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Protein

Chorismate synthase

Gene

aroC

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.UniRule annotation

Miscellaneous

Was identified as a high-confidence drug target.

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.UniRule annotation

Cofactori

FMNH2UniRule annotation1 PublicationNote: Reduced FMN (FMNH(2)).UniRule annotation1 Publication

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (LH57_11870), Phospho-2-dehydro-3-deoxyheptonate aldolase AroG (aroG)
  2. 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ)
  4. no protein annotated in this organism
  5. Shikimate kinase (aroK), Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC), Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40NADPUniRule annotation1 Publication1
Binding sitei46NADPUniRule annotation1 Publication1
Binding sitei300FMN; via amide nitrogenUniRule annotation1
Binding sitei341FMNUniRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi135 – 137FMNUniRule annotation3
Nucleotide bindingi256 – 257FMNUniRule annotation1 Publication2
Nucleotide bindingi315 – 319FMNUniRule annotation1 Publication5

GO - Molecular functioni

  • chorismate synthase activity Source: MTBBASE
  • FMN binding Source: MTBBASE
  • NAD binding Source: MTBBASE
  • oxidoreductase activity, acting on NAD(P)H Source: MTBBASE

GO - Biological processi

  • aromatic amino acid family biosynthetic process Source: GO_Central
  • chorismate biosynthetic process Source: GO_Central
  • growth Source: MTBBASE

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandFAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

ReactomeiR-MTU-964903 Chorismate via Shikimate Pathway
UniPathwayiUPA00053; UER00090

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthaseUniRule annotation (EC:4.2.3.5UniRule annotation)
Short name:
CS1 PublicationUniRule annotation
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyaseUniRule annotation
Gene namesi
Name:aroCUniRule annotation
Synonyms:aroF
Ordered Locus Names:Rv2540c
ORF Names:MTCY159.16
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2540c

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001406151 – 401Chorismate synthaseAdd BLAST401

Proteomic databases

PaxDbiP9WPY1
PRIDEiP9WPY1

Interactioni

Subunit structurei

Homotetramer: dimer of dimers.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi83332.Rv2540c

Structurei

Secondary structure

1401
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Beta strandi12 – 21Combined sources10
Beta strandi24 – 27Combined sources4
Helixi30 – 40Combined sources11
Helixi46 – 48Combined sources3
Helixi54 – 56Combined sources3
Beta strandi57 – 64Combined sources8
Beta strandi67 – 71Combined sources5
Beta strandi73 – 78Combined sources6
Helixi82 – 85Combined sources4
Turni86 – 89Combined sources4
Helixi96 – 100Combined sources5
Helixi103 – 105Combined sources3
Helixi117 – 124Combined sources8
Beta strandi127 – 129Combined sources3
Helixi130 – 136Combined sources7
Helixi138 – 140Combined sources3
Helixi141 – 159Combined sources19
Beta strandi162 – 170Combined sources9
Helixi183 – 185Combined sources3
Helixi186 – 191Combined sources6
Beta strandi196 – 198Combined sources3
Helixi199 – 215Combined sources17
Beta strandi221 – 229Combined sources9
Beta strandi237 – 239Combined sources3
Helixi240 – 242Combined sources3
Helixi244 – 253Combined sources10
Beta strandi258 – 263Combined sources6
Helixi266 – 269Combined sources4
Helixi274 – 277Combined sources4
Beta strandi289 – 291Combined sources3
Turni294 – 297Combined sources4
Beta strandi308 – 314Combined sources7
Turni321 – 323Combined sources3
Beta strandi325 – 327Combined sources3
Turni329 – 331Combined sources3
Beta strandi334 – 336Combined sources3
Helixi347 – 370Combined sources24
Helixi375 – 390Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZTBX-ray2.65A1-401[»]
2G85X-ray2.22A1-401[»]
2O11X-ray1.65A1-401[»]
2O12X-ray1.72A1-401[»]
2QHFX-ray1.65A1-401[»]
4BAIX-ray2.30A1-401[»]
4BAJX-ray2.30A1-401[»]
ProteinModelPortaliP9WPY1
SMRiP9WPY1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D10 Bacteria
COG0082 LUCA
KOiK01736
OMAiMLSINAV
PhylomeDBiP9WPY1

Family and domain databases

CDDicd07304 Chorismate_synthase, 1 hit
Gene3Di3.60.150.10, 1 hit
HAMAPiMF_00300 Chorismate_synth, 1 hit
InterProiView protein in InterPro
IPR000453 Chorismate_synth
IPR035904 Chorismate_synth_AroC_sf
IPR020541 Chorismate_synthase_CS
PANTHERiPTHR21085 PTHR21085, 1 hit
PfamiView protein in Pfam
PF01264 Chorismate_synt, 1 hit
PIRSFiPIRSF001456 Chorismate_synth, 1 hit
SUPFAMiSSF103263 SSF103263, 1 hit
TIGRFAMsiTIGR00033 aroC, 1 hit
PROSITEiView protein in PROSITE
PS00787 CHORISMATE_SYNTHASE_1, 1 hit
PS00788 CHORISMATE_SYNTHASE_2, 1 hit
PS00789 CHORISMATE_SYNTHASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

P9WPY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRWITAGES HGRALVAVVE GMVAGVHVTS ADIADQLARR RLGYGRGARM
60 70 80 90 100
TFERDAVTVL SGIRHGSTLG GPIAIEIGNT EWPKWETVMA ADPVDPAELA
110 120 130 140 150
DVARNAPLTR PRPGHADYAG MLKYGFDDAR PVLERASARE TAARVAAGTV
160 170 180 190 200
ARAFLRQALG VEVLSHVISI GASAPYEGPP PRAEDLPAID ASPVRAYDKA
210 220 230 240 250
AEADMIAQIE AAKKDGDTLG GVVEAVALGL PVGLGSFTSG DHRLDSQLAA
260 270 280 290 300
AVMGIQAIKG VEIGDGFQTA RRRGSRAHDE MYPGPDGVVR STNRAGGLEG
310 320 330 340 350
GMTNGQPLRV RAAMKPISTV PRALATVDLA TGDEAVAIHQ RSDVCAVPAA
360 370 380 390 400
GVVVETMVAL VLARAALEKF GGDSLAETQR NIAAYQRSVA DREAPAARVS

G
Length:401
Mass (Da):41,792
Last modified:April 16, 2014 - v1
Checksum:i6A3C14761261ADEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA Translation: CCP45335.1
PIRiH70658
RefSeqiNP_217056.1, NC_000962.3
WP_003413027.1, NZ_KK339370.1

Genome annotation databases

EnsemblBacteriaiCCP45335; CCP45335; Rv2540c
GeneIDi887379
KEGGimtu:Rv2540c

Similar proteinsi

Entry informationi

Entry nameiAROC_MYCTU
AccessioniPrimary (citable) accession number: P9WPY1
Secondary accession number(s): L0T9X6, P63611, P95013
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: May 23, 2018
This is version 25 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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