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P9WP29

- DAGK_MYCTU

UniProt

P9WP29 - DAGK_MYCTU

Protein

Diacylglycerol kinase

Gene

dagK

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 6 (01 Oct 2014)
      Sequence version 1 (16 Apr 2014)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is involved in the biosynthesis of phosphatidylinositol mannosides (PIMs), probably via a role in the biosynthesis of phosphatidylinositol (PI), a PIM precursor, which is derived from phosphatidic acid By similarity.By similarity

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit. This ion appears to have a structural role and is required for catalytic activity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei101 – 1011ATPPROSITE-ProRule annotation
    Metal bindingi226 – 2261Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi229 – 2291MagnesiumBy similarity
    Metal bindingi231 – 2311Magnesium; via carbonyl oxygenBy similarity
    Active sitei285 – 2851Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 235ATPPROSITE-ProRule annotation
    Nucleotide bindingi76 – 827ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. phospholipid biosynthetic process Source: UniProtKB-KW
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase (EC:2.7.1.107)
    Short name:
    DAG kinase
    Short name:
    DAGK
    Gene namesi
    Name:dagK
    Ordered Locus Names:Rv2252
    OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    Taxonomic identifieri83332 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001584: Chromosome

    Organism-specific databases

    TubercuListiRv2252.

    Subcellular locationi

    Secretedcell wall Curated

    GO - Cellular componenti

    1. cell wall Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-KW
    3. plasma membrane Source: MTBBASE

    Keywords - Cellular componenti

    Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Diacylglycerol kinasePRO_0000386484Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP9WP29.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 140132DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the diacylglycerol/lipid kinase family.Curated
    Contains 1 DAGKc domain.PROSITE-ProRule annotation

    Phylogenomic databases

    KOiK07029.
    PhylomeDBiP9WP29.

    Family and domain databases

    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR005218. Diacylglycerol/lipid_kinase.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    [Graphical view]
    PfamiPF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
    PROSITEiPS50146. DAGK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P9WP29-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAGQLRRHE IGKVTALTNP LSGHGAAVKA AHGAIARLKH RGVDVVEIVG    50
    GDAHDARHLL AAAVAKGTDA VMVTGGDGVV SNALQVLAGT DIPLGIIPAG 100
    TGNDHAREFG LPTKNPKAAA DIVVDGWTET IDLGRIQDDN GIEKWFGTVA 150
    ATGFDSLVND RANRMRWPHG RMRYYIAMLA ELSRLRPLPF RLVLDGTEEI 200
    VADLTLADFG NTRSYGGGLL ICPNADHSDG LLDITMAQSD SRTKLLRLFP 250
    TIFKGAHVEL DEVSTTRAKT VHVECPGINV YADGDFACPL PAEISAVPAA 300
    LQVLRPRHG 309
    Length:309
    Mass (Da):32,805
    Last modified:April 16, 2014 - v1
    Checksum:i34F5E363D62BB284
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL123456 Genomic DNA. Translation: CCP45033.1.
    PIRiH70861.
    RefSeqiNP_216768.1. NC_000962.3.
    YP_006515675.1. NC_018143.2.

    Genome annotation databases

    GeneIDi13318945.
    888429.
    KEGGimtu:Rv2252.
    mtv:RVBD_2252.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL123456 Genomic DNA. Translation: CCP45033.1 .
    PIRi H70861.
    RefSeqi NP_216768.1. NC_000962.3.
    YP_006515675.1. NC_018143.2.

    3D structure databases

    ProteinModelPortali P9WP29.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13318945.
    888429.
    KEGGi mtu:Rv2252.
    mtv:RVBD_2252.

    Organism-specific databases

    TubercuListi Rv2252.

    Phylogenomic databases

    KOi K07029.
    PhylomeDBi P9WP29.

    Family and domain databases

    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR005218. Diacylglycerol/lipid_kinase.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    [Graphical view ]
    Pfami PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    TIGRFAMsi TIGR00147. TIGR00147. 1 hit.
    PROSITEi PS50146. DAGK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25618 / H37Rv.
    2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 25618 / H37Rv.

    Entry informationi

    Entry nameiDAGK_MYCTU
    AccessioniPrimary (citable) accession number: P9WP29
    Secondary accession number(s): L0TAM7, O53526
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 16, 2014
    Last modified: October 1, 2014
    This is version 6 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3