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Protein

3-phosphoshikimate 1-carboxyvinyltransferase

Gene

aroA

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.UniRule annotation1 Publication

Catalytic activityi

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.UniRule annotation1 Publication

Pathwayi: chorismate biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotationCurated
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (LH57_11870), Phospho-2-dehydro-3-deoxyheptonate aldolase AroG (aroG)
  2. 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ)
  4. no protein annotated in this organism
  5. Shikimate kinase (aroK), Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC), Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28Shikimate-3-phosphateUniRule annotation1 Publication1
Binding sitei124PhosphoenolpyruvateUniRule annotation1 Publication1
Binding sitei196Shikimate-3-phosphateUniRule annotation1 Publication1
Active sitei311Proton acceptorUniRule annotation1 Publication1
Binding sitei336Shikimate-3-phosphate1 Publication1
Binding sitei340Shikimate-3-phosphate1 Publication1
Active sitei341Proton donorUniRule annotation1
Binding sitei344PhosphoenolpyruvateUniRule annotation1 Publication1
Binding sitei385PhosphoenolpyruvateUniRule annotation1 Publication1
Binding sitei410PhosphoenolpyruvateUniRule annotation1 Publication1

GO - Molecular functioni

  • 3-phosphoshikimate 1-carboxyvinyltransferase activity Source: MTBBASE

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-7501-MONOMER.
ReactomeiR-MTU-964903. Chorismate via Shikimate Pathway.
UniPathwayiUPA00053; UER00089.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase1 PublicationUniRule annotation (EC:2.5.1.19UniRule annotation1 Publication)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase1 PublicationUniRule annotation
Short name:
EPSP synthase1 PublicationUniRule annotation
Short name:
EPSPS1 PublicationUniRule annotation
Gene namesi
Name:aroA1 PublicationUniRule annotation
Ordered Locus Names:Rv3227
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv3227.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: Reactome
  • plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000882731 – 4503-phosphoshikimate 1-carboxyvinyltransferaseAdd BLAST450

Proteomic databases

PaxDbiP9WPY5.

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi83332.Rv3227.

Structurei

Secondary structure

1450
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 17Combined sources4
Helixi23 – 38Combined sources16
Beta strandi44 – 47Combined sources4
Helixi53 – 64Combined sources12
Beta strandi68 – 70Combined sources3
Beta strandi72 – 74Combined sources3
Beta strandi76 – 80Combined sources5
Beta strandi89 – 91Combined sources3
Helixi96 – 106Combined sources11
Beta strandi109 – 116Combined sources8
Helixi119 – 123Combined sources5
Helixi127 – 135Combined sources9
Beta strandi139 – 141Combined sources3
Beta strandi143 – 150Combined sources8
Beta strandi158 – 162Combined sources5
Turni164 – 167Combined sources4
Helixi169 – 177Combined sources9
Helixi178 – 180Combined sources3
Beta strandi181 – 183Combined sources3
Beta strandi185 – 189Combined sources5
Helixi197 – 209Combined sources13
Beta strandi221 – 224Combined sources4
Beta strandi233 – 235Combined sources3
Helixi240 – 253Combined sources14
Beta strandi256 – 259Combined sources4
Helixi270 – 279Combined sources10
Beta strandi283 – 287Combined sources5
Beta strandi290 – 294Combined sources5
Beta strandi303 – 305Combined sources3
Helixi310 – 312Combined sources3
Helixi313 – 321Combined sources9
Beta strandi328 – 333Combined sources6
Helixi335 – 339Combined sources5
Beta strandi340 – 342Combined sources3
Helixi344 – 354Combined sources11
Beta strandi358 – 362Combined sources5
Beta strandi365 – 369Combined sources5
Beta strandi376 – 378Combined sources3
Helixi384 – 394Combined sources11
Beta strandi400 – 403Combined sources4
Helixi405 – 410Combined sources6
Helixi415 – 423Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BJBX-ray1.80A1-450[»]
2O0BX-ray1.15A1-450[»]
2O0DX-ray1.60A1-450[»]
2O0EX-ray1.81A1-450[»]
2O0XX-ray1.96A1-450[»]
2O0ZX-ray2.00A1-450[»]
2O15X-ray1.95A1-450[»]
ProteinModelPortaliP9WPY5.
SMRiP9WPY5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 24Shikimate-3-phosphate bindingUniRule annotation1 Publication2
Regioni94 – 97Phosphoenolpyruvate bindingUniRule annotation1 Publication4
Regioni167 – 169Shikimate-3-phosphate bindingUniRule annotation1 Publication3

