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Protein

Phosphopantetheine adenylyltransferase

Gene

coaD

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.UniRule annotation

Catalytic activityi

ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA.UniRule annotation

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes CoA from (R)-pantothenate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pantothenate kinase (coaA), Type III pantothenate kinase (coaX), Pantothenate kinase (coaA), Type III pantothenate kinase (coaX)
  2. Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  3. Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  4. Phosphopantetheine adenylyltransferase (coaD), Phosphopantetheine adenylyltransferase (coaD)
  5. Dephospho-CoA kinase (coaE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • pantetheine-phosphate adenylyltransferase activity Source: MTBBASE

GO - Biological processi

  • coenzyme A biosynthetic process Source: MTBBASE
  • protein hexamerization Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Coenzyme A biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-7219-MONOMER.
UniPathwayiUPA00241; UER00355.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphopantetheine adenylyltransferaseUniRule annotation (EC:2.7.7.3UniRule annotation)
Alternative name(s):
Dephospho-CoA pyrophosphorylaseUniRule annotation
Pantetheine-phosphate adenylyltransferaseUniRule annotation
Short name:
PPATUniRule annotation
Gene namesi
Name:coaDUniRule annotation
Synonyms:kdtB
Ordered Locus Names:Rv2965c
ORF Names:MTCY349.22, u0002e
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2965c.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001562422 – 161Phosphopantetheine adenylyltransferaseAdd BLAST160

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP9WPA5.

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

STRINGi83332.Rv2965c.

Structurei

Secondary structure

1161
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi15 – 27Combined sources13
Beta strandi28 – 35Combined sources8
Beta strandi39 – 41Combined sources3
Helixi47 – 57Combined sources11
Beta strandi64 – 68Combined sources5
Helixi73 – 79Combined sources7
Beta strandi84 – 89Combined sources6
Helixi95 – 109Combined sources15
Beta strandi112 – 117Combined sources6
Helixi120 – 122Combined sources3
Helixi127 – 135Combined sources9
Helixi141 – 143Combined sources3
Helixi146 – 156Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TFUX-ray1.99A1-157[»]
3LCJX-ray2.10A1-161[»]
3NBAX-ray2.68A/B/C/D1-157[»]
3NBKX-ray1.58A/B/C/D1-157[»]
3PNBX-ray2.11A/B/C/D1-157[»]
3RBAX-ray1.59A1-157[»]
3RFFX-ray1.76A1-157[»]
3RHSX-ray1.59A1-157[»]
3UC5X-ray1.70A1-157[»]
4E1AX-ray1.62A1-161[»]
4R0NX-ray2.00A/C/E/G/I/K2-157[»]
ProteinModelPortaliP9WPA5.
SMRiP9WPA5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial CoaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108ZEF. Bacteria.
COG0669. LUCA.
KOiK00954.
OMAiEFQMALM.
PhylomeDBiP9WPA5.

Family and domain databases

CDDicd02163. PPAT. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00151. PPAT_bact. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR001980. LPS_biosynth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
PRINTSiPR01020. LPSBIOSNTHSS.
TIGRFAMsiTIGR01510. coaD_prev_kdtB. 1 hit.
TIGR00125. cyt_tran_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WPA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGAVCPGSF DPVTLGHVDI FERAAAQFDE VVVAILVNPA KTGMFDLDER
60 70 80 90 100
IAMVKESTTH LPNLRVQVGH GLVVDFVRSC GMTAIVKGLR TGTDFEYELQ
110 120 130 140 150
MAQMNKHIAG VDTFFVATAP RYSFVSSSLA KEVAMLGGDV SELLPEPVNR
160
RLRDRLNTER T
Length:161
Mass (Da):17,628
Last modified:April 16, 2014 - v1
Checksum:iDE9F12E18D0C1721
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00024 Genomic DNA. Translation: AAA50946.1.
AL123456 Genomic DNA. Translation: CCP45769.1.
PIRiB70671.
RefSeqiNP_217481.1. NC_000962.3.
WP_003414998.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP45769; CCP45769; Rv2965c.
GeneIDi888423.
KEGGimtu:Rv2965c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00024 Genomic DNA. Translation: AAA50946.1.
AL123456 Genomic DNA. Translation: CCP45769.1.
PIRiB70671.
RefSeqiNP_217481.1. NC_000962.3.
WP_003414998.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TFUX-ray1.99A1-157[»]
3LCJX-ray2.10A1-161[»]
3NBAX-ray2.68A/B/C/D1-157[»]
3NBKX-ray1.58A/B/C/D1-157[»]
3PNBX-ray2.11A/B/C/D1-157[»]
3RBAX-ray1.59A1-157[»]
3RFFX-ray1.76A1-157[»]
3RHSX-ray1.59A1-157[»]
3UC5X-ray1.70A1-157[»]
4E1AX-ray1.62A1-161[»]
4R0NX-ray2.00A/C/E/G/I/K2-157[»]
ProteinModelPortaliP9WPA5.
SMRiP9WPA5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv2965c.

Proteomic databases

PaxDbiP9WPA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP45769; CCP45769; Rv2965c.
GeneIDi888423.
KEGGimtu:Rv2965c.

Organism-specific databases

TubercuListiRv2965c.

Phylogenomic databases

eggNOGiENOG4108ZEF. Bacteria.
COG0669. LUCA.
KOiK00954.
OMAiEFQMALM.
PhylomeDBiP9WPA5.

Enzyme and pathway databases

UniPathwayiUPA00241; UER00355.
BioCyciMTBH37RV:G185E-7219-MONOMER.

Family and domain databases

CDDicd02163. PPAT. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00151. PPAT_bact. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR001980. LPS_biosynth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
PRINTSiPR01020. LPSBIOSNTHSS.
TIGRFAMsiTIGR01510. coaD_prev_kdtB. 1 hit.
TIGR00125. cyt_tran_rel. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOAD_MYCTU
AccessioniPrimary (citable) accession number: P9WPA5
Secondary accession number(s): L0TCR8
, O08023, P0A530, Q50452
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 2, 2016
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.