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Protein

Phosphopantetheine adenylyltransferase

Gene

coaD

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.UniRule annotation

Catalytic activityi

ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA.UniRule annotation

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes CoA from (R)-pantothenate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pantothenate kinase (coaA), Type III pantothenate kinase (coaX), Pantothenate kinase (coaA), Type III pantothenate kinase (coaX)
  2. Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  3. Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  4. Phosphopantetheine adenylyltransferase (coaD), Phosphopantetheine adenylyltransferase (coaD)
  5. Dephospho-CoA kinase (coaE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • pantetheine-phosphate adenylyltransferase activity Source: MTBBASE

GO - Biological processi

  • coenzyme A biosynthetic process Source: MTBBASE
  • protein hexamerization Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Coenzyme A biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00241; UER00355.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphopantetheine adenylyltransferaseUniRule annotation (EC:2.7.7.3UniRule annotation)
Alternative name(s):
Dephospho-CoA pyrophosphorylaseUniRule annotation
Pantetheine-phosphate adenylyltransferaseUniRule annotation
Short name:
PPATUniRule annotation
Gene namesi
Name:coaDUniRule annotation
Synonyms:kdtB
Ordered Locus Names:Rv2965c
ORF Names:MTCY349.22, u0002e
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2965c.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 161160Phosphopantetheine adenylyltransferasePRO_0000156242Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP9WPA5.

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

STRINGi83332.Rv2965c.

Structurei

Secondary structure

1
161
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi15 – 2713Combined sources
Beta strandi28 – 358Combined sources
Beta strandi39 – 413Combined sources
Helixi47 – 5711Combined sources
Beta strandi64 – 685Combined sources
Helixi73 – 797Combined sources
Beta strandi84 – 896Combined sources
Helixi95 – 10915Combined sources
Beta strandi112 – 1176Combined sources
Helixi120 – 1223Combined sources
Helixi127 – 1359Combined sources
Helixi141 – 1433Combined sources
Helixi146 – 15611Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TFUX-ray1.99A1-157[»]
3LCJX-ray2.10A1-161[»]
3NBAX-ray2.68A/B/C/D1-157[»]
3NBKX-ray1.58A/B/C/D1-157[»]
3PNBX-ray2.11A/B/C/D1-157[»]
3RBAX-ray1.59A1-157[»]
3RFFX-ray1.76A1-157[»]
3RHSX-ray1.59A1-157[»]
3UC5X-ray1.70A1-157[»]
4E1AX-ray1.62A1-161[»]
4R0NX-ray2.00A/C/E/G/I/K2-157[»]
ProteinModelPortaliP9WPA5.
SMRiP9WPA5. Positions 1-157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial CoaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108ZEF. Bacteria.
COG0669. LUCA.
KOiK00954.
OMAiYELQIAH.
PhylomeDBiP9WPA5.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00151. PPAT_bact.
InterProiIPR004821. Cyt_trans-like.
IPR001980. LPS_biosynth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
PRINTSiPR01020. LPSBIOSNTHSS.
TIGRFAMsiTIGR01510. coaD_prev_kdtB. 1 hit.
TIGR00125. cyt_tran_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WPA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGAVCPGSF DPVTLGHVDI FERAAAQFDE VVVAILVNPA KTGMFDLDER
60 70 80 90 100
IAMVKESTTH LPNLRVQVGH GLVVDFVRSC GMTAIVKGLR TGTDFEYELQ
110 120 130 140 150
MAQMNKHIAG VDTFFVATAP RYSFVSSSLA KEVAMLGGDV SELLPEPVNR
160
RLRDRLNTER T
Length:161
Mass (Da):17,628
Last modified:April 16, 2014 - v1
Checksum:iDE9F12E18D0C1721
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00024 Genomic DNA. Translation: AAA50946.1.
AL123456 Genomic DNA. Translation: CCP45769.1.
PIRiB70671.
RefSeqiNP_217481.1. NC_000962.3.
WP_003414998.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP45769; CCP45769; Rv2965c.
GeneIDi888423.
KEGGimtu:Rv2965c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00024 Genomic DNA. Translation: AAA50946.1.
AL123456 Genomic DNA. Translation: CCP45769.1.
PIRiB70671.
RefSeqiNP_217481.1. NC_000962.3.
WP_003414998.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TFUX-ray1.99A1-157[»]
3LCJX-ray2.10A1-161[»]
3NBAX-ray2.68A/B/C/D1-157[»]
3NBKX-ray1.58A/B/C/D1-157[»]
3PNBX-ray2.11A/B/C/D1-157[»]
3RBAX-ray1.59A1-157[»]
3RFFX-ray1.76A1-157[»]
3RHSX-ray1.59A1-157[»]
3UC5X-ray1.70A1-157[»]
4E1AX-ray1.62A1-161[»]
4R0NX-ray2.00A/C/E/G/I/K2-157[»]
ProteinModelPortaliP9WPA5.
SMRiP9WPA5. Positions 1-157.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv2965c.

Proteomic databases

PaxDbiP9WPA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP45769; CCP45769; Rv2965c.
GeneIDi888423.
KEGGimtu:Rv2965c.

Organism-specific databases

TubercuListiRv2965c.

Phylogenomic databases

eggNOGiENOG4108ZEF. Bacteria.
COG0669. LUCA.
KOiK00954.
OMAiYELQIAH.
PhylomeDBiP9WPA5.

Enzyme and pathway databases

UniPathwayiUPA00241; UER00355.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00151. PPAT_bact.
InterProiIPR004821. Cyt_trans-like.
IPR001980. LPS_biosynth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
PRINTSiPR01020. LPSBIOSNTHSS.
TIGRFAMsiTIGR01510. coaD_prev_kdtB. 1 hit.
TIGR00125. cyt_tran_rel. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Smith D.R., Robison K.
    Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  3. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiCOAD_MYCTU
AccessioniPrimary (citable) accession number: P9WPA5
Secondary accession number(s): L0TCR8
, O08023, P0A530, Q50452
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 11, 2015
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.