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Protein

10 kDa chaperonin

Gene

groS

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.

GO - Molecular functioni

  • ATP binding Source: InterPro
  • chaperone binding Source: GO_Central
  • metal ion binding Source: MTBBASE
  • unfolded protein binding Source: GO_Central

GO - Biological processi

  • cellular response to heat Source: MTBBASE
  • chaperone mediated protein folding requiring cofactor Source: GO_Central
  • growth Source: MTBBASE
  • regulation of transcription, DNA-templated Source: MTBBASE
  • response to antibiotic Source: MTBBASE
  • response to heat Source: MTBBASE
  • response to unfolded protein Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
10 kDa chaperonin
Alternative name(s):
10 kDa antigen
BCG-A heat shock protein
GroES protein
Protein Cpn10
Gene namesi
Name:groS
Synonyms:cpn10, groES, mopB
Ordered Locus Names:Rv3418c
ORF Names:MTCY78.11
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv3418c.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • extracellular region Source: MTBBASE
  • GroEL-GroES complex Source: GO_Central
  • plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources1 Publication
Chaini2 – 1009910 kDa chaperoninPRO_0000174788Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Cross-linki100 – 100Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP9WPE5.

Interactioni

Subunit structurei

Heptamer of 7 subunits arranged in a ring.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi83332.Rv3418c.

Structurei

Secondary structure

1
100
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 84Combined sources
Beta strandi12 – 176Combined sources
Beta strandi19 – 246Combined sources
Turni25 – 273Combined sources
Beta strandi37 – 4610Combined sources
Beta strandi53 – 564Combined sources
Beta strandi68 – 725Combined sources
Beta strandi77 – 815Combined sources
Beta strandi84 – 907Combined sources
Helixi91 – 933Combined sources
Beta strandi94 – 996Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HX5X-ray3.50A/B/C/D/E/F/G2-100[»]
1P3HX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L/M/N2-100[»]
1P82NMR-A2-26[»]
1P83NMR-A2-26[»]
ProteinModelPortaliP9WPE5.
SMRiP9WPE5. Positions 2-100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GroES chaperonin family.Curated

Phylogenomic databases

eggNOGiENOG4105K5Y. Bacteria.
COG0234. LUCA.
KOiK04078.
OMAiEHESCCH.
PhylomeDBiP9WPE5.

Family and domain databases

Gene3Di2.30.33.40. 1 hit.
HAMAPiMF_00580. CH10.
InterProiIPR020818. Chaperonin_GroES.
IPR018369. Chaprnonin_Cpn10_CS.
IPR011032. GroES-like.
[Graphical view]
PANTHERiPTHR10772. PTHR10772. 1 hit.
PfamiPF00166. Cpn10. 1 hit.
[Graphical view]
PRINTSiPR00297. CHAPERONIN10.
SMARTiSM00883. Cpn10. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
PROSITEiPS00681. CHAPERONINS_CPN10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WPE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVNIKPLE DKILVQANEA ETTTASGLVI PDTAKEKPQE GTVVAVGPGR
60 70 80 90 100
WDEDGEKRIP LDVAEGDTVI YSKYGGTEIK YNGEEYLILS ARDVLAVVSK
Length:100
Mass (Da):10,804
Last modified:April 16, 2014 - v1
Checksum:iDE448187A56103FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60350 Genomic DNA. Translation: CAA42908.1.
M25258 Genomic DNA. Translation: AAA25340.1.
X13739 Genomic DNA. Translation: CAA32003.1.
AL123456 Genomic DNA. Translation: CCP46240.1.
PIRiS01381. BVMYBA.
RefSeqiNP_217935.1. NC_000962.3.
WP_003418028.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP46240; CCP46240; Rv3418c.
GeneIDi887583.
KEGGimtu:Rv3418c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60350 Genomic DNA. Translation: CAA42908.1.
M25258 Genomic DNA. Translation: AAA25340.1.
X13739 Genomic DNA. Translation: CAA32003.1.
AL123456 Genomic DNA. Translation: CCP46240.1.
PIRiS01381. BVMYBA.
RefSeqiNP_217935.1. NC_000962.3.
WP_003418028.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HX5X-ray3.50A/B/C/D/E/F/G2-100[»]
1P3HX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L/M/N2-100[»]
1P82NMR-A2-26[»]
1P83NMR-A2-26[»]
ProteinModelPortaliP9WPE5.
SMRiP9WPE5. Positions 2-100.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv3418c.

Proteomic databases

PaxDbiP9WPE5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP46240; CCP46240; Rv3418c.
GeneIDi887583.
KEGGimtu:Rv3418c.

Organism-specific databases

TubercuListiRv3418c.

Phylogenomic databases

eggNOGiENOG4105K5Y. Bacteria.
COG0234. LUCA.
KOiK04078.
OMAiEHESCCH.
PhylomeDBiP9WPE5.

Family and domain databases

Gene3Di2.30.33.40. 1 hit.
HAMAPiMF_00580. CH10.
InterProiIPR020818. Chaperonin_GroES.
IPR018369. Chaprnonin_Cpn10_CS.
IPR011032. GroES-like.
[Graphical view]
PANTHERiPTHR10772. PTHR10772. 1 hit.
PfamiPF00166. Cpn10. 1 hit.
[Graphical view]
PRINTSiPR00297. CHAPERONIN10.
SMARTiSM00883. Cpn10. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
PROSITEiPS00681. CHAPERONINS_CPN10. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A major antigen from Mycobacterium tuberculosis which is homologous to the heat shock proteins groES from E. coli and the htpA gene product of Coxiella burneti."
    Baird P.N., Hall L.M., Coates A.R.M.
    Nucleic Acids Res. 16:9047-9047(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. "Cloning and sequence analysis of the 10 kDa antigen gene of Mycobacterium tuberculosis."
    Baird P.N., Hall L.M.C., Coates A.R.M.
    J. Gen. Microbiol. 135:931-939(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  3. "The Mycobacterium tuberculosis BCG-a protein has homology with the Escherichia coli GroES protein."
    Shinnick T.M., Plikaytis B.P., Hyche A.D., van Landingham R.M., Walker L.L.
    Nucleic Acids Res. 17:1254-1254(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 35801 / TMC 107 / Erdman.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 35801 / TMC 107 / Erdman.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  6. "Immunoreactivity of a 10-kDa antigen of Mycobacterium tuberculosis."
    Barnes P.F., Mehra V., Rivoire B., Fong S.J., Brennan P.J., Voegtline M.S., Minden P., Houghten R.A., Bloom B.R., Modlin R.L.
    J. Immunol. 148:1835-1840(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-16.
  7. "Prokayrotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis."
    Festa R.A., McAllister F., Pearce M.J., Mintseris J., Burns K.E., Gygi S.P., Darwin K.H.
    PLoS ONE 5:E8589-E8589(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PUPYLATION AT LYS-100, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: ATCC 25618 / H37Rv.
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiCH10_MYCTU
AccessioniPrimary (citable) accession number: P9WPE5
Secondary accession number(s): L0TFJ8, P09621
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: June 8, 2016
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.