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Protein

Phthiocerol synthesis polyketide synthase type I PpsE

Gene

ppsE

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the elongation of either C22-24 fatty acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol derivatives.1 Publication

Miscellaneous

Was identified as a high-confidence drug target.

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO2 + [acyl-carrier-protein].PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei184For beta-ketoacyl synthase activityPROSITE-ProRule annotation1
Active sitei641For malonyltransferase activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1286 – 1331NADPBy similarityAdd BLAST46

GO - Molecular functioni

GO - Biological processi

  • Actinobacterium-type cell wall biogenesis Source: UniProtKB
  • DIM/DIP cell wall layer assembly Source: MTBBASE
  • fatty acid biosynthetic process Source: MTBBASE
  • lipid biosynthetic process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase, Transferase
Biological processFatty acid metabolism, Lipid metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Phthiocerol synthesis polyketide synthase type I PpsE
Alternative name(s):
Beta-ketoacyl-acyl-carrier-protein synthase I (EC:2.3.1.41)
Gene namesi
Name:ppsE
Ordered Locus Names:Rv2935
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2935.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • plasma membrane Source: MTBBASE
  • polyketide synthase complex Source: UniProtKB

Pathology & Biotechi

Disruption phenotypei

Cells lacking this genes infect fewer macrophages derived from monocytes (MDMs) than did the WT.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00004069522 – 1488Phthiocerol synthesis polyketide synthase type I PpsEAdd BLAST1487

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei965O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1

Keywords - PTMi

Acetylation, Phosphopantetheine, Phosphoprotein

Proteomic databases

PaxDbiP9WQE1.

PTM databases

iPTMnetiP9WQE1.

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv2935.

Structurei

3D structure databases

ProteinModelPortaliP9WQE1.
SMRiP9WQE1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini930 – 1004CarrierPROSITE-ProRule annotationAdd BLAST75

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni6 – 389Beta-ketoacyl synthaseBy similarityAdd BLAST384
Regioni551 – 868AcyltransferaseBy similarityAdd BLAST318

