ordered Locus Names:Rv1819c
ORF Names:MTCY1A11.24c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv1819c.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei20 – 40HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei68 – 88HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei121 – 141HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei213 – 233HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei249 – 269HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei332 – 352HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

  • extracellular region Source: MTBBASE
  • integral component of plasma membrane Source: MTBBASE
  • plasma membrane Source: MTBBASE

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption results in bleomycin resistance and alters the outcome of a chronic infection within the host, but does not alter sensitivity to other membrane-disrupting agents and resistance to clinically important antituberculosis drugs. Deletion eliminates the ability of the bacterium to transport vitamin B12 and related corrinoids.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000932651 – 639Vitamin B12 transport ATP-binding protein BacAAdd BLAST639

Proteomic databases

PaxDbiP9WQI9.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi83332.Rv1819c.

Structurei

3D structure databases

ProteinModelPortaliP9WQI9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini67 – 393ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST327
Domaini427 – 639ABC transporterPROSITE-ProRule annotationAdd BLAST213

Sequence similaritiesi

Belongs to the ABC transporter superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CQ0. Bacteria.
COG4178. LUCA.
KOiK02471.
OMAiTIIHMNR.
PhylomeDBiP9WQI9.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF06472. ABC_membrane_2. 1 hit.
PF00005. ABC_tran. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 3 hits.
PROSITEiView protein in PROSITE
PS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P9WQI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPKLFKPSI DWSRAFPDSV YWVGKAWTIS AICVLAILVL LRYLTPWGRQ
60 70 80 90 100
FWRITRAYFV GPNSVRVWLM LGVLLLSVVL AVRLNVLFSY QGNDMYTALQ
110 120 130 140 150
KAFEGIASGD GTVKRSGVRG FWMSIGVFSV MAVLHVTRVM ADIYLTQRFI
160 170 180 190 200
IAWRVWLTHH LTQDWLDGRA YYRDLFIDET IDNPDQRIQQ DVDIFTAGAG
210 220 230 240 250
GTPNAPSNGT ASTLLFGAVQ SIISVISFTA ILWNLSGTLN IFGVSIPRAM
260 270 280 290 300
FWTVLVYVFV ATVISFIIGR PLIWLSFRNE KLNAAFRYAL VRLRDAAEAV
310 320 330 340 350
GFYRGERVEG TQLQRRFTPV IDNYRRYVRR SIAFNGWNLS VSQTIVPLPW
360 370 380 390 400
VIQAPRLFAG QIDFGDVGQT ATSFGNIHDS LSFFRNNYDA FASFRAAIIR
410 420 430 440 450
LHGLVDANEK GRALPAVLTR PSDDESVELN DIEVRTPAGD RLIDPLDVRL
460 470 480 490 500
DRGGSLVITG RSGAGKTTLL RSLAELWPYA SGTLHRPGGE NETMFLSQLP
510 520 530 540 550
YVPLGTLRDV VCYPNSAAAI PDATLRDTLT KVALAPLCDR LDEERDWAKV
560 570 580 590 600
LSPGEQQRVA FARILLTKPK AVFLDESTSA LDTGLEFALY QLLRSELPDC
610 620 630
IVISVSHRPA LERLHENQLE LLGGGQWRLA PVEAAPAEV
Length:639
Mass (Da):71,317
Last modified:April 16, 2014 - v1
Checksum:iDE85BBEEF42759A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44585.1.
PIRiD70720.
RefSeqiNP_216335.1. NC_000962.3.
WP_003900416.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP44585; CCP44585; Rv1819c.
GeneIDi885539.
KEGGimtu:Rv1819c.
mtv:RVBD_1819c.
PATRICifig|83332.111.peg.2024.

Similar proteinsi

Entry informationi

Entry nameiBACA_MYCTU
AccessioniPrimary (citable) accession number: P9WQI9
Secondary accession number(s): L0TAH3, Q50614
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: June 7, 2017
This is version 19 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families