Sequence similaritiesi

Belongs to the EPSP synthase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105CMY. Bacteria.
COG0128. LUCA.
KOiK00800.
OMAiKSYPDFW.
PhylomeDBiP9WPY5.

Family and domain databases

CDDicd01556. EPSP_synthase. 1 hit.
Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth. 1 hit.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WPY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTWPAPTAP TPVRATVTVP GSKSQTNRAL VLAALAAAQG RGASTISGAL
60 70 80 90 100
RSRDTELMLD ALQTLGLRVD GVGSELTVSG RIEPGPGARV DCGLAGTVLR
110 120 130 140 150
FVPPLAALGS VPVTFDGDQQ ARGRPIAPLL DALRELGVAV DGTGLPFRVR
160 170 180 190 200
GNGSLAGGTV AIDASASSQF VSGLLLSAAS FTDGLTVQHT GSSLPSAPHI
210 220 230 240 250
AMTAAMLRQA GVDIDDSTPN RWQVRPGPVA ARRWDIEPDL TNAVAFLSAA
260 270 280 290 300
VVSGGTVRIT GWPRVSVQPA DHILAILRQL NAVVIHADSS LEVRGPTGYD
310 320 330 340 350
GFDVDLRAVG ELTPSVAALA ALASPGSVSR LSGIAHLRGH ETDRLAALST
360 370 380 390 400
EINRLGGTCR ETPDGLVITA TPLRPGIWRA YADHRMAMAG AIIGLRVAGV
410 420 430 440 450
EVDDIAATTK TLPEFPRLWA EMVGPGQGWG YPQPRSGQRA RRATGQGSGG
Length:450
Mass (Da):46,426
Last modified:April 16, 2014 - v1
Checksum:i27BB86F9412A07D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52269 Genomic DNA. Translation: CAA36510.1.
M62708 Genomic DNA. Translation: AAA25356.1.
AL123456 Genomic DNA. Translation: CCP46046.1.
PIRiE70590.
RefSeqiNP_217744.1. NC_000962.3.
WP_003416914.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP46046; CCP46046; Rv3227.
GeneIDi888753.
KEGGimtu:Rv3227.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52269 Genomic DNA. Translation: CAA36510.1.
M62708 Genomic DNA. Translation: AAA25356.1.
AL123456 Genomic DNA. Translation: CCP46046.1.
PIRiE70590.
RefSeqiNP_217744.1. NC_000962.3.
WP_003416914.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BJBX-ray1.80A1-450[»]
2O0BX-ray1.15A1-450[»]
2O0DX-ray1.60A1-450[»]
2O0EX-ray1.81A1-450[»]
2O0XX-ray1.96A1-450[»]
2O0ZX-ray2.00A1-450[»]
2O15X-ray1.95A1-450[»]
ProteinModelPortaliP9WPY5.
SMRiP9WPY5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv3227.

Proteomic databases

PaxDbiP9WPY5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP46046; CCP46046; Rv3227.
GeneIDi888753.
KEGGimtu:Rv3227.

Organism-specific databases

TubercuListiRv3227.

Phylogenomic databases

eggNOGiENOG4105CMY. Bacteria.
COG0128. LUCA.
KOiK00800.
OMAiKSYPDFW.
PhylomeDBiP9WPY5.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00089.
BioCyciMTBH37RV:G185E-7501-MONOMER.
ReactomeiR-MTU-964903. Chorismate via Shikimate Pathway.

Family and domain databases

CDDicd01556. EPSP_synthase. 1 hit.
Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth. 1 hit.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROA_MYCTU
AccessioniPrimary (citable) accession number: P9WPY5
Secondary accession number(s): L0TDI9, P22487
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 30, 2016
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.