Phylogenomic databases

eggNOGiENOG4107EEY. Bacteria.
COG3321. LUCA.
KOiK12444.
OMAiWSQRCAG.
PhylomeDBiP9WQE1.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.30.70.250. 1 hit.
3.40.47.10. 2 hits.
InterProiView protein in InterPro
IPR036736. ACP-like_sf.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR001242. Condensatn.
IPR032821. KAsynt_C_assoc.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR020806. PKS_PP-bd.
IPR009081. PP-bd_ACP.
IPR016039. Thiolase-like.
PfamiView protein in Pfam
PF00698. Acyl_transf_1. 1 hit.
PF00668. Condensation. 1 hit.
PF16197. KAsynt_C_assoc. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF00550. PP-binding. 1 hit.
SMARTiView protein in SMART
SM00827. PKS_AT. 1 hit.
SM00825. PKS_KS. 1 hit.
SM00823. PKS_PP. 1 hit.
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 3 hits.
SSF55048. SSF55048. 1 hit.
PROSITEiView protein in PROSITE
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS50075. CARRIER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WQE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIPENAIAV VGMAGRFPGA KDVSAFWSNL RRGKESIVTL SEQELRDAGV
60 70 80 90 100
SDKTLADPAY VRRAPLLDGI DEFDAGFFGF PPLAAQVLDP QHRLFLQCAW
110 120 130 140 150
HALEDAGADP ARFDGSIGVY GTSSPSGYLL HNLLSHRDPN AVLAEGLNFD
160 170 180 190 200
QFSLFLQNDK DFLATRISHA FNLRGPSIAV QTACSSSLVA VHLACLSLLS
210 220 230 240 250
GECDMALAGG SSLCIPHRVG YFTSPGSMVS AVGHCRPFDV RADGTVFGSG
260 270 280 290 300
VGLVVLKPLA AAIDAGDRIH AVIRGSAINN DGSAKMGYAA PNPAAQADVI
310 320 330 340 350
AEAHAVSGID SSTVSYVECH GTGTPLGDPI EIQGLRAAFE VSQTSRSAPC
360 370 380 390 400
VLGSVKSNIG HLEVAAGIAG LIKTILCLKN KALPATLHYT SPNPELRLDQ
410 420 430 440 450
SPFVVQSKYG PWECDGVRRA GVSSFGVGGT NAHVVLEEAP AEASEVSAHA
460 470 480 490 500
EPAGPQVILL SAQTAAALGE SRTALAAALE TQDGPRLSDV AYTLARRRKH
510 520 530 540 550
NVTMAAVVHD REHAATVLRA AEHDNVFVGE AAHDGEHGDR ADAAPTSDRV
560 570 580 590 600
VFLFPGQGAQ HVGMAKGLYD TEPVFAQHFD TCAAGFRDET GIDLHAEVFD
610 620 630 640 650
GTATDLERID RSQPALFTVE YALAKLVDTF GVRAGAYIGY STGEYIAATL
660 670 680 690 700
AGVFDLQTAI KTVSLRARLM HESPPGAMVA VALGPDDVTQ YLPPEVELSA
710 720 730 740 750
VNDPGNCVVA GPKDQIRALR QRLTEAGIPV RRVRATHAFH TSAMDPMLGQ
760 770 780 790 800
FQEFLSRQQL RPPRTPLLSN LTGSWMSDQQ VVDPASWTRQ ISSPIRFADE
810 820 830 840 850
LDVVLAAPSR ILVEVGPGGS LTGSAMRHPK WSTTHRTVRL MRHPLQDVDD
860 870 880 890 900
RDTFLRALGE LWSAGVEVDW TPRRPAVPHL VSLPGYPFAR QRHWVEPNHT
910 920 930 940 950
VWAQAPGANN GSPAGTADGS TAATVDAARN GESQTEVTLQ RIWSQCLGVS
960 970 980 990 1000
SVDRNANFFD LGGDSLMAIS IAMAAANEGL TITPQDLYEY PTLASLTAAV
1010 1020 1030 1040 1050
DASFASSGLA KPPEAQANPA VPPNVTYFLD RGLRDTGRCR VPLILRLDPK
1060 1070 1080 1090 1100
IGLPDIRAVL TAVVNHHDAL RLHLVGNDGI WEQHIAAPAE FTGLSNRSVP
1110 1120 1130 1140 1150
NGVAAGSPEE RAAVLGILAE LLEDQTDPNA PLAAVHIAAA HGGPHYLCLA
1160 1170 1180 1190 1200
IHAMVTDDSS RQILATDIVT AFGQRLAGEE ITLEPVSTGW REWSLRCAAL
1210 1220 1230 1240 1250
ATHPAALDTR SYWIENSTKA TLWLADALPN AHTAHPPRAD ELTKLSSTLS
1260 1270 1280 1290 1300
VEQTSELDDG RRRFRRSIQT ILLAALGRTI AQTVGEGVVA VELEGEGRSV
1310 1320 1330 1340 1350
LRPDVDLRRT VGWFTTYYPV PLACATGLGA LAQLDAVHNT LKSVPHYGIG
1360 1370 1380 1390 1400
YGLLRYVYAP TGRVLGAQRT PDIHFRYAGV IPELPSGDAP VQFDSDMTLP
1410 1420 1430 1440 1450
VREPIPGMGH AIELRVYRFG GSLHLDWWYD TRRIPAATAE ALERTFPLAL
1460 1470 1480
SALIQEAIAA EHTEHDDSEI VGEPEAGALV DLSSMDAG
Length:1,488
Mass (Da):158,745
Last modified:April 16, 2014 - v1
Checksum:iC6418D88A27BB701
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP45738.1.
PIRiC70984.
RefSeqiNP_217451.1. NC_000962.3.
WP_003900602.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP45738; CCP45738; Rv2935.
GeneIDi888210.
KEGGimtu:Rv2935.

Similar proteinsi

Entry informationi

Entry nameiPPSE_MYCTU
AccessioniPrimary (citable) accession number: P9WQE1
Secondary accession number(s): L0TDU0, P96204, Q7D6E9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: October 25, 2017
This is version 27 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways