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P9WP29

- DAGK_MYCTU

UniProt

P9WP29 - DAGK_MYCTU

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Protein

Diacylglycerol kinase

Gene

dagK

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is involved in the biosynthesis of phosphatidylinositol mannosides (PIMs), probably via a role in the biosynthesis of phosphatidylinositol (PI), a PIM precursor, which is derived from phosphatidic acid (By similarity).By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Cofactori

Binds 1 magnesium ion per subunit. This ion appears to have a structural role and is required for catalytic activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011ATPPROSITE-ProRule annotation
Metal bindingi226 – 2261Magnesium; via carbonyl oxygenBy similarity
Metal bindingi229 – 2291MagnesiumBy similarity
Metal bindingi231 – 2311Magnesium; via carbonyl oxygenBy similarity
Active sitei285 – 2851Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 235ATPPROSITE-ProRule annotation
Nucleotide bindingi76 – 827ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: MTBBASE
  3. metal ion binding Source: UniProtKB-KW
  4. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. glycolipid biosynthetic process Source: MTBBASE
  2. phospholipid biosynthetic process Source: UniProtKB-KW
  3. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAG kinase
Short name:
DAGK
Gene namesi
Name:dagK
Ordered Locus Names:Rv2252
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001584: Chromosome

Organism-specific databases

TubercuListiRv2252.

Subcellular locationi

Secretedcell wall Curated

GO - Cellular componenti

  1. cell wall Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
  3. plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Diacylglycerol kinasePRO_0000386484Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP9WP29.
SMRiP9WP29. Positions 34-306.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 140132DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the diacylglycerol/lipid kinase family.Curated
Contains 1 DAGKc domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK07029.
PhylomeDBiP9WP29.

Family and domain databases

InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WP29-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAGQLRRHE IGKVTALTNP LSGHGAAVKA AHGAIARLKH RGVDVVEIVG
60 70 80 90 100
GDAHDARHLL AAAVAKGTDA VMVTGGDGVV SNALQVLAGT DIPLGIIPAG
110 120 130 140 150
TGNDHAREFG LPTKNPKAAA DIVVDGWTET IDLGRIQDDN GIEKWFGTVA
160 170 180 190 200
ATGFDSLVND RANRMRWPHG RMRYYIAMLA ELSRLRPLPF RLVLDGTEEI
210 220 230 240 250
VADLTLADFG NTRSYGGGLL ICPNADHSDG LLDITMAQSD SRTKLLRLFP
260 270 280 290 300
TIFKGAHVEL DEVSTTRAKT VHVECPGINV YADGDFACPL PAEISAVPAA

LQVLRPRHG
Length:309
Mass (Da):32,805
Last modified:April 16, 2014 - v1
Checksum:i34F5E363D62BB284
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL123456 Genomic DNA. Translation: CCP45033.1.
PIRiH70861.
RefSeqiNP_216768.1. NC_000962.3.
YP_006515675.1. NC_018143.2.

Genome annotation databases

GeneIDi13318945.
888429.
KEGGimtu:Rv2252.
mtv:RVBD_2252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL123456 Genomic DNA. Translation: CCP45033.1 .
PIRi H70861.
RefSeqi NP_216768.1. NC_000962.3.
YP_006515675.1. NC_018143.2.

3D structure databases

ProteinModelPortali P9WP29.
SMRi P9WP29. Positions 34-306.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13318945.
888429.
KEGGi mtu:Rv2252.
mtv:RVBD_2252.

Organism-specific databases

TubercuListi Rv2252.

Phylogenomic databases

KOi K07029.
PhylomeDBi P9WP29.

Family and domain databases

InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
[Graphical view ]
Pfami PF00781. DAGK_cat. 1 hit.
[Graphical view ]
SMARTi SM00046. DAGKc. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
TIGRFAMsi TIGR00147. TIGR00147. 1 hit.
PROSITEi PS50146. DAGK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiDAGK_MYCTU
AccessioniPrimary (citable) accession number: P9WP29
Secondary accession number(s): L0TAM7, O53526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: October 29, 2014
This is version 7 